BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1273.p (591 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093994|ref|YP_001099069.1| dihydrolipoyl dehydrogenase (E3... 939 0.0 gi|134094976|ref|YP_001100051.1| dihydrolipoamide dehydrogenase ... 279 3e-76 gi|134093993|ref|YP_001099068.1| dihydrolipoyllysine-residue ace... 130 1e-31 gi|134096501|ref|YP_001101576.1| dihydrolipoamide dehydrogenase ... 110 2e-25 >gi|134093994|ref|YP_001099069.1| dihydrolipoyl dehydrogenase (E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes) (dihydrolipoamide dehydrogenase) [Herminiimonas arsenicoxydans] Length = 611 Score = 939 bits (2427), Expect = 0.0, Method: Compositional matrix adjust. Identities = 497/611 (81%), Positives = 539/611 (88%), Gaps = 20/611 (3%) Query: 1 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEVK 60 MS EVKVPDIGDFKEVE+IE++V VGDTIKVDQSLITVESDKASMEIPSS AGVVKE+K Sbjct: 1 MSTIEVKVPDIGDFKEVEIIEMLVNVGDTIKVDQSLITVESDKASMEIPSSHAGVVKELK 60 Query: 61 VKVGDKVAEGSLLLLVEAAGEAAAAPAPAAAPAPAAAAPAPAAAPAAAPTAA-------- 112 VK+GDKVAEGSLLL++EA+ AAA A AAPAPAA AP P A A A + Sbjct: 61 VKLGDKVAEGSLLLILEASESAAAPAAAPAAPAPAAVAPTPEPAAAPAAASVKAAAPVSA 120 Query: 113 ------------SFGGSADVQCDVMVLGGGPGGYSAAFRAADLGLNTVIVERESTLGGVC 160 SF G AD++CD+MVLG GPGGYSAAFR+ADLG+ TV++ER STLGGVC Sbjct: 121 PAAAPVNAPAAASFAGKADIECDMMVLGAGPGGYSAAFRSADLGMATVLIERYSTLGGVC 180 Query: 161 LNVGCIPSKALLHVAAVIDETAAMASHGVTFGKPEIDIDKLRAYKDKVIGTMTGGLAGMA 220 LNVGCIPSKALLHVAAVIDE AAM +HGVTFGKP+IDID+LR YK+KVIG MT GL+GMA Sbjct: 181 LNVGCIPSKALLHVAAVIDEAAAMTAHGVTFGKPQIDIDQLRGYKEKVIGKMTTGLSGMA 240 Query: 221 KARKVQVVNGDGQFAGPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPRIVDS 280 KARKV VV G GQF G NHIEVTAADGSKK +QFK AIIAAGSSVVNLPF+P+DPRI+DS Sbjct: 241 KARKVNVVQGVGQFVGTNHIEVTAADGSKKTIQFKQAIIAAGSSVVNLPFIPEDPRIIDS 300 Query: 281 TGALELRQVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKF 340 TGALELR VPKRMLVIGGGIIGLEMATVYSTLGARIDVVEM+DGLMQGADRDMVKVWQKF Sbjct: 301 TGALELRSVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMLDGLMQGADRDMVKVWQKF 360 Query: 341 NEKRFDNVMVKTKTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLS 400 NEKRFD +M+KTKTVAVEAL EGIKV+FEAAEAG TAPEPQ+YD+VLVAVGRSPNGKK++ Sbjct: 361 NEKRFDKIMLKTKTVAVEALKEGIKVSFEAAEAGVTAPEPQVYDMVLVAVGRSPNGKKIA 420 Query: 401 ADKAGVIVSDRGFIAVDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSF 460 ADKAGV V+DRGFI VD QMRTNV HIFAIGDLVGQPMLAHKAVHE HVAAEA AGEKS+ Sbjct: 421 ADKAGVAVTDRGFIEVDAQMRTNVHHIFAIGDLVGQPMLAHKAVHEAHVAAEAAAGEKSY 480 Query: 461 FDASVIPSVAYTDPEVAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLF 520 FDA VIPSVAYTDPEVAWVG+TEDEAKAKGIK+EKGHFPWAASGRAVANGR EGFTKLLF Sbjct: 481 FDARVIPSVAYTDPEVAWVGITEDEAKAKGIKLEKGHFPWAASGRAVANGRDEGFTKLLF 540 Query: 521 DAETHRIIGGGIVGTHAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAEVAEG 580 DAET RI+GGGIVGTHAGDMIGE+ALAIEMGADAVDIGKTIHPHPTLGES+GMAAEV +G Sbjct: 541 DAETKRIVGGGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEVYKG 600 Query: 581 HCTDLPPQRKK 591 CTDLPP +KK Sbjct: 601 VCTDLPPMKKK 611 >gi|134094976|ref|YP_001100051.1| dihydrolipoamide dehydrogenase [Herminiimonas arsenicoxydans] Length = 475 Score = 279 bits (713), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 170/460 (36%), Positives = 255/460 (55%), Gaps = 13/460 (2%) Query: 136 AAFRAADLGLNTVIVERESTL------GGVCLNVGCIPSKALLHVAAVIDETA-AMASHG 188 AA RAA LG + ++ GG C NVGCIPSKALL + + A A HG Sbjct: 19 AAIRAAQLGFSVACIDEWKNAKGGAAPGGTCTNVGCIPSKALLQSSEHYEHAGHAFAEHG 78 Query: 189 VTFGKPEIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFA--GPNHIEVTAAD 246 + +++ K+ A KD V+ G+ + K KV +G G FA G E+ A Sbjct: 79 IEVKDLTLNVKKMVARKDTVVKQNNDGILYLLKKNKVTFFHGRGSFAKAGAGGYEIKVAG 138 Query: 247 GSKKVVQFKHAIIAAGSSVVNLPFVPQDPR-IVDSTGALELRQVPKRMLVIGGGIIGLEM 305 ++ + KH I+A GS+ LP D + I+ +TGAL + +VPKR+ +IG G+IGLEM Sbjct: 139 AGEETIATKHVIVATGSTPRVLPGADFDEKMILSNTGALAMTEVPKRLGLIGAGVIGLEM 198 Query: 