BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2908.p (762 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094841|ref|YP_001099916.1| murein polymerase, bifunctional... 99 1e-21 gi|134096281|ref|YP_001101356.1| murein polymerase, bifunctional... 84 2e-17 gi|134095915|ref|YP_001100990.1| monofunctional biosynthetic pep... 65 8e-12 >gi|134094841|ref|YP_001099916.1| murein polymerase, bifunctional murein transglycosylase and transpeptidase; penicillin-binding protein PBP1A; dimeric (Mca like) [Herminiimonas arsenicoxydans] Length = 837 Score = 98.6 bits (244), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 87/274 (31%), Positives = 129/274 (47%), Gaps = 26/274 (9%) Query: 346 AQRQAGSTLKPFLYELAIEKKWLTAASLLDDSPV---NLATSAG-LYIPQNYDKQYKGLV 401 A RQ GST+KPF+Y A+EK + + +L++D+P+ N G + P N + QY G V Sbjct: 488 AWRQPGSTIKPFVYSAALEKGF-SPGTLINDAPLAEDNPGEQGGKAWNPGNDNSQYDGPV 546 Query: 402 SLRTSLGSSLNIPAVRTLVMVTPERFFRRLQALGFNLRESGDYYGYSLALGSADVTLANL 461 ++RT L S N+ ++R L +TP L GF+ + D +LALG+ VT + Sbjct: 547 TMRTGLKKSKNLVSIRILRKITPPYAIEHLTHFGFDADKQPD--NLTLALGTGSVTPLQM 604 Query: 462 TNAYRVLANQG-RYSPLRTR---------LDQARTPA---RTTPVMDADAAFIIGDILSD 508 AY V AN G + +P + L +A P T +D AFI+ +L D Sbjct: 605 AGAYTVFANGGFKLAPYLIQKVVDVRGKVLLEANHPQAGDETARAIDPRNAFIMNTMLRD 664 Query: 509 RSARARTFGLENALSTRIWTAVKTGTSKDMRDNWCVGYSSRYTVGVWVGNASGAPMWDVS 568 R+ T + R A KTGT+ D D W GY W+G + + Sbjct: 665 -VVRSGTGYMAGQRLGRSDLAGKTGTTNDAMDGWFAGYGRDMVAVAWMGYDKPRTLGNRE 723 Query: 569 -GVTGAAPVWQALMQYLHERGREGGRDVRNAPPA 601 G T A P+W + Y+ R G +++NA PA Sbjct: 724 FGSTLALPIW---VDYMRTALR-GKAEIQNAVPA 753 Score = 96.7 bits (239), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 77/262 (29%), Positives = 128/262 (48%), Gaps = 35/262 (13%) Query: 2 RMTTCSVRPGALVSMSALVLALLIPTAASAVPSFSEVQRDFLPSEASLLDRNGELLQRIR 61 R T +R A+V++ A A L+ A + F V + LPS +L D ++ R+ Sbjct: 32 RPTDIKLRQAAIVAVIASFCAALLLVAYT----FLFVAPN-LPSIDALTDYRPKIPLRVY 86 Query: 62 I-------NMDERRLSWVKLEDVSPALRHALIASEDRRFYQHSGIDWSAVAASAWGNLWN 114 E R +V + ++ ++++A+IA ED FY+H G+D V + N+ Sbjct: 87 TADNVLIGEFGEERRDFVSIGEIPSSMKNAIIAIEDNNFYEHGGVDLLGVVRAGLANIVR 146 Query: 115 TKTRGASTITMQLAGLIYDNLRRKSGARSLTQKVSQAWIAQSLERSWRKDQILEAYLNLV 174 ++++GASTITMQ+A L RK ++ ++K+ + +A +E++ KDQILE Y+N + Sbjct: 147 SRSQGASTITMQVARTFL--LTRK---KTYSRKLQEVLLAYRIEQNLTKDQILELYMNQI 201 Query: 175 AFRGELVGVHALSRVMFRKHPSGLDQGEAAIAVALIRAPNA-----TPRRVAERACAILK 229 G + +R+ F K L EAA+ + +AP + P+R ER ILK Sbjct: 202 YLGQRAYGFGSAARIYFGKPIKDLSIAEAAMLAGIPKAPASANPVVNPKRATERQQYILK 261 Query: 230 -------------EEGAAEQCN 238 E+ AAEQ + Sbjct: 262 RMHELNFITQAQYEKAAAEQLH 283 >gi|134096281|ref|YP_001101356.1| murein polymerase, bifunctional murein transglycosylase and transpeptidase; penicillin-binding protein PBP1A; dimeric [Herminiimonas arsenicoxydans] Length = 683 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 