BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2184.p (381 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094823|ref|YP_001099898.1| putative RND divalent metal cat... 122 3e-29 gi|134093785|ref|YP_001098860.1| cobalt-zinc-cadmium resistance ... 113 1e-26 gi|134094811|ref|YP_001099886.1| cobalt-zinc-cadmium resistance ... 100 2e-22 gi|134096397|ref|YP_001101472.1| putative heavy metal efflux sys... 77 1e-15 gi|134094274|ref|YP_001099349.1| hypothetical protein HEAR1042 [... 62 5e-11 >gi|134094823|ref|YP_001099898.1| putative RND divalent metal cation efflux membrane fusion protein (czcBlike) [Herminiimonas arsenicoxydans] Length = 404 Score = 122 bits (306), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 89/293 (30%), Positives = 148/293 (50%), Gaps = 7/293 (2%) Query: 80 LPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSDERKAQS 139 LP ++ + R A ++P +G + S+ LG VK G VL I S+ L++ S+ AQ Sbjct: 93 LPGEIQFNEDRTAHVVPRLSGVVQSVAADLGQQVKKGQVLAVIASTGLSEQRSELLSAQK 152 Query: 140 TLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAARAHARVAALG-GTAASGGS 198 +AL R ER++ L + ISA +D QAQ +++ A ++AALG G+ +SG Sbjct: 153 RMALARLTFEREKKLWEEKISAEQDYLQAQQTLRESEIAVQNASQKLAALGAGSGSSGAF 212 Query: 199 ARLAVRAPVSGHVIELNAAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDA 258 R +RAP G V+ + A+G ++ A + T++DLSSV+ + KDL + +G+ Sbjct: 213 NRYEIRAPFDGMVVAKHIALGEAVKED-ADIFTISDLSSVWAEIAVSAKDLNAVRVGEKV 271 Query: 259 EIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQATLREQAHAG 318 + A+ + VG +L TRT R+ + N +PG+F + Sbjct: 272 IVKASAFDSSA-SGTIAYVGSLLGEQTRTATARVTLANPQGTWRPGLFVSIEIV-AGETS 329 Query: 319 LLVPMTAVVQSGFANR--VFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAGT 369 + V ++A ++ VFV+VA F A+++ G G VE+ G+QAG+ Sbjct: 330 VPVSVSAEALHSINDKPVVFVKVAD-GFIAQQVTTGRSDGKSVEIVKGMQAGS 381 >gi|134093785|ref|YP_001098860.1| cobalt-zinc-cadmium resistance protein CzcB [Herminiimonas arsenicoxydans] Length = 526 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 92/297 (30%), Positives = 147/297 (49%), Gaps = 12/297 (4%) Query: 74 IAIPFSLPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSD 133 I L + + R ++P G + S++ GD VK G VL I S L+ S+ Sbjct: 210 IKTNLQLIGEIRLNEDRTVHVVPRLAGIVDSVRANAGDQVKKGQVLAVISSQSLSDQRSE 269 Query: 134 ERKAQSTLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAARAHARVAALGGTA 193 AQ L+L R ER++SL + ISA +D QA++ ++A A A ++AALG + Sbjct: 270 LLAAQKRLSLARTTYEREKSLWEEKISAEQDYLQARAAMQEAQIMAQSAQQKLAALGASP 329 Query: 194 ASGGSARLAVRAPVSGHVIELNAAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLH 253 +S + +R+P+ G + + N + GS ++ A++ V+DLS+V+V + + +D+ L Sbjct: 330 SSSNLTQYEIRSPIDGVITDKNISTGSVTKED-ANIFVVSDLSTVWVDLTVRAEDIGALK 388 Query: 254 IGQDAEIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQATLRE 313 +GQ + A+ GQ + A V +G ++ TRT K RI++ N +PG+ L Sbjct: 389 VGQKGTVKSSAFDGQDVGA-VSYIGALVGEQTRTAKARIVLANPKGLWRPGLPVNVNLV- 446 Query: 314 QAHAGLL-VPMTAVVQS----GFANRVFVEVAPWKFEARKIMLGAQVGNDVEVTDGL 365 AG + VP+ V S G VF EAR + LG G +EV GL Sbjct: 447 ---AGEVDVPVAISVDSIQTLGDREVVFGRYGNL-LEARPLELGRSDGKLIEVLSGL 499 >gi|134094811|ref|YP_001099886.1| cobalt-zinc-cadmium resistance protein CzcB [Herminiimonas arsenicoxydans] Length = 524 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 86/291 (29%), Positives = 148/291 (50%), Gaps = 12/291 (4%) Query: 94 ILPPTTGRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSDERKAQSTLALTRRNLERQRS 153 ++P G + S++ GD VK G VL I S L+ S+ AQ L+L R ER+++ Sbjct: 228 VVPRLAGIVESVRANAGDQVKKGQVLAVISSQSLSDQRSELLAAQKRLSLARTTYEREKT 287 Query: 154 LDQANISARRDLEQAQSDYEQADSEAARAHARVAALGGTAASGGSARLAVRAPVSGHVIE 213 L + ISA +D QA++ ++A A A ++AALG +++S + +R+P++G + + Sbjct: 288 