BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1257.p (477 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrog... 367 e-103 gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde deh... 164 6e-42 gi|134095327|ref|YP_001100402.1| multifunctional: transcriptiona... 127 1e-30 >gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrogenase I, NADP-dependent [Herminiimonas arsenicoxydans] Length = 492 Score = 367 bits (941), Expect = e-103, Method: Compositional matrix adjust. Identities = 192/471 (40%), Positives = 285/471 (60%), Gaps = 9/471 (1%) Query: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRSDLDRALEAAKKGFETWRNMSAFERS 67 +I+G W +A+ D+ NPA +IG + + + A+ AA+ F W + ER+ Sbjct: 22 YIDGTWAGAAT--NFDISNPADGSIIGSAPNMGAEETETAIRAAQSAFPAWAAKTGKERA 79 Query: 68 KIMRRAADLLRERAEDVARLMTQEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 IMR+ DL+ +D+A LMT EQGKPL E++ E + A I+WF+EEA+R G ++ + Sbjct: 80 IIMRQWFDLIIANVDDLAILMTAEQGKPLIESRGEVIYGASFIEWFSEEAKRVSGDVMAS 139 Query: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPINQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 + IK+P+G AA TPWNFPI + RK++ A+AAGC+I++K E+TP S + Sbjct: 140 TWNDKRTLTIKQPIGICAAITPWNFPIAMITRKVAPAVAAGCTIVIKPAEQTPLSALAIA 199 Query: 188 RAYADAGVPAGVINLVYGVPAE----ISEYLIPHPVIKKISFTGSTPVGKQLAALAGQYM 243 AG+PAGVIN++ AE + + L P+++ +SFTGSTPVG+ L + Sbjct: 200 ELAHRAGLPAGVINVITA-DAENSIVVGKTLCASPIVRHVSFTGSTPVGRILMEQCAPTV 258 Query: 244 KRATMELGGHAPAMVFDDVDVDVAAKTLAMAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 K+ +ELGGHAP +VFDD D+D A +K+RNAGQ CV RF V + +++ FV K Sbjct: 259 KKIALELGGHAPFIVFDDADLDAAVTGALQSKYRNAGQTCVCTNRFYVHESIYDVFVQKL 318 Query: 304 VTYAEQLKVGNGLEAGVNMGPVANKRRIPALEALIDDALAQGAQLRTGGKRIGNKGYFFQ 363 A ++KVGNG E GV GP+ + + I +E + DA+ +GA + TGGK+ G+F++ Sbjct: 319 AAGAARIKVGNGFEQGVLQGPLIDDQAIRKVEQHVSDAVKKGASVMTGