BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2545.p (332 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094049|ref|YP_001099124.1| putative glyoxylate reductase (... 125 2e-30 gi|134096589|ref|YP_001101664.1| formate dehydrogenase [Herminii... 109 2e-25 gi|134096490|ref|YP_001101565.1| putative phosphoglycerate dehyd... 102 3e-23 gi|134096368|ref|YP_001101443.1| phosphonate dehydrogenase [Herm... 100 2e-22 gi|134096605|ref|YP_001101680.1| glyoxylate reductase [Herminiim... 91 6e-20 >gi|134094049|ref|YP_001099124.1| putative glyoxylate reductase (glycolate reductase) [Herminiimonas arsenicoxydans] Length = 327 Score = 125 bits (315), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 84/260 (32%), Positives = 135/260 (51%), Gaps = 12/260 (4%) Query: 33 QDRLDSSSA--QLAQGCEAVAAFVNDDLGAAVLERLAAQGVRFITTRSTGFNHIDTQAAR 90 +DR+ S + + QG + V ++ L V+ A ++ I + G+N+ID AA Sbjct: 30 EDRIFSPAELHEKMQGKDGVIITSSEKLSGEVI--TANPQLKAICNVAVGYNNIDVAAAT 87 Query: 91 RLGISVARVADYSPYSVAEFAVGLLLAVNRKIARASMRTREG---NFELEGLMGFDLHGK 147 + GI V D + A++A LL+A R+ + + R G N+ + +G D+HG Sbjct: 88 KAGIMVTNTPDVLNETTADYAWTLLMANARRTSESEHYLRAGKWKNWRFDQFLGADVHGA 147 Query: 148 TVGVIGTGKIGALFARIMAGFGCTLL---GSDMYQNPVFEALGGRYVDKQALFAASDVIS 204 T+G++G G+IG AR GF ++ S + + A RYV K+ L +D + Sbjct: 148 TLGILGMGRIGQAIARRSMGFDMQVIYHNRSRLTPDQEAHANNARYVSKEELLRTADHLI 207 Query: 205 LHCPLTEATRYIVNAESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQLRGLAIDVYEQ 264 L P +E T +I+ A LA KP LVN +RGG+VD A+IAAL+ ++ +DVYE Sbjct: 208 LVLPYSEQTHHIIGAAELALMKPTATLVNIARGGIVDDVALIAALREHRIASAGLDVYEN 267 Query: 265 EASLF--FQDLSSTIITDDV 282 E +L F LS+ ++T + Sbjct: 268 EPALHPDFLTLSNVVLTPHI 287 >gi|134096589|ref|YP_001101664.1| formate dehydrogenase [Herminiimonas arsenicoxydans] Length = 400 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 75/246 (30%), Positives = 128/246 (52%), Gaps = 9/246 (3%) Query: 25 NTHELVFLQDRLDSSSAQLAQGCEAVAAFVNDDLGAAVL--ERLA-AQGVRFITTRSTGF 81 N H LV + D + + L + ++ A + ER+A A+ ++ I T G Sbjct: 67 NGHTLVVTSSK-DGADSVLDKELHDAEIIISQPFWPAYMTAERIAKAKKLKMIVTAGIGS 125 Query: 82 NHIDTQAARRLGISVARVADYSPYSVAEFAVGLLLAVNRKIARASMRTREGNFELEGLMG 141 +H D +AA + I+VA V + +SVAE + ++L+ R + + +G + + + Sbjct: 126 DHTDLEAANKHNITVAEVTYCNSHSVAEHVLMMILSQVRNYIPSYKQVIDGGWNIADCVS 185 Query: 142 --FDLHGKTVGVIGTGKIGALFARIMAGFGCTLLGSDMYQNP--VFEALGGRYVDK-QAL 196 +DL +VG + G+IG R++ F L D ++ P V + L Y ++L Sbjct: 186 RSYDLEAMSVGTVAAGRIGLRVLRLLHPFDVKLHYMDRHRLPTAVEKELNLTYHSTLESL 245 Query: 197 FAASDVISLHCPLTEATRYIVNAESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQLRG 256 DV++L+CPL T +++N ++L K G ++NT+RG L D +A++AALKSGQL G Sbjct: 246 TKVCDVVTLNCPLHPETEHMINEKTLKNFKRGAYIINTARGKLCDRDAIVAALKSGQLAG 305 Query: 257 LAIDVY 262 A DV+ Sbjct: 306 YAGDVW 311 >gi|134096490|ref|YP_001101565.1| putative phosphoglycerate dehydrogenase(partial) [Herminiimonas arsenicoxydans] Length = 317 Score = 102 bits (254), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 81/243 (33%), Positives = 121/243 (49%), Gaps = 17/243 (6%) Query: 67 AAQGVRFITTRSTGFNHIDTQAARRLGISVARVADYSPYSVAEFAVGLLLAVNRKIARAS 126 AA +R + G ++ID A + GI V + +VAE+ +G +A+ R+ S Sbjct: 69 AAPKLRVVGRLGVGLDNIDVDACKARGIDVFPATGANAGAVAEYVIGTAMALLREAYTRS 