LWEEKISAEQDYLQARAVMQEAQIMAQSAQQKLAALGASSSSSNLTQYEIRSPINGVITD 347 Query: 214 LNAAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDAEIALDAYPGQPLQAK 273 + G+ ++ ++ ++DLS+V+V + + +D+ L GQ + +A+ Q + Sbjct: 348 KRISTGTVAKEDD-NIFVISDLSTVWVDLTVRAEDIGALKTGQKGTVKSNAFDAQDV-GT 405 Query: 274 VKSVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQATLREQAHAGLL-VPMT----AVVQ 328 V +G ++ TRT K RI++ N +PG+ L AG + VP+T +V + Sbjct: 406 VSYIGALVGEQTRTAKARIVLANPKGIWRPGLPVNVNLV----AGEVDVPVTISTDSVQK 461 Query: 329 SGFANRVFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAGTRVVVKDGVLL 379 G + VF EAR I LG G VEV GL A + + L+ Sbjct: 462 MGDRDVVFGRYGNL-LEARPIDLGRTDGKLVEVLGGLLADEKYAGTNSFLI 511 >gi|134096397|ref|YP_001101472.1| putative heavy metal efflux system protein [Herminiimonas arsenicoxydans] Length = 470 Score = 77.0 bits (188), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 77/287 (26%), Positives = 128/287 (44%), Gaps = 34/287 (11%) Query: 109 LGDAVKAGDVLFEIESSDLAQAVSDERKAQSTLALTRRNL--ERQRSLDQANISARRDLE 166 +G V+ G VL+EI S +L Q R+ + LTRR+ E+ R +D +A Sbjct: 153 VGQQVRKGQVLYEIYSPELQQ-----RQREYVDLLTRRDAMQEKSRDMDMLGPNAAMMGS 207 Query: 167 QAQSDYEQADS-----------EAARAHARVAALGGTAASGGSARLAVRAPVSGHVIELN 215 A+ + D E RV + + +RA SG V ++ Sbjct: 208 LAKERFRTRDRLLAADIPPEELELLEKGRRVPEV-----------MPIRATQSGIVTAIS 256 Query: 216 AAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDAEIALDAYPGQPLQ--AK 273 A GS+ N ++M +D S V+ + ++ L G EI L + +Q A+ Sbjct: 257 AREGSYINP-MQTLMVYSDNSRVWAEVTLFPDQISWLRNGD--EIVLTSGLDNTVQHTAR 313 Query: 274 VKSVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQATLREQAHAGLLVPMTAVVQSGFAN 333 + +DP +RT K+R+ ++ E PG +A+AT++ AH + VP AV+++G Sbjct: 314 INLATLQIDPASRTAKLRLPLSKAGEAFLPGAYAKATIKSAAHRAVSVPRDAVIRTGHGE 373 Query: 334 RVFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAGTRVVVKDGVLLN 380 V V F + + +G + VE+ GL+AG R+ V LL+ Sbjct: 374 FVVVSEGDGHFRSAAVAIGLEDERMVEIRSGLKAGDRIAVNGQFLLD 420 >gi|134094274|ref|YP_001099349.1| hypothetical protein HEAR1042 [Herminiimonas arsenicoxydans] Length = 382 Score = 62.0 bits (149), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 88/327 (26%), Positives = 147/327 (44%), Gaps = 32/327 (9%) Query: 64 LKTALIEQRSIAIPFSLPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIE 123 +KTAL EQ++I+I V A + P + +I R G VKAG +LF + Sbjct: 41 VKTALAEQKTISIKAFANGYVTA--INTVDVRPKVQNIVRTIHVREGQFVKAGQLLFTL- 97 Query: 124 SSDLAQAVSDERKAQSTLALTRRNLER-QRSLDQANISARRDLEQAQSDY-EQADSEAAR 181 DER QS +A + L Q L A ++ +R+ + ++ QA + AR Sbjct: 98 ---------DERSDQSNVAKAQAELAAGQSDLADAELALKRNEDLLSRNFVSQAVVDTAR 148 Query: 182 AHARVAALGGTA------ASGGSARLA---VRAPVSGHVIELNAAVGSFWNDNTASVMTV 232 +VAAL T+ A + L+ + A +SG + ++ GS + ++T+ Sbjct: 149 --NKVAALRNTSKANRAIAESSNITLSDNRIAASISGRIGIISVHPGSLAQPSGDPMVTI 206 Query: 233 ADLSSVYVTASAQEKDLARL--HIGQDAEIALDAYPGQP-LQAKVKSVGQVLDPDTRTVK 289 + L + V+ + E++LA + Q PGQP + K+ + D + T++ Sbjct: 207 SQLDPIAVSFTLPERELANITATYPQGNAPVTARLPGQPEMTGKLIFIDNAADTQSGTIR 266 Query: 290 VRILVTNTDERLKPGMFAQATL--REQAHAGLLVPMTAVVQSGFANRVFVEVAPWKFEAR 347 ++ N ++L PG F +L R +HA +LVP AVV ++V + + Sbjct: 267 MKAQFDNAAQKLWPGSFVNVSLVSRNVSHA-VLVPAQAVVTGPIDKFIYVVQPDATVKEQ 325 Query: 348 KIMLGAQVGNDVEVTDGLQAGTRVVVK 374 K+ + A V GL AG RVVV+ Sbjct: 326 KVDVVAIEEGQAAVI-GLDAGARVVVE 351 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.316 0.130 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 575,987 Number of extensions: 20481 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 12 Length of query: 381 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 298 Effective length of database: 726,862 Effective search space: 216604876 Effective search space used: 216604876 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)