BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3081.p (628 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Hermi... 997 0.0 gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 428 e-121 gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 87 2e-18 >gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 634 Score = 997 bits (2578), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/624 (75%), Positives = 535/624 (85%) Query: 3 YEDFYRRSIEHPDTYWAEEAQRIHWETPFTRTLDYSRAPFARWFIGGTTNLCYNAVDRHL 62 Y+ FYRRSI+ P T+WAE+AQRI W PFTRTLDY PF +WF+GG T+LCYNAVDRHL Sbjct: 8 YDAFYRRSIDDPHTFWAEQAQRIEWNKPFTRTLDYDHPPFTKWFVGGETSLCYNAVDRHL 67 Query: 63 AERPTQAALIAISTETNSERVYDFAELHREVQAMAATMLALGVQRGDRVLIYMPMIAEAV 122 A R Q ALIAISTET+SE+VY FA+LH EV AA M +LGV +GDRVLIYMPM+AEA Sbjct: 68 ATRADQPALIAISTETDSEKVYTFAQLHAEVNRCAAMMQSLGVSQGDRVLIYMPMVAEAA 127 Query: 123 FAMLACARIGAVHSVVFGGFASNSLASRIDDAQPCLIVSADAGSRGGKVIPYKGLLDEAI 182 FAMLACARIGA+HSVVFGGFASNSLA+RIDDA P LI+SADAGSRG V+PYK LLDEAI Sbjct: 128 FAMLACARIGAIHSVVFGGFASNSLATRIDDATPVLILSADAGSRGCHVMPYKSLLDEAI 187 Query: 183 NLAQHKPQRVLLVDRGLAEMARTPQRDVDYAQLRQQHLDQPVPVTWLESNDTSYVLYTSG 242 LA+HKP VL+V+RGLA M RD+DYA LR++H+D VPV WLESN SY+LYTSG Sbjct: 188 VLAEHKPTHVLMVNRGLAPMQTVAGRDIDYATLREKHMDAAVPVVWLESNAPSYILYTSG 247 Query: 243 TTGKPKGVQRDVGGYAVALASSMQHIFCGQPGQTYFCTSDIGWVVGHSYIVYGPLIAGMA 302 TTGKPKGV RDVGGYAVAL +SM IFCG+ G+T+FCTSDIGWVVGHSYI+YGPL+AGMA Sbjct: 248 TTGKPKGVVRDVGGYAVALTTSMDLIFCGKAGETFFCTSDIGWVVGHSYIIYGPLLAGMA 307 Query: 303 TVLYEGLPIRPDAGIWWSIVEKYKVSRMFSAPTAIRVLKKQPPECMERYDTSSLKALYLA 362 TVLYEGLPIRPDA IWWSIVEKYKV+RM SAPTAIRVLKKQP E ++++D SSLKALYLA Sbjct: 308 TVLYEGLPIRPDASIWWSIVEKYKVTRMSSAPTAIRVLKKQPLELLKKHDLSSLKALYLA 367 Query: 363 GEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGSPGVPMPGYRLA 422 GEPLD+TTS WISG L VPVIDNYWQTE+GWPIISIAKG+ DKPTR GSPG+P+ GY+ Sbjct: 368 GEPLDQTTSEWISGVLNVPVIDNYWQTETGWPIISIAKGVGDKPTRSGSPGLPLYGYKTK 427 Query: 423 ILDEATGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDARFVNTYWRSLPREVYSTFDWGIR 482 I++E +GE CAA EKGVV IEGPLPPGC+QT+YGDD RFV+TYWR+ RE YSTFDWGIR Sbjct: 428 IINEISGEECAANEKGVVIIEGPLPPGCLQTLYGDDQRFVSTYWRNFKREAYSTFDWGIR 487 Query: 483 DADGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAFAI 542 DADGYYFILGRTDDVINVAGHRLGTREIEESISSH NVSEVAVVGVE+ +KGQ A AF I Sbjct: 488 DADGYYFILGRTDDVINVAGHRLGTREIEESISSHGNVSEVAVVGVENPIKGQCAVAFVI 547 Query: 543 PKQADAIATPEQRKALEAEIMGVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQSIC 602 K A A+PE+ K LEAEIM