BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2071.p (534 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094609|ref|YP_001099684.1| L-aspartate oxidase [Herminiimo... 853 0.0 gi|134094982|ref|YP_001100057.1| succinate dehydrogenase, cataly... 234 7e-63 gi|134096484|ref|YP_001101559.1| fumarate reductase, or succinat... 206 2e-54 >gi|134094609|ref|YP_001099684.1| L-aspartate oxidase [Herminiimonas arsenicoxydans] Length = 534 Score = 853 bits (2204), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/536 (76%), Positives = 468/536 (87%), Gaps = 5/536 (0%) Query: 1 MKCDVAILGSGLAGLSVALHLAEKYQVVVVSKGELLDGASNWAQGGIAAVLDSSDSHAQH 60 MK DVAI+GSGLAGL+VALHLAE +V ++SK LLDGASNWAQGGIAAVLDS+DS +H Sbjct: 1 MKFDVAIVGSGLAGLTVALHLAETRKVTIISKRTLLDGASNWAQGGIAAVLDSNDSVDEH 60 Query: 61 IDDTLVAGAGLCDESATRTIVEHGRQAIEWLIAQGVPFTRDDSAELGYHLTREGGHSQRR 120 + DTLVAG GLCDE ATR I+EHGR+AIEWLI QGVPFT+D SAELG+HLTREGGH QRR Sbjct: 61 VADTLVAGGGLCDEPATRFILEHGREAIEWLIEQGVPFTQDASAELGFHLTREGGHGQRR 120 Query: 121 IIHAADATGHAVQVTLEQRVRAHPNITLLEHHCAIDLIHARKLDPK-AEPRCLGVHVQDT 179 IIHAADATGHAVQVTLE++VR HPNITL+EHH AID+I + L+ K PRCLG++VQD Sbjct: 121 IIHAADATGHAVQVTLEEKVRNHPNITLMEHHYAIDVITSDVLEKKKGVPRCLGLYVQDV 180 Query: 180 ASGRVIAIAAARTVLATGGAGKVYLYTSNPDSATGDGIAMAWRAGCRVANMEFIQFHPTC 239 +G+VI IAA TVLATGGAGKVYLYT+NPD+ATGDGIAMAWRAGCR+ANMEFIQFHPTC Sbjct: 181 KTGKVITIAAEHTVLATGGAGKVYLYTTNPDTATGDGIAMAWRAGCRIANMEFIQFHPTC 240 Query: 240 LYHPYAKSFLISEALRGEGALLKLPASAGAAAGQRFMPEHDERCELAPRDIVARAIDFEM 299 LYHPYAKSFLI+EA+RGEG +LKLP +AG G+RFMP HDER ELAPRDIVARAID EM Sbjct: 241 LYHPYAKSFLITEAVRGEGGVLKLPVTAGKNRGKRFMPSHDERAELAPRDIVARAIDAEM 300 Query: 300 KKRGLDHVDLDISHQSPAFIAEHFPTIQARCLEFGIDITRQPIPVVPAAHYTCGGVVTDM 359 KKRG+D+VDLDIS +SP F+ EHFPTI ARCLE GID+T+ PIPVVPAAHYTCGGV+TD+ Sbjct: 301 KKRGIDYVDLDISDKSPEFLKEHFPTIMARCLELGIDMTKGPIPVVPAAHYTCGGVITDL 360 Query: 360 AGRTDLPGLYAVGETAYTGLHGANRLASNSLLECLVLGRSAAADIEQELAAGTWRVTEVP 419 +GRTDLPGLYAVGE TGLHGANRLASNSLLECLVLGRS A I+ + + +P Sbjct: 361 SGRTDLPGLYAVGEATCTGLHGANRLASNSLLECLVLGRSTAQYIDTQPKQ---KAVSLP 417 Query: 420 QWDESRVSDADEEVVIAHNWDELRRFMWNYVSIVRTDKRLERAQHRIRLLKEEIDEYYAN 479 WDESRV+DADEEV+IA+NWDELRRFMWNYV IVRT KRLERAQHRIRLLKEEIDEYYAN Sbjct: 418 AWDESRVTDADEEVIIANNWDELRRFMWNYVGIVRTTKRLERAQHRIRLLKEEIDEYYAN 477 Query: 480 FRISRDLLELRNLVDVAQLIVSSALTRKESRGLHFSQDYPRTLPRATPTIL-PGRQ 534 FRI+ DLLELRNLV+VA LIV+SAL+R+ESRGLH+S+DYP TLP+A P++L P R+ Sbjct: 478 