BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1691.p (662 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094035|ref|YP_001099110.1| DNA topoisomerase IV subunit B ... 1200 0.0 gi|134093298|ref|YP_001098373.1| DNA gyrase subunit B [Herminiim... 311 6e-86 >gi|134094035|ref|YP_001099110.1| DNA topoisomerase IV subunit B [Herminiimonas arsenicoxydans] Length = 661 Score = 1200 bits (3105), Expect = 0.0, Method: Compositional matrix adjust. Identities = 576/662 (87%), Positives = 621/662 (93%), Gaps = 1/662 (0%) Query: 1 MATKKTTPSDYSESSIRVLKGLEPVKQRPGMYTRTENPLHILQEVIDNASDEALGGYGKS 60 MATKK++ +DYSESSIRVLKGLEPVKQRPGMYTRTENPLHI+QEVIDNASDEALGGY K Sbjct: 1 MATKKSS-ADYSESSIRVLKGLEPVKQRPGMYTRTENPLHIIQEVIDNASDEALGGYCKH 59 Query: 61 IIVTINADGSVSVEDDGRGIPVGLHPEEKVPTVEIVFTRLHAGGKFDKGSGGAYAFSGGL 120 I+VT+N DGSVSVEDDGRGIPVGLHPEEK+PTVEIVFTRLHAGGKFDK SGGAYAFSGGL Sbjct: 60 IVVTLNTDGSVSVEDDGRGIPVGLHPEEKIPTVEIVFTRLHAGGKFDKSSGGAYAFSGGL 119 Query: 121 HGVGVSVTNALATRLEITVWREGGVHTLAFSGGDVIEKLKSRPATREDKKSGTRVTVWPD 180 HGVGVSVTNAL+TRLEITVWRE GVHTL FSGGDVIE LKSR R++K+SGTRVT WPD Sbjct: 120 HGVGVSVTNALSTRLEITVWRESGVHTLTFSGGDVIEPLKSRAIARDEKRSGTRVTAWPD 179 Query: 181 AKYFDSATIPLGELQRLLRSKAVLLPGVKVTLINAKNGDTQSWQYDQGLRGYLMEQLAQS 240 KYFDS+ IP ELQRLLRSKAVLLPGVKVTL++AK+G+TQ+WQYDQGLRGYL E LAQ+ Sbjct: 180 PKYFDSSVIPQAELQRLLRSKAVLLPGVKVTLVHAKSGETQTWQYDQGLRGYLTESLAQA 239 Query: 241 GSGETLIPLFEGEQFAGKDTDGFAEGEGASWVVAWTEEGAVVRESYVNLIPTSNGGTHES 300 + ET+IPLFEGEQ+A DT+GFAEGEGA+WVVAWTE+GAVVRESYVNLIPTSNGGTHES Sbjct: 240 SNAETVIPLFEGEQYANADTEGFAEGEGAAWVVAWTEDGAVVRESYVNLIPTSNGGTHES 299 Query: 301 GLREGLFSAVKSFAEMHSLLPKGVKLLAEDVFARVSFVLSAKVLDPQFQGQIKERLNSRD 360 GLREGLF AVKSF EMHSLLPKGVKLL EDVFARVSFVLSAKVLDPQFQGQIKERLNSRD Sbjct: 300 GLREGLFGAVKSFVEMHSLLPKGVKLLPEDVFARVSFVLSAKVLDPQFQGQIKERLNSRD 359 Query: 361 AVRLVAGFSRPALELWLNQHVEYGKKLAELVIKQAQSRLRSAQKVEKKKSSGVAVLPGKL 420 AVRLVA ++RPALELWLNQHV+YGKKLAELVIKQAQSRLRSAQKVEKKKSSGVAVLPGKL Sbjct: 360 AVRLVATYTRPALELWLNQHVDYGKKLAELVIKQAQSRLRSAQKVEKKKSSGVAVLPGKL 419 Query: 421 TDCESSDVTRNELFLVEGDSAGGSAKMGRDKEFQAILPLRGKVLNAWETERDRLYANNEI 480 TDCESSD +RNE+FLVEGDSAGGSAKMGRDKEFQAILPLRGKVLN+WETERDRL+ANNEI Sbjct: 420 TDCESSDTSRNEIFLVEGDSAGGSAKMGRDKEFQAILPLRGKVLNSWETERDRLFANNEI 479 Query: 481 HDIAVAIGVDPHGINDTPDLSGLRYGKICILSDADVDGSHIQVLLLTLFFRHFPKLIENG 540 HDIAVAIG+DPHG NDT D S LRYG+ICILSDADVDGSHIQVLLLTLFF+HFP+LIE G Sbjct: 480 HDIAVAIGIDPHGKNDTVDFSSLRYGRICILSDADVDGSHIQVLLLTLFFKHFPQLIERG 539 Query: 541 NICVARPPLYRVDAPARGKKPVQKLYALDDGELDAIEDKLRKDGLKEGAWSISRFKGLGE 600 +IC+ARPPLYRVDAPARGKKP+QK+YALDDGEL AIEDKLRKDG+KE AW+ISRFKGLGE Sbjct: 540 HICIARPPLYRVDAPARGKKPIQKIYALDDGELAAIEDKLRKDGVKESAWAISRFKGLGE 599 Query: 601 MNAEQLWETTMNPDTRRLLPISFGEPGVQTSEERFNMLMGKGEAASRRAWIEEHGNEAEA 660 MNAEQLWETTMNPDTRRLLP++ GE + SE RFNMLMGKGEAA+RR WIEEHGNEAEA Sbjct: 