BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2501.p (873 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094989|ref|YP_001100064.1| aconitate hydratase [Herminiimo... 677 0.0 gi|134094444|ref|YP_001099519.1| isopropylmalate isomerase large... 89 7e-19 gi|134094018|ref|YP_001099093.1| isopropylmalate isomerase large... 84 3e-17 >gi|134094989|ref|YP_001100064.1| aconitate hydratase [Herminiimonas arsenicoxydans] Length = 912 Score = 677 bits (1748), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/876 (42%), Positives = 530/876 (60%), Gaps = 50/876 (5%) Query: 34 LPYTSRVLAENLVRRCDPATLTDS-LRQLIE----RKRELDFPWFPARVVCHDILGQTAL 88 LP + R++ E+++R CD +T+ + QL R + P+ ARVV D G L Sbjct: 39 LPISIRIVLESVLRNCDGKKVTEEHIHQLANWSPTATRTDEIPFVVARVVLQDFTGVPLL 98 Query: 89 VDLAGLRDAIADMGGDPAKVNPVVPVQLIVDHSLAVECGGDDPQAFEKNRAIEDRRNEDR 148 DLA +R A MG +P + P+VPV L+VDHS+ ++ A + N +E +RN +R Sbjct: 99 ADLAAMRGVAARMGKNPKNIEPLVPVDLVVDHSIQIDHF-RQKNALDLNMKLEFQRNNER 157 Query: 149 FHFIDWTKLAFENVDVIPPGNGIMHQINLEKMSPVIH-----AKDGVAFPDTLVGTDSHT 203 + F+ W AF+ V+PPG GI+HQ+NLE ++ +H K + +PDTLVGTDSHT Sbjct: 158 YQFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKRKNGGKTDIYYPDTLVGTDSHT 217 Query: 204 PHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGVELTGRRQSGITATDIVLSLTEFL 263 ++ +GV+ GVGG+EAE MLG+ + PD++GV LTG+ + G+TATD+VL++TE L Sbjct: 218 TMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGQLREGVTATDLVLTITELL 277 Query: 264 RKQKVVGAYLEFYGEGAASLTLGDRATISNMAPEYGATAAMFSIDQQTIDYLKLTGREDE 323 RK+KVVG ++EF+GEG SL+L DRATI+NMAPEYGAT F +D TIDY K TGR Sbjct: 278 RKEKVVGKFVEFFGEGTESLSLTDRATIANMAPEYGATMGFFPVDDATIDYFKGTGRTKA 337 Query: 324 QVKLVETYAKEAGLWS-DTLAKAEYERVLRFDLSTVVRTLAGPSNPHKRLPVSEL----- 377 ++ E Y K GL+ + +Y V+ DL +V +LAGP P R+ + + Sbjct: 338 EIDAFENYFKAQGLYGIPKSGEIDYTHVVALDLGSVAPSLAGPKRPQDRIEIGNVKQNFA 397 Query: 378 -------AAQGIAGKVE--------KEPGKMPDGAVIIAAITSCTNTSNPRNVIAAALLA 422 + G K E + K+ +G V+IAAITSCTNTSNP ++ A LLA Sbjct: 398 DLFAKPTSENGFNKKAEDLDATYTNSDGVKVKNGDVLIAAITSCTNTSNPSVLLTAGLLA 457 Query: 423 RNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGMSGAL 482 + A GL P +K+SLAPGS+ V YL+ AGL LEKLGFG+ A+ CTTC G +G L Sbjct: 458 KKAVEAGLKVSPHIKTSLAPGSRVVTEYLQAAGLLPYLEKLGFGVTAYGCTTCIGNAGDL 517 Query: 483 DPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIEQD 542 P + + I+ D+ ++AVLSGNRNF+ RIHP + FLASPPLVVAYAIAG + D+ + Sbjct: 518 