BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3303.p (293 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096009|ref|YP_001101084.1| spermidine/putrescine ABC trans... 55 4e-09 >gi|134096009|ref|YP_001101084.1| spermidine/putrescine ABC transporter inner membrane protein [Herminiimonas arsenicoxydans] Length = 258 Score = 55.1 bits (131), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 54/223 (24%), Positives = 107/223 (47%), Gaps = 13/223 (5%) Query: 24 WVTIYIPLLIFLFVLLFPFYWMVITAFKPDNELLSQTGNPFWVVAPTLAHFKKLLFDTQY 83 W+T+ +L++ F L P +V+ +F D+ L N WV TL ++ L D + Sbjct: 9 WLTLA-AVLVYAF-LYVPLAIVVMYSFN-DSRL-----NAEWV-GFTLDWYRVLFADEEM 59 Query: 84 PAWLLNTVIVSVVSTFASLAASVFAAYAIERLRFQGSKQVGLGIFLAYLIPPSILFIPLA 143 N++I+++VS+ S A +A+ R + + + + + IP ++ + L Sbjct: 60 LRAAGNSLIIALVSSAVSTVLGTMAGFALHRYKI---RTLQILVLTPIAIPEILVGVSLL 116 Query: 144 AIVFKLGLFDTRWALILTYPTFLIPFCTWLLMGYFRSIPYELEECALIDGATRWEILVKI 203 L + +++L + F + F ++ + L E A GAT W+ ++ Sbjct: 117 IFFVMLNITLGMLSVMLAHIAFSLGFVAIVVRSRLSGMDESLTEAARDCGATPWQAFRRV 176 Query: 204 ILPLAVPGLISAGIFAFTLSWNEFIYALTFISSSEVKTVPVGI 246 LPL +PG+++ + AFTLS ++F+ F + + +T+P+ I Sbjct: 177 TLPLIMPGVVAGALMAFTLSIDDFVITF-FTAGANTQTLPLQI 218 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.329 0.143 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 468,593 Number of extensions: 16683 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 3 Length of query: 293 Length of database: 1,000,347 Length adjustment: 81 Effective length of query: 212 Effective length of database: 733,452 Effective search space: 155491824 Effective search space used: 155491824 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)