600 MNAEQLWETTMNPDTRRLLPVALGEFDLDFSEARFNMLMGKGEAAARRTWIEEHGNEAEA 659 Query: 661 DI 662 DI Sbjct: 660 DI 661 >gi|134093298|ref|YP_001098373.1| DNA gyrase subunit B [Herminiimonas arsenicoxydans] Length = 835 Score = 311 bits (798), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 207/571 (36%), Positives = 320/571 (56%), Gaps = 37/571 (6%) Query: 6 TTPSDYSESSIRVLKGLEPVKQRPGMYTRTENP----LHILQEVIDNASDEALGGYGKSI 61 T + Y SSI++L+GLE V++RPGMY + H + EV+DN+ DE+L G+ I Sbjct: 15 TLSAAYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHCVFEVLDNSIDESLAGHCTEI 74 Query: 62 IVTINADGSVSVEDDGRGIPVGLH----PEEKVPTVEIVFTRLHAGGKFDKGSGGAYAFS 117 VTI++D S+S+ D+GRGIP G+ E K EIV T LHAGGKFD+ S Y S Sbjct: 75 HVTIHSDNSISITDNGRGIPTGIKWDDKHEPKRSAAEIVMTELHAGGKFDQNS---YKVS 131 Query: 118 GGLHGVGVSVTNALATRLEITVWREGGVHTLAFSGG-------DVIEKLKSRP--ATRED 168 GGLHGVGVS N L+ L++T+ R+G H + F G + I+ P E Sbjct: 132 GGLHGVGVSCVNGLSRLLKLTIRRDGKTHYMEFVRGVPQNREIETIDGHIVSPIKVIGET 191 Query: 169 KKSGTRVTVWPDAKYFDSATIPLGELQRLLRSKAVLLPGVKVTLINAKNGDTQSWQYDQG 228 K GT V W D + F++ L + +R + L GV++ L + + G + + ++ G Sbjct: 192 DKRGTEVHFWADEEIFNNVEFHYDILAKRIRELSFLNNGVRIRLSDQRTGKEELFAFEGG 251 Query: 229 LRGYLMEQLAQSGSGETLIP-LFE--GEQFAGKDTDGFAEGEGASWVVAWTEEGAVVRES 285 RG++ E + ++ S L P +F+ GE+ + + T+ A W A+ E+ V+ Sbjct: 252 TRGFV-EYINKAKS--VLHPTIFQATGERLSDQGTN-IAVDVSMQWNDAYNEQ--VI--C 303 Query: 286 YVNLIPTSNGGTHESGLREGLFSAVKSFAEMHSLLPKG-VKLLAEDVFARVSFVLSAKVL 344 + N IP +GGTH +GLR + + + + H K V++ +D+ ++ VLS KV Sbjct: 304 FTNNIPQRDGGTHLTGLRAAMTRVINKYIDEHEFAKKAKVEVTGDDMREGLTCVLSVKVP 363 Query: 345 DPQFQGQIKERLNSRDAVRLVAGFSRPALELWLNQHVEYGKKLAELVIKQAQSRLRSAQK 404 +P+F Q K++L S + V L +L + K + +++ A++R + + Sbjct: 364 EPKFSSQTKDKLVSSEVRGPVEEIVAKTLADYLQEKPNDAKIICGKIVEAARAREAARKA 423 Query: 405 VEKKKSSGVAV---LPGKLTDCESSDVTRNELFLVEGDSAGGSAKMGRDKEFQAILPLRG 461 + + GV L KL DC+ D EL++VEGDSAGGSAK GRD++FQAILPLRG Sbjct: 424 RDLTRRKGVMDGLGLSSKLADCQEKDPALCELYVVEGDSAGGSAKQGRDRKFQAILPLRG 483 Query: 462 KVLNAWETERDRLYANNEIHDIAVAIGVDPHGINDTPDLSGLRYGKICILSDADVDGSHI 521 KVLN + +++ A+ +I + +G D ++ LRY +I I++DADVDG+HI Sbjct: 484 KVLNVEKARFEKMLASEQITTLIATLGTSIGA--DEFNIEKLRYHRIIIMTDADVDGAHI 541 Query: 522 QVLLLTLFFRHFPKLIENGNICVARPPLYRV 552 + LLLTL +R P+L+E G++ +A+PPLY+V Sbjct: 542 RTLLLTLLYRQMPQLVERGHVYIAQPPLYKV 572 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.315 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,361,078 Number of extensions: 60865 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 128 Number of HSP's successfully gapped: 2 Length of query: 662 Length of database: 1,000,347 Length adjustment: 88 Effective length of query: 574 Effective length of database: 710,387 Effective search space: 407762138 Effective search space used: 407762138 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)