BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2801.p (374 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldeh... 229 2e-61 gi|134094301|ref|YP_001099376.1| putative glutathione-dependent ... 207 5e-55 gi|134095240|ref|YP_001100315.1| glutathione-independent formald... 61 1e-10 gi|134094820|ref|YP_001099895.1| putative alcool dehydrogenase w... 55 8e-09 >gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 369 Score = 229 bits (584), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 128/362 (35%), Positives = 200/362 (55%), Gaps = 5/362 (1%) Query: 17 FAVSQPLKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRPRPV-PMALGHEASAEV 75 F +PL+I ++D++PP GEVL+K+ G+CH+D ++G P + P+ LGHE + V Sbjct: 9 FGPGKPLEIVEIDVEPPRKGEVLIKITNTGVCHTDAFTLSGDDPEGLFPVVLGHEGAGIV 68 Query: 76 VQVGSGVTDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATNTVGQLLSGQRRLHDG 135 V+VG GVT ++ GD V+ ++ CG CL C G+ LC AT G + G R Sbjct: 69 VEVGEGVTSVKPGDHVIPLYTAECGECLFCKSGKTNLCVAVRATQGKGLMPDGTTRFSYN 128 Query: 136 SGHVHHHLGVSAFADHAVVSSRSCVKIEADIDPVEAALFGCAVLTGVGAAVNTAEVKAGT 195 ++H++G S F+++ VV+ S KI D +P L GC V TG+GA NTA+V+ G Sbjct: 129 GQPLYHYMGCSTFSEYTVVAEVSLAKINPDANPEHVCLLGCGVTTGIGAVHNTAKVQPGD 188 Query: 196 TAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALGATATVNARDPD--VVEK 253 + A+ GLGG+GL A+ GA + A +IA+D + +K E+AR GAT +N +D D + E Sbjct: 189 SVAIFGLGGIGLAAIQGARQAKAGRIIAIDTNPAKFELARTFGATECLNPKDFDKPIHEV 248 Query: 254 VKALTRGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPNQNWALQQVSLVAEER 312 + +T GVD+ FE G+V M +A RG G ++ G+ + + + LV R Sbjct: 249 LIEMTGWGVDHTFECIGNVNVMRSALEAAHRGWGQSIVIGVAGAGKEISTRPFQLVT-GR 307 Query: 313 TVKGSYIGSCVPVRDIPRYIGLYQAGKLPVDKLMGQRLALADINRGFDRLHSGEGLRDLI 372 T KGS G +P + G++ + + + L +IN FD +H G+ +R +I Sbjct: 308 TWKGSAFGGVKGRTQLPGMVEDAMKGEIDLAPFVTHTMGLDEINHAFDLMHEGKSIRTVI 367 Query: 373 VF 374 + Sbjct: 368 HY 369 >gi|134094301|ref|YP_001099376.1| putative glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 368 Score = 207 bits (528), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 132/377 (35%), Positives = 199/377 (52%), Gaps = 12/377 (3%) Query: 1 MKIHAAVLREMTEAHPFAVSQPLKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRP 60 MK AAV + QPL IE+++L P GEVLV++KA G+CH+D ++G P Sbjct: 1 MKTKAAV--------AWKAGQPLTIEEVELGGPREGEVLVEIKATGICHTDYYTLSGADP 52 Query: 61 RPV-PMALGHEASAEVVQVGSGVTDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 + P LGHE + VV+VG+GV L+ D V+ ++ P C C C+ + LC+ +T Sbjct: 53 EGIFPAILGHEGAGVVVEVGAGVKSLKKDDHVIPLYTPECRECKFCLSRKTNLCQKIRST 112 Query: 120 NTVGQLLSGQRRLHDGSGHVHHHLGVSAFADHAVVSSRSCVKIEADIDPVEAALFGCAVL 179 G + R + H++G S F+++ VV + KI D + GC V Sbjct: 113 QGRGLMPDATSRFSIDGKPLFHYMGTSTFSNYIVVPEIALAKIREDAPFDKVCYIGCGVT 172 Query: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALGA 239 TGVGA + TA+V+AG V GLGG+GL + A GA ++I VDL+ ++ +AR G Sbjct: 173 TGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGADKIIGVDLNPAREAMARKFGM 232 Query: 240 TATVN-ARDPDVVEKVKALTRGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPN 297 T +N ++ +VV+ + LT GG DY+FE G+V M A +G G ++ G+ Sbjct: 233 THFINPSKVENVVDAIVQLTDGGADYSFECIGNVHTMRQALECCHKGWGQSIVIGVAEAG 292 Query: 298 QNWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKLPVDKLMGQRLALADINR 357 + + LV R KGS G D+P+ + Y GKL +D L+ L L IN Sbjct: 293 AEISTRPFQLVT-GREWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHTLPLERINE 351 Query: 358 GFDRLHSGEGLRDLIVF 374 GFD + SGE +R ++++ Sbjct: 352 GFDLMKSGESIRSVVIY 368 >gi|134095240|ref|YP_001100315.1| glutathione-independent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 414 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 73/287 (25%), Positives = 113/287 (39%), Gaps = 34/287 (11%) Query: 35 PGEVLVKMKAAGLCHSDLSVITGVRPRPVPMALGHEASAEVVQVGSGVTDLRAGDLVVLV 94 P ++K+ +C SDL + G P M +GHE + EV++VGS V ++ GD+V + Sbjct: 46 PHGAILKIVTTNICGSDLHIYRGSFAVPKGMTMGHEMTGEVIEVGSDVEVVKKGDIVSVP 105 Query: 95 FVPSCGHCLPCMEGRPALCEP-------GAATNTVGQLLSGQRRLHDGSGHVHHHLGVSA 147 F CG C C R +CE GA +G GQ D + + Sbjct: 106 FNVGCGRCYNCKHMRSDVCENTNPEIDCGAYGFNLGGWTGGQ---GDYLFVPYADFNLLR 162 Query: 148 FADH-AVVSSRSCVKIEADIDPVEAALFGCAVLTGVGAAVNTAEVKAGTTAAVLGLGGVG 206 F D A + + + +D+ P F A V G + G G VG Sbjct: 163 FPDKDAAMEKIRDLTLLSDVLPTAFHGFAGPDWPAAPAYV------VGENILIFGAGPVG 216 Query: 207 LCAMLGALASGAREVIAVDLHDSKLEVARALGATATVNARD----PDVVEKV-------K 255 A GA +I D +L++ + G T+N D + +E++ + Sbjct: 217 RAGAACARLLGAGAIIVADYIQERLDLLKPHG-VETINLSDGVPIEEHLERITGHREVDR 275 Query: 256 ALTRGGVD---YAFEMAGSVQA--MEAAYRMTRRGGMTVTAGLPAPN 297 + GVD + E V++ A + R GGMT T G+ N Sbjct: 276 VIDYVGVDCRGFGAEADKIVESAVTNAMLKYVRFGGMTSTVGVYCAN 322 >gi|134094820|ref|YP_001099895.1| putative alcool dehydrogenase with NAD(P)-binding [Herminiimonas arsenicoxydans] Length = 352 Score = 54.7 bits (130), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 70/228 (30%), Positives = 99/228 (43%), Gaps = 19/228 (8%) Query: 22 PLKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRPRPV-PMALGHEASAEVVQVGS 80 P + E+ D +V++ + G+CHSD+ V PM GHE V VG Sbjct: 19 PFRFERRD---QRSNDVVIDILYCGVCHSDIHNARNDWGGAVYPMVPGHEIIGRVASVGV 75 Query: 81 GVTDLRAGDLV-VLVFVPSCGHCLPCMEGRPALCEPGAATNTVGQLLSGQRRLHDGSGHV 139 VT + GD V V V SC HC C + C G +T T + HD Sbjct: 76 NVTRFKVGDKVGVGCMVDSCQHCAACQKNLEQYCAEG-STFTYNAI-----DRHD----- 124 Query: 140 HHHLGVSAFADHAVVSSRSCVKIEADIDPVEAALFGCAVLTGVGAAVNTAEVKAGTTAAV 199 H +++ VVS + +K+ +D AA CA +T + + +V G+ AV Sbjct: 125 -HMPTYGGYSERIVVSDKFVLKVPDGLDLAGAAPLLCAGIT-TWSPLRHWKVGKGSKVAV 182 Query: 200 LGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALGATATVNARD 247 +GLGG+G + A A GA EV K + AR LGA V + D Sbjct: 183 VGLGGLGHMGLKLANALGA-EVTLFTRSPGKEQDARRLGAHHVVLSTD 229 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 630,291 Number of extensions: 23996 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 5 Length of query: 374 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 291 Effective length of database: 726,862 Effective search space: 211516842 Effective search space used: 211516842 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)