BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1312.p (359 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096382|ref|YP_001101457.1| tartrate dehydrogenase [Hermini... 558 e-160 gi|134094446|ref|YP_001099521.1| 3-isopropylmalate dehydrogenase... 167 5e-43 gi|134096423|ref|YP_001101498.1| putative isocitrate dehydrogena... 144 5e-36 >gi|134096382|ref|YP_001101457.1| tartrate dehydrogenase [Herminiimonas arsenicoxydans] Length = 356 Score = 558 bits (1438), Expect = e-160, Method: Compositional matrix adjust. Identities = 260/359 (72%), Positives = 306/359 (85%), Gaps = 5/359 (1%) Query: 1 MSKTHRIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPD 60 M+ + IA+I GDGIG+EV+PEGLR ++AA+K+FGI +E+ H +WA C YYA+ G+MMPD Sbjct: 1 MNPSFNIAIIPGDGIGKEVIPEGLRVLDAAAKRFGIHLEYKHIEWASCDYYAQTGKMMPD 60 Query: 61 DWFEQLKGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLA 120 DW +QL+G +AI+FGAVGWPATVPDHVSLWGSLLKFRR+FD YVNLRPVRL GVPCPLA Sbjct: 61 DWKQQLQGCDAIYFGAVGWPATVPDHVSLWGSLLKFRREFDQYVNLRPVRLFEGVPCPLA 120 Query: 121 NRKPGDIDFYVVRENTEGEYSAIGGRMFEGTERETVIQESVFSRKGTDRILKFAFDLAQS 180 NRKPGDIDFYVVRENTEGEY+AIGG M+EGTERE V+Q+S FSR+GTDRILK+AF+LAQS Sbjct: 121 NRKPGDIDFYVVRENTEGEYTAIGGIMYEGTERELVLQQSAFSRRGTDRILKYAFELAQS 180 Query: 181 RPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVV 240 R ++ LT ATKSNGIAI+MP+WD R + M YPD+ DK HIDIL A FVL PDRFDVV Sbjct: 181 RARRRLTVATKSNGIAISMPWWDSRADEMAKNYPDITVDKQHIDILAARFVLQPDRFDVV 240 Query: 241 VASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAT 300 VASNLFGDILSDLGPAC GT+G+APSAN+NP R PSLFEPVHGSAPDI+G+NIANP+A Sbjct: 241 VASNLFGDILSDLGPACTGTMGLAPSANLNPERLFPSLFEPVHGSAPDIYGQNIANPIAM 300 Query: 301 IWSGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 IWSGA+MLDFLG R AHDA+L AI+QVL +GP TPDLGG + T ++G AIAAA+ Sbjct: 301 IWSGALMLDFLGQ-----RAAHDAILEAIKQVLAHGPRTPDLGGNAGTADIGMAIAAAV 354 >gi|134094446|ref|YP_001099521.1| 3-isopropylmalate dehydrogenase [Herminiimonas arsenicoxydans] Length = 356 Score = 167 bits (424), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 129/369 (34%), Positives = 186/369 (50%), Gaps = 30/369 (8%) Query: 6 RIAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPDDWFEQ 65 +IA++ GDGIG E+M + ++ + A +KF T YA+ G +P+ + Sbjct: 2 KIAILPGDGIGPEIMNQAVKVMNALGEKFE-----TETVPVGGAAYAEQGHPLPESTLKV 56 Query: 66 LKGFEAIFFGAVG--WPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLANRK 123 K +AI FGAVG T+ + ++L R+ L+ N RP L P + ++ K Sbjct: 57 AKEADAILFGAVGDFKYDTLERSLRPEQAILGLRKHLGLFANFRPAILYPEL-AGASSLK 115 Query: 124 P---GDIDFYVVRENTEGEYSAIGGRMFEGTE------RETVIQESVFSRKGTDRILKFA 174 P +D +VRE T Y M E + RE +S RI A Sbjct: 116 