TPAMNEAIVQNDIVASAVLSGNRNFEARIHPNIRSNFLASPPLVVAYAIAGNMTRDLMTE 577 Query: 543 VL----GVTAEGREIRLKDIWPSDEEIDAVVAAAVKPQQFRNVYTPMFAARVDRSQSVS- 597 + G + R++ L DIWP+ +E+ ++ A+ + F++ Y + A + + Sbjct: 578 PVGRGKGADGKMRDVYLGDIWPTSQEVSKLMKFAMNAKVFKDNYAQVKTAPGKLWEGIKG 637 Query: 598 ----PLYDWRAQSTYIRRPPYWEGAL----AGARTLTGMRPLAVLGDNITTDHLSPSNAI 649 +YDW STYI PP++ A A +T R L V GD+ITTDH+SP+ +I Sbjct: 638 VAEGQVYDW-PSSTYIAEPPFFNDFTMEPKAAATGITAARALGVFGDSITTDHISPAGSI 696 Query: 650 LLDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVK-KADGSVQQGS 708 S AG+YL + G+ + DFNSY + RG+H R TFAN ++ N M+ K DGS +G Sbjct: 697 KDTSPAGKYLTEKGVLKADFNSYGSRRGNHEIMMRGTFANVRIKNLMIPIKPDGSRVEGG 756 Query: 709 LARLEPEGRVMRMWETIETYMERKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEG 768 + R +P G M +++ Y+ P ++ AG +YG GSSRDWAAKG +L GV+A++A Sbjct: 757 ITRFQPTGEEMSIYDAAMRYVSEGTPTMVFAGEEYGTGSSRDWAAKGTQLLGVKAVIARS 816 Query: 769 FERIHRTNLIGMGVLPLEFKPGENRNTYAIDGTETYDVI---GQRRPRADLTLVIHRKNG 825 +ERIHR+NL+GMGVLPL+F ++ T I G ET+D+ G RP+ ++TLVIHR NG Sbjct: 817 YERIHRSNLVGMGVLPLQFLGDDSVTTLGITGAETFDLKGLEGNLRPQQEVTLVIHRANG 876 Query: 826 ERVEVPVTCRLDTAEEVSIYEAGGVLQRFAQDFLEA 861 + +V V R+DT EV Y+ GG+L + L A Sbjct: 877 QSQDVKVLLRIDTPIEVDYYKHGGILPFVLRQLLAA 912 >gi|134094444|ref|YP_001099519.1| isopropylmalate isomerase large subunit [Herminiimonas arsenicoxydans] Length = 466 Score = 89.4 bits (220), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 111/404 (27%), Positives = 168/404 (41%), Gaps = 49/404 (12%) Query: 155 TKLAFENVDVIPPGNGIMHQINLEKMSPVIHA---KDGVAFPD-TLVGTDSHTPHVDALG 210 ++L E +D G+ + +K ++H + G P T+V DSHT A G Sbjct: 79 SRLQVETLDHNAHEYGLTYFSMRDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFG 138 Query: 211 VIAIGVGGLEAENVMLGRASWMRLPDIIGVELTGRRQSGITATDIVLSLTEFLRKQKVVG 270 +A G+G E E+V+ + + + V++ G G+TA DIVL++ + G Sbjct: 139 ALAHGIGTSEVEHVLATQTLLAQKSKAMLVQVDGALPVGVTAKDIVLAIIGKIGTAGGTG 198 Query: 271 AYLEFYGEGAASLTLGDRATISNMAPEYGATAAMFSIDQQTIDYLKLTGRE--------D 322 +EF G SL++ R TI NMA E GA A M ++D+ TI+Y+K GR D Sbjct: 199 YAIEFAGSTIRSLSMEGRMTICNMAIEAGARAGMVAVDETTINYVK--GRPLSPVGPHWD 256 Query: 323 EQVKLVETYAKEAGLWSD---TLAKAEYERVLRFDLS--TVVRTLAGPSNPHKRLPVSE- 376 V+ T + G D TL AE + + + S VV +P K ++ Sbjct: 257 RAVEYWRTLHSDVGAKFDLVVTLNAAEVKPQVSWGTSPEMVVSVDGRVPDPDKEKDATKR 316 Query: 377 ------LAAQGIAGKVEKEPGKMPDGAVIIAAITSCTNTSNPRNVIAAALLARNANRLGL 430 L G+ K + D + I SCTN S +++ AAA + R +R Sbjct: 317 