BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2954.p (903 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094989|ref|YP_001100064.1| aconitate hydratase [Herminiimo... 1625 0.0 gi|134094444|ref|YP_001099519.1| isopropylmalate isomerase large... 98 2e-21 gi|134094018|ref|YP_001099093.1| isopropylmalate isomerase large... 86 7e-18 >gi|134094989|ref|YP_001100064.1| aconitate hydratase [Herminiimonas arsenicoxydans] Length = 912 Score = 1625 bits (4208), Expect = 0.0, Method: Compositional matrix adjust. Identities = 796/912 (87%), Positives = 851/912 (93%), Gaps = 9/912 (0%) Query: 1 MSRNTLNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVT 60 MSRNTLNTLKEF ISGSKKG FYSLPAL K LGVN+SRLP+SIRIVLESVLRNCDG+KVT Sbjct: 1 MSRNTLNTLKEFNISGSKKGTFYSLPALGKKLGVNLSRLPISIRIVLESVLRNCDGKKVT 60 Query: 61 EEHVKQLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEP 120 EEH+ QLANW A R DEIPFVVARVVLQDFTGVPLLADLAAMR VA+++GKNPKNIEP Sbjct: 61 EEHIHQLANWSPTATRTDEIPFVVARVVLQDFTGVPLLADLAAMRGVAARMGKNPKNIEP 120 Query: 121 LVPVDLVVDHSVQIDHFREKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGI 180 LVPVDLVVDHS+QIDHFR+K ALDLNMKLEF RNNERYQFMKWGMQAFDTFGVVPPGFGI Sbjct: 121 LVPVDLVVDHSIQIDHFRQKNALDLNMKLEFQRNNERYQFMKWGMQAFDTFGVVPPGFGI 180 Query: 181 VHQVNLEYLARGVHN-----KTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGML 235 VHQVNLEYLARGVH KT +YYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGML Sbjct: 181 VHQVNLEYLARGVHKRKNGGKTDIYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGML 240 Query: 236 GQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLT 295 GQPVYFLTPDVVGVNLTG LREGVTATDLVLTITELLR+ KVVGKFVEFFGEGTESLSLT Sbjct: 241 GQPVYFLTPDVVGVNLTGQLREGVTATDLVLTITELLRKEKVVGKFVEFFGEGTESLSLT 300 Query: 296 DRATIANMAPEYGATMGFFPVDDATIAYFKGTGRTKAEIDAFEAYFKAQGLYGVPKAGQI 355 DRATIANMAPEYGATMGFFPVDDATI YFKGTGRTKAEIDAFE YFKAQGLYG+PK+G+I Sbjct: 301 DRATIANMAPEYGATMGFFPVDDATIDYFKGTGRTKAEIDAFENYFKAQGLYGIPKSGEI 360 Query: 356 DYTQEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASY 415 DYT V+LDLG+VAPSLAGPKRPQDRIEIGNVK F+DLF KPT+ENGFNKKAEDL A+Y Sbjct: 361 DYTHVVALDLGSVAPSLAGPKRPQDRIEIGNVKQNFADLFAKPTSENGFNKKAEDLDATY 420 Query: 416 KNADGVDVHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSR 475 N+DGV V NGDVLIAAITSCTNTSNPSVLL AGLLAKKAVEAGLKV+PHIKTSLAPGSR Sbjct: 421 TNSDGVKVKNGDVLIAAITSCTNTSNPSVLLTAGLLAKKAVEAGLKVSPHIKTSLAPGSR 480 Query: 476 VVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNR 535 VVT+YL+AAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIV+ND+VA+AVLSGNR Sbjct: 481 VVTEYLQAAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVQNDIVASAVLSGNR 540 Query: 536 NFEARIHPNIRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGG----KPVYLGDIWPT 591 NFEARIHPNIR+NFLASPPLVVAYAIAGN+TRDLMTEPVG+GKG + VYLGDIWPT Sbjct: 541 NFEARIHPNIRSNFLASPPLVVAYAIAGNMTRDLMTEPVGRGKGADGKMRDVYLGDIWPT 600 Query: 592 SQEIEKLLKFAMNAKTFKENYADVKGAPGKLWEAIKGVAKGEVYNWPSSTYIAEPPFFEN 651 SQE+ KL+KFAMNAK FK+NYA VK APGKLWE IKGVA+G+VY+WPSSTYIAEPPFF + Sbjct: 601 SQEVSKLMKFAMNAKVFKDNYAQVKTAPGKLWEGIKGVAEGQVYDWPSSTYIAEPPFFND 660 Query: 652 FSEEPQAAATGISGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYG 711 F+ EP+AAATGI+ ARALGVFGDSITTDHISPAGSIK++SPAGK+L GVLKADFNSYG Sbjct: 661 FTMEPKAAATGITAARALGVFGDSITTDHISPAGSIKDTSPAGKYLTEKGVLKADFNSYG 720 Query: 712 SRRGNHEIMMRGTFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEG 771 SRRGNHEIMMRGTFANVRIKNLMIP K DGSRVEGGIT QP+GEEMSIYDAAM+YV+EG Sbjct: 721 SRRGNHEIMMRGTFANVRIKNLMIPIKPDGSRVEGGITRFQPTGEEMSIYDAAMRYVSEG 780 Query: 772 TPTMVFGGEEYGTGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDS 831 TPTMVF GEEYGTGSSRDWAAKGTQLLGVKAVIARS+ERIHRSNLVGMGVLPLQFLG+DS Sbjct: 781 TPTMVFAGEEYGTGSSRDWAAKGTQLLGVKAVIARSYERIHRSNLVGMGVLPLQFLGDDS 840 Query: 832 VQSLGITGEESFDLKGLEGEIKPQQEATLVIHRKNGQTQEVKLLLRIDTPIEVDYYKHGG 