BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3384.p (567 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094267|ref|YP_001099342.1| 2-isopropylmalate synthase [Her... 825 0.0 gi|134094470|ref|YP_001099545.1| 2-isopropylmalate synthase [Her... 166 3e-42 >gi|134094267|ref|YP_001099342.1| 2-isopropylmalate synthase [Herminiimonas arsenicoxydans] Length = 519 Score = 825 bits (2131), Expect = 0.0, Method: Compositional matrix adjust. Identities = 396/519 (76%), Positives = 454/519 (87%), Gaps = 1/519 (0%) Query: 35 MSTDLRDGNQALIEPMNAERKLRFFELLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRI 94 MSTDLRDGNQ+LIEPMN ++KLRFF++L+K GLKEIEV FPSASQTDFDFVR+LI E RI Sbjct: 1 MSTDLRDGNQSLIEPMNHDKKLRFFDMLVKIGLKEIEVAFPSASQTDFDFVRRLIEEKRI 60 Query: 95 PDDVTIIVLTQSRDELIRRTVESLEGAKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVT 154 PDDVTI VLT +R++LIRRTVES+ GA++AI+HLYN+ APAFR+IVFNMSR+E+K +AV Sbjct: 61 PDDVTIEVLTPAREDLIRRTVESVVGARRAIIHLYNATAPAFRRIVFNMSRDEVKALAVA 120 Query: 155 GTRLVKELTDARPGTEWRFEYSPESFSTTELDFSKEICDAVCETWGATPERKVILNLPST 214 G +LV+ELTDA P TEW F+YSPE+FS TEL+FSKEICDAV TW TPERK+I NLP T Sbjct: 121 GAKLVRELTDAHPETEWVFQYSPEAFSMTELEFSKEICDAVSATWQPTPERKMIFNLPGT 180 Query: 215 VECATPNVYADQIEWMCRNLKDRASTIISVHPHNDRGTAVASAELAVMAGADRVEGCLFG 274 VE +TPNVYADQ+EWM RNL R S +ISVHPHNDRG AVA+AELAVMAGADRVEGCLFG Sbjct: 181 VEASTPNVYADQVEWMHRNLARRDSILISVHPHNDRGCAVAAAELAVMAGADRVEGCLFG 240 Query: 275 NGERTGNVDLVTLALNLYTQGVNPGLDFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTA 334 NGERTGNVDLVTLALNLY+QG++PGLDFSDID VRQ VEECNQIPVHPRHPYVGDLV+TA Sbjct: 241 NGERTGNVDLVTLALNLYSQGISPGLDFSDIDAVRQCVEECNQIPVHPRHPYVGDLVYTA 300 Query: 335 FSGSHQDAIKKGFAKQQPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQE 394 FSGSHQDAIKKGFA+QQPDAIWEVPY+P+DPADLGRSYDAVIRVNSQSGKGGM YLLEQE Sbjct: 301 FSGSHQDAIKKGFAQQQPDAIWEVPYMPVDPADLGRSYDAVIRVNSQSGKGGMTYLLEQE 360 Query: 395 YGLALPRRLQIEFSRAIQREADATGKEIAAADIHAIFQREYLERTEPYVYRAHRMSEDSS 454 YGL LPRRLQIEFSRA+QR D TG E+AA DIH IF EYL PY YR+H + E Sbjct: 361 YGLVLPRRLQIEFSRAVQRITDETGGEVAANDIHTIFNNEYLNAVTPYQYRSHHIDESQG 420 Query: 455 KAESINIEVDIVRNGQPVTVRGSGNGPIDAFVQALGLDIKLMDFHEHAIGAGADAKAASY 514 AE + I V++ ++G+PVT++G+GNGPIDAF+ ALG+DI+LMDFHEHAIGAGA+A AASY Sbjct: 421 NAE-VRIRVELEKDGKPVTLQGAGNGPIDAFINALGMDIRLMDFHEHAIGAGANANAASY 479 Query: 515 IELRLNEAPTGFGVGIDANIVTASFKAVLSAVNRQIAIS 553 +ELR+N T FGVGID NIVTAS +AVLSAVNR IA++ Sbjct: 480 VELRVNNGSTRFGVGIDGNIVTASLRAVLSAVNRDIALA 