128 Query: 127 MRTREGNFELEGLM-GFDLHGKTVGVIGTGKIGALFARIMAGFGCTLLGSDMYQNP---V 182 T G + L G ++ GKT+G+IG G IG L AR+ G T++ D P + Sbjct: 129 AETAAGTWPRTALSNGREIAGKTLGLIGFGGIGQLTARLAQALGVTVIAHDPLLAPDAAI 188 Query: 183 FEALGGRYVDKQALFAASDVISLHCPLTEATRYIVNAESLAATKPGCILVNTSRGGLVDT 242 ++ G + +D++SLH PLT TR ++ A++LA KP +L+NTSRG +VD Sbjct: 189 WQQAGVACKQLHEVLEQADIVSLHIPLTTNTRNLIGADALARMKPDAVLINTSRGEIVDE 248 Query: 243 EAVIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFT 302 A+ AL S L G AIDV+ QE L++ P VI+T H A T Sbjct: 249 AALAQALHSANLGGAAIDVFAQEPLAPGNALANA-------------PRVILTPHIAGVT 295 Query: 303 EEA 305 E+ Sbjct: 296 TES 298 >gi|134096368|ref|YP_001101443.1| phosphonate dehydrogenase [Herminiimonas arsenicoxydans] Length = 336 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 73/226 (32%), Positives = 111/226 (49%), Gaps = 6/226 (2%) Query: 44 AQGCEAVAAFVNDDLGAAVLERLAAQGVRFITTRSTGFNHIDTQAARRLGISVARVADYS 103 ++ +AV AF+ D + + L A ++ + G+++ D A + G+ V D Sbjct: 43 SKDADAVMAFMPDSVDDSFLA--ACPKLKIVFAALKGYDNFDVDACTKRGVWFGIVPDLL 100 Query: 104 PYSVAEFAVGLLLAVNRKIARASMRTREGNFE--LEGLMGFDLHGKTVGVIGTGKIGALF 161 AE VGLLL + R + R G F L G L G T+G+IG G++G Sbjct: 101 TVPTAELTVGLLLGLTRHVMAGDDHVRSGTFHGWRPKLYGAGLAGSTIGIIGMGRVGKAI 160 Query: 162 ARIMAGFGCTLLGSDMYQ-NPVFE-ALGGRYVDKQALFAASDVISLHCPLTEATRYIVNA 219 A+ ++GF + D NPV E A R V L SD + P+T T ++++A Sbjct: 161 AKRLSGFEMNAVYCDSVPLNPVDEQAWNARQVSFDELLTCSDFVVPMLPMTSDTFHLIDA 220 Query: 220 ESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQLRGLAIDVYEQE 265 +++ + G L+NTSRG +VD AV+ AL G L G A DV+E E Sbjct: 221 HAISKMRRGSYLLNTSRGSVVDENAVVEALNQGHLAGYAADVFEME 266 >gi|134096605|ref|YP_001101680.1| glyoxylate reductase [Herminiimonas arsenicoxydans] Length = 327 Score = 91.3 bits (225), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 74/250 (29%), Positives = 120/250 (48%), Gaps = 24/250 (9%) Query: 83 HIDTQAARRLGISVAR-VADYSPYSVAEFAVGLLLAVNRKIARASMRTREGNFELEGL-- 139 HID AA I++ V D P + AE L++A RK+ + ++G +++ L Sbjct: 82 HIDLSAAAARNITIVEGVGD--PTAPAELTWALIMAAMRKLPQYVGNLKDGLWQIASLSS 139 Query: 140 ----MGFDLHGKTVGVIGTGKIGALFARIMAGFGCTLL--GSDMYQNPVFEALGGRYVDK 193 +G L G+T+G+ G G+IG + A FG +L G D + + + Sbjct: 140 AHNTLGAVLKGRTLGIWGYGRIGRMVAGYGQAFGMKVLVWGRDASRAAAVQDGYQAASSR 199 Query: 194 QALFAASDVISLHCPLTEATRYIVNAESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQ 253 + F+ +DV+SLH L +ATR IV A LA K + VNTSR LV ++A+ AL+ G+ Sbjct: 200 EEFFSTADVLSLHLRLNDATRGIVTASDLAHMKSSALFVNTSRAELVASDALQTALEQGR 259 Query: 254 LRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFTEEAIGQIMQTT 313 A+DV+E E L+ T L+ NV+ + H + +++ ++ Sbjct: 260 PGYAALDVFESEP------LAPT-------SSLLRMENVLASPHLGYVEKDSYELYFRSA 306 Query: 314 IANLSDFAAG 323 N+ FA G Sbjct: 307 FENIVAFANG 316 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.322 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 573,008 Number of extensions: 22888 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 5 Length of query: 332 Length of database: 1,000,347 Length adjustment: 82 Effective length of query: 250 Effective length of database: 730,157 Effective search space: 182539250 Effective search space used: 182539250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)