BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3521.p (613 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095607|ref|YP_001100682.1| GTP-binding elongation factor f... 1172 0.0 gi|134095256|ref|YP_001100331.1| GTP-binding protein LepA [Hermi... 159 3e-40 gi|134094178|ref|YP_001099253.1| peptide chain release factor 3 ... 90 4e-19 gi|134096324|ref|YP_001101399.1| elongation factor G [Herminiimo... 89 5e-19 gi|134096335|ref|YP_001101410.1| elongation factor Tu [Herminiim... 82 8e-17 >gi|134095607|ref|YP_001100682.1| GTP-binding elongation factor family protein [Herminiimonas arsenicoxydans] Length = 612 Score = 1172 bits (3033), Expect = 0.0, Method: Compositional matrix adjust. Identities = 569/612 (92%), Positives = 595/612 (97%) Query: 1 MSNNIRAIRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQVDNRVMDSNDIEKERGITIL 60 MSN+ RAIRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQ+D RVMDS DIEKERGITIL Sbjct: 1 MSNSKRAIRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQIDARVMDSGDIEKERGITIL 60 Query: 61 SKNCAVEYKGTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKAL 120 SKNCAVEYKGTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKAL Sbjct: 61 SKNCAVEYKGTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKAL 120 Query: 121 ALGLKPIVVLNKIDRPGARAEWAINATFELFDKLGATEEQLDFPVIYASGLNGYASLDPE 180 ALGLKPIVVLNKIDRPGARAEWAINATFELFDKLGATEEQLDFP++YASGLNGYA L P+ Sbjct: 121 ALGLKPIVVLNKIDRPGARAEWAINATFELFDKLGATEEQLDFPIVYASGLNGYAGLTPD 180 Query: 181 TREGNMEPLFDAILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRIKGGQDV 240 R GNMEPLFDAIL++VPAR+DDPDAPLQLQITSLEYSSYVGKIGVGRILAGR+K QDV Sbjct: 181 VRGGNMEPLFDAILEHVPARQDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRVKALQDV 240 Query: 241 VWMNGPEDKPTKARINQVLTFKGLERVLVDEALAGDIVLINGIEEIGIGSTICAPDAPNG 300 VWMNGP+DKPTKARINQVLTFKGL+RVLVDEALAGDIVLINGIEEIGIG+TICAPD PNG Sbjct: 241 VWMNGPDDKPTKARINQVLTFKGLDRVLVDEALAGDIVLINGIEEIGIGTTICAPDHPNG 300 Query: 301 LPMLKVDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNMALRVVQAENDDST 360 LPMLKVDEPTLTMNFMVN SPLAGREGKFVTTRQIRDRLE+ELK+NMALRVVQ+ENDDST Sbjct: 301 LPMLKVDEPTLTMNFMVNNSPLAGREGKFVTTRQIRDRLERELKANMALRVVQSENDDST 360 Query: 361 YEVSGRGELHLTILIENMRREGFELAVSRPRVVFRMVDGVREEPYENLTVDVEETHQGGV 420 YEVSGRGELHLTILIENMRREGFELAVSRPRVVF+MVDGVR EP+ENLT+DVEET+QGGV Sbjct: 361 YEVSGRGELHLTILIENMRREGFELAVSRPRVVFKMVDGVRHEPFENLTIDVEETNQGGV 420 Query: 421 MEELGRRRGDLQNMEPDGKGRVRLEYHIPARGLIGFQGEFMTLTRGTGLMSHVFDDYKPV 480 MEELGRRRGDLQNMEPDGKGRVRLEYHIPARGLIGFQGEFMTLTRGTGLMSHVFDDY PV Sbjct: 421 MEELGRRRGDLQNMEPDGKGRVRLEYHIPARGLIGFQGEFMTLTRGTGLMSHVFDDYAPV 480 Query: 481 DTSKGELAGRRNGVLISQDDGAAVAYALWKLQDRGRMFVSHNDPVYEGMIIGIHSRDNDL 540 D SKGEL GRRNGVLISQDDGAAVAYA+WKLQDRGRMFVSHNDPVYEGMIIGIHSRDNDL Sbjct: 481 DVSKGELGGRRNGVLISQDDGAAVAYAIWKLQDRGRMFVSHNDPVYEGMIIGIHSRDNDL 540 Query: 541 VVNPIKGKQLTNVRASGTDEAVRLVPPIELSLEYAVEFIEDDELVEVTPKSIRLRKRHLK 600 VVNPIKGKQLTNVR+SGTDEAVRLVPPIE+SLEYAVEFI+DDELVEVTPKSIRLRKR+LK Sbjct: 541 VVNPIKGKQLTNVRSSGTDEAVRLVPPIEMSLEYAVEFIDDDELVEVTPKSIRLRKRYLK 600 Query: 601 EHERKKASREES 612 EHERKKA R+ + Sbjct: 601 