BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1382.p (660 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 1143 0.0 gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Hermi... 421 e-119 gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 90 4e-19 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 1143 bits (2957), Expect = 0.0, Method: Compositional matrix adjust. Identities = 532/660 (80%), Positives = 599/660 (90%) Query: 1 VADIETFKQENRVFAPPAELAKHAAISGMDAYHALNAEFANDYEGTWARLAKDNLKWHKP 60 ++ IE+FKQE RVF P + +AAISGM+AY AL AE A+DYEG WARLA+ NL W KP Sbjct: 1 MSSIESFKQEGRVFDSPVDFVNNAAISGMEAYRALCAEAADDYEGFWARLAQQNLHWTKP 60 Query: 61 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAIIFESDDGQVTRVSYRELH 120 FT+ LDESNAPFYKWF DG++NVS+NCLD +L NGNADK AIIFE+DDG VTR++Y+ELH Sbjct: 61 FTKKLDESNAPFYKWFEDGELNVSWNCLDRHLHNGNADKVAIIFEADDGNVTRITYKELH 120 Query: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 ++VC+FANGLKSL I KGDRV+IYMPMS++G+AAMQACARIGATHSVVFGGFSAKSLHER Sbjct: 121 ERVCKFANGLKSLSINKGDRVIIYMPMSIDGIAAMQACARIGATHSVVFGGFSAKSLHER 180 Query: 181 VIDAGAVAVVTADYQVRGGKQLPLKSIVDEALAMGGCDTLKQVIVYKRTGAELNWVEGRD 240 +IDAGAVAV+TADYQVRGGK LPLK+IVDEALAMGGCDT++ V+VYKRTG +++VE RD Sbjct: 181 IIDAGAVAVLTADYQVRGGKNLPLKAIVDEALAMGGCDTIRNVVVYKRTGGAVDFVERRD 240 Query: 241 LWLSDVMANQAATCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD 300 +WL +++ + + C PE V AEHPLFILYTSGSTGKPKGVQH+SAGYLLWA+LTMKW FD Sbjct: 241 IWLHELVEQRTSECAPEPVGAEHPLFILYTSGSTGKPKGVQHSSAGYLLWAMLTMKWVFD 300 Query: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF 360 IKP+DVYWCTADIGW+TGHTY+ YGPLAVGATQIVFEGVP YPNAGRFW+MV +HK +IF Sbjct: 301 IKPNDVYWCTADIGWVTGHTYVTYGPLAVGATQIVFEGVPMYPNAGRFWEMVQKHKVSIF 360 Query: 361 YTAPTAIRSLIKAADADEKVHPKQYDLSTLRILGSVGEPINPEAWMWYYKNVGRENCPIV 420 YTAPTAIRSLIKAA+AD HP +YDLS+LR+LGSVGEPINPEAWMWYYKN+G E CPIV Sbjct: 361 YTAPTAIRSLIKAAEADPSTHPSKYDLSSLRLLGSVGEPINPEAWMWYYKNIGGERCPIV 420 Query: 421 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGHDLPNGNGGILVVKRPW 480 DTFWQTETGGH+ISPLPGATP VPGSCTLPLPGI AA+VD+ GHDLPNG GG+LV+KRPW Sbjct: 421 DTFWQTETGGHVISPLPGATPMVPGSCTLPLPGIIAAVVDDGGHDLPNGKGGLLVIKRPW 480 Query: 481 PSMIRTIWNDPERFKKSYFPEELGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 540 P+MIR IWNDPERF+KSYFP ELGG YLAGDGA+RN+ETGYFTITGRIDDVLN+SGHRM Sbjct: 481 PAMIRNIWNDPERFEKSYFPHELGGRTYLAGDGAIRNEETGYFTITGRIDDVLNISGHRM 540 Query: 541 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVA 600 GT EIESALVAN +VAEAAVVGKPDETTGESICAFVVLKR RP G+EAKQIAKELRDWVA Sbjct: 541 GTAEIESALVANPMVAEAAVVGKPDETTGESICAFVVLKRVRPEGDEAKQIAKELRDWVA 600 Query: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 660 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAK E ITQD+STLENPAIL+QLK+AQ Sbjct: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKDEDITQDISTLENPAILEQLKQAQ 660 >gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 634 Score = 421 bits (1082), Expect = e-119, Method: Compositional matrix adjust. Identities = 245/637 (38%), Positives = 369/637 (57%), Gaps = 21/637 (3%) Query: 31 AYHALNAEFANDYEGTWARLAKDNLKWHKPFTQTLDESNAPFYKWFADGQVNVSYNCLDV 90 AY A +D WA A+ ++W+KPFT+TLD + PF KWF G+ ++ YN +D Sbjct: 7 AYDAFYRRSIDDPHTFWAEQAQ-RIEWNKPFTRTLDYDHPPFTKWFVGGETSLCYNAVDR 65 Query: 91 NLENGNADKTAIIFESDDGQVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSV 149 +L AD+ A+I S + +V ++ +LH +V + A ++SLG+ +GDRV+IYMPM Sbjct: 66 HLAT-RADQPALIAISTETDSEKVYTFAQLHAEVNRCAAMMQSLGVSQGDRVLIYMPMVA 124 Query: 150 EGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVVTADYQVRGGKQLPLKSIVD 209 E AM ACARIGA HSVVFGGF++ SL R+ DA V +++AD RG +P KS++D Sbjct: 125 EAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPVLILSADAGSRGCHVMPYKSLLD 184 Query: 210 EALAMGGCDTLKQVIVYKRTGAELNWVEGRDLWLSDVMANQAATCEP-EWVDAEHPLFIL 268 EA+ + V++ R A + V GRD+ + + P W+++ P +IL Sbjct: 185 EAIVLAEHKP-THVLMVNRGLAPMQTVAGRDIDYATLREKHMDAAVPVVWLESNAPSYIL 243 Query: 269 YTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLA 328 YTSG+TGKPKGV GY + +M F K + ++CT+DIGW+ GH+YI YGPL Sbjct: 244 YTSGTTGKPKGVVRDVGGYAVALTTSMDLIFCGKAGETFFCTSDIGWVVGHSYIIYGPLL 303 Query: 329 VGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLS 388 G +++EG+P P+A +W +V ++K T +APTAIR L K K H DLS Sbjct: 304 AGMATVLYEGLPIRPDASIWWSIVEKYKVTRMSSAPTAIRVLKKQPLELLKKH----DLS 359 Query: 389 TLRILGSVGEPINPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPLPGA--TPQVPGS 446 +L+ L GEP++ W G N P++D +WQTETG +IS G P GS Sbjct: 360 SLKALYLAGEPLDQTTSEWI---SGVLNVPVIDNYWQTETGWPIISIAKGVGDKPTRSGS 416 Query: 447 CTLPLPGITAAIVDE-TGHDLPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELG 504 LPL G I++E +G + G+++++ P P ++T++ D +RF +Y+ Sbjct: 417 PGLPLYGYKTKIINEISGEECAANEKGVVIIEGPLPPGCLQTLYGDDQRFVSTYW-RNFK 475 Query: 505 GNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKP 564 Y D +R+ + GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG Sbjct: 476 REAYSTFDWGIRDAD-GYYFILGRTDDVINVAGHRLGTREIEESISSHGNVSEVAVVGVE 534 Query: 565 DETTGESICAFVVLKRP--RPTGEEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTR 622 + G+ AFV+LK + EEAK++ E+ V K +G I +P + F LPKTR Sbjct: 535 NPIKGQCAVAFVILKNAALAASPEEAKKLEAEIMKVVDKRLGSIGRPSHVYFVSALPKTR 594 Query: 623 SGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEA 659 SGK++RR ++ + +G D+S +E+P+ L Q++ A Sbjct: 595 SGKLLRRSIQAICEGRD-PGDLSVIEDPSALQQIRTA 630 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 89.7 bits (221), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 66/216 (30%), Positives = 101/216 (46%), Gaps = 15/216 (6%) Query: 417 CPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGHDLPNGNGGILVV 476 C I++ + +ET S P T + G+ LP+P I+D +++ G G + + Sbjct: 354 CSIIEGYGLSETSPVATSN-PVETTEFSGTIGLPVPSTEVMILDSNDNEVALGLSGEIAI 412 Query: 477 KRPWPSMIRTIWNDPERFKKSYFPEELGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVS 536 + P ++ WN+P ++ + + GD V + GY I R D++ VS Sbjct: 413 R--GPQVMAGYWNNPAETAQAMTAD----GFFKTGDIGVMDAR-GYTRIVDRKKDMILVS 465 Query: 537 GHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELR 596 G + EIE+ + + V E A +G PDE TGE++ FVV + P T E KE Sbjct: 466 GFNVYPSEIENVVAGHPGVLECACIGVPDEHTGEAVKLFVVRRDPALTAEALMAFCKE-- 523 Query: 597 DWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 632 + KPK I F LPK+ GKI+RR LR Sbjct: 524 -----QFTGYKKPKMIEFRTELPKSNVGKILRRELR 554 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,386,715 Number of extensions: 62440 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 133 Number of HSP's successfully gapped: 3 Length of query: 660 Length of database: 1,000,347 Length adjustment: 88 Effective length of query: 572 Effective length of database: 710,387 Effective search space: 406341364 Effective search space used: 406341364 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)