BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1479.p (546 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 140 1e-34 gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Hermi... 86 5e-18 gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 84 3e-17 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 151/555 (27%), Positives = 250/555 (45%), Gaps = 47/555 (8%) Query: 14 YPLLIKQLLHSALATAPEQEIIYGQRRLSYAQFYQRVQRLANALKEMGVQAGNTVAVMDW 73 Y L++ L S A + + L+YA+ Q+L L+ G+Q G VA+M Sbjct: 22 YDSLVELLEESFRKYADRHAYVCMDKFLTYAEVDTLSQKLGAWLQGTGLQKGARVALMMP 81 Query: 74 DSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLMNSDFMPVLKQIRGEL 133 + +Y AV G V+ VN P ++ + L + A+ +++ +F L++I + Sbjct: 82 NVLQYPVALAAVLRAGYVVVNVNPLYKPRELQHQLVDSGAEAIIILENFATTLEKII--V 139 Query: 134 STLTRCVLLTDDGSPCP--RG--------------PGF----AGEYEQLLAQA-EARYDF 172 ST + V++ G +G P F A ++ +LAQA Sbjct: 140 STQIKHVVVATMGDMLGGLKGLLVNFVVRHVKKMVPAFSLPQAVSFKDVLAQAAHMHLQR 199 Query: 173 PDFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLATTAGLALPAQQGRLHRDDVYMPL 232 P A YT GTTG KG +HR +V + L A + ++ + ++ Sbjct: 200 PLLGPQDIAFLQYTGGTTGTAKGAMLTHRNIVANILQNEAWIWPVLRKEPPDKTLNFVCA 259 Query: 233 TPMFHVHAW-GLPYVATMAGVKQVY-PG-RYVPDTICKLIREEKVTFSHCVPTILQMILS 289 P++H+ A + T GV + P R +P + +L++ K+ V T+ +L+ Sbjct: 260 LPLYHIFALTACCMLGTRLGVMNILIPNPRDIPSLVKELMKY-KINLLPAVNTLYNGLLN 318 Query: 290 SEKAREVDLSGWKMIIGGSAMTSSLA--RAARERGIDVFTGYGMSETCPILTLAQVPTAE 347 + +VD S K+ GG + R G + GYG+SET P+ T V T E Sbjct: 319 NPDFAKVDFSELKLCNGGGMAVQQVVNDRWRVVTGCSIIEGYGLSETSPVATSNPVETTE 378 Query: 348 LGGEREASLRVKTGRPVPLVALRTVDGDMNDCAHDGQSTGEVVVRAPWLTQGYLHQ-AEA 406 G G PVP + +D + N+ A +GE+ +R P + GY + AE Sbjct: 379 FSG--------TIGLPVPSTEVMILDSNDNEVALG--LSGEIAIRGPQVMAGYWNNPAET 428 Query: 407 SEELWRGGWLHTQDIGHIDGEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETA 466 ++ + G+ T DIG +D GY ++ DR KD+I G V +IE+++A HP V E A Sbjct: 429 AQAMTADGFFKTGDIGVMDARGYTRIVDRKKDMILVSGFNVYPSEIENVVAGHPGVLECA 488 Query: 467 VIGVQDGKWGERPVALVVLKKDAVGVSEEDIRQHVRASAEAGVISRYGVPDRVRFVDELA 526 IGV D GE V L V+++D ++ E + + + Y P + F EL Sbjct: 489 CIGVPDEHTGEA-VKLFVVRRDP-ALTAEALMAFCKEQ-----FTGYKKPKMIEFRTELP 541 Query: 527 RTSVGKINKRVMREQ 541 +++VGKI +R +R++ Sbjct: 542 KSNVGKILRRELRDE 556 >gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 634 Score = 85.9 bits (211), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 140/557 (25%), Positives = 240/557 (43%), Gaps = 72/557 (12%) Query: 25 ALATAPEQEIIYGQRRLSYAQFYQRVQRLANALKEMGVQAGNTVAVMDWDSNRYLECFFA 84 A++T + E +Y ++AQ + V R A ++ +GV G+ V + A Sbjct: 78 AISTETDSEKVY-----TFAQLHAEVNRCAAMMQSLGVSQGDRVLIYMPMVAEAAFAMLA 132 Query: 85 VPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLMNSD-------FMPVLKQIRGELSTLT 137 +G + V + +A ++ A VL++++D MP K + E L Sbjct: 133 CARIGAIHSVVFGGFASNSLATRIDDATP-VLILSADAGSRGCHVMP-YKSLLDEAIVLA 190 Query: 138 RC----VLLTDDG-SPCPRGPGFAGEYEQLLAQ-AEARYDFPDFDENARATTFYTTGTTG 191 VL+ + G +P G +Y L + +A + NA + YT+GTTG Sbjct: 191 EHKPTHVLMVNRGLAPMQTVAGRDIDYATLREKHMDAAVPVVWLESNAPSYILYTSGTTG 250 Query: 192 LPKGVYFSHRQLVLHTLATTAGLAL----PAQQGRLHRDDVYMPLTPMFHVHAWGLPYVA 247 PKGV R + + +A T + L A + D+ + + ++ P +A Sbjct: 251 KPKGVV---RDVGGYAVALTTSMDLIFCGKAGETFFCTSDIGWVVGHSYIIYG---PLLA 304 Query: 248 TMAGVKQVYPGRYV-PDTIC--KLIREEKVTFSHCVPTILQMILSS--EKAREVDLSGWK 302 MA V +Y G + PD ++ + KVT PT ++++ E ++ DLS K Sbjct: 305 GMATV--LYEGLPIRPDASIWWSIVEKYKVTRMSSAPTAIRVLKKQPLELLKKHDLSSLK 