BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2746.p (524 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094220|ref|YP_001099295.1| major facilitator superfamily m... 606 e-175 gi|134096340|ref|YP_001101415.1| putative multidrug resistance-l... 130 1e-31 gi|134095839|ref|YP_001100914.1| bicyclomycin resistance protein... 67 2e-12 gi|134094141|ref|YP_001099216.1| MFS family transporter [Hermini... 59 4e-10 >gi|134094220|ref|YP_001099295.1| major facilitator superfamily multidrug transporter; membrane protein [Herminiimonas arsenicoxydans] Length = 517 Score = 606 bits (1563), Expect = e-175, Method: Compositional matrix adjust. Identities = 299/509 (58%), Positives = 397/509 (77%), Gaps = 4/509 (0%) Query: 4 SPTPPKPYTPPPPLSGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTW 63 S TP TP L+G LV+GT+++SLAVFMNVLDSSIANV+IP ISG+LGVS +GTW Sbjct: 2 SATPAAALTP---LNGTRLVVGTVALSLAVFMNVLDSSIANVSIPAISGDLGVSPQQGTW 58 Query: 64 VITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQG 123 VITSFA ANAIS+PLTGWLTQR G VRLF++S+LLFVLAS LCGLAP++ IL+ ARVLQG Sbjct: 59 VITSFAVANAISVPLTGWLTQRFGQVRLFISSILLFVLASCLCGLAPSIEILIAARVLQG 118 Query: 124 LVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYI 183 VAGPM+PLSQ+LLL +YP +KS ALA WGMT +VAP+ GP LGGWI+D+YTWPWIFYI Sbjct: 119 AVAGPMIPLSQALLLSSYPPAKSGMALAFWGMTTLVAPIMGPLLGGWISDNYTWPWIFYI 178 Query: 184 NIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPT 243 NIP+G+ AA T +IYR RE+ T+KLPIDK+GL LLV WV + Q+MLDKGK+LDWF S Sbjct: 179 NIPIGIFAAWATWSIYRERESKTYKLPIDKVGLVLLVLWVGSLQLMLDKGKELDWFHSGE 238 Query: 244 IVTLGLIALVAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQ 303 I+ L +A VAF YF++WE+ +HPVVDL LF RNF+GG IAIS+ YG+FFG+LVILP Sbjct: 239 IIILASVAAVAFIYFIIWEIGEEHPVVDLSLFQGRNFSGGVIAISVAYGLFFGSLVILPL 298 Query: 304 WLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSR 363 WLQ +GY + ++G AP+GIFA+IL P++G+ LP +DAR VAT +F+ F++VF++R+ Sbjct: 299 WLQTQIGYTATEAGKVMAPVGIFAIILSPIVGKLLPKIDARWVATTAFLIFSLVFFMRAE 358 Query: 364 YNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGT 423 + VD MT+++PT++QG AMFF+PLT ++LSG P++IPAAAGLS+FVR+ G +GT Sbjct: 359 FTQDVDMMTLMIPTVIQGAAMAMFFIPLTSIILSGQSPDKIPAAAGLSNFVRIMFGGIGT 418 Query: 424 SISTTAWNNRTILHHAQLTEHATPYSPWLQESVNNISSTLGLNAEQTAGMLERTITFQAA 483 S+ +T W+NRTILHHAQL E+ ++ ++VN + + G++ + +++R +T QA+ Sbjct: 419 SVMSTMWDNRTILHHAQLAEYTGAHNSAFTQAVNTLMAR-GMSEQAAWAVIDRQMTVQAS 477 Query: 484 MLGINDIFYVSAVIFIVIIPLIWIIKPSK 512 L D+F++S ++F+ +I IWI K SK Sbjct: 478 TLAATDLFWMSGIMFLCLIGFIWITKRSK 506 >gi|134096340|ref|YP_001101415.1| putative multidrug resistance-like efflux transmembrane protein [Herminiimonas arsenicoxydans] Length = 472 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 119/413 (28%), Positives = 198/413 (47%), Gaps = 22/413 (5%) Query: 38 LDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVL 97 L ++I NVAIP +S + + WV TSF A +S+ T WL QR G R + +V Sbjct: 31 LSATIVNVAIPDLSHYFTLGQERAQWVSTSFMLAMTLSMLTTPWLLQRFGLRRTYTGAVS 90 Query: 98 LFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTA 157 L +L + G + P+LL RVL+GL AG M P+ ++L A+ + A+ ++G Sbjct: 91 