BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2223.p (1789 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 76 2e-14 gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 64 5e-11 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 75.9 bits (185), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 130/535 (24%), Positives = 218/535 (40%), Gaps = 76/535 (14%) Query: 1193 QLSYAQLEARANQMARQLLAMGVQPEQRIGLALERSLDMVPALLAILKAGACFVPLDPQY 1252 +++Y +L R + A L ++ + R+ + + S+D + A+ A + GA + + Sbjct: 113 RITYKELHERVCKFANGLKSLSINKGDRVIIYMPMSIDGIAAMQACARIGATHSVVFGGF 172 Query: 1253 PRARLTAMIEDSRIRLALTQSHM---REHLP------------GQAGVRWLELDRLPAPA 1297 L I D+ LT + ++LP G +R + + + A Sbjct: 173 SAKSLHERIIDAGAVAVLTADYQVRGGKNLPLKAIVDEALAMGGCDTIRNVVVYKRTGGA 232 Query: 1298 ATSDFMEAPDVITH-----------PAQLA-----YVIYTSGSTGRPKGVAVSHGALVEH 1341 DF+E D+ H P + +++YTSGSTG+PKGV S + Sbjct: 233 V--DFVERRDIWLHELVEQRTSECAPEPVGAEHPLFILYTSGSTGKPKGVQHSSAGYLLW 290 Query: 1342 TQLSAEFS-RLRNDDRMLQFATLNF-DGFIEQLFPPLLVGAS-VVLRG-PQLWSPEEFQQ 1397 L+ ++ ++ +D A + + G + PL VGA+ +V G P + F + Sbjct: 291 AMLTMKWVFDIKPNDVYWCTADIGWVTGHTYVTYGPLAVGATQIVFEGVPMYPNAGRFWE 350 Query: 1398 RLREHDISI-ADLPTAYWHALIQS-----FARQGQSDYGRLREIHIGGEAMPAES-IATW 1450 +++H +SI PTA +LI++ + D LR + GE + E+ + + Sbjct: 351 MVQKHKVSIFYTAPTAI-RSLIKAAEADPSTHPSKYDLSSLRLLGSVGEPINPEAWMWYY 409 Query: 1451 RQAGLSAVRLLNTYGPTE--AVVVASLLDCDPFVQGQREVPTQMPIGQPLAGRSLHVLDE 1508 + G +++T+ TE V++ L P V G + PL G V+D+ Sbjct: 410 KNIGGERCPIVDTFWQTETGGHVISPLPGATPMVPGSCTL--------PLPGIIAAVVDD 461 Query: 1509 RLQALPAGVPGELYIGGDL--LARGYLERPGLTAERFVAD--PGSEAGGRLYRTGDLVCW 1564 LP G G L I + R P ERF P E GGR Y GD Sbjct: 462 GGHDLPNGKGGLLVIKRPWPAMIRNIWNDP----ERFEKSYFP-HELGGRTYLAGDGAIR 516 Query: 1565 NAQSELHYL-GRSDLQVKLRGFRVELGEIEAQLLAQPEVRQAIVVAHQARLVGYA----- 1618 N ++ + GR D + + G R+ EIE+ L+A P V +A VV G + Sbjct: 517 NEETGYFTITGRIDDVLNISGHRMGTAEIESALVANPMVAEAAVVGKPDETTGESICAFV 576 Query: 1619 ---SLYP-GQELS--TAQLRERLRASLPDYMVPATVIVLEQLPTNPNGKIDRHAL 1667 + P G E +LR+ + + P + + LP +GKI R L Sbjct: 577 VLKRVRPEGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLL 631 Score = 59.7 bits (143), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 134/573 (23%), Positives = 225/573 (39%), Gaps = 87/573 (15%) Query: 32 