518 >gi|134094470|ref|YP_001099545.1| 2-isopropylmalate synthase [Herminiimonas arsenicoxydans] Length = 515 Score = 166 bits (419), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 149/532 (28%), Positives = 243/532 (45%), Gaps = 41/532 (7%) Query: 27 VISTPPIWMSTDLRDGNQALIEPMNAERKLRFFELLLKTGLKEIEVGFPSASQTDFDFVR 86 V S I T LRDG Q+ M + K+R L + + IE GF +ASQ DF+ + Sbjct: 3 VDSNKLIIFDTTLRDGEQSPGASMTRDEKIRIARQLERLKVDIIEAGFAAASQGDFESIH 62 Query: 87 KLIVENRIPDDVTIIVLTQSRDELIRRTVESLEGAKKAIVHLYNSVAPAFRKIVFNMSRE 146 + + I D T+ L+++ D+ I R E+L+ A + +H + + +P + M+ + Sbjct: 63 AI---SGIIKDATVCSLSRANDKDIARAAEALKPAARKRIHTFLATSPLHMEKKLRMTPD 119 Query: 147 EIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFSTTELDFSKEICDAVCETWGATPERK 206 ++ A R ++ TD E+SPE S +++DF + +AV + GAT Sbjct: 120 QVFEQAKIAVRYARQFTD-------DVEFSPEDGSRSDMDFLCRVIEAVIKE-GATT--- 168 Query: 207 VILNLPSTVECATPNVYADQIEWMCRNLKDRASTIISVHPHNDRGTAVASAELAVM-AGA 265 +N TV A P +Y + + + + + + SVH HND G VA++ VM GA Sbjct: 169 --INFADTVGYAVPELYGNMLRTLRERIPNSDKAVWSVHCHNDLGMGVANSLAGVMIGGA 226 Query: 266 DRVEGCLFGNGERTGNVDLVTLALNLYTQ----GVNPGLDFSDIDVVRQVVEECNQIPVH 321 +VE + G GER GN L + + + T+ + G+D + I ++V + V Sbjct: 227 RQVECTINGLGERAGNTALEEIVMAVRTRKDYFNLEIGVDATQIVPTSKLVSQITGFAVQ 286 Query: 322 PRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYLPIDPADLGRSYDAVIRVNSQ 381 P VG F SG HQD + K Y + D+G S + ++ + Sbjct: 287 PNKSVVGANAFAHASGIHQDGVLKA----------RDTYEIMRAEDVGWSANKIV-LGKL 335 Query: 382 SGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKEIAAADIHAIFQREYLERTEP 441 SG+ LE E G+ + + + +E EI DI A+ E Sbjct: 336 SGRNAFKQRLE-ELGITMESAADTDAAFIRFKELADRKSEIFDEDIMALVSDEQHSHEHE 394 Query: 442 YVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNGPIDAFVQALGLDI----KLMD 497 Y Y +++ SS A V G+ +G GNGP+DA V A+ + +L+ Sbjct: 395 Y-YNFVSLTQQSSSASKPVASVVFSMGGKEEICKGEGNGPVDAIVNAIESKVASGAELLL 453 Query: 498 FHEHAIGAGADAKAASYIELRLNEAPTGF-GVGIDANIVTASFKAVLSAVNR 548 F +AI G +++ + +RL++A GVG D +IV AS KA +SA+N+ Sbjct: 454 FSVNAITTGTESQGE--VTMRLSKAGRIVNGVGADLDIVVASAKAYISALNK 503 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,025,501 Number of extensions: 41632 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 2 Length of query: 567 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 480 Effective length of database: 713,682 Effective search space: 342567360 Effective search space used: 342567360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)