BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1297.p (492 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrog... 305 5e-84 gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde deh... 175 4e-45 gi|134095327|ref|YP_001100402.1| multifunctional: transcriptiona... 153 2e-38 >gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrogenase I, NADP-dependent [Herminiimonas arsenicoxydans] Length = 492 Score = 305 bits (780), Expect = 5e-84, Method: Compositional matrix adjust. Identities = 169/475 (35%), Positives = 271/475 (57%), Gaps = 11/475 (2%) Query: 12 IAGQWRLGSGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHERA 71 I G W G+ + PA G ++ + + E AI A AF + WA K ERA Sbjct: 23 IDGTWA-GAATNFDISNPADGSIIGSAPNMGAEETETAIRAAQSAFPA--WAAKTGKERA 79 Query: 72 AVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAAACETLE-ETLTP 130 ++ + LI + LA + + GKP+ E+R V A+ ++F+ + + + + Sbjct: 80 IIMRQWFDLIIANVDDLAILMTAEQGKPLIESRGEVIYGASFIEWFSEEAKRVSGDVMAS 139 Query: 131 MRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELA 190 D T+++ +P+G+ AAITPWN PIA +K+APA+AAG +V+KPAE TPL+AL +A Sbjct: 140 TWNDKRTLTIKQPIGICAAITPWNFPIAMITRKVAPAVAAGCTIVIKPAEQTPLSALAIA 199 Query: 191 RICIEAGVPAGMISVLPGKGS---VIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMM 247 + AG+PAG+I+V+ V+G + P+V+ VSFTG T G+ + A + Sbjct: 200 ELAHRAGLPAGVINVITADAENSIVVGKTLCASPIVRHVSFTGSTPVGRILMEQCAPTVK 259 Query: 248 PVSLELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLA 307 ++LELGG +P +VF+DAD+D AV G L + ++G++C+ +R +V ++YD F+++LA Sbjct: 260 KIALELGGHAPFIVFDDADLDAAVTGALQSKYRNAGQTCVCTNRFYVHESIYDVFVQKLA 319 Query: 308 AAAAALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGY 367 A AA ++VG+ + GPLI + + +E++V V +G + TGG + G+ Sbjct: 320 AGAARIKVGNGFEQGVLQGPLIDDQAIRKVEQHVSDAVKKGASVMTGGKK----HALGGH 375 Query: 368 YYTPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRA 427 +Y PT++ +++ I EE FGPV + F ED+ IA AN++ + LA+ + RD R Sbjct: 376 FYEPTVLSNITDDMSIMHEETFGPVAAIVKFKTEDEAIAAANNTDFGLASYFYARDISRV 435 Query: 428 WRIGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWG 482 WR+ ++ G V INT + PFGG + SGLGRE + GI +Y+E K + G Sbjct: 436 WRVAEKLEYGMVGINTGLISNEVAPFGGVKQSGLGREGSKYGIDEYLEIKYLCMG 490 >gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde dehydrogenase, oxidoreductase protein [Herminiimonas arsenicoxydans] Length = 506 Score = 175 bits (444), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 135/463 (29%), Positives = 217/463 (46%), Gaps = 19/463 (4%) Query: 29 PATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHERAAVLYRVAQLIRERSEAL 88 PATGE+ A + AS ADV+ A+A A A + A +RA +L++ LI L Sbjct: 31 PATGEISANVVLASDADVQTAVAAAKAAAPAW--AEMAPLKRARILFKFKDLIERHHNDL 88 Query: 89 AQMQRLDNGKPISETRALVASAAATFQFFAAACETLEETLTPMRGDNL-TMSLYEPMGVV 147 A ++GK S+ + V +F + L+ + G + +L +P+GV Sbjct: 89 AAAITREHGKVFSDAKGEVTRGLEVVEFACGVPQLLKTQFSDNIGGGIDNWNLRQPLGVT 148 Query: 148 AAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELARICIEAGVPAGMISVLP 207 A ITP+N P ALA GN ++KP+E P +L +A + +AG+P G+ +VL Sbjct: 149 AGITPFNFPFMVPMWMAPVALATGNTFILKPSERDPSPSLMVAELLHQAGLPDGVFNVLQ 208 Query: 208 GKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVSLEL----GGKSPTMVFE 263 G + D + +HP +K