FRITPDLLELRNLVEVASLIVNSALSRRESRGLHYSRDYPDTLPKALPSVLSPDRR 533 >gi|134094982|ref|YP_001100057.1| succinate dehydrogenase, catalytic and NAD/flavoprotein subunit [Herminiimonas arsenicoxydans] Length = 592 Score = 234 bits (598), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 187/558 (33%), Positives = 278/558 (49%), Gaps = 65/558 (11%) Query: 4 DVAILGSGLAGLSVALHLAEK-YQVVVVSKGELLDGASNWAQGGIAAVLDS--SDSHAQH 60 D I+G+G +G+ +L LAE V V+SK + AQGGI A L + D+ H Sbjct: 14 DAIIIGAGGSGMRASLQLAEAGLNVAVLSKVFPTRSHTVAAQGGIGASLGNMAEDNWYWH 73 Query: 61 IDDTLVAGAGLCDESATRTIVEHGRQAIEWLIAQGVPFTRDDSAEL-----GYHLTREGG 115 + DT+ L D+ A + + + L G+PF R+ + G H G Sbjct: 74 MFDTIKGSDYLGDQDAIEFMCREAPKVVYELEHFGMPFDRNADGTIYQRPFGGHTANFGE 133 Query: 116 HSQRRIIHAADATGHAVQVTLEQR-VRAHPNITLLEHHCAIDLIHARKLDPKAEPRCLGV 174 +R AAD TGHA+ TL QR VRA + + AIDL+ A +GV Sbjct: 134 KPVQRACAAADRTGHALLHTLYQRNVRARTHFFV--EWMAIDLMR------DAAGDVIGV 185 Query: 175 HVQDTASGRVIAIAAARTVLATGGAGKVYLYTSNPDSATGDGIAMAWRAGCRVANMEFIQ 234 + +G V+ + A T+ ATGGAG+++ ++N TGDG+ MA RAG + +MEF Q Sbjct: 186 VALEMETGEVMMLEAKTTMFATGGAGRIFAASTNAFINTGDGLGMAARAGLPLEDMEFWQ 245 Query: 235 FHPTCLYHPYAKSFLISEALRGEGALLKLPASAGAAAGQRFMPEHDERC-ELAPRDIVAR 293 FHPT + LI+E +RGEG +L + G+RFM + +LAPRD V+R Sbjct: 246 FHPTGV---AGAGVLITEGVRGEGGILV------NSQGERFMERYAPTLKDLAPRDFVSR 296 Query: 294 AIDFEMKK-RGL----DHVDLDISHQSPAFIAEHFPTIQARCLEF-GIDITRQPIPVVPA 347 ++D E+K+ RG D+V LD+ H I + P+I +F +D TR+ IPVVP Sbjct: 297 SMDQEIKEGRGCGPNKDYVMLDLRHIGADTIQKRLPSILEIAHKFANVDATREAIPVVPT 356 Query: 348 AHYTCGGVVTDMAGRTDLP----------GLYAVGETAYTGLHGANRLASNSLLECLVLG 397 HY GG+ T++ G+ P GLYA+GE A +HGANRL +NSLL+ +V G Sbjct: 357 IHYQMGGIPTNIHGQVVEPKDGNPNHVVNGLYAIGECACVSVHGANRLGTNSLLDLIVFG 416 Query: 398 RSAAADIEQE-----------LAAGTWRVTEVPQWDESRVSDADEEVVIAHNWDELRRFM 446 R+A I + A ++ + + S + + V +++R M Sbjct: 417 RAAGNHILKSNLKQREHKPLPADAADLALSRLAHLENSTGGERVQNVA-----NDIRSTM 471 Query: 447 WNYVSIVRTDKRLERAQHRIRLLKE-----EIDEYYANFRISR-DLLELRNLVDVAQLIV 500 Y + RTD+ L+ +I L E + F +R + LEL N+++VA+ + Sbjct: 472 QQYCGVFRTDELLQTGYKKIMELDERRKHVSFKDKSRVFNTARVEALELDNMIEVAKATI 531 Query: 501 SSALTRKESRGLHFSQDY 518 +SA RKESRG H +DY Sbjct: 532 TSAAARKESRGAHAHRDY 549 >gi|134096484|ref|YP_001101559.1| fumarate reductase, or succinate dehydrogenase, flavoprotein subunit [Herminiimonas arsenicoxydans] Length = 580 Score = 206 bits (525), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 