BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1982.p (754 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095317|ref|YP_001100392.1| bifunctional (p)ppGpp synthetas... 1165 0.0 gi|134095007|ref|YP_001100082.1| (p)ppGpp synthetase I [Herminii... 357 1e-99 >gi|134095317|ref|YP_001100392.1| bifunctional (p)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Herminiimonas arsenicoxydans] Length = 760 Score = 1165 bits (3013), Expect = 0.0, Method: Compositional matrix adjust. Identities = 567/760 (74%), Positives = 638/760 (83%), Gaps = 8/760 (1%) Query: 1 MSLTTTDSTLSAAASSK----RAAGSGRTATPQEVAPAAAHSGVATFAHLIPKLEEYLTP 56 MSLT DSTLS A +++ R+A T PA A GVA+ L KL EYLTP Sbjct: 1 MSLTPADSTLSVAPNNRAVPDRSASKVTTNKSPASTPAPA-PGVASVTQLTNKLAEYLTP 59 Query: 57 SELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMED 116 SELKK+KEAYRF+DE HLGQMR SGEPYISHP+AVAEICA+WKLDAQA+MAA LHDVMED Sbjct: 60 SELKKIKEAYRFSDEAHLGQMRMSGEPYISHPIAVAEICADWKLDAQAIMAALLHDVMED 119 Query: 117 QGVKKEELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKL 176 Q V+K+ELIERFGA VA++VDGLSKL+K+EFQSQ+EAQAENFRKMLLAMARDVRVIL+KL Sbjct: 120 QDVRKDELIERFGAPVANIVDGLSKLEKLEFQSQIEAQAENFRKMLLAMARDVRVILIKL 179 Query: 177 ADRLHNMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSFSHLYPLRHRT 236 ADRLHNMRTLGSM KKRRI+RETMEVYVPIAHRLGLNNIYRELQ+L+FSHLYP+R++T Sbjct: 180 ADRLHNMRTLGSMSAVKKRRIARETMEVYVPIAHRLGLNNIYRELQDLAFSHLYPMRYKT 239 Query: 237 LSKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQV 296 L KAVKAARGNRREVV+KI+ESV TL A GIPAQV GREKTLYGIY KM NK LSFSQV Sbjct: 240 LGKAVKAARGNRREVVSKILESVNKTLEATGIPAQVYGREKTLYGIYTKMLNKRLSFSQV 299 Query: 297 LDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTP 356 LD+YGFRVVVDSFANCYVALGTLH+LFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTP Sbjct: 300 LDIYGFRVVVDSFANCYVALGTLHALFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTP 359 Query: 357 VEFQIRTTEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLE 416 VEFQIRT +MHRVAESGVAAHWLYKDDEGSL DLQQRTHAWLQSLLDIQKQTGDSAEFLE Sbjct: 360 VEFQIRTQDMHRVAESGVAAHWLYKDDEGSLNDLQQRTHAWLQSLLDIQKQTGDSAEFLE 419 Query: 417 HVKVDLFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTSVIINHEPAPLRSEL 476 HVKVDLFPDSVYVFTPKSKIIALPRGATALDFAY+IHTD+GDQT +V IN E PLR+EL Sbjct: 420 HVKVDLFPDSVYVFTPKSKIIALPRGATALDFAYTIHTDVGDQTIAVKINLEQVPLRTEL 479 Query: 477 HNGDIVEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTANRVESQTVGRRLLGNALHTL 536 NGDIVEIITSP+SRP+PNWLG+VRTGKARSAIRH LRT N VES +GR+LLG AL L Sbjct: 480 RNGDIVEIITSPSSRPNPNWLGFVRTGKARSAIRHHLRTINLVESIDLGRKLLGQALSAL 539 Query: 537 GIEPELPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMM--DTGVSVPQLD 594 + P+L + ERLLNESSAK++DDLYA+IG+G RMA LVARHI+ M+ DT P LD Sbjct: 540 NLNPDLSPAVTERLLNESSAKSLDDLYADIGVGKRMAALVARHILGMLENDTPSPHPLLD 599 Query: 595 PEGHMLPNKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLKPDQTLIVHTQECETAKRL 654 G ++ + DPVVI GSEG + QL+ CC PIPGD + G +K DQ L +HT EC+ A+R Sbjct: 600 VNGEVVNKQIDPVVIYGSEGVAVQLAPCCQPIPGDAIIGQMKRDQGLTIHTLECDVARRQ 659 Query: 655 HEKEPDRWIELVWGHDLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGG 714 KEPDRWI + WG +NRRFDC IT+L NE+G LAR+AAEIGESDANI V MD++ Sbjct: 660 RSKEPDRWIGVTWGAGINRRFDCGITLLARNEKGILARVAAEIGESDANITHVGMDEDKE 719 Query: 715 NASMKYLRFTIQIEDRVHLARLMRGIRKIDSVARILRERG 