VSF G T ++I A + L + G K+ +V Sbjct: 209 GDKRAV-DGLLQHPDIKAVSFVGSTPIAEYIYQAGAARKGSYPLRVQALGGAKNHLVVMP 267 Query: 264 DADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAAAALRVGDPADART 323 DA+++ AV ++ + S+GE C+A S ++ D + L +L+V + ++ Sbjct: 268 DANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVAALIPRVKSLKVKNGMESDA 327 Query: 324 QMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGYYYTPTIIEGVSNQQRI 383 +MGP+++A H+ IE Y+E G+ G ++ G G++ + + V+ + I Sbjct: 328 EMGPVVSAAHKAKIEGYIEAGIKSGAKLLVDGRGLTVDGHSNGFFLGGCLFDNVTPEMTI 387 Query: 384 CQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWRIGRAVQAGNVWINT 443 QEEIFGPVL + D + N + ++T D A R V+ G V IN Sbjct: 388 YQEEIFGPVLCVVRVPDFSAALDLINAHEFGNGVALFTSDGNTAREFSRRVEIGMVGINV 447 Query: 444 YKQFSISTP-----FGGWRDSGLGREK--GRLGILQYMEQKAM 479 I P FGGW+ S G G GI Y K++ Sbjct: 448 ----PIPVPMAWHSFGGWKRSMFGDTHAYGEEGIKFYTRYKSI 486 >gi|134095327|ref|YP_001100402.1| multifunctional: transcriptional repressor of proline utilization; proline dehydrogenase; pyrroline-5-carboxylate dehydrogenase [Herminiimonas arsenicoxydans] Length = 1224 Score = 153 bits (386), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 128/469 (27%), Positives = 208/469 (44%), Gaps = 49/469 (10%) Query: 33 EVVATLHAASVADVEEA----IAGADLAFRSSGWAHKKAHERAAVLYRVAQLIRERSEAL 88 +V+ T A+ DVE A IAGA+ W ERA +L A+L ++ + Sbjct: 590 DVLGTAVEANADDVERALALAIAGAE------KWQKTAPSERAGILLNAAELFQQHAPEF 643 Query: 89 AQMQRLDNGKPISETRALVASAAATFQFFAAACETLEETLTPMRGDNLTMSLYEPMGVVA 148 + + GK + + ++++A T + + + +G+VA Sbjct: 644 IALSVREAGKTLPNAIGELRETIDFLRYYSAQITTAQ--------------MEQALGLVA 689 Query: 149 AITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELARICIEAGVPAGMISVLPG 208 I+PWN P++ +++ ALAAGNAV+ KPAE TPL A ++ +AGVP ++ LPG Sbjct: 690 CISPWNFPLSIFTGQLSAALAAGNAVLAKPAEQTPLIAFRAVQLMHQAGVPEDVLLFLPG 749 Query: 209 KGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMM------PVSLELGGKSPTMVF 262 +G G + V V FTG T I A + M P+ E GG++ +V Sbjct: 750 RGQTTGAQLVADARVSGVVFTGSTQVATIIHRTLAQRSMAERRDIPLIAETGGQNAMIVD 809 Query: 263 EDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAAAALRVGDPADAR 322 A + V V+ F S+G+ C A L + + + + L A L+ G P Sbjct: 810 SSALCEQVVRDVIASAFDSAGQRCSALRVLCLQTDIAEKTLNMLHGAFEELQTGRPDQLA 869 Query: 323 TQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGYYYTPTIIEGVSNQQR 382 T +GP+I A +Q + Y+E ++ + G Q G + P IIE ++ Sbjct: 870 TDVGPVIDAEAQQHLRAYIEQAGRRAKNMKQLALPSTGTQ---GTFVAPCIIE--IDRMS 924 Query: 383 ICQEEIFGPVLVAMPF--DDEDDLIAQANDSVYALAAGIWTRDYKRAWRIGRAVQAGNVW 440 Q E+FGPVL + + DD L+ N + + L G+ +R + I + GN++ Sbjct: 925 DLQREVFGPVLHVLRYRRDDLPQLVRDINATGFGLTLGVHSRIDETINYIVDHARVGNIY 984 Query: 441 INTYKQFSI--STPFGGWRDSGLG----------REKGRLGILQYMEQK 477 +N ++ PFGG SG G R + +G ++ ME K Sbjct: 985 VNRNIIGAVVGVQPFGGEGLSGTGPKAGGPLYMKRLQQEVGAIRNMENK 1033 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 977,260 Number of extensions: 42147 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 3 Length of query: 492 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 407 Effective length of database: 720,272 Effective search space: 293150704 Effective search space used: 293150704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)