BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3246.p (166 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094456|ref|YP_001099531.1| membrane protein required for c... 143 5e-36 >gi|134094456|ref|YP_001099531.1| membrane protein required for colicin V production [Herminiimonas arsenicoxydans] Length = 127 Score = 143 bits (360), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 71/127 (55%), Positives = 90/127 (70%) Query: 36 VALVVANTYGEALSAMLPDVVPGELTRLIVAFIALFIGTRLLVALVAKTLGELVKATGLG 95 +A VVAN YGE+L+ +LPD+VPG TRLIV FI LFIG R+L+ L+ L +VKA+GL Sbjct: 1 MAFVVANAYGESLAQLLPDMVPGNTTRLIVGFIVLFIGVRILMGLLTMALDAVVKASGLT 60 Query: 96 FLDRTLGSVFGVARGVLLVVAVALLCGTTEIPRQPFWTQARFSPWVVQAAQAVLPYLPGK 155 DR LG +FG+ARG+L+V+A LLCG T IP+Q FW +A FSP AA V P+LPG Sbjct: 61 LADRGLGGLFGLARGLLIVLAGVLLCGMTAIPQQGFWKEALFSPLAETAALTVKPFLPGD 120 Query: 156 LAEHVSF 162 A H+ F Sbjct: 121 FARHIKF 127 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.328 0.143 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 274,346 Number of extensions: 9112 Number of successful extensions: 51 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 51 Number of HSP's successfully gapped: 1 Length of query: 166 Length of database: 1,000,347 Length adjustment: 75 Effective length of query: 91 Effective length of database: 753,222 Effective search space: 68543202 Effective search space used: 68543202 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)