PEIVSGLDIMIVRELTGDIYFGQPRGMREAPDGPFKGAREG-FDTMRYSEPEIRRIAHVA 174 Query: 175 FDLAQSRPKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNP 234 F AQ R K+ LTS K+N + T +W + V + +Y DV+ D ++D V P Sbjct: 175 FQAAQKRSKR-LTSVDKAN-VLETFQFWRDVVTDVHKEYKDVKLDHMYVDNAAMQLVRAP 232 Query: 235 DRFDVVVASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNI 294 FDVVV N+FGDILSD G+IG+ PSA+++ + L+EP HGSAPDI GK+I Sbjct: 233 KEFDVVVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNK--GLYEPSHGSAPDIAGKDI 290 Query: 295 ANPVATIWSGAMMLDFLGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDL----GGKSNTTE 350 ANP+A I S AMML + GK QA D + A++ VL G T D+ K +TT+ Sbjct: 291 ANPLAQILSLAMMLRY---SLGKTEQA-DRIETAVKAVLKQGLRTVDIYEAGTTKVSTTQ 346 Query: 351 VGQAIAAAI 359 +G A+ A+ Sbjct: 347 MGDAVVKAL 355 >gi|134096423|ref|YP_001101498.1| putative isocitrate dehydrogenase [NAD] subunit alpha [Herminiimonas arsenicoxydans] Length = 348 Score = 144 bits (364), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 116/358 (32%), Positives = 171/358 (47%), Gaps = 30/358 (8%) Query: 7 IAVIAGDGIGQEVMPEGLRAVEAASKKFGIDIEFTHFDWAHCGYYAKHGQMMPDDWFEQL 66 + +I GDGIG E++ +R +A F + + A K G ++P + + Sbjct: 12 VTLIPGDGIGPEIVDVVVRVFDALGNPFAWETQ-----QAGVNALEKSGDLLPQVTLDSI 66 Query: 67 KGFEAIFFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVR-LMPGVPCPLANRKPG 125 G P + P ++ R F LY N+RP R ++PG + Sbjct: 67 TRTGLALKG----PLSTPIGGGFRSVNVRLRETFQLYANVRPARTIVPG-------GRYE 115 Query: 126 DIDFYVVRENTEGEYSAIGGRMFEGTERETV-IQESVFSRKGTDRILKFAFDLAQSRPKK 184 ID +VREN EG Y + G + V + + +R G+ RI KFAFD A +K Sbjct: 116 KIDLVLVRENLEGLYVGHEHYVPIGDDAHAVAMATGINTRAGSRRISKFAFDYAVRNNRK 175 Query: 185 HLTSATKSNGIAITMPYWDERVEAMGAKYPD-VRRDKYHIDILTAHFVLNPDRFDVVVAS 243 +T K+N + + E + +G Y + V D +D VLNP +FDV+V++ Sbjct: 176 KVTIVHKANVLKALTGLFLETAKQVGLNYANQVEFDDRIVDACAMQLVLNPWQFDVIVST 235 Query: 244 NLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVATIWS 303 NLFGDILSD G +G+AP ANI E ++FE VHGSAPDI GK +ANP A + + Sbjct: 236 NLFGDILSDQIAGLVGGLGMAPGANIG---ERTAIFEAVHGSAPDIAGKGMANPTALLLA 292 Query: 304 GAMMLDF--LGNGEGKYRQAHDAMLAAIEQVLVNGPTTPDLGGKSNTTEVGQAIAAAI 359 A+ML+ L G R A D++L+ T DLGG +T++ G + I Sbjct: 293 AALMLEHKGLNTQAGLLRDAIDSVLST------ESGRTFDLGGTVSTSKYGDLLIERI 344 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.321 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 755,259 Number of extensions: 33383 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 3 Length of query: 359 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 276 Effective length of database: 726,862 Effective search space: 200613912 Effective search space used: 200613912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)