BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2320.p (149 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095419|ref|YP_001100494.1| iron-sulfur template protein Is... 227 2e-61 >gi|134095419|ref|YP_001100494.1| iron-sulfur template protein IscU [Herminiimonas arsenicoxydans] Length = 134 Score = 227 bits (578), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 105/128 (82%), Positives = 118/128 (92%) Query: 1 MAYSDKLMDHYENPRNVGSFDKDDTSVATGMVGAPACGDVMKLQIKVDHNGIIEDAKFKT 60 MAYSDK++DHYENPRNVG+FDK D ++ TGMVGAPACGDVMKLQIKV +G+IEDAKFKT Sbjct: 1 MAYSDKVLDHYENPRNVGAFDKGDETIGTGMVGAPACGDVMKLQIKVGADGVIEDAKFKT 60 Query: 61 YGCGSAIASSSLVTEWLKGRTLDQALDIKNTAIADELALPPVKIHCSILAEDAIKAAVAD 120 YGCGSAIASSSLVTEW+KG+TLDQAL IKNT IA+ELALPPVKIHCSILAEDAIKAAV D Sbjct: 61 YGCGSAIASSSLVTEWVKGKTLDQALSIKNTQIAEELALPPVKIHCSILAEDAIKAAVLD 120 Query: 121 YRSRHDAA 128 Y+++H A Sbjct: 121 YKTKHGVA 128 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.316 0.131 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 249,378 Number of extensions: 8511 Number of successful extensions: 31 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 31 Number of HSP's successfully gapped: 1 Length of query: 149 Length of database: 1,000,347 Length adjustment: 74 Effective length of query: 75 Effective length of database: 756,517 Effective search space: 56738775 Effective search space used: 56738775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 124 (52.4 bits)