BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3035.p (502 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde deh... 432 e-122 gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrog... 206 2e-54 gi|134095327|ref|YP_001100402.1| multifunctional: transcriptiona... 124 9e-30 >gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde dehydrogenase, oxidoreductase protein [Herminiimonas arsenicoxydans] Length = 506 Score = 432 bits (1110), Expect = e-122, Method: Compositional matrix adjust. Identities = 222/490 (45%), Positives = 312/490 (63%), Gaps = 5/490 (1%) Query: 1 MSQANIPHVPLYLNGEKVQSTAKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKDTFK 60 M + + + Y+NG + + V NPAT E+ A V A+ +V AVA AK Sbjct: 1 MKNSAVSTIEHYINGNLTNTVGGGQQPVFNPATGEISANVVLASDADVQTAVAAAKAAAP 60 Query: 61 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 W + +R RI+ KF+ L+ + LA ITREHGK DA+GEV RGLEVVE AC + Sbjct: 61 AWAEMAPLKRARILFKFKDLIERHHNDLAAAITREHGKVFSDAKGEVTRGLEVVEFACGV 120 Query: 121 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 L + ++N+ GG+D + L QPLGV AGIT FNFP M+P +M P+A+A GNTF+LKPS Sbjct: 121 PQLLKTQFSDNIGGGIDNWNLRQPLGVTAGITPFNFPFMVPMWMAPVALATGNTFILKPS 180 Query: 181 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR 240 E+DP+ SL + EL +QAGLP GV NV+ G + + HPDIKAVSF+GST + +IY+ Sbjct: 181 ERDPSPSLMVAELLHQAGLPDGVFNVLQGDKRAVDGLLQHPDIKAVSFVGSTPIAEYIYQ 240 Query: 241 ----RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVG 296 R R Q + GAKNH +V+PDA +QA++ L+GAA+G+AG+RCMA SV V VG Sbjct: 241 AGAARKGSYPLRVQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVG 300 Query: 297 QTRE-WIPEIVERSKTMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGR 355 + + ++ R K++KV G + A++GP+VS A K ++E I +G++ GA LL+DGR Sbjct: 301 SVADKLVAALIPRVKSLKVKNGMESDAEMGPVVSAAHKAKIEGYIEAGIKSGAKLLVDGR 360 Query: 356 NCKVAGSENGNFVGPTVFTGVKPDMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNG 415 V G NG F+G +F V P+M IY +EIFGP +C+V + A+ INA+ GNG Sbjct: 361 GLTVDGHSNGFFLGGCLFDNVTPEMTIYQEEIFGPVLCVVRVPDFSAALDLINAHEFGNG 420 Query: 416 TSIFTSSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFW 475 ++FTS G AR+F +++G VGINVPIPVP+A+ SF G + S GD G++ + F+ Sbjct: 421 VALFTSDGNTAREFSRRVEIGMVGINVPIPVPMAWHSFGGWKRSMFGDTHAYGEEGIKFY 480 Query: 476 TQTKTVTARW 485 T+ K++ RW Sbjct: 481 TRYKSIMQRW 490 >gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrogenase I, NADP-dependent [Herminiimonas arsenicoxydans] Length = 492 Score = 206 bits (525), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 137/444 (30%), Positives = 218/444 (49%), Gaps = 10/444 (2%) Query: 27 DVLNPATQEVVARVPFATKEEVDRAVANAKDTFKTWRNTSLAQRMRIMLKFQQLLRENIA 86 D+ NPA ++ P EE + A+ A+ F W + +R IM ++ L+ N+ Sbjct: 35 DISNPADGSIIGSAPNMGAEETETAIRAAQSAFPAWAAKTGKERAIIMRQWFDLIIANVD 94 Query: 87 PLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAENVAGGVDVYTLYQPLG 146 LA L+T E GK L ++ GEV+ G +E G++ + T+ QP+G Sbjct: 95 DLAILMTAEQGKPLIESRGEVIYGASFIEWFSEEAKRVSGDVMASTWNDKRTLTIKQPIG 154 Query: 147 VGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLVELANQAGLPPGVLNV 206 + A IT +NFP+ + AVA G T V+KP+EQ P S+L + ELA++AGLP GV+NV Sbjct: 155 