BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3504.p (589 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093980|ref|YP_001099055.1| dihydroxy-acid dehydratase [Her... 280 2e-76 gi|134094317|ref|YP_001099392.1| phosphogluconate dehydratase [H... 174 1e-44 >gi|134093980|ref|YP_001099055.1| dihydroxy-acid dehydratase [Herminiimonas arsenicoxydans] Length = 557 Score = 280 bits (715), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 200/546 (36%), Positives = 289/546 (52%), Gaps = 24/546 (4%) Query: 31 HRSTLAADGWTREDLMEKPIVGILNTWSDINPCNMNLRMLAQKVREGILEAGGIP--FEI 88 +RS A G+ + D +KP+VGI N S I PCN L+ LA + I +AGG P F Sbjct: 19 NRSMYYAMGYEKADF-DKPMVGIANGHSTITPCNSGLQKLADIAIKTIKDAGGNPQVFGT 77 Query: 89 PL----MSISENLIKPSSFPFRNMLAMDTEECIRGYPLDAVVLMGGCDKTQPGLLMGAAS 144 P MS+ +K S R ++A E + G +D V+++GGCDK PG ++ Sbjct: 78 PTISDGMSMGTEGMK-YSLISREVIADCIETAVNGQWMDGVLVIGGCDKNMPGGMIAMLR 136 Query: 145 ANVPAIALNSGPGTPRSAIGGPLATANGVWRFVDQLRAGCLSTESFENFERQTMGSPGHC 204 NVP+I + G P G L T + V + AG +S E F+ ER S G C Sbjct: 137 TNVPSIYVYGGTIKPGHWKGKDL-TIVSAFEAVGEFTAGRMSQEDFDGIERNACPSSGSC 195 Query: 205 SEMGTASTMSSISEALGLALPGSSLIPSSDGRRSQIAKAVGRCSVALALSGDLAPANILD 264 M TA+TMSS EALGLALP SS + + D + A R + A+ DL P +I+ Sbjct: 196 GGMYTANTMSSSFEALGLALPYSSTMANPDDEKVGSAAESARVLIE-AIKKDLKPRDIVT 254 Query: 265 ARAFDNAITLLCAVGGSTNAIIHLLALAGRVGVPLTLDRFDEIARRVPLISNVQPAGEHL 324 +A +NA+ ++ A GGSTNA++H LA+A + T+D F+ + ++VP+I +++P+G+++ Sbjct: 255 RKAIENAVAVIMATGGSTNAVLHFLAIAHAAEIDWTIDDFERMRKKVPVICDLKPSGKYV 314 Query: 325 TERLMNAGGVPALLHVL--RPLLHLDARTVDGRTIGE-IADQAEV--SDTDVIRQLDTPV 379 L AGG+P ++ VL LLH D T+ G+T+ E +A V +D DVI +D + Sbjct: 315 ATDLHKAGGIPQVMKVLLDAGLLHGDCMTITGQTVAEALAHIPSVPSADQDVIHTIDKAL 374 Query: 380 KPGPSLVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLADTIDDPTMNIDANS 439 L ++KGNL+P G V K + G A VF+D + D I I A Sbjct: 375 YAQGHLAILKGNLSPEGCVAKITGLKNPVIT--GPARVFDDEYSAMDAI--MANQIKAGD 430 Query: 440 VLILRNVGPVGA-AMPEWGMLPLPRRLMEHGVKDML-RISDARMSGTAYGTSILHVSPEA 497 VL++R +GP G MPE ML L+ G+ + + I+D R SG +G + HVSPEA Sbjct: 431 VLVMRYLGPKGGPGMPE--MLAPTSALVGQGLGESVGLITDGRFSGGTWGMVVGHVSPEA 488 Query: 498 AVGGPLSLVRTGDIVELDVSERRLELIVSEEILAQRRKETPQHAPSPHVRGYRQLYGREV 557 VGG + LV GD V +D + ++L VSEE +A+RR Q AP + RG Y Sbjct: 489 FVGGTIGLVEEGDSVTIDARQLLIQLNVSEEEIARRRANWKQPAPR-YTRGVLAKYAALA 547 Query: 558 EQAHLG 563 A G