EHERKKAGRDAA 612 >gi|134095256|ref|YP_001100331.1| GTP-binding protein LepA [Herminiimonas arsenicoxydans] Length = 597 Score = 159 bits (402), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 159/551 (28%), Positives = 254/551 (46%), Gaps = 79/551 (14%) Query: 8 IRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVE 67 IRN +IIAH+DHGK+TL D++++ G D ++++ +V+DS D+E+ERGITI ++ A+ Sbjct: 4 IRNFSIIAHIDHGKSTLADRIIQLCGGLSD-REMEAQVLDSMDLERERGITIKAQTAALT 62 Query: 68 YKG-----THINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALAL 122 YK ++N++DTPGH DF EV R LS + LL+VDA +G QT AL L Sbjct: 63 YKARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYMALDL 122 Query: 123 GLKPIVVLNKIDRPGARAEWAINATFELFDKLGATEEQLDFPVIYASGLNGYASLDPETR 182 G++ + VLNKID P A A+ A E + AT+ + S G D Sbjct: 123 GVEVVPVLNKIDLPNADPPAAM-AEIEDVIGIDATD------AVQCSAKTGLGVAD---- 171 Query: 183 EGNMEPLFDAILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRIKGGQDVVW 242 + +A++ VP K DPDAPLQ I + +YVG + + R++ G +K + + Sbjct: 172 ------VLEALIAKVPPPKGDPDAPLQALIVDSWFDNYVGVVMLVRVVNGTLKPKEKIRL 225 Query: 243 MNGPEDKPTKARINQV--LTFKGLERVLVDEALAGDI-VLINGIEE-----IGIGSTICA 294 M +N V + V + AG + +I GI+E +G T+ + Sbjct: 226 M-------ASGSVNLVESIGIFAPRSVSLPSLSAGQVGFIIAGIKELKAAKVGDTVTLAS 278 Query: 295 PDAPNGLPMLKVDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNMALRVVQA 354 A LP K +P + P+ + +RD LEK LK N A + + Sbjct: 279 RPAAEALPGFKEVQPQVFAGLF----PVEANQ-----YDALRDSLEK-LKLNDAALMYEP 328 Query: 355 ENDDST---YEVSGRGELHLTILIENMRRE-GFELAVSRPRVVFRMVDG----------- 399 E + + G LH+ I+ E + RE +L + P VV+ ++ G Sbjct: 329 EVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVIMGDGSLIKVDNPS 388 Query: 400 -------VRE--EPYENLTVDVEETHQGGVMEELGRRRGDLQNMEPDGKGRVRLEYHIP- 449 + E EP + + + + + G V+ +RG +M G+ +V+L Y +P Sbjct: 389 KMPEPSKIEEIREPIVTVNLYMPQEYVGSVITLCIAKRGIQMDMSYHGR-QVKLVYEMPM 447 Query: 450 ARGLIGFQGEFMTLTRGTGLMSHVFDDYKPVDTSKGELAGRRNGVLISQDDGAAVAYALW 509 A ++ F + + +RG M + F +Y+ D K ++ V D A+ Sbjct: 448 AEIVLDFFDKLKSTSRGYASMDYEFKEYRASDVVKVDMLINSEKV-----DALAIIVHRS 502 Query: 510 KLQDRGRMFVS 520 Q RGR S Sbjct: 503 NSQYRGRQVAS 513 >gi|134094178|ref|YP_001099253.1| peptide chain release factor 3 [Herminiimonas arsenicoxydans] Length = 564 Score = 89.7 bits (221), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 50/133 (37%), Positives = 74/133 (55%), Gaps = 6/133 (4%) Query: 9 RNIAIIAHVDHGKTTLVDQLL------RQSGTFRDNQQVDNRVMDSNDIEKERGITILSK 62 R II+H D GKTTL ++LL + +GT + + + D DIEK+RGI++ S Sbjct: 44 RTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKGRKSARHATSDWMDIEKQRGISVASS 103 Query: 63 NCAVEYKGTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALAL 122 EY+ +N++DTPGH DF + RVL+ VDS L+++DA +G QT + Sbjct: 104 VMQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVETQTIKLLNVCRMR 163 Query: 123 GLKPIVVLNKIDR 135 I +NK+DR Sbjct: 164 NTPIITFMNKLDR 176 >gi|134096324|ref|YP_001101399.1| elongation factor G [Herminiimonas arsenicoxydans] Length = 702 Score = 89.4 bits (220), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 54/143 (37%), Positives = 76/143 (53%), Gaps = 