BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2586.p (260 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimo... 149 2e-37 gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/is... 90 1e-19 gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimo... 84 6e-18 >gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 258 Score = 149 bits (375), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 97/261 (37%), Positives = 138/261 (52%), Gaps = 11/261 (4%) Query: 4 YTNLKLEKHGHTAVLTLSNPPA-NTWTRQALSDLTRLVKELNAEREIYTLVVTGEGEKFF 62 Y N+ +E HG ++TL+ P A N + + +L + + +A +I +++TG EK F Sbjct: 3 YENILIEIHGKVGLITLNRPAALNALNDKLMDELGKALLSFDANEDIGCMIITGS-EKAF 61 Query: 63 SAGADLKLFADGDKAMAREMARRFGEAF----ETLSSFRGVSIAAINGYAMGGGLECALA 118 +AGAD+ G A M G+ E + S R IAA+ GYA+GGG E A+ Sbjct: 62 AAGADI-----GAMATYSYMDVYKGDYITRNWEQIRSVRKPVIAAVAGYALGGGCELAMM 116 Query: 119 CDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGL 178 CD IA + A+ PE +G++P AGGTQ LP V + A M L ++A A R GL Sbjct: 117 CDFIIAADTAKFGQPEIKLGIIPGAGGTQRLPRSVSKSKAMDMCLTARMMDAAEAERAGL 176 Query: 179 VEEVVPRGQARERALALAQQAQKQSPSSVAASKKLIQGARHNPLGTILQTERELFVDLFD 238 V VV + +E ALA A S V K+ I A L +Q ER LF F Sbjct: 177 VSRVVAADKLQEEALAAATVIASMSLPMVMMIKESINRAYETTLAEGVQFERRLFHSTFA 236 Query: 239 TQDQQEGVQAFLEKRPPQWKN 259 T+DQ+EG++AF+EKR ++KN Sbjct: 237 TEDQKEGMRAFVEKRAAKFKN 257 >gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/isomerase family protein [Herminiimonas arsenicoxydans] Length = 301 Score = 89.7 bits (221), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 76/259 (29%), Positives = 119/259 (45%), Gaps = 11/259 (4%) Query: 7 LKLEKHGHTAVLTLSNPPA-NTWTRQALSDLTRLVKELNAEREIYTLVVTGEGEKFFSAG 65 ++ +H T +LTLSNP A N + + I +++TG + FF AG Sbjct: 47 IRASRHEATLILTLSNPGAKNALHPDMFAAAIETFSTAARDDSIRAVILTG-ADNFFCAG 105 Query: 66 ADLKLFAD------GDKAMAREMARRFGEAFETLSSFRGVSIAAINGYAMGGGLECALAC 119 +L + D+A A + R + EA IAA+ A G G ALAC Sbjct: 106 GNLNTLLELRAQDRADQADAVDSLRGWIEAIRDCPK---PVIAAVESAAAGAGFSLALAC 162 Query: 120 DIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLV 179 D+ +A A+ A+ +GL P G + L + A ++L G+ V A+ +G++ Sbjct: 163 DLIVAGANAKFAMSYVNIGLTPDGGASWFLSQALPRQLATEIMLEGKPVPAQRLHDLGVI 222 Query: 180 EEVVPRGQARERALALAQQAQKQSPSSVAASKKLIQGARHNPLGTILQTERELFVDLFDT 239 +V G + +L A + S +V K L + AR + L + E+ FVD + Sbjct: 223 NRIVADGATLQTSLDWADELAALSGHAVERIKSLTREARDHSLPQHFEIEKNHFVDSLNH 282 Query: 240 QDQQEGVQAFLEKRPPQWK 258 +D QEG+ AFLEKR P +K Sbjct: 283 RDAQEGIHAFLEKRAPHYK 301 >gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 264 Score = 84.3 bits (207), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 73/258 (28%), Positives = 117/258 (45%), Gaps = 11/258 (4%) Query: 7 LKLEKHGHTAVLTLSNPPA-NTWTRQALSDLTRLVKELNAEREIYTLVVTGEGEKFFSAG 65 + L A + P A N T ++L + + + A++ I G G + F AG Sbjct: 12 VHLAIQDGIAAILFDRPQARNAMTWAMYTELGAICERIRADQSIRVTTFRGAGGEAFVAG 71 Query: 66 ADL---KLFADGDKAMAREMARRFGEAFETLSSFRGVSIAAINGYAMGGGLECALACDIR 122 D+ + F+ G+ +A E R E + + ++A ++G+A+GGGL A ACD R Sbjct: 72 TDIEQFRNFSSGEDGIAYE--RIVDERIGQIEALPMPTLAIVDGWAIGGGLAIAAACDFR 129 Query: 123 IAEEQAQMALPEA-AVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEE 181 IA ++ +P A +G + G G AKR++L E + A+ L G V E Sbjct: 130 IATPASRFGIPIARTLGNCLSMANIARVVAAFGVGRAKRILLLAEVLAADELLAAGFVTE 189 Query: 182 VVPRGQARERALALAQQAQKQSPSSVAASKKLIQGARHNPLGTILQTERELFVDLFDTQD 241 VV A AL ++ +P ++ SK+ I+ R G L +L + + D Sbjct: 190 VVEAAVLDATADALCKKLASNAPITMGVSKEAIR--RLTQAG--LPDGEDLIRACYGSSD 245 Query: 242 QQEGVQAFLEKRPPQWKN 259 + GV AFLEK+ P W Sbjct: 246 FKIGVHAFLEKQRPLWSG 263 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.317 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 462,531 Number of extensions: 17686 Number of successful extensions: 71 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 4 Length of query: 260 Length of database: 1,000,347 Length adjustment: 80 Effective length of query: 180 Effective length of database: 736,747 Effective search space: 132614460 Effective search space used: 132614460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)