362 Query: 303 -MIIGGSAMTSSLAR-AARERGIDVFTGYGMSET-CPILTLAQVPTAELGGEREASLRVK 359 + + G + + + + + V Y +ET PI+++A+ G + R Sbjct: 363 ALYLAGEPLDQTTSEWISGVLNVPVIDNYWQTETGWPIISIAK-------GVGDKPTR-- 413 Query: 360 TGRP-VPLVALRT-----VDGDMNDCAHDGQSTGEVVVRAPWLTQGYLHQAEASEE---- 409 +G P +PL +T + G+ +CA G V++ P L G L ++ Sbjct: 414 SGSPGLPLYGYKTKIINEISGE--ECA--ANEKGVVIIEGP-LPPGCLQTLYGDDQRFVS 468 Query: 410 -LWRG---GWLHTQDIGHIDGEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAET 465 WR T D G D +GY + R DVI G + + +IE+ I+ H V+E Sbjct: 469 TYWRNFKREAYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHGNVSEV 528 Query: 466 AVIGVQDGKWGERPVALVVLKKDAVGVSEEDIRQHVRASAEAGVISRYGV---PDRVRFV 522 AV+GV++ G+ VA V+LK A+ S E+ ++ + A V R G P V FV Sbjct: 529 AVVGVENPIKGQCAVAFVILKNAALAASPEEAKK-LEAEIMKVVDKRLGSIGRPSHVYFV 587 Query: 523 DELARTSVGKINKRVMR 539 L +T GK+ +R ++ Sbjct: 588 SALPKTRSGKLLRRSIQ 604 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 83.6 bits (205), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 133/555 (23%), Positives = 226/555 (40%), Gaps = 90/555 (16%) Query: 40 RLSYAQFYQRVQRLANALKEMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRL 99 R++Y + ++RV + AN LK + + G+ V + S + A +G V Sbjct: 113 RITYKELHERVCKFANGLKSLSINKGDRVIIYMPMSIDGIAAMQACARIGATHSVVFGGF 172 Query: 100 SPEQIAYTLNHAQADVLLMNSDFMPVLKQIRGELSTLTRCVL---LTDDGSPCPRG---- 152 S + + + A A V ++ +D+ Q+RG + + ++ L G R Sbjct: 173 SAKSLHERIIDAGA-VAVLTADY-----QVRGGKNLPLKAIVDEALAMGGCDTIRNVVVY 226 Query: 153 --PGFAGEY--------EQLLAQAEARYDFPDFDENARATTFYTTGTTGLPKGVYFSHRQ 202 G A ++ +L+ Q + YT+G+TG PKGV S Sbjct: 227 KRTGGAVDFVERRDIWLHELVEQRTSECAPEPVGAEHPLFILYTSGSTGKPKGVQHSSAG 286 Query: 203 LVLHTLATTAGLALPAQQGRLHRDDVYMPLTPMFHV--HAWGLPYVATMAGVKQV----- 255 +L + T + + +DVY + V H + + Y G Q+ Sbjct: 287 YLLWAMLTMKWVF------DIKPNDVYWCTADIGWVTGHTY-VTYGPLAVGATQIVFEGV 339 Query: 256 --YP--GRYVPDTICKLIREEKVTFSHCVPTILQMILSSEKA------REVDLSGWKMII 305 YP GR+ +++++ KV+ + PT ++ ++ + +A + DLS +++ Sbjct: 340 PMYPNAGRF-----WEMVQKHKVSIFYTAPTAIRSLIKAAEADPSTHPSKYDLSSLRLL- 393 Query: 306 GGSAMTSSLARAARERGIDVFTGYGMSETCPIL-TLAQVPTAELGGEREASLRVKTGR-- 362 S+ + E CPI+ T Q E GG + L T Sbjct: 394 ------GSVGEPINPEAWMWYYKNIGGERCPIVDTFWQT---ETGGHVISPLPGATPMVP 444 Query: 363 -----PVPLVALRTVDGDMNDCAHD--GQSTGEVVVRAPW--LTQGYLHQAEASEELW-- 411 P+P + VD D HD G +V++ PW + + + E E+ + Sbjct: 445 GSCTLPLPGIIAAVVD----DGGHDLPNGKGGLLVIKRPWPAMIRNIWNDPERFEKSYFP 500 Query: 412 --RGGWLHTQDIGHIDGE--GYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAV 467 GG + G I E GY +T RI DV+ G + + +IE + +P VAE AV Sbjct: 501 HELGGRTYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMGTAEIESALVANPMVAEAAV 560 Query: 468 IGVQDGKWGERPVALVVLKK-DAVGVSEEDIRQHVR--ASAEAGVISRYGVPDRVRFVDE 524 +G D GE A VVLK+ G + I + +R + E G I++ P +RF D Sbjct: 561 VGKPDETTGESICAFVVLKRVRPEGDEAKQIAKELRDWVAKEIGPIAK---PKEIRFGDN 617 Query: 525 LARTSVGKINKRVMR 539 L +T GKI +R++R Sbjct: 618 LPKTRSGKIMRRLLR 632 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,019,293 Number of extensions: 41219 Number of successful extensions: 106 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 3 Length of query: 546 Length of database: 1,000,347 Length adjustment: 86 Effective length of query: 460 Effective length of database: 716,977 Effective search space: 329809420 Effective search space used: 329809420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)