9/143 (6%) Query: 5 IRAIRNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQVD--NRVMDSNDIEKERGITILSK 62 I RNI I AH+D GKTT +++L +G + + D + E+ERGITI S Sbjct: 7 IERYRNIGISAHIDAGKTTTTERVLFYTGVNHKLGETHEGSATTDWMEQEQERGITITSA 66 Query: 63 NCAVEYKGT-------HINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFV 115 +KG HINI+DTPGH DF EVER + ++D ++ A G PQ+ V Sbjct: 67 AVTCFWKGMANNLPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETV 126 Query: 116 TRKALALGLKPIVVLNKIDRPGA 138 R+A + + +NK+DR GA Sbjct: 127 WRQANKYKVPRLAFVNKMDRTGA 149 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 58/232 (25%), Positives = 103/232 (44%), Gaps = 39/232 (16%) Query: 186 MEPLFDAILKYVPARKDDPDAP---------------------LQLQITSLEYSSYVGKI 224 ++ + DA++ Y+P+ D P P L +I + +VG++ Sbjct: 275 VQAMLDAVIDYLPSPIDIPPVPGLNESDEPIVRKAEDGEKFSALAFKIAT---DPFVGQL 331 Query: 225 GVGRILAGRIKGGQDVVWMNGPEDKPTKARINQVLTFKGLERVLVDEALAGDIVLINGIE 284 R +G + G V +N ++K K RI +++ + +R + E AGDI + G++ Sbjct: 332 CFIRCYSGTLNSGDTV--LNSVKEK--KERIGRIVQMQANQREEIKEMRAGDIAAVVGLK 387 Query: 285 EIGIGSTICAPDAPNGLPMLKVDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELK 344 + G T+C A L + EP ++ T + G + +RL E Sbjct: 388 DTTTGDTLCDDKAFVVLERMVFPEPVISQAVEPKTKADQEKMGLAL------NRLAAE-- 439 Query: 345 SNMALRVVQAENDDSTYEVSGRGELHLTILIENMRRE-GFELAVSRPRVVFR 395 + + RV E T ++G GELHL I+++ M+RE E V +P+V +R Sbjct: 440 -DPSFRVRTDEESGQTI-IAGMGELHLDIIVDRMKREFNVEATVGKPQVAYR 489 >gi|134096335|ref|YP_001101410.1| elongation factor Tu [Herminiimonas arsenicoxydans] Length = 396 Score = 82.0 bits (201), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 90/286 (31%), Positives = 125/286 (43%), Gaps = 39/286 (13%) Query: 10 NIAIIAHVDHGKTTLVDQLL-----RQSGTFRDNQQVDNRVMDSNDIEKERGITILSKNC 64 N+ I HVDHGKTTL + + G + Q+DN EK RGITI + + Sbjct: 14 NVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKGYDQIDNAPE-----EKARGITINTSHV 68 Query: 65 AVEYKGTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGL 124 E H VD PGHAD+ + + +D +L+ A +GPMPQTR A +G+ Sbjct: 69 EYETTTRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGV 128 Query: 125 KPIVV-LNKIDRPGARAEWAINATFELFDKLGATE-EQLDFPVIYASGLNGYASLDPETR 182 I+V LNK D AE E+ + L E D P+I G A L Sbjct: 129 PYIIVFLNKCDMV-DDAELLELVEMEVRELLSKYEFPGDDLPII-----KGSAKL---AL 179 Query: 183 EGNMEPLFD--------AILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRI 234 EG+ PL + A+ Y+P + D L + + S G + GRI G + Sbjct: 180 EGDQGPLGEAAILALADALDSYIPTPERAVDGAFLLPVEDVFSISGRGTVVTGRIERGIV 239 Query: 235 KGGQDVVWMNGPEDKPTKARINQVLTFKGLE--RVLVDEALAGDIV 278 K G+ + + R QV T G+E R L+D+ AGD V Sbjct: 240 KVGESLEIVG--------IRDTQVTTCTGVEMFRKLLDQGQAGDNV 277 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.317 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,139,267 Number of extensions: 50516 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 8 Length of query: 613 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 526 Effective length of database: 713,682 Effective search space: 375396732 Effective search space used: 375396732 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)