71/254 (27%), Positives = 121/254 (47%), Gaps = 22/254 (8%) Query: 346 AQRQAGSTLKPFLYELAIEKKWLTAASLLDDSPVNL---ATSAGLYIPQNYDKQYKGLVS 402 A RQ GS+ KPF+Y ++EK L+ +++++D+P++ T + P+NYD +Y G ++ Sbjct: 375 AWRQPGSSFKPFIYSASLEKG-LSPSTIINDAPISFDAGQTGGQPWEPKNYDGKYDGPMT 433 Query: 403 LRTSLGSSLNIPAVRTLVMVTPERFFRRLQALGFNLRESGDYYGYSLALGSADVTLANLT 462 +R L S N+ ++R L + + + GF+ ++ Y +LALG+ VT + Sbjct: 434 MRRGLTKSKNMISIRILHKIGAKYGQEYITRFGFDADKNPPY--LTLALGAGAVTPLQMA 491 Query: 463 NAYRVLANQG-RYSP-LRTRLDQA--RTPARTTP---------VMDADAAFIIGDILSDR 509 + V AN G + +P L T++ A + + P V+DA AF++ +L D Sbjct: 492 AGFAVFANGGYKINPYLITKITDASGKPLSEVNPVKAGDEANRVIDARNAFLMDSMLKDV 551 Query: 510 SARARTFGLENALSTRIWTAVKTGTSKDMRDNWCVGYSSRYTVGVWVGNASGAPMWD-VS 568 ++ R A KTGT+ D D W GY W+G + + + Sbjct: 552 VTSGT--AVKAMALKRTDLAGKTGTTNDSFDAWFAGYHPEIVGIAWIGFDQPKNLGNRET 609 Query: 569 GVTGAAPVWQALMQ 582 G A P+W MQ Sbjct: 610 GGGLALPIWINYMQ 623 Score = 80.5 bits (197), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 47/154 (30%), Positives = 82/154 (53%), Gaps = 10/154 (6%) Query: 81 LRHALIASEDRRFYQHSGIDWSAVAASAWGNLWNTKTRGASTITMQLAGLIYDNLRRKSG 140 ++ A++A ED RF++H G+D+ + +A NL +GASTIT Q+A + S Sbjct: 1 MKKAVLAIEDDRFFEHGGVDYVGIVRAAVHNLSGGARQGASTITQQVARNFF-----LSS 55 Query: 141 ARSLTQKVSQAWIAQSLERSWRKDQILEAYLNLVAFRGELVGVHALSRVMFRKHPSGLDQ 200 ++L +K+ + +A +E++ KDQILE Y+N + G + +++ + K + Sbjct: 56 EQTLKRKIYEVLLAWKIEKNLSKDQILEVYMNQIYLGQRAYGFSSAAQIYYGKKLQNITI 115 Query: 201 GEAAIAVALIRAPNA-----TPRRVAERACAILK 229 EAA+ L +AP+A P+R R IL+ Sbjct: 116 AEAAMLAGLPKAPSAYNPVVNPKRARARQQYILQ 149 >gi|134095915|ref|YP_001100990.1| monofunctional biosynthetic peptidoglycan transglycosylase [Herminiimonas arsenicoxydans] Length = 231 Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 57/171 (33%), Positives = 82/171 (47%), Gaps = 11/171 (6%) Query: 71 WVKLEDVSPALRHALIASEDRRFYQHSGIDWSAVAASAWGNLWNTK-TRGASTITMQLAG 129 WV + +S L+ A+IASED F H GIDW A+ + N+ K G STIT QLA Sbjct: 64 WVPYKRISNNLKRAIIASEDSNFSGHEGIDWDALEKAYEKNVRKGKVVAGGSTITQQLAK 123 Query: 130 LIYDNLRRKSGARSLTQKVSQAWIAQSLERSWRKDQILEAYLNLVAFRGELVGVHALSRV 189 ++ SG RS +K + I LE K++I E YLN+V + + G A ++ Sbjct: 124 NLF-----LSGDRSYIRKGQEVVITYMLEYWMDKERIFEIYLNVVEWGVGVFGAEAAAQH 178 Query: 190 MFRKHPSGLDQGEAAIAVALIRAPNA--TPRRVA---ERACAILKEEGAAE 235 + + L +AA ++ P T R A R IL+ G+AE Sbjct: 179 YYGTSAAQLSAPQAARLAVMLPNPRFYDTHRGTAYLGRRTDLILRRMGSAE 229 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.132 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,419,644 Number of extensions: 58817 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 5 Length of query: 762 Length of database: 1,000,347 Length adjustment: 89 Effective length of query: 673 Effective length of database: 707,092 Effective search space: 475872916 Effective search space used: 475872916 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)