891 V +LGITG E+FDLKGLEG ++PQQE TLVIHR NGQ+Q+VK+LLRIDTPIEVDYYKHGG Sbjct: 841 VTTLGITGAETFDLKGLEGNLRPQQEVTLVIHRANGQSQDVKVLLRIDTPIEVDYYKHGG 900 Query: 892 ILPFVLRQLLAA 903 ILPFVLRQLLAA Sbjct: 901 ILPFVLRQLLAA 912 >gi|134094444|ref|YP_001099519.1| isopropylmalate isomerase large subunit [Herminiimonas arsenicoxydans] Length = 466 Score = 97.8 bits (242), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 114/391 (29%), Positives = 160/391 (40%), Gaps = 42/391 (10%) Query: 179 GIVHQVNLEYLARGVHNKTGVYYPD-TLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQ 237 GIVH + E G P T+V DSHT+ G + G+G E E + Q Sbjct: 106 GIVHVIGPEQ---------GATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQ 156 Query: 238 PVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDR 297 + + V + GAL GVTA D+VL I + G +EF G SLS+ R Sbjct: 157 TLLAQKSKAMLVQVDGALPVGVTAKDIVLAIIGKIGTAGGTGYAIEFAGSTIRSLSMEGR 216 Query: 298 ATIANMAPEYGATMGFFPVDDATIAYFKGTGRTKA--EIDAFEAYFKAQGLYGVPKAGQI 355 TI NMA E GA G VD+ TI Y KG + D Y++ L+ A Sbjct: 217 MTICNMAIEAGARAGMVAVDETTINYVKGRPLSPVGPHWDRAVEYWRT--LHSDVGA--- 271 Query: 356 DYTQEVSLDLGTVAPSLAGPKRPQDRIEI-GNVKSTFSDLFTKPTAENGFNKKAEDLGAS 414 + V+L+ V P ++ P+ + + G V D T +G K +G Sbjct: 272 KFDLVVTLNAAEVKPQVSWGTSPEMVVSVDGRVPD--PDKEKDATKRDGMEKALVYMGLK 329 Query: 415 YKNADGVDVHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGS 474 N D+ V I SCTN+ + AA ++ K + +K+A + PGS Sbjct: 330 -PNTPITDIRIDKVFIG---SCTNSRIQDLRAAAAVVRGKHRASNVKLA-----MVVPGS 380 Query: 475 RVVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDVVAAAVLSG 533 +V + E GL + GF GC+ C+ NA L P A + Sbjct: 381 GLVKEQAEREGLDKIFKDAGFEWREPGCSMCLAMNADRLEPGERCAS-----------TS 429 Query: 534 NRNFEARIHPNIRANFLASPPLVVAYAIAGN 564 NRNFE R R + L SP + A I G+ Sbjct: 430 NRNFEGRQGAGGRTH-LVSPAMAAAAGIEGH 459 >gi|134094018|ref|YP_001099093.1| isopropylmalate isomerase large subunit [Herminiimonas arsenicoxydans] Length = 470 Score = 86.3 bits (212), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 98/366 (26%), Positives = 144/366 (39%), Gaps = 32/366 (8%) Query: 205 LVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDL 264 ++ DSHTT +G +G+G+G E E + Q + + + + + G L G TA DL Sbjct: 125 VICGDSHTTTYGALGALGFGIGTTEVEHVLATQTLVYRLAKNMRIRVDGKLPNGTTAKDL 184 Query: 265 VLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATIANMAPEYGATMGFFPVDDATIAYF 324 VL I + G VEF G ++LS+ R T+ NMA E GA G D A + Y Sbjct: 185 VLMIISRIGAQGARGFVVEFSGTAIDALSVEARFTLCNMAVEAGARGGLIAPDQAAVDYV 244 Query: 325 KGTGRTKAEIDAFE--AYFKAQGLYGVPKAG-QIDYTQEVSLDLGTVAPSLAGPKRPQDR 381 A A+ A Q L A +D+ VAP + P Sbjct: 245 MQRCPDIAANPAWRVAALADWQALRSDEDAAFDVDHV----FAAADVAPFVTWGTSPDQA 300 Query: 382 IEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNAD-GVDVHNGDVLIAAITSCTNTS 440 I I S + EN K++ + Y G+ + V I SCTN Sbjct: 301 IPI-------SGHIPECDQENDAEKESTERALRYTGLQVGMSLEGLSVKHVFIGSCTNGR 353 Query: 441 NPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAAGLLPYLEKLGFGVTAY 500 + + + V AG ++ + PGS V E G+ L GF Sbjct: 354 IEDLRAVIATIGDRKVAAG------VRAMVVPGSGAVRMQAEQEGIAAKLIAAGFEWRKP 407 Query: 501 GCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEARIHPNIRANFLASPPLVVAYA 560 GC+ C+ AMN+ ++ + A+ + NRNFE R L SP + A A Sbjct: 408 GCSMCL--------AMNDDVLASGTRCAS--TTNRNFEGR-QGRGSITHLMSPAMAAAAA 456 Query: 561 IAGNVT 566 + G +T Sbjct: 457 VTGTIT 462 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.316 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,651,335 Number of extensions: 68552 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 3 Length of query: 903 Length of database: 1,000,347 Length adjustment: 90 Effective length of query: 813 Effective length of database: 703,797 Effective search space: 572186961 Effective search space used: 572186961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)