DGMEKALVYMGL-----KPNTPITDIRIDKVFIGSCTN-SRIQDLRAAAAVVRGKHRASN 370 Query: 431 ARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGMSG-ALDPKIQQE 489 + V PGS V+ E GL + GF C+ C M+ L+P Sbjct: 371 VKLAMV----VPGSGLVKEQAEREGLDKIFKDAGFEWREPGCSMCLAMNADRLEPG---- 422 Query: 490 IIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG 533 + NRNF+GR + L SP + A I G Sbjct: 423 -------ERCASTSNRNFEGR-QGAGGRTHLVSPAMAAAAGIEG 458 >gi|134094018|ref|YP_001099093.1| isopropylmalate isomerase large subunit [Herminiimonas arsenicoxydans] Length = 470 Score = 84.0 bits (206), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 105/380 (27%), Positives = 158/380 (41%), Gaps = 44/380 (11%) Query: 177 LEKMSPVIHAKDGVAFPDTLV-GTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLP 235 L+ + VI + G+ P +V DSHT ALG + G+G E E+V+ + RL Sbjct: 105 LQGIEHVIAPEHGMIRPGMVVICGDSHTTTYGALGALGFGIGTTEVEHVLATQTLVYRLA 164 Query: 236 DIIGVELTGRRQSGITATDIVLSLTEFLRKQKVVGAYLEFYGEGAASLTLGDRATISNMA 295 + + + G+ +G TA D+VL + + Q G +EF G +L++ R T+ NMA Sbjct: 165 KNMRIRVDGKLPNGTTAKDLVLMIISRIGAQGARGFVVEFSGTAIDALSVEARFTLCNMA 224 Query: 296 PEYGATAAMFSIDQQTIDYL--KLTGREDEQVKLVETYAKEAGLWSDTLAKAEYERVLRF 353 E GA + + DQ +DY+ + V A L SD A + + V F Sbjct: 225 VEAGARGGLIAPDQAAVDYVMQRCPDIAANPAWRVAALADWQALRSDEDAAFDVDHV--F 282 Query: 354 DLSTVVRTLAGPSNPHKRLPVS----ELAAQGIAGKVEKEPGKMPDG----------AVI 399 + V + ++P + +P+S E + A K E G +V Sbjct: 283 AAADVAPFVTWGTSPDQAIPISGHIPECDQENDAEKESTERALRYTGLQVGMSLEGLSVK 342 Query: 400 IAAITSCTN--TSNPRNVIAAALLARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLT 457 I SCTN + R VIA + A V++ + PGS AV + E+ G+ Sbjct: 343 HVFIGSCTNGRIEDLRAVIATIGDRKVA--------AGVRAMVVPGSGAVRMQAEQEGIA 394 Query: 458 ADLEKLGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYS--TAVLSGNRNFDGRIHPYA 515 A L GF C+ C M+ D L S + NRNF+GR Sbjct: 395 AKLIAAGFEWRKPGCSMCLAMN------------DDVLASGTRCASTTNRNFEGR-QGRG 441 Query: 516 KQAFLASPPLVVAYAIAGTI 535 L SP + A A+ GTI Sbjct: 442 SITHLMSPAMAAAAAVTGTI 461 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,693,945 Number of extensions: 72876 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 3 Length of query: 873 Length of database: 1,000,347 Length adjustment: 90 Effective length of query: 783 Effective length of database: 703,797 Effective search space: 551073051 Effective search space used: 551073051 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)