BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3172.p (385 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094652|ref|YP_001099727.1| MFS family transporter [Hermini... 348 2e-97 gi|134096093|ref|YP_001101168.1| transport protein [Herminiimona... 75 5e-15 gi|134095705|ref|YP_001100780.1| MFS family transporter [Hermini... 67 1e-12 gi|134094220|ref|YP_001099295.1| major facilitator superfamily m... 57 2e-09 gi|134093380|ref|YP_001098455.1| putative transporter [Herminiim... 55 5e-09 >gi|134094652|ref|YP_001099727.1| MFS family transporter [Herminiimonas arsenicoxydans] Length = 390 Score = 348 bits (894), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 193/365 (52%), Positives = 246/365 (67%) Query: 15 IGTTEFVIMGLLPDVAGDLKVSAPAAGMLVSGYALGVAAGAPILAILTAKWPRKQALVGL 74 IGTTEFVIMGLLPDVA L VS P+AG L+SGYALGVA GAPI+A+ TA PRK+AL+ L Sbjct: 15 IGTTEFVIMGLLPDVARSLSVSIPSAGWLISGYALGVAIGAPIMAVATASLPRKRALLLL 74 Query: 75 MCLFILGNVLCAMAPNYNFLMVARVVTAFCHAAFFGIGSVVAAELVPPQKRAQAVALMFT 134 M +FI+GNVLCA+A +YNFLMVAR++TA CH AFFGIGSVVAA LVP K+A AVALMFT Sbjct: 75 MGIFIVGNVLCAVATDYNFLMVARIITALCHGAFFGIGSVVAASLVPENKKASAVALMFT 134 Query: 135 GLTVANILGVPLGTAFGHVAGWRATFWAVTGIGALAATMLWTMLPRHLPMQGGNIFQEFR 194 GLT+AN+LGVPLGTA G VAGW+ F V G L+ LW ++P + ++ E Sbjct: 135 GLTLANVLGVPLGTALGQVAGWQMPFRVVAVFGVLSLIGLWRVMPGGQDEEKTDLRGEIV 194 Query: 195 SIIDTRVQLSLLTSTLASISMFSVITYIAYILLNVTGFSAQEESWVLLLFGAGITVGGLV 254 + + + +L T+ L S + F++ TY+A +L +VT S Q +W L L G G+TVG + Sbjct: 195 QLRNAGLWAALSTTMLFSAATFALFTYVAPLLEDVTHLSPQGITWTLFLIGLGLTVGNYI 254 Query: 255 GGKLADWKLMPVMCAVFLCIAIVLGLFSFTSHYKWPTLATMFAWGAFAFAVVPGAQIRVL 314 GG+LADW L + +FL +AIV + +TS P +F W +FA VPG Q+ V+ Sbjct: 255 GGRLADWSLGTALTGIFLVLAIVSSIVRWTSPSLIPVEINLFLWAMVSFAAVPGLQVNVM 314 Query: 315 DTARGAPNLASTLNQGAFNLGNASGAWIGGLAISAGLPLTDLPLIGSVFALLGLATVAFS 374 + APNLA+TLN GAFNLGNA GAW+GG ISAG L +PL V AL + + S Sbjct: 315 IFGKAAPNLAATLNIGAFNLGNALGAWVGGSVISAGFGLQAVPLAAGVLALTAVLAMRVS 374 Query: 375 ASLDK 379 L + Sbjct: 375 LRLAR 379 >gi|134096093|ref|YP_001101168.1| transport protein [Herminiimonas arsenicoxydans] Length = 385 Score = 75.5 bits (184), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 70/253 (27%), Positives = 125/253 (49%), Gaps = 18/253 (7%) Query: 21 VIMGLLPDVAGDLKVSAPAAGMLVSGYALGVAAGAPILAILTAKWPRKQALVGLMCLFIL 80 ++M L P + L + G+L+S Y AA + A ++ R++ ++ L LFI+ Sbjct: 2 IMMPLGPQLIRVLHIDTHQFGVLLSSYTFTAAASGLLAASYIDRFDRRKLILTLYALFII 61 Query: 81 GNVLCAMAPNYNFLMVARVVTAFCHAAFFGI-GSVV---AAELVPPQKRAQAVALMFTGL 136 + C +AP+Y L++AR + AF GI G++V A+++P ++R +A+ + T Sbjct: 62 ATLCCGLAPDYTTLLIARALA----GAFGGILGAMVQTMVADVIPFERRGKAMGTVMTAF 117 Query: 137 TVANILGVPLG----TAFGHVAGWRATFWAVTGIGA---LAATMLWTMLPRHLPMQG-GN 188 +++ + GVPLG ++F + GWRA F+ + + A L L L HL + GN Sbjct: 118 SISTVAGVPLGLLLASSFPAI-GWRAPFFFIVLLAAGFWLIGYKLLPSLTAHLHSKRVGN 176 Query: 189 IFQEFRSIIDTRVQLSLLT-STLASISMFSVITYIAYILLNVTGFSAQEESWVLLLFGAG 247 IFQ+ ++ L+ L ++ F+VI YIA + G + + L GA Sbjct: 177 