BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3257.p (356 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094446|ref|YP_001099521.1| 3-isopropylmalate dehydrogenase... 623 e-180 gi|134096382|ref|YP_001101457.1| tartrate dehydrogenase [Hermini... 171 4e-44 gi|134096423|ref|YP_001101498.1| putative isocitrate dehydrogena... 153 1e-38 >gi|134094446|ref|YP_001099521.1| 3-isopropylmalate dehydrogenase [Herminiimonas arsenicoxydans] Length = 356 Score = 623 bits (1607), Expect = e-180, Method: Compositional matrix adjust. Identities = 301/356 (84%), Positives = 323/356 (90%) Query: 1 MKIAILPGDGIGPEIVDEAVRVLNALDEKFEMETAPVGGAGYEAHGHPLPDGTLKLAKEA 60 MKIAILPGDGIGPEI+++AV+V+NAL EKFE ET PVGGA Y GHPLP+ TLK+AKEA Sbjct: 1 MKIAILPGDGIGPEIMNQAVKVMNALGEKFETETVPVGGAAYAEQGHPLPESTLKVAKEA 60 Query: 61 DAILFGAVGDWKYDKLERALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA 120 DAILFGAVGD+KYD LER+LRPEQAILGLRKHL LFANFRPAI YPEL GASSLKPE+V+ Sbjct: 61 DAILFGAVGDFKYDTLERSLRPEQAILGLRKHLGLFANFRPAILYPELAGASSLKPEIVS 120 Query: 121 NLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK 180 LDI+IVREL GDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAA K Sbjct: 121 GLDIMIVRELTGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAAQK 180 Query: 181 RGKRLCSVDKANVLETFQFWKDIVTEVGKEYPDVELSHMYVDNAAMQLVKAPKNFDVIVT 240 R KRL SVDKANVLETFQFW+D+VT+V KEY DV+L HMYVDNAAMQLV+APK FDV+VT Sbjct: 181 RSKRLTSVDKANVLETFQFWRDVVTDVHKEYKDVKLDHMYVDNAAMQLVRAPKEFDVVVT 240 Query: 241 GNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATILSA 300 GNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGK IANPLA ILS Sbjct: 241 GNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKDIANPLAQILSL 300 Query: 301 AMMLRFSLNRAEQADRIENAVKKVLAQGLRTADIYEEGCTKVNTQQMGEAVVKALA 356 AMMLR+SL + EQADRIE AVK VL QGLRT DIYE G TKV+T QMG+AVVKALA Sbjct: 301 AMMLRYSLGKTEQADRIETAVKAVLKQGLRTVDIYEAGTTKVSTTQMGDAVVKALA 356 >gi|134096382|ref|YP_001101457.1| tartrate dehydrogenase [Herminiimonas arsenicoxydans] Length = 356 Score = 171 bits (434), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 128/371 (34%), Positives = 184/371 (49%), Gaps = 35/371 (9%) Query: 1 MKIAILPGDGIGPEIVDEAVRVLNALDEKF--EMETAPVGGAG---YEAHGHPLPDGTLK 55 IAI+PGDGIG E++ E +RVL+A ++F +E + A Y G +PD + Sbjct: 5 FNIAIIPGDGIGKEVIPEGLRVLDAAAKRFGIHLEYKHIEWASCDYYAQTGKMMPDDWKQ 64 Query: 56 LAKEADAILFGAVGDWKYDKLERALRPEQ-----AILGLRKHLQLFANFRPAICYPEL-T 109 + DAI FGAVG W A P+ ++L R+ + N RP + + Sbjct: 65 QLQGCDAIYFGAVG-WP------ATVPDHVSLWGSLLKFRREFDQYVNLRPVRLFEGVPC 117 Query: 110 GASSLKPELVANLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRR 169 ++ KP ++D +VRE Y M E + + F S R Sbjct: 118 PLANRKP---GDIDFYVVRENTEGEYTAIGGIMYEGTERELVLQQSAF-----SRRGTDR 169 Query: 170 IAHVAFQAAAKRGKRLCSV-DKAN-VLETFQFWKDIVTEVGKEYPDVELSHMYVDNAAMQ 227 I AF+ A R +R +V K+N + + +W E+ K YPD+ + ++D A + Sbjct: 170 ILKYAFELAQSRARRRLTVATKSNGIAISMPWWDSRADEMAKNYPDITVDKQHIDILAAR 229 Query: 228 LVKAPKNFDVIVTGNMFGDILSDAAAMLTGSIGMLPSASLDANN--KGLYEPSHGSAPDI 285 V P FDV+V N+FGDILSD TG++G+ PSA+L+ L+EP HGSAPDI Sbjct: 230 FVLQPDRFDVVVASNLFGDILSDLGPACTGTMGLAPSANLNPERLFPSLFEPVHGSAPDI 289 Query: 286 AGKGIANPLATILSAAMMLRFSLNRAEQADRIENAVKKVLAQGLRTADIYEEGCTKVNTQ 345 G+ IANP+A I S A+ML F RA D I A+K+VLA G RT D+ T Sbjct: 290 YGQNIANPIAMIWSGALMLDFLGQRAAH-DAILEAIKQVLAHGPRTPDL----GGNAGTA 344 Query: 346 QMGEAVVKALA 356 +G A+ A+A Sbjct: 345 DIGMAIAAAVA 355 >gi|134096423|ref|YP_001101498.1| putative isocitrate dehydrogenase [NAD] subunit alpha [Herminiimonas arsenicoxydans] Length = 348 Score = 153 bits (386), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 129/359 (35%), Positives = 181/359 (50%), Gaps = 28/359 (7%) Query: 1 MKIAILPGDGIGPEIVDEAVRVLNALDEKFEMETAPVGGAGYEAHGHPLPDGTLKLAKEA 60 + + ++PGDGIGPEIVD VRV +AL F ET G E G LP TL Sbjct: 10 IPVTLIPGDGIGPEIVDVVVRVFDALGNPFAWETQQAGVNALEKSGDLLPQVTLDSITRT 69 Query: 61 DAILFGAVGDWKYDKLERALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA 120 L G + + R + LR+ QL+AN RPA + G K Sbjct: 70 GLALKGPLST----PIGGGFRSVN--VRLRETFQLYANVRPARTI--VPGGRYEK----- 116 Query: 121 NLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK 180 +D+++VRE +Y G + D G +T S RRI+ AF A + Sbjct: 117 -IDLVLVRENLEGLYVGHEHYVPIGDDAHAVAMATGINTRAGS----RRISKFAFDYAVR 171 Query: 181 RG-KRLCSVDKANVLETFQ-FWKDIVTEVGKEYPD-VELSHMYVDNAAMQLVKAPKNFDV 237 K++ V KANVL+ + + +VG Y + VE VD AMQLV P FDV Sbjct: 172 NNRKKVTIVHKANVLKALTGLFLETAKQVGLNYANQVEFDDRIVDACAMQLVLNPWQFDV 231 Query: 238 IVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATI 297 IV+ N+FGDILSD A L G +GM P A++ ++E HGSAPDIAGKG+ANP A + Sbjct: 232 IVSTNLFGDILSDQIAGLVGGLGMAPGANI-GERTAIFEAVHGSAPDIAGKGMANPTALL 290 Query: 298 LSAAMMLRF-SLNRAEQADRIENAVKKVLAQGLRTADIYEEGCTKVNTQQMGEAVVKAL 355 L+AA+ML LN QA + +A+ VL+ + ++ G T V+T + G+ +++ + Sbjct: 291 LAAALMLEHKGLN--TQAGLLRDAIDSVLST--ESGRTFDLGGT-VSTSKYGDLLIERI 344 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 704,968 Number of extensions: 30544 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 3 Length of query: 356 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 273 Effective length of database: 726,862 Effective search space: 198433326 Effective search space used: 198433326 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)