BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3259.p (466 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094444|ref|YP_001099519.1| isopropylmalate isomerase large... 875 0.0 gi|134094018|ref|YP_001099093.1| isopropylmalate isomerase large... 436 e-123 gi|134094989|ref|YP_001100064.1| aconitate hydratase [Herminiimo... 89 4e-19 >gi|134094444|ref|YP_001099519.1| isopropylmalate isomerase large subunit [Herminiimonas arsenicoxydans] Length = 466 Score = 875 bits (2260), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/466 (89%), Positives = 438/466 (93%) Query: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 M +TLYDKLW+SHVV T DDGT +LYIDRHLLHEVTSPQAFEGLKLAGR+PWR SANLMV Sbjct: 1 MAQTLYDKLWQSHVVDTGDDGTTVLYIDRHLLHEVTSPQAFEGLKLAGRRPWRVSANLMV 60 Query: 61 ADHNVPTTDRAQGIADPISRLQVETLDGNAKEFSLTYFGMNDKRQGIVHVIGPEQGATLP 120 ADHNVPTTDR++GIADP SRLQVETLD NA E+ LTYF M DKRQGIVHVIGPEQGATLP Sbjct: 61 ADHNVPTTDRSEGIADPTSRLQVETLDHNAHEYGLTYFSMRDKRQGIVHVIGPEQGATLP 120 Query: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 GMTVVCGDSHTSTHGAF ALAHGIGTSEVEHVLATQTLLA+KSKAMLVQVDGALP GVTA Sbjct: 121 GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLAQKSKAMLVQVDGALPVGVTA 180 Query: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 KDIVLA+IGKIGTAGGTGYAIEFAGSTIRSLSMEGRMT+CNMAIEAGARAGMVA D+TT+ Sbjct: 181 KDIVLAIIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTICNMAIEAGARAGMVAVDETTI 240 Query: 241 EYLKGRPFSPSGPHWERAVSYWRTLHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMVVAV 300 Y+KGRP SP GPHW+RAV YWRTLHSD GAKFDLVVTLNAAE+KPQV+WGTSPEMVV+V Sbjct: 241 NYVKGRPLSPVGPHWDRAVEYWRTLHSDVGAKFDLVVTLNAAEVKPQVSWGTSPEMVVSV 300 Query: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 D RVPDPDKEKD TKRDGMEKAL YM LKPNTPI DIRIDKVFIGSCTNSRI+DLR AA Sbjct: 301 DGRVPDPDKEKDATKRDGMEKALVYMGLKPNTPITDIRIDKVFIGSCTNSRIQDLRAAAA 360 Query: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 VVRGK RASNVKLAMVVPGSGLVKEQAEREGLDKIF+DAGFEWREPGCSMCLAMNADRLE Sbjct: 361 VVRGKHRASNVKLAMVVPGSGLVKEQAEREGLDKIFKDAGFEWREPGCSMCLAMNADRLE 420 Query: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGI GHFVDVR+L Sbjct: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIEGHFVDVRAL 466 >gi|134094018|ref|YP_001099093.1| isopropylmalate isomerase large subunit [Herminiimonas arsenicoxydans] Length = 470 Score = 436 bits (1121), Expect = e-123, Method: Compositional matrix adjust. Identities = 234/469 (49%), Positives = 300/469 (63%), Gaps = 5/469 (1%) Query: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 M +TLY KL ++H V D +L+ D HLL+E TSPQAF GL G + N+ Sbjct: 1 MARTLYQKLVDTHTVAQLDRDNVLLFADLHLLNEYTSPQAFAGLDERGLRALIPGQNVAT 60 Query: 61 ADHNVPTT-DRAQGIADPISRLQVETLDGNAKEFSLTYFGMNDKRQGIVHVIGPEQGATL 119 H +PTT D + I D S LQ L N + F ND QGI HVI PE G Sbjct: 61 VSHIIPTTPDPLRIIRDTDSALQASNLKRNCDLHHIPLFDTNDALQGIEHVIAPEHGMIR 120 Query: 120 PGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVT 179 PGM V+CGDSHT+T+GA AL GIGT+EVEHVLATQTL+ R +K