IFQQIFTVAKEPSHLTAFAFIALLMMAGFTVIPYIALYMTTNVGLPESFITVIYLCGGAA 236 Query: 248 ITVGGLVGGKLAD 260 + G+++D Sbjct: 237 TFFTSQLIGRMSD 249 >gi|134095705|ref|YP_001100780.1| MFS family transporter [Herminiimonas arsenicoxydans] Length = 375 Score = 67.0 bits (162), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 47/151 (31%), Positives = 76/151 (50%) Query: 10 VAAFGIGTTEFVIMGLLPDVAGDLKVSAPAAGMLVSGYALGVAAGAPILAILTAKWPRKQ 69 +A+F + TE + GLLP +A L S G L++ YALG A LA T R+ Sbjct: 1 MASFIVILTEVLPAGLLPQIAASLNSSEALMGQLITVYALGSLVSAIPLAAATQGVDRRT 60 Query: 70 ALVGLMCLFILGNVLCAMAPNYNFLMVARVVTAFCHAAFFGIGSVVAAELVPPQKRAQAV 129 LV + F + N + A++ N + VAR + + + + AA +VP ++ +A+ Sbjct: 61 LLVIAIAGFGIVNTITAVSDNLIVIFVARFLGGVAAGLVWSMIAGYAARMVPENQKGRAI 120 Query: 130 ALMFTGLTVANILGVPLGTAFGHVAGWRATF 160 A+ G +A +G+P+G G+ GWR F Sbjct: 121 AIAMAGTPLALTMGIPIGAFLGNFVGWRIAF 151 >gi|134094220|ref|YP_001099295.1| major facilitator superfamily multidrug transporter; membrane protein [Herminiimonas arsenicoxydans] Length = 517 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 43/154 (27%), Positives = 72/154 (46%), Gaps = 4/154 (2%) Query: 26 LPDVAGDLKVSAPAAGMLVSGYALGVAAGAPILAILTAKWPRKQALVGLMCLFILGNVLC 85 +P ++GDL VS +++ +A+ A P+ LT ++ + + + + LF+L + LC Sbjct: 42 IPAISGDLGVSPQQGTWVITSFAVANAISVPLTGWLTQRFGQVRLFISSILLFVLASCLC 101 Query: 86 AMAPNYNFLMVARVVTAFCHAAFFGIGSVVAAELVPPQKRAQAVALMFTGLT--VANILG 143 +AP+ L+ ARV+ + + PP K A+A F G+T VA I+G Sbjct: 102 GLAPSIEILIAARVLQGAVAGPMIPLSQALLLSSYPPAKSGMALA--FWGMTTLVAPIMG 159 Query: 144 VPLGTAFGHVAGWRATFWAVTGIGALAATMLWTM 177 LG W F+ IG AA W++ Sbjct: 160 PLLGGWISDNYTWPWIFYINIPIGIFAAWATWSI 193 >gi|134093380|ref|YP_001098455.1| putative transporter [Herminiimonas arsenicoxydans] Length = 460 Score = 55.5 bits (132), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 56/205 (27%), Positives = 91/205 (44%), Gaps = 19/205 (9%) Query: 5 LFALAVAAFGIGTTEFVIMG-LLPDVAGDLKVSAPAAGMLVSGYALGVAAGAPILAILTA 63 LF + A+ + + IM +L + + +S AG+L S LG+ GA I +L Sbjct: 29 LFGIIATAWFFDSMDLGIMTFVLGSIKAEFGLSTAQAGLLASSSFLGMFLGAAIAGMLAD 88 Query: 64 KWPRKQALVGLMCLFILGNVLCAMAPNYNFLMVARVVTAFCHAAFFGIGSVVAAELVPPQ 123 K+ RK M + +G++ C A + + LM+ RV+ F F IG + +E+VP + Sbjct: 89 KYGRKPVFQWSMVFWGVGSLACGFAQDVDQLMIFRVILGFGMGMEFPIGLSMVSEIVPAK 148 Query: 124 KRAQAVALM-------FTGLTVANILGVPLGTAFGHVAGWRATFWAVTGIGALAATMLWT 176 R + VA++ F + L +PL GWR F A++ A + Sbjct: 149 SRGKYVAILEGFWPLGFIAAGILAYLTLPL-------IGWRGIFIALSVPAAFVFVIRRY 201 Query: 177 M--LPRHLPMQGGNIFQEFRSIIDT 199 + PR L G N +E +I T Sbjct: 202 VPESPRWLEEVGRN--EEAEKVIST 224 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.327 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 713,588 Number of extensions: 29364 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 7 Length of query: 385 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 302 Effective length of database: 726,862 Effective search space: 219512324 Effective search space used: 219512324 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)