306 ATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTVAVEALPEGIK 365 +V+ LG+ + V+E + + D + K QK K+ + + K A+ + Sbjct: 199 GSVWRRLGSEVTVLEALPVFLGAVDEQVAKEAQKQFVKQGLAINLGVKIGAIAVGKNEVT 258 Query: 366 VTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIAVDKQMRTNVP 425 V + + A + ++D ++V++GR PN L+ + G+ + +RGFIAVD +T++P Sbjct: 259 VNYVDDKGAA---QKAVFDKLIVSIGRIPNTVGLNTEAVGLKLDERGFIAVDGDCKTSLP 315 Query: 426 HIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYTDPEVAWVGVTEDE 485 +++A+GD+V PMLAHKA EG AE IAG+ + + IPSV YT PE+A VG TE + Sbjct: 316 NVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPSVIYTSPEIASVGKTEQQ 375 Query: 486 AKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGIVGTHAGDMIGEVA 545 KA+ I + G FP+ A+GRA A G + GF K L DA+T I+G I+G A ++I E Sbjct: 376 LKAENIAYKAGTFPFLANGRARALGDTTGFVKFLADAKTDEILGVHIIGPQASELISEAV 435 Query: 546 LAIEMGADAVDIGKTIHPHPTLGESLGMAAEVAEGHCTDL 585 +A+E A + DI + H HPTL E+L AA +G + Sbjct: 436 VAMEFRASSEDIARICHAHPTLSEALKEAALAVDGRSLNF 475 >gi|134093993|ref|YP_001099068.1| dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (E2) (dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) [Herminiimonas arsenicoxydans] Length = 455 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 66/76 (86%), Positives = 72/76 (94%) Query: 1 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEVK 60 MS EVKVPDIGDFKEVE+IE++VKVGDTIK+DQSLITVESDKASMEIPSS AGVVKE+K Sbjct: 1 MSTVEVKVPDIGDFKEVEIIELLVKVGDTIKIDQSLITVESDKASMEIPSSHAGVVKEIK 60 Query: 61 VKVGDKVAEGSLLLLV 76 VK+GDKVAEGSLLL V Sbjct: 61 VKLGDKVAEGSLLLTV 76 >gi|134096501|ref|YP_001101576.1| dihydrolipoamide dehydrogenase [Herminiimonas arsenicoxydans] Length = 477 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 125/495 (25%), Positives = 207/495 (41%), Gaps = 50/495 (10%) Query: 120 VQCDVMVLGGGPGGYSAAFRAADLGLNTVIVERESTLGGVCLNVGCIPSKALLHVAAVID 179 ++ DV V+G G G SA +RAA V + G C VGC+PSK L+ A Sbjct: 4 LEFDVAVIGAGSAGMSA-YRAAKTHGKRVALIESGPYGTTCARVGCMPSKLLIAAAEAAH 62 Query: 180 ETAAMASHGVTFGKPEID----IDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFA 235 GV G ID + ++R+ +D+ +G + G+ + A +++ Sbjct: 63 AVGEAPGFGVHAGALRIDGKAVMQRVRSERDRFVGFVVQGIEHIDPADRLR--------- 113 Query: 236 GPNHIEVTAAD----GSKKVVQFKHAIIAAGSSVVNLPFVPQD-----PRIVDSTGALEL 286 +H AAD G VQ + +IA+GSS P +P + +++ S + Sbjct: 114 --SHARFLAADCLQVGEHTQVQAQCVVIASGSS----PQIPPELSGLGKQLIVSDDVFDW 167 Query: 287 RQVPKRMLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFD 346 +P + VIG G+IGLE+ LG R+ + + Q +D + + D Sbjct: 168 DDLPASVAVIGTGVIGLELGQALHRLGVRVAIFGRKARVAQLSDPAVNQTAIACLGAELD 227 Query: 347 NVMVKTKTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGV 406 ++ T V A G +V A + + VL A GR+PN + L + G+ Sbjct: 228 ---LRLNTTIVTARLSGAQVELHTRSADGDE-RTEHFQYVLAATGRTPNVRGLGLETTGL 283 Query: 407 IVSDRGFIAVD-KQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASV 465 ++++G D M+ IF GD+ L H+A EG +A + AG A + Sbjct: 284 QLNEQGLPTFDPSTMQCGDSAIFIAGDVDNDRPLLHEASDEGRIAGDN-AGRFPAVAAGL 342 Query: 466 --IP-SVAYTDPEVAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDA 522 +P ++A+TDP++A +G T K KG + G + GR+ ++ G ++ + Sbjct: 343 RRVPLAIAFTDPQIAMIGHTPRTLK-KGC-FQSGEVSFEDQGRSRVMRQNRGLLRVYGEP 400 Query: 523 ETHRIIGGGIVGTHAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAE------ 576 + R +G + G A + +A A + G + HP + E L A Sbjct: 401 GSLRFLGAEMTGPRAEHLAHLLAWACQSGMTVDQMLDMPFYHPVVEEGLRTALRNLKENL 460 Query: 577 ----VAEGHCTDLPP 587 A HC D P Sbjct: 461 LQHVSAIEHCADCTP 475 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.316 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,007,183 Number of extensions: 42176 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 5 Length of query: 591 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 504 Effective length of database: 713,682 Effective search space: 359695728 Effective search space used: 359695728 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)