BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1945.p (409 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|161485636|ref|YP_001100411.2| aminotransferase AlaT [Herminii... 754 0.0 gi|134094271|ref|YP_001099346.1| putative aspartate aminotransfe... 99 3e-22 gi|134094550|ref|YP_001099625.1| putative aminotransferase [Herm... 83 2e-17 gi|134095746|ref|YP_001100821.1| histidinol-phosphate aminotrans... 57 1e-09 >gi|161485636|ref|YP_001100411.2| aminotransferase AlaT [Herminiimonas arsenicoxydans] Length = 407 Score = 754 bits (1948), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/407 (86%), Positives = 387/407 (95%) Query: 1 VRPIQKSKKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR 60 +RPIQKSKKLADVCYDIRGPVLEKARQMEE+GHKIIKLNIGNLA FGFDAPDEIVQDMIR Sbjct: 1 MRPIQKSKKLADVCYDIRGPVLEKARQMEEDGHKIIKLNIGNLATFGFDAPDEIVQDMIR 60 Query: 61 NMGNASGYTDSKGLFAPRKSVMHYTQQKKIEGVTIDDVYLGNGASELIVMSVNALLNTGD 120 N+ NASGYTDSKGLFAPRK++M Y+Q+KKI GVTIDD+YLGNGASELIVMS+NALLNTGD Sbjct: 61 NLSNASGYTDSKGLFAPRKAIMQYSQEKKIAGVTIDDIYLGNGASELIVMSMNALLNTGD 120 Query: 121 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNP 180 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQ W PDI DI+KKI NTKAIVVINPNNP Sbjct: 121 EVLVPSPDYPLWTAAVSLSGGTPVHYVCDEQADWFPDIDDIRKKINSNTKAIVVINPNNP 180 Query: 181 TGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSK 240 TGALY VE+L++I++LAR+HQLI+ ADEIYDKVLYDG H S+ASLADDVLFITFNGLSK Sbjct: 181 TGALYPVELLQQIVDLAREHQLIVFADEIYDKVLYDGAKHDSIASLADDVLFITFNGLSK 240 Query: 241 NYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLV 300 NYR+CGYRAGWM+VSGEK++A+DYIEGLNMLASMRLCANAPGQ+AIQTALGGYQSINDLV Sbjct: 241 NYRACGYRAGWMIVSGEKRYAKDYIEGLNMLASMRLCANAPGQYAIQTALGGYQSINDLV 300 Query: 301 APNGRLTKQRDLAHKLLTDIPGVTCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEK 360 P GRLT+QRDLAHKLLT+IPGVTCVKPKSALYMFPRLDP++YPIKDDQ+FAY+LLAEE Sbjct: 301 GPGGRLTRQRDLAHKLLTEIPGVTCVKPKSALYMFPRLDPKMYPIKDDQDFAYQLLAEES 360 Query: 361 VLIVQGTGFNCPTPDHFRVVFLPNTDDLSESMGRIAHFLEGYRRRHG 407 VLIVQGTGFNCPTPDHFRVVFLPN+DDL+ ++GRIAHFLEGYR+R G Sbjct: 361 VLIVQGTGFNCPTPDHFRVVFLPNSDDLTIAIGRIAHFLEGYRKRLG 407 >gi|134094271|ref|YP_001099346.1| putative aspartate aminotransferase A [Herminiimonas arsenicoxydans] Length = 368 Score = 99.4 bits (246), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 97/381 (25%), Positives = 169/381 (44%), Gaps = 21/381 (5%) Query: 25 ARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVM-H 83 A ++E +G II + IG F G YT + GL R ++ H Sbjct: 2 AAELERQGRHIIHMGIGE-PDFTAAPSVLAAAAQAMADGRMQ-YTSATGLPELRAAIAAH 59 Query: 84 YTQQKKIEGVTIDDVYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTP 143 Y ++ V + + GAS ++++ AL+ G EVL+ P YP V+ G+ Sbjct: 60 YRDMYGVD-VAPSRIIITAGASGALLLACAALVEKGAEVLMSDPSYPCNRHFVAAFDGSA 118 Query: 144 VHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLI 203 + +Q +++ T+ +++ +P+NPTG + L++I+E+ R Q Sbjct: 119 KMIASGPEHRFQLSADMVQQNWGEQTRGVLLASPSNPTGTSIDEDELRKIVEVVRGKQGF 178 Query: 204 ILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARD 263 + DEIY + YD ++L SL DDV+ I N SK + G+R GW+VV Sbjct: 179 TIVDEIYHGLRYDAAPFSAL-SLGDDVIVI--NSFSKYFNMTGWRLGWLVVP------ET 229 Query: 264 YIEGLNMLA-SMRLCANAPGQFAIQTALGGYQSINDLVAPNGRLTKQRDLAHKLLTDIPG 