BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1348.p (257 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093876|ref|YP_001098951.1| tRNA (guanine-N(1)-)-methyltran... 401 e-113 >gi|134093876|ref|YP_001098951.1| tRNA (guanine-N(1)-)-methyltransferase [Herminiimonas arsenicoxydans] Length = 248 Score = 401 bits (1030), Expect = e-113, Method: Compositional matrix adjust. Identities = 187/248 (75%), Positives = 214/248 (86%) Query: 1 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 60 MQFDVVTLFPEMF A+TQSGITRRA EQ++ L LWNPR+FT+D HRTVDDRPYGGGPGM Sbjct: 1 MQFDVVTLFPEMFTAITQSGITRRAFEQKKCELALWNPRDFTSDRHRTVDDRPYGGGPGM 60 Query: 61 VMLAKPLQAAIEAAQQRQQTLGLAKPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 120 VM+ KPL+AA+ A++RQ L PRVIYLSPQG LTHERV++L+ + G VLLCGRYE Sbjct: 61 VMMVKPLEAAVLEAKKRQIATDLPTPRVIYLSPQGKALTHERVMQLTAEPGLVLLCGRYE 120 Query: 121 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 180 A+DQR LD CVDEEIS+GDFVLSGGELPAMALMDAVIR LPG L+D ASAVEDSFVNGLL Sbjct: 121 AVDQRFLDVCVDEEISLGDFVLSGGELPAMALMDAVIRQLPGVLHDDASAVEDSFVNGLL 180 Query: 181 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQAKRPDLIIRAREQGLLSKA 240 D PHYTRPEVYEG VP++L+GGHH EI+KWRR+RALEAT KRPD+I++AR GLL++A Sbjct: 181 DCPHYTRPEVYEGVAVPAVLMGGHHVEIEKWRRERALEATAKKRPDMIVKARAAGLLTRA 240 Query: 241 DEKFLGSL 248 DEKFL SL Sbjct: 241 DEKFLSSL 248 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 491,647 Number of extensions: 19633 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 40 Number of HSP's successfully gapped: 1 Length of query: 257 Length of database: 1,000,347 Length adjustment: 80 Effective length of query: 177 Effective length of database: 736,747 Effective search space: 130404219 Effective search space used: 130404219 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)