DTALIVVQAVAGVDQERRFSYQVLAQRVRALAAVLQA-RLGRGERALILLDNDEYYAISM 90 D I+ +A G R +Y+ L +RV A L++ + +G+R +I + +M Sbjct: 98 DKVAIIFEADDG--NVTRITYKELHERVCKFANGLKSLSINKGDRVIIYMPMSIDGIAAM 155 Query: 91 FACFYAGVIAVPVFPPESASAAH-----TGRLAGIAADAGARG------LLILDANRALV 139 AC G VF SA + H G +A + AD RG I+D A+ Sbjct: 156 QACARIGATHSVVFGGFSAKSLHERIIDAGAVAVLTADYQVRGGKNLPLKAIVDEALAM- 214 Query: 140 AACEGLQQAEMIAVDQIGAGD----RGPW---------QAAAPDP---QEIAFLQYTSGS 183 C+ ++ ++ GA D R W AP+P + F+ YTSGS Sbjct: 215 GGCDTIRNV-VVYKRTGGAVDFVERRDIWLHELVEQRTSECAPEPVGAEHPLFILYTSGS 273 Query: 184 TSAPKGVMVTHAA-LMANEAAIREGLSIGEGDSFCVWSPLFHDMGLIGGLLQPFYSGIVC 242 T PKGV + A L+ ++ I D + W D+G + G Y G + Sbjct: 274 TGKPKGVQHSSAGYLLWAMLTMKWVFDIKPNDVY--WCTA--DIGWVTGHTYVTY-GPLA 328 Query: 243 VLCSPRYFLERPV-----RWLQMIARHRVSISGGPDFAYRLCLDRIK---DRQLEGLDLS 294 V + F P+ R+ +M+ +H+VSI A R + + DLS Sbjct: 329 VGATQIVFEGVPMYPNAGRFWEMVQKHKVSIFYTAPTAIRSLIKAAEADPSTHPSKYDLS 388 Query: 295 CWRVAYTGAEPVRHQTMEAFVQRFASVGLDPAAVYPCYGLAEATLFVTGGRRGAGLRVQR 354 R+ + EP+ EA++ + ++G + + + E TGG V Sbjct: 389 SLRLLGSVGEPINP---EAWMWYYKNIGGERCPIVDTFWQTE-----TGG------HVIS 434 Query: 355 YLPHALRSRLALPADAEEPAVALVACGRAPSQHQVRIAADASGAPAAPGGIGEIWVTGPS 414 LP A +P P ++A H + P GG+ I P+ Sbjct: 435 PLPGATP---MVPGSCTLPLPGIIAAVVDDGGHDL---------PNGKGGLLVIKRPWPA 482 Query: 415 IARGYWKQALQSAQTFV--ELENQTWLR-TGDLGFWADGELFVAGRLKDMIIVRGHNIYP 471 + R W + +++ EL +T+L G + G + GR+ D++ + GH + Sbjct: 483 MIRNIWNDPERFEKSYFPHELGGRTYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMGT 542 Query: 472 QDLEQALEAEVEALRKGRIAVFAVSLDTEEGVGVAVEVSRGMQKLVAPQA-----LCEAV 526 ++E AL A +A AV +E G ++ + + K V P+ + + + Sbjct: 543 AEIESALVA------NPMVAEAAVVGKPDETTGESI-CAFVVLKRVRPEGDEAKQIAKEL 595 Query: 527 ATVVQRQCGEAPQVLVLLNPGGLPKTSSGKLQR 559 V ++ G + + LPKT SGK+ R Sbjct: 596 RDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMR 628 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 64.3 bits (155), Expect = 5e-11, Method: Composition-based stats. Identities = 138/612 (22%), Positives = 224/612 (36%), Gaps = 132/612 (21%) Query: 10 LPFKGARNFVERLEQLARERPDDTALIVVQAVAGVDQERRFSYQVLAQRVRALAAVLQAR 69 + F + VE LE+ R+ D A + + ++ +Y + + L A LQ Sbjct: 17 IDFTQYDSLVELLEESFRKYADRHAYVCM--------DKFLTYAEVDTLSQKLGAWLQGT 68 Query: 70 -LGRGERALILLDNDEYYAISMFACFYAGVIAV---PVFPPESASAAHTGRLAGIAADAG 125 L +G R +++ N Y +++ A AG + V P++ P L D+G Sbjct: 69 GLQKGARVALMMPNVLQYPVALAAVLRAGYVVVNVNPLYKPR--------ELQHQLVDSG 120 Query: 126 ARGLLILDANRALVAACEGLQQAEMIAVDQIG---AGDRG-------------------P 163 A ++IL+ + Q + + V +G G +G P Sbjct: 121 AEAIIILENFATTLEKIIVSTQIKHVVVATMGDMLGGLKGLLVNFVVRHVKKMVPAFSLP 180 Query: 164 WQAAAPD----------------PQEIAFLQYTSGSTSAPKGVMVTH----AALMANEAA 203 + D PQ+IAFLQYT G+T KG M+TH A ++ NEA Sbjct: 181 QAVSFKDVLAQAAHMHLQRPLLGPQDIAFLQYTGGTTGTAKGAMLTHRNIVANILQNEAW 240 Query: 204 IREGLSIGEGD---SFCVWSPLFHDMGLIGGLLQPFYSGIVCVLC-SPR-------YFLE 252 I L D +F PL+H L + G++ +L +PR ++ Sbjct: 241 IWPVLRKEPPDKTLNFVCALPLYHIFALTACCMLGTRLGVMNILIPNPRDIPSLVKELMK 300 Query: 253 RPVRWLQMIARHRVSISGGPDFAYRLCLDRIKDRQLEGLDLSCWRVAYTGAEPVRHQTME 312 + L + + PDFA +D S ++ G V+ + Sbjct: 301 YKINLLPAVNTLYNGLLNNPDFA--------------KVDFSELKLCNGGGMAVQ----Q 342 Query: 313 AFVQRFASVGLDPAAVYPCYGLAEATLFVTGGRRGAGLRVQRYLPHALRSRLALPADAEE 372 R+ V ++ YGL+E + T + LP + E Sbjct: 343 VVNDRWRVV--TGCSIIEGYGLSETSPVATSNPVETT---------EFSGTIGLPVPSTE 391 Query: 373 PAVALVACGRAPSQHQVRIAADASGAPAAPGGIGEIWVTGPSIARGYWKQALQSAQTFVE 432 + D++ A G GEI + GP + GYW ++AQ Sbjct: 392 VMIL-----------------DSNDNEVALGLSGEIAIRGPQVMAGYWNNPAETAQA--- 431 Query: 433 LENQTWLRTGDLGFW-ADGELFVAGRLKDMIIVRGHNIYPQDLEQALEAEVEALRKGRIA 491 + + +TGD+G A G + R KDMI+V G N+YP ++E + L I Sbjct: 432 MTADGFFKTGDIGVMDARGYTRIVDRKKDMILVSGFNVYPSEIENVVAGHPGVLECACIG 491 Query: 492 VFAVSLDTEEGVGVAVEVSRGMQKLVAPQALCEAVATVVQRQCGEAPQVLVLLNPGGLPK 551 V D G V + V R L A EA+ + Q + ++ LPK Sbjct: 492 V----PDEHTGEAVKLFVVRRDPALTA-----EALMAFCKEQFTGYKKPKMIEFRTELPK 542 Query: 552 TSSGKLQRGACR 563 ++ GK+ R R Sbjct: 543 SNVGKILRRELR 554 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.321 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 3,400,766 Number of extensions: 144606 Number of successful extensions: 437 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 435 Number of HSP's successfully gapped: 4 Length of query: 1789 Length of database: 1,000,347 Length adjustment: 95 Effective length of query: 1694 Effective length of database: 687,322 Effective search space: 1164323468 Effective search space used: 1164323468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 136 (57.0 bits)