ICAAITPWNFPIAMITRKVAPAVAAGCTIVIKPAEQTPLSALAIAELAHRAGLPAGVINV 214 Query: 207 VHGGPD----VANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQCMMGAKNHCIVL 262 + + V +C P ++ VSF GST VG + + + K+ +G IV Sbjct: 215 ITADAENSIVVGKTLCASPIVRHVSFTGSTPVGRILMEQCAPTVKKIALELGGHAPFIVF 274 Query: 263 PDAPKDQAINNLLGAAFGAAGQRCM-ANSVVVLVGQTREWIPEIVERSKTMKVGPGTDRK 321 DA D A+ L + + AGQ C+ N V ++ ++ + +KVG G ++ Sbjct: 275 DDADLDAAVTGALQSKYRNAGQTCVCTNRFYVHESIYDVFVQKLAAGAARIKVGNGFEQG 334 Query: 322 ADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKVAGSENGNFVGPTVFTGVKPDMD 381 GPL+ A +VE+ ++ V++GA ++ G+ + G+F PTV + + DM Sbjct: 335 VLQGPLIDDQAIRKVEQHVSDAVKKGASVMTGGKKHAL----GGHFYEPTVLSNITDDMS 390 Query: 382 IYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSIFTSSGYAARKFQNEIDVGQVGIN 441 I +E FGP IV+ +T DEAIA N G + + + +++ G VGIN Sbjct: 391 IMHEETFGPVAAIVKFKTEDEAIAAANNTDFGLASYFYARDISRVWRVAEKLEYGMVGIN 450 Query: 442 VPIPVPVAYFSFTGSRASKLGDLG 465 + + F G + S LG G Sbjct: 451 TGL-ISNEVAPFGGVKQSGLGREG 473 >gi|134095327|ref|YP_001100402.1| multifunctional: transcriptional repressor of proline utilization; proline dehydrogenase; pyrroline-5-carboxylate dehydrogenase [Herminiimonas arsenicoxydans] Length = 1224 Score = 124 bits (312), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 108/400 (27%), Positives = 187/400 (46%), Gaps = 33/400 (8%) Query: 30 NPATQ-EVVARVPFATKEEVDRAVANAKDTFKTWRNTSLAQRMRIMLKFQQLLRENIAPL 88 NPA +V+ A ++V+RA+A A + W+ T+ ++R I+L +L +++ Sbjct: 584 NPADHSDVLGTAVEANADDVERALALAIAGAEKWQKTAPSERAGILLNAAELFQQHAPEF 643 Query: 89 AELITREHGKTLPDAEGEVMRGLEVVEH-ACSITSLQLGELAENVAGGVDVYTLYQPLGV 147 L RE GKTLP+A GE+ ++ + + + IT+ Q+ Q LG+ Sbjct: 644 IALSVREAGKTLPNAIGELRETIDFLRYYSAQITTAQME----------------QALGL 687 Query: 148 GAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLVELANQAGLPPGVLNVV 207 A I+ +NFP+ + A+A GN + KP+EQ P + V+L +QAG+P VL + Sbjct: 688 VACISPWNFPLSIFTGQLSAALAAGNAVLAKPAEQTPLIAFRAVQLMHQAGVPEDVLLFL 747 Query: 208 HG-GPDVANMICDHPDIKAVSFIGSTHVGTHIYR---RASEAGKRAQCMM---GAKNHCI 260 G G + + V F GST V T I+R + S A +R ++ G +N I Sbjct: 748 PGRGQTTGAQLVADARVSGVVFTGSTQVATIIHRTLAQRSMAERRDIPLIAETGGQNAMI 807 Query: 261 VLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTREWIPEIVERS-KTMKVGPGTD 319 V A +Q + +++ +AF +AGQRC A V+ L E ++ + + ++ G Sbjct: 808 VDSSALCEQVVRDVIASAFDSAGQRCSALRVLCLQTDIAEKTLNMLHGAFEELQTGRPDQ 867 Query: 320 RKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKVAGS-ENGNFVGPTVFTGVKP 378 DVGP++ A++ + I EQ + + + + G FV P + + Sbjct: 868 LATDVGPVIDAEAQQHLRAYI----EQAGRRAKNMKQLALPSTGTQGTFVAPCIIEIDR- 922 Query: 379 DMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSI 418 M +E+FGP + ++ D + N G G ++ Sbjct: 923 -MSDLQREVFGPVLHVLRYRRDDLPQLVRDINATGFGLTL 961 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 956,675 Number of extensions: 39830 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 3 Length of query: 502 Length of database: 1,000,347 Length adjustment: 86 Effective length of query: 416 Effective length of database: 716,977 Effective search space: 298262432 Effective search space used: 298262432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)