322 + + LA ++ +CA++ Q A A ++ A ++RD L ++ Sbjct: 230 LVPPIEKLAQNLFICASSLAQHA-GLACFAPAAMTVYEARKAEFKRRRDYIVPALEELGF 288 Query: 323 VTCVKPKSALYMFPRLDPEIYPIKDD-QEFAYELLAEEKVLIVQGTGFNCPTPDHF-RVV 380 V P A Y++ + + DD E +L E V++V G F T + R+ Sbjct: 289 KVPVMPDGAFYVY----ADCSALSDDADELTIAMLNEAGVVMVPGLDFGPSTAHRYVRIS 344 Query: 381 FLPNTDDLSESMGRIAHFLEG 401 + + ++L E++ R+ FL G Sbjct: 345 YATSMENLQEAIARLRIFLSG 365 >gi|134094550|ref|YP_001099625.1| putative aminotransferase [Herminiimonas arsenicoxydans] Length = 312 Score = 83.2 bits (204), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 71/315 (22%), Positives = 142/315 (45%), Gaps = 30/315 (9%) Query: 102 NGASELIVMSVNALLNTGDEVLV--PSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIA 159 NG+ E + +++ LV P+P Y ++ A L+G P CD + + PD A Sbjct: 5 NGSREALFALAQTVIDPSQHALVVCPNPLYQIYEGAAYLAGAQPYFVNCDPARNFAPDFA 64 Query: 160 DIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNT 219 I ++ + + V +P NP GA+ +++ E+ L+ ++ +I ADE Y ++ + Sbjct: 65 AITHEVWSRVQLVYVCSPGNPAGAVLTLDDWAELFALSDRYNFVIAADECYSEIYFKAEA 124 Query: 220 HTSLASLADDV------LFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEGLNMLAS 273 A + I F+ LSK G R+G+ V+G+ + + ++ + + Sbjct: 125 PLGGLEAAHKLGRKGYPRLIGFSSLSKRSNVPGMRSGF--VAGDAEILKKFL----LYRT 178 Query: 274 MRLCANAPGQFAIQTA-LGGYQSINDLVAPNGRLTKQRDLAHKLLTDIPGVTCVKPKSAL 332 A +P ++Q+A + + ++ + ++ + LL ++ V+ P + Sbjct: 179 YHGSAMSP---SVQSASIAAWNDEQHVIDNREKYIQKFNQVTPLLQEVMDVSL--PDAGF 233 Query: 333 YMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT-------GFNCPTPDHFRVVFLPNT 385 Y++ ++D ++ I D EFA L AE V ++ G+ G N P + R+ + Sbjct: 234 YLWAKVD-KLAAI-GDIEFAKRLYAEYNVTVLPGSYLGREAHGIN-PGQNRIRMALVAEV 290 Query: 386 DDLSESMGRIAHFLE 400 D+ E+ RI F + Sbjct: 291 DECLEAAQRIVEFTQ 305 >gi|134095746|ref|YP_001100821.1| histidinol-phosphate aminotransferase [Herminiimonas arsenicoxydans] Length = 368 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 53/234 (22%), Positives = 101/234 (43%), Gaps = 14/234 (5%) Query: 20 PVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRK 79 P+ E AR+ + +I+KL N G P+ + + + +A Y D G Sbjct: 21 PIAEVAREFGLDEARIVKL-ASNENPLGM--PESAKKAIALAIADAGRYPDGNGFELKAA 77 Query: 80 SVMHYTQQKKIEGVTIDDVYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLS 139 Y GV + + LGNG+++++ +S +A + G V+ + ++ A Sbjct: 78 LTAKY-------GVPAEWITLGNGSNDILELSAHAFVQPGQSVIYAQYSFAVYPLATQAV 130 Query: 140 GGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQ 199 GG + + + D+ + I +T+ I + NPNNPTG ++ ++ Sbjct: 131 GGRSLVVAAKD---FGHDLEAMIDAIEDDTRLIFIANPNNPTGTFIPGHEIESFLKRVPP 187 Query: 200 HQLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMV 253 + +++L DE Y + L + S+A +A + +SK Y G R G+ + Sbjct: 188 NVVVVL-DEAYTEFLAPELQYDSIAWVAQYPNLLVSRSMSKVYGLAGLRIGYGI 240 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 809,040 Number of extensions: 33406 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 5 Length of query: 409 Length of database: 1,000,347 Length adjustment: 84 Effective length of query: 325 Effective length of database: 723,567 Effective search space: 235159275 Effective search space used: 235159275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)