Sbjct: 548 STASKG 553 >gi|134094317|ref|YP_001099392.1| phosphogluconate dehydratase [Herminiimonas arsenicoxydans] Length = 637 Score = 174 bits (440), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 154/515 (29%), Positives = 234/515 (45%), Gaps = 41/515 (7%) Query: 47 EKPIVGILNTWSDINPCNMNLRMLAQKVREGILEAGGIP-FE--IPLMSISENLIKPS-- 101 + P V I++ ++D+ + + +++ + AGG+ F +P M +P Sbjct: 66 KNPSVAIISAYNDMLSAHQPYERFPRIIKDAVRAAGGVAQFAGGVPAMCDGITQGQPGME 125 Query: 102 -SFPFRNMLAMDTEECIRGYPLDAVVLMGGCDKTQPGLLMGAAS-ANVPAIALNSGPGTP 159 S R+ +AM T + DA V +G CDK PGLL+GA ++PA+ + GP T Sbjct: 126 LSLFSRDTIAMSTAIALSHNMFDAAVFLGICDKIVPGLLIGALHFGHLPAVFIPGGPMT- 184 Query: 160 RSAIGGPLATANGVWRFVDQLRA-GCLSTESFENFERQTMGSPGHCSEMGTASTMSSISE 218 +N + QL A G + E+ E ++ G C+ GTA++ + E Sbjct: 185 -------TGMSNSEKSRIRQLYAQGKIDREALLEAESKSYHDAGTCTFYGTANSNQMLME 237 Query: 219 ALGLALPGSSLI-PSSDGRRSQIAKAVGRCSVALALSGDLAPA-NILDARAFDNAITLLC 276 +GL LPGS+ + P++ R + +A R A P ++D + NAI L Sbjct: 238 VMGLHLPGSAFVTPNTPLRDALTVRAAQRAVEISAQGQTFTPVGRLVDEKTIVNAIVALL 297 Query: 277 AVGGSTNAIIHLLALAGRVGVPLTLDRFDEIARRVPLISNVQPAGEHLTERLMNAGGVPA 336 GGSTN IHL+A+A G+ + + FD+++ VPL++ + P GE AGG Sbjct: 298 TTGGSTNHTIHLIAIAKAAGIVIDWNDFDQLSAVVPLLTRIYPNGEADVNHFHAAGGTGF 357 Query: 337 LLHVLRP--LLHLDARTVDGRTIGEI-------ADQAE-------VSDTDVIRQLDTPVK 380 ++ L LLH D T+ G + DQA +D V+R D P Sbjct: 358 VIRELLDAGLLHDDVTTILGEGLRAHCAEPFLDGDQAVWKPAPHISADESVLRPADRPFA 417 Query: 381 PGPSLVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLADTIDDPTMNIDANSV 440 P L ++ GNL AVIK A P + A+VF+ + D +N D V Sbjct: 418 PDGGLRLLTGNLGR--AVIKISAVKPEYHRVEAPAIVFKSQEEFMHAYQDGKLNQD--FV 473 Query: 441 LILRNVGPVGAAMPEWGML-PLPRRLMEHGVKDMLRISDARMSGTAYGT-SILHVSPEAA 498 ++R GP MPE L P L + G L ++D RMSG + + +HVSPE Sbjct: 474 AVVRFQGPKANGMPELHALTPALGSLQDAGFHVAL-VTDGRMSGASGKVPAAIHVSPEVL 532 Query: 499 VGGPLSLVRTGDIVELDVSERRLELIVSEEILAQR 533 GGPL +R GDI+ LD L+ +V QR Sbjct: 533 AGGPLGKIRDGDIILLDAEAGILQALVDAAEWQQR 567 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,147,611 Number of extensions: 48745 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 2 Length of query: 589 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 502 Effective length of database: 713,682 Effective search space: 358268364 Effective search space used: 358268364 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)