LLLLGGVVGGFSVNYPMLLSMRVLEGLAAGVMQPIPAIVILRAFAAEEQGKAMGIFGFGV 150 Query: 158 VVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYR----SRETPTHKLPIDK 213 V+ P GP++GG++ + + W IF++ +P L+ V I R + H P+D Sbjct: 151 VLTPAIGPSVGGFLVELFGWRSIFFVVVPPCLL---VFYMIRRFLPLYSQILEHAKPLDW 207 Query: 214 IGLALL-VTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVWELNNDH-PVVD 271 GL L+ ++ ++ +++ DL ++ +G +F F+V++L H P+V+ Sbjct: 208 KGLLLIGISIISLLNGLVELRADLQQALLLLLLGVG-----SFTAFIVYQLRTRHEPLVN 262 Query: 272 LRLFGRRNFA-GGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIFAVIL 330 + LFG R FA G +A G G+ FG+ +LP +LQ L Y +GL P GI + Sbjct: 263 MALFGYRQFAMGALVAFIYGMGL-FGSTYLLPVYLQVALHYAPSQAGLVLLPAGIVLALT 321 Query: 331 MPLIGRFLPLVDARKVATASFIGFAIV---FWLRSRYNLQVDQMTVILPTLLQGIPTAMF 387 +PL GR L D IG ++ F L + + + ++ ++ I Sbjct: 322 IPLAGR---LADRYPPNVLVVIGLGLLTASFVLMANGSSATSYLLLMAWAVIGRIGLGFV 378 Query: 388 FVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRTILHHAQ 440 L++ + GL + I +F R GA+G S+ R +H A+ Sbjct: 379 LPALSLGAMRGLDIKLIAQGVSALNFARQLGGAMGVSLVGIVLEWRITVHEAR 431 >gi|134095839|ref|YP_001100914.1| bicyclomycin resistance protein [Herminiimonas arsenicoxydans] Length = 422 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 50/168 (29%), Positives = 81/168 (48%), Gaps = 6/168 (3%) Query: 46 AIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWL 105 A P I +LG S E +T++ + A+ I G L+ G + + S+ +F +A+ Sbjct: 43 AFPNIQTSLGASALEVQQTLTAYLFSFAVMILWHGALSDAFGRRNIILVSLAVFAVATLG 102 Query: 106 CGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGP 165 C A ++ L R+LQG+ AG V + ++++ Y +++ L+L M +AP P Sbjct: 103 CAAAHSIEYLWAFRMLQGISAGAGVVIGRAIIRDLYAGAEAEKLLSLVTMIFSIAPAIAP 162 Query: 166 ALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDK 213 LGGWI W IF + L A V L +Y + P LP +K Sbjct: 163 MLGGWIVKRMDWRAIF-----LALFAYAVLLLLYCYKRLP-ESLPPEK 204 >gi|134094141|ref|YP_001099216.1| MFS family transporter [Herminiimonas arsenicoxydans] Length = 402 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 45/167 (26%), Positives = 78/167 (46%), Gaps = 2/167 (1%) Query: 47 IPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLC 106 +P ++ +S+ ++VIT+F+ A S G L R G + + + L + LC Sbjct: 40 LPRLAQEFDISLGSVSYVITAFSIAYGFSQLFFGPLGDRFGKYLVIAWACVACTLTALLC 99 Query: 107 GLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPA 166 LAP P+L+ AR+L G A +VPLS + + P + LA + + +V G Sbjct: 100 ALAPNYPLLIVARLLAGATAAAIVPLSMAWIGDVVPYDRRQPVLAQFLIGQIVGISTGVL 159 Query: 167 LGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDK 213 GG D ++W F+ I + + +TL + +R P + + K Sbjct: 160 FGGLAADYFSWRVPFF-GISLCFVLIALTLFSF-NRRLPAYAMTTHK 204 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.324 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 927,945 Number of extensions: 39871 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 5 Length of query: 524 Length of database: 1,000,347 Length adjustment: 86 Effective length of query: 438 Effective length of database: 716,977 Effective search space: 314035926 Effective search space used: 314035926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)