178/553 (32%), Positives = 259/553 (46%), Gaps = 53/553 (9%) Query: 4 DVAILGSGLAGLSVALHLAE---KYQVVVVSKGEL-LDGASNWAQGGIAAVLDSSDSHAQ 59 D+ +LGSG AGL ALH + + + V KG L G + QGG L DS + Sbjct: 13 DILVLGSGGAGLFAALHAHQANPELSITVAVKGLLGKSGCTRMVQGGYNVALAEGDSIER 72 Query: 60 HIDDTLVAGAGLCDESATRTIVEHGRQAIEWLIAQ-GVPFTRDDSAELGYHLTREGGHSQ 118 H DT+ G L D+ T+V + I L + G F R+ + H G + Sbjct: 73 HFMDTIEGGKWLSDQDLAWTLVTKAIERIHELENELGCFFDRNPDGTV--HQKAFAGQTF 130 Query: 119 RRIIHAADATGHAVQVTLEQRVRAHPNITLLEHHCAIDLIHARKLDPKAEPRCLGVHVQD 178 R +H D TG + L +++ H I LE H A++LI + D K+ GV + D Sbjct: 131 DRTVHKGDLTGIEIMNRLAEQIW-HRGIARLEEHRAVELI--KTADGKS---LAGVLMID 184 Query: 179 TASGRVIAIAAARTVLATGGAGKVYLYTSNPDSATGDGIAMAWRAGCRVANMEFIQFHPT 238 G + + A +LATGG +Y + + + DG+AMA RAG + +ME +QFHPT Sbjct: 185 MRCGEFVFVQAKAVLLATGGGPTMYKFHTPSGEKSCDGLAMALRAGLPLRDMEMVQFHPT 244 Query: 239 CLY---HPYAKSFLISEALRGEGALLKLPASAGAAAGQRFMPEHDERCELAPRDIVARAI 295 L ++ E LRG G L +RFM +D R E A RDIV+R+I Sbjct: 245 GLLAGPDTRMTGTVLEEGLRGAGGYLL------NGLKERFMQNYDPRKERATRDIVSRSI 298 Query: 296 DFEMKK-RGLDH--VDLDISHQSPAFIAEHFPTIQARCLEFGIDITRQPIPVVPAAHYTC 352 EM+ R + V + ++H P + F + RC + G D+ + VVP AHY Sbjct: 299 YSEMRAGRTTPNGGVYIQMAHLGPEKVRVQFKGMVERCADCGFDLAADLVEVVPTAHYMM 358 Query: 353 GGVVTDMAGRTDLPGLYAVGETAYTGLHGANRLASNSLLECLVLGRSAAADIEQELAAGT 412 GGV+ T L GL+A GE G+HGANRL N + V G A + +AA Sbjct: 359 GGVIFRPDCTTALHGLFAAGEDT-GGVHGANRLGGNGVANSTVFGGIAG----ESMAAWL 413 Query: 413 WRVTEVPQWDESRVSDADEEVVIAH---------NWDELR----RFMWNYVSIVRTDKRL 459 E+ + D + + E + AH N LR MW V I+RT + L Sbjct: 414 LNNNELREPDIAAI----ELSITAHRAPFGNPIGNLQALREALYECMWEDVGILRTAEGL 469 Query: 460 ERAQHRIRLLKEEI------DEYYANFRISRDLLELRNLVDVAQLIVSSALTRKESRGLH 513 R ++ L E+ D+ A D + LR+L+ V+++I ++AL R++SRG H Sbjct: 470 RRGIAKLAELDRELLTIGVADDDPAFNMTWHDWMNLRSLLAVSRVIATAALAREDSRGAH 529 Query: 514 FSQDYPRTLPRAT 526 F +D P+T AT Sbjct: 530 FREDAPQTSALAT 542 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.321 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,028,360 Number of extensions: 43238 Number of successful extensions: 132 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 3 Length of query: 534 Length of database: 1,000,347 Length adjustment: 86 Effective length of query: 448 Effective length of database: 716,977 Effective search space: 321205696 Effective search space used: 321205696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)