VVDK++G++GRP+ V+FVS LPKTRSGKLLRR+IQ+IC Sbjct: 548 LKNAALAASPEEAKKLEAEIMKVVDKRLGSIGRPSHVYFVSALPKTRSGKLLRRSIQAIC 607 Query: 603 EGRDAGDLPTIEDPAALEQVRAAL 626 EGRD GDL IEDP+AL+Q+R AL Sbjct: 608 EGRDPGDLSVIEDPSALQQIRTAL 631 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 428 bits (1100), Expect = e-121, Method: Compositional matrix adjust. Identities = 245/626 (39%), Positives = 369/626 (58%), Gaps = 29/626 (4%) Query: 17 YWAEEAQR-IHWETPFTRTLDYSRAPFARWFIGGTTNLCYNAVDRHLAE-RPTQAALIAI 74 +WA AQ+ +HW PFT+ LD S APF +WF G N+ +N +DRHL + A+I Sbjct: 46 FWARLAQQNLHWTKPFTKKLDESNAPFYKWFEDGELNVSWNCLDRHLHNGNADKVAIIFE 105 Query: 75 STETNSERVYDFAELHREVQAMAATMLALGVQRGDRVLIYMPMIAEAVFAMLACARIGAV 134 + + N R+ + ELH V A + +L + +GDRV+IYMPM + + AM ACARIGA Sbjct: 106 ADDGNVTRI-TYKELHERVCKFANGLKSLSINKGDRVIIYMPMSIDGIAAMQACARIGAT 164 Query: 135 HSVVFGGFASNSLASRIDDAQPCLIVSADAGSRGGKVIPYKGLLDEAINLAQHKPQR-VL 193 HSVVFGGF++ SL RI DA +++AD RGGK +P K ++DEA+ + R V+ Sbjct: 165 HSVVFGGFSAKSLHERIIDAGAVAVLTADYQVRGGKNLPLKAIVDEALAMGGCDTIRNVV 224 Query: 194 LVDRGLAEMARTPQRDVDYAQLRQQHLDQ--PVPVTWLESNDTSYVLYTSGTTGKPKGVQ 251 + R + +RD+ +L +Q + P PV + ++LYTSG+TGKPKGVQ Sbjct: 225 VYKRTGGAVDFVERRDIWLHELVEQRTSECAPEPVG---AEHPLFILYTSGSTGKPKGVQ 281 Query: 252 RDVGGYAVALASSMQHIFCGQPGQTYFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPI 311 GY + +M+ +F +P Y+CT+DIGWV GH+Y+ YGPL G +++EG+P+ Sbjct: 282 HSSAGYLLWAMLTMKWVFDIKPNDVYWCTADIGWVTGHTYVTYGPLAVGATQIVFEGVPM 341 Query: 312 RPDAGIWWSIVEKYKVSRMFSAPTAIRVLKK----QPPECMERYDTSSLKALYLAGEPLD 367 P+AG +W +V+K+KVS ++APTAIR L K P +YD SSL+ L GEP++ Sbjct: 342 YPNAGRFWEMVQKHKVSIFYTAPTAIRSLIKAAEADPSTHPSKYDLSSLRLLGSVGEPIN 401 Query: 368 ETTSSWIS---GALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGSPGVPMPGYRLAIL 424 W G + P++D +WQTE+G +IS G + P GS +P+PG A++ Sbjct: 402 PEAWMWYYKNIGGERCPIVDTFWQTETGGHVISPLPGAT--PMVPGSCTLPLPGIIAAVV 459 Query: 425 DEATGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDARFVNTYW-RSLPREVYSTFDWGIRD 483 D+ G G+ G++ I+ P P ++ ++ D RF +Y+ L Y D IR+ Sbjct: 460 DDG-GHDLPNGKGGLLVIKRPW-PAMIRNIWNDPERFEKSYFPHELGGRTYLAGDGAIRN 517 Query: 484 AD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAFAI 542 + GY+ I GR DDV+N++GHR+GT EIE ++ ++P V+E AVVG D+ G+ AF + Sbjct: 518 EETGYFTITGRIDDVLNISGHRMGTAEIESALVANPMVAEAAVVGKPDETTGESICAFVV 577 Query: 543 PKQADAIATPE--QRKALEAEIMGVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 K+ PE + K + E+ V K+IG + +P + F LPKTRSGK++RR ++ Sbjct: 578 LKR----VRPEGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRV 633 Query: 601 ICEGRD-AGDLPTIEDPAALEQVRAA 625 + + D D+ T+E+PA LEQ++ A Sbjct: 634 LAKDEDITQDISTLENPAILEQLKQA 659 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 136/559 (24%), Positives = 224/559 (40%), Gaps = 90/559 (16%) Query: 81 ERVYDFAELHREVQAMAATMLALGVQRGDRVLIYMPMIAEAVFAMLACARIGAVHSVVFG 140 ++ +AE+ Q + A + G+Q+G RV + MP + + A+ A R G V V Sbjct: 46 DKFLTYAEVDTLSQKLGAWLQGTGLQKGARVALMMPNVLQYPVALAAVLRAGYVVVNVNP 105 Query: 141 GFASNSLASR-IDDAQPCLIVSADAGSRGGKVIPYKGLLDEAIN-----LAQHKPQRVLL 194 + L + +D +I+ + + K+I + + L K V Sbjct: 106 LYKPRELQHQLVDSGAEAIIILENFATTLEKIIVSTQIKHVVVATMGDMLGGLKGLLVNF 165 Query: 195 VDRGLAEMA---RTPQ----RDVDYAQLRQQHLDQPVPVTWLESNDTSYVLYTSGTTGKP 247 V R + +M PQ +DV AQ HL +P+ L D +++ YT GTTG Sbjct: 166 VVRHVKKMVPAFSLPQAVSFKDV-LAQAAHMHLQRPL----LGPQDIAFLQYTGGTTGTA 220 Query: 248 KG---VQRDVGGYAVALASSMQHIFCGQPGQ---TYFCTSDIGWVVGHSYIVYGPLIAGM 301 KG R++ + + + + +P + C + + + G+ Sbjct: 221 KGAMLTHRNIVANILQNEAWIWPVLRKEPPDKTLNFVCALPLYHIFALTACCMLGTRLGV 280 Query: 302 ATVLYEGLPIRPDAGIWWSIVEKYKVSRMFSAPTAIRVLKKQPPECMERYDTSSLKALYL 361 +L +P D + KYK++ + + T L P + D S LK Sbjct: 281 MNIL---IPNPRDIPSLVKELMKYKINLLPAVNTLYNGLLNNPD--FAKVDFSELKLCNG 335 Query: 362 AGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGISD-KPTRLGSP-------- 412 G + + V+++ W+ +G II G+S+ P +P Sbjct: 336 GGMAVQQ-------------VVNDRWRVVTGCSIIE-GYGLSETSPVATSNPVETTEFSG 381 Query: 413 --GVPMPGYRLAILDEATGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDARFVNTYWRSLP 470 G+P+P + ILD E A G G +AI GP Q + G YW + P Sbjct: 382 TIGLPVPSTEVMILDSNDNE-VALGLSGEIAIRGP------QVMAG--------YWNN-P 425 Query: 471 REV---------YSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVS 521 E + T D G+ DA GY I+ R D+I V+G + EIE ++ HP V Sbjct: 426 AETAQAMTADGFFKTGDIGVMDARGYTRIVDRKKDMILVSGFNVYPSEIENVVAGHPGVL 485 Query: 522 EVAVVGVEDKLKGQVAFAFAIPKQADAIATPEQRKALEAE-IMGVVDKQIGAVGRPARVF 580 E A +GV D+ G+ F + + AL AE +M +Q +P + Sbjct: 486 ECACIGVPDEHTGEAVKLFVVRRDP----------ALTAEALMAFCKEQFTGYKKPKMIE 535 Query: 581 FVSGLPKTRSGKLLRRAIQ 599 F + LPK+ GK+LRR ++ Sbjct: 536 FRTELPKSNVGKILRRELR 554 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,289,448 Number of extensions: 57008 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 3 Length of query: 628 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 541 Effective length of database: 713,682 Effective search space: 386101962 Effective search space used: 386101962 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)