M ++VDG LP G T Sbjct: 121 PGMVVICGDSHTTTYGALGALGFGIGTTEVEHVLATQTLVYRLAKNMRIRVDGKLPNGTT 180 Query: 180 AKDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTT 239 AKD+VL +I +IG G G+ +EF+G+ I +LS+E R T+CNMA+EAGAR G++A D Sbjct: 181 AKDLVLMIISRIGAQGARGFVVEFSGTAIDALSVEARFTLCNMAVEAGARGGLIAPDQAA 240 Query: 240 LEYLKGR-PFSPSGPHWE-RAVSYWRTLHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMV 297 ++Y+ R P + P W A++ W+ L SD+ A FD+ AA++ P VTWGTSP+ Sbjct: 241 VDYVMQRCPDIAANPAWRVAALADWQALRSDEDAAFDVDHVFAAADVAPFVTWGTSPDQA 300 Query: 298 VAVDARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLRE 357 + + +P+ D+E D K + E+AL Y L+ +E + + VFIGSCTN RIEDLR Sbjct: 301 IPISGHIPECDQENDAEK-ESTERALRYTGLQVGMSLEGLSVKHVFIGSCTNGRIEDLRA 359 Query: 358 AAKVVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNAD 417 + + A+ V+ AMVVPGSG V+ QAE+EG+ AGFEWR+PGCSMCLAMN D Sbjct: 360 VIATIGDRKVAAGVR-AMVVPGSGAVRMQAEQEGIAAKLIAAGFEWRKPGCSMCLAMNDD 418 Query: 418 RLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 L G RCAST+NRNFEGRQG G THL+SPAMAAAA + G DVR L Sbjct: 419 VLASGTRCASTTNRNFEGRQGRGSITHLMSPAMAAAAAVTGTITDVRKL 467 >gi|134094989|ref|YP_001100064.1| aconitate hydratase [Herminiimonas arsenicoxydans] Length = 912 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 97/363 (26%), Positives = 152/363 (41%), Gaps = 28/363 (7%) Query: 123 TVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTAKD 182 T+V DSHT+ + G+G E E + Q + + V + G L GVTA D Sbjct: 209 TLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGQLREGVTATD 268 Query: 183 IVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEY 242 +VL + + G +EF G SLS+ R T+ NMA E GA G DD T++Y Sbjct: 269 LVLTITELLRKEKVVGKFVEFFGEGTESLSLTDRATIANMAPEYGATMGFFPVDDATIDY 328 Query: 243 LKG----RPFSPSGPHWERAVSYWRTLHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMVV 298 KG + + ++ +A + S + + VV L+ + P + P+ + Sbjct: 329 FKGTGRTKAEIDAFENYFKAQGLYGIPKSGE-IDYTHVVALDLGSVAPSLAGPKRPQDRI 387 Query: 299 AV-DARVPDPDKEKDPTKRDGMEKALAYM-ALKPNTPIEDIRIDKVFIG---SCTNSRIE 353 + + + D PT +G K + A N+ ++ V I SCTN+ Sbjct: 388 EIGNVKQNFADLFAKPTSENGFNKKAEDLDATYTNSDGVKVKNGDVLIAAITSCTNTSNP 447 Query: 354 DLREAAKVVRGKFRASNVKLA-----MVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGC 408 + A ++ K + +K++ + PGS +V E + GL GF GC Sbjct: 448 SVLLTAGLLAKKAVEAGLKVSPHIKTSLAPGSRVVTEYLQAAGLLPYLEKLGFGVTAYGC 507 Query: 409 SMCLAMNADRLEPGERCAS-----------TSNRNFEGRQGAGGRTH-LVSPAMAAAAGI 456 + C+ NA L P A + NRNFE R R++ L SP + A I Sbjct: 508 TTCIG-NAGDLTPAMNEAIVQNDIVASAVLSGNRNFEARIHPNIRSNFLASPPLVVAYAI 566 Query: 457 AGH 459 AG+ Sbjct: 567 AGN 569 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 864,848 Number of extensions: 34777 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 3 Length of query: 466 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 381 Effective length of database: 720,272 Effective search space: 274423632 Effective search space used: 274423632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)