754 + + YL FT+Q+EDRVHLARLMR +R I V R+LR +G Sbjct: 720 HM-ITYLHFTVQVEDRVHLARLMRNLRAIPGVTRLLRHKG 758 >gi|134095007|ref|YP_001100082.1| (p)ppGpp synthetase I [Herminiimonas arsenicoxydans] Length = 761 Score = 357 bits (916), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 230/750 (30%), Positives = 373/750 (49%), Gaps = 62/750 (8%) Query: 50 LEEYLTPSELKKVKEAYRFADEMHLGQMRKSGEPYIS-------HPLAVAEICAEWKLDA 102 L LT + +V +A + + G+ +G+ VA + +E + DA Sbjct: 15 LRSGLTEHDSARVMDALAYVKPFYTGKSVSAGQDAFESEQDAFEFAQGVATVLSELETDA 74 Query: 103 QAMMAAFLHDVMEDQGVKKEELIERFGASVASLVDG---LSKLDKIEFQSQVEAQAEN-- 157 +AA L ++ E++ ERFG V+ LV G L +L + F A+ +N Sbjct: 75 DTRIAALLFELEMIAPAAAEKIEERFGKDVSDLVAGVRQLMRLHEATFTQHEAARNKNAA 134 Query: 158 ---------FRKMLLAMARDVRVILVKLADRLHNMRTLGS--MPPEKKRRISRETMEVYV 206 RKMLLAMA D+RV+LV+L R+ +R + E+ R+ +RET ++Y Sbjct: 135 QEAAAQLEVLRKMLLAMASDMRVVLVRLGTRVTTLRYFADNKLVNERSRQYARETFDLYA 194 Query: 207 PIAHRLGLNNIYRELQELSFSHLYPLRHRTLSKAVKAARGNRREVVTKIMESVTSTLIAA 266 P+A+RLG+ + EL++LSF L P +R ++ ++ R R V ++ + S + AA Sbjct: 195 PLANRLGIWQLKWELEDLSFRFLEPEAYRRIASLLEEKRVEREAFVENSIKRLKSEMAAA 254 Query: 267 GIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPM 326 GI A+V GR K ++ I+ K+R K + FS + DV FRV+VD CY LG +H+++ P+ Sbjct: 255 GIKAEVFGRPKHIFSIWSKLRGKEIEFSDLYDVRAFRVIVDDVKTCYTVLGIVHNIWTPI 314 Query: 327 PGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQIRTTEMHRVAESGVAAHWLYKDDEGS 386 P +F DYI+ PK NGYQSLHT +I G P+E Q+RT +MH AE GVAAHW YK+ GS Sbjct: 315 PEEFDDYISRPKANGYQSLHTIVIAEDGRPLEVQVRTNDMHDFAEYGVAAHWRYKESGGS 374 Query: 387 LTDLQQRTH--AWLQSLLDIQKQTGDSA--------EFLEHVKVDLFPDSVYVFTPKSKI 436 Q+ AWL+ LL + + D+ +++E +K D +YV TP++++ Sbjct: 375 NFAGQKYDEKIAWLRQLLAWKSEVVDTVVGQEDVHRDWVEKLKSATLDDRIYVLTPQARV 434 Query: 437 IALPRGATALDFAYSIHTDIGDQTTSVIINHEPAPLRSELHNGDIVEIITSP-------T 489 I LP GAT +DFAY +H+D+G + ++ PL + L NG V+IIT+ T Sbjct: 435 IELPNGATPIDFAYHLHSDVGHRCRGARVDGVLVPLNTVLKNGQTVDIITAKGASAVTGT 494 Query: 490 SRPSPNWL--GYVRTGKARSAIRHRLRTANRVESQTVGRRLLGNALHTLGIEPELPSHIV 547 PS +WL GY + + RS +R ++ E+ GR L+ L G + + Sbjct: 495 VGPSRDWLSPGYAASPRTRSKVRAWFNAIDQQETLASGRALIEKTLQREG----KTAVNL 550 Query: 548 ERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHMLPNKP--- 604 E L ++ +DDL+ +G V + + D + Q + E ++P K Sbjct: 551 EELAHKLDFAKLDDLFRAVGKDEFSLRTVEQALRG--DEASAAAQAELEDVVIPRKSRAS 608 Query: 605 -------DPVVITGSEGASAQLSSCCMPIPGDRLTGYLKPDQTLIVHTQECETAKRLHEK 657 V++ G++G QL+ CC P P D + G++ + + +H +C+ + K Sbjct: 609 SVMHGAKSGVLVVGTDGLMTQLAKCCKPAPPDEIVGFVTRGKGVSIHRADCKNFVEMRHK 668 Query: 658 EPDRWIELVWGHDLNRR-FDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNA 716 P+R ++ WG + + I +L + +G L I+ N++ V+ G A Sbjct: 669 APERVVQTAWGKTESATVYPVDIFVLASDRQGLLRDISDIFLREKINVIGVSTQSVKGQA 728 Query: 717 SMKYLRFTIQIEDRVHLARLMRGIRKIDSV 746 M FT +I L + + I ++ V Sbjct: 729 RMA---FTAEIASTAQLQKALAVIGEVKGV 755 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,373,223 Number of extensions: 57007 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 2 Length of query: 754 Length of database: 1,000,347 Length adjustment: 89 Effective length of query: 665 Effective length of database: 707,092 Effective search space: 470216180 Effective search space used: 470216180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)