BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1542.p (456 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094220|ref|YP_001099295.1| major facilitator superfamily m... 102 5e-23 gi|134096340|ref|YP_001101415.1| putative multidrug resistance-l... 82 4e-17 gi|134095839|ref|YP_001100914.1| bicyclomycin resistance protein... 57 3e-09 >gi|134094220|ref|YP_001099295.1| major facilitator superfamily multidrug transporter; membrane protein [Herminiimonas arsenicoxydans] Length = 517 Score = 102 bits (253), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 109/419 (26%), Positives = 188/419 (44%), Gaps = 29/419 (6%) Query: 24 LAIATVVLGLSI--AVLDGSIANLALPLMAADLKASDADAIWVINSYQIAMAVCLLPLAS 81 L + TV L L++ VLD SIAN+++P ++ DL S WVI S+ +A A+ + Sbjct: 17 LVVGTVALSLAVFMNVLDSSIANVSIPAISGDLGVSPQQGTWVITSFAVANAISVPLTGW 76 Query: 82 LGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALIRFIY 141 L + G R+++ + LF LAS C + + L ARVLQG A ++ ++ AL+ Y Sbjct: 77 LTQRFGQVRLFISSILLFVLASCLCGLAPSIEILIAARVLQGAVAGPMIPLSQALLLSSY 136 Query: 142 PSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGL-AALAVG----H 196 P G A+ + + +GP + G I TW W+F INIP+G+ AA A Sbjct: 137 PPAKSGMALAFWGMTTLVAPIMGPLLGGWISDNYTWPWIFYINIPIGIFAAWATWSIYRE 196 Query: 197 RALPTNPLSARPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWVVGGCTVL----TAISLV 252 R T L P D+ +L + ++G L +D L W G ++ A++ + Sbjct: 197 RESKTYKL---PIDKVGLVLLVLWVGSLQLMLDKGKELD--WFHSGEIIILASVAAVAFI 251 Query: 253 LLLRLQ-SSSAPMLPLDLLRIPLFA---LSIGTSFCSFAAQMAVFVMLPFMLQNQQGMSV 308 + + P++ L L + F+ ++I ++ F + V+LP LQ Q G + Sbjct: 252 YFIIWEIGEEHPVVDLSLFQGRNFSGGVIAISVAYGLFFGSL---VILPLWLQTQIGYTA 308 Query: 309 ARAGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLVQPGTPDGVM 368 AG ++ I + + GKL +I A + + L + T D M Sbjct: 309 TEAGKVMAPVGIFAIILSPIVGKLLPKIDARWVATTAFLIFS---LVFFMRAEFTQDVDM 365 Query: 369 VAMF---ALCGVGFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVGASVVAML 424 + + + G +F P ++S ++ A+G+ R+ +G SV++ + Sbjct: 366 MTLMIPTVIQGAAMAMFFIPLTSIILSGQSPDKIPAAAGLSNFVRIMFGGIGTSVMSTM 424 >gi|134096340|ref|YP_001101415.1| putative multidrug resistance-like efflux transmembrane protein [Herminiimonas arsenicoxydans] Length = 472 Score = 82.4 bits (202), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 93/340 (27%), Positives = 155/340 (45%), Gaps = 15/340 (4%) Query: 20 RQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSYQIAMAVCLLPL 79 R + L + T ++G A L +I N+A+P ++ A WV S+ +AM + +L Sbjct: 13 RYKWLVLLTCMIGNIAATLSATIVNVAIPDLSHYFTLGQERAQWVSTSFMLAMTLSMLTT 72 Query: 80 ASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALIRF 139 L + G RR Y G V L L + S + L RVL+GL A + + +I Sbjct: 73 PWLLQRFGLRRTYTGAVSLLLLGGVVGGFSVNYPMLLSMRVLEGLAAGVMQPIPAIVILR 132 Query: 140 IYPSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGLAALAVGHRAL 199 + + G+A+G A+GP+V G ++ L W +F + +P L + R L Sbjct: 133 AFAAEEQGKAMGIFGFGVVLTPAIGPSVGGFLVELFGWRSIFFVVVPPCLLVFYMIRRFL 192 Query: 200 PTNPL---SARPFDRQSALLC-MCFIGLL--LYTIDAVGHLQGAWVVGGCTVLTAISLVL 253 P A+P D + LL + I LL L + A ++ G TA + Sbjct: 193 PLYSQILEHAKPLDWKGLLLIGISIISLLNGLVELRADLQQALLLLLLGVGSFTAF---I 249 Query: 254 LLRLQSSSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVF---VMLPFMLQNQQGMSVAR 310 + +L++ P++ + L FA+ + +F M +F +LP LQ + ++ Sbjct: 250 VYQLRTRHEPLVNMALFGYRQFAMG---ALVAFIYGMGLFGSTYLLPVYLQVALHYAPSQ 306 Query: 311 AGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMC 350 AGL+L I +ALT +AG+L+DR +L IGL ++ Sbjct: 307 AGLVLLPAGIVLALTIPLAGRLADRYPPNVLVVIGLGLLT 346 >gi|134095839|ref|YP_001100914.1| bicyclomycin resistance protein [Herminiimonas arsenicoxydans] Length = 422 Score = 56.6 bits (135), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 43/169 (25%), Positives = 77/169 (45%), Gaps = 2/169 (1%) Query: 46 ALPLMAADLKASDADAIWVINSYQIAMAVCLLPLASLGEVIGYRRVYLGGVFLFTLASLA 105 A P + L AS + + +Y + AV +L +L + G R + L + +F +A+L Sbjct: 43 AFPNIQTSLGASALEVQQTLTAYLFSFAVMILWHGALSDAFGRRNIILVSLAVFAVATLG 102 Query: 106 CAASGDMLQLSLARVLQGLGAAGILSVNTALIRFIYPSRLLGRAIGYNALVAGTGNALGP 165 CAA+ + L R+LQG+ A + + A+IR +Y + + ++ A+ P Sbjct: 103 CAAAHSIEYLWAFRMLQGISAGAGVVIGRAIIRDLYAGAEAEKLLSLVTMIFSIAPAIAP 162 Query: 166 TVAGLILHLATWHWLFLINIPLGLAALAVGHRALPTN--PLSARPFDRQ 212 + G I+ W +FL + L ++ LP + P PF+ Q Sbjct: 163 MLGGWIVKRMDWRAIFLALFAYAVLLLLYCYKRLPESLPPEKRHPFNPQ 211 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.327 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 766,263 Number of extensions: 29810 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 3 Length of query: 456 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 371 Effective length of database: 720,272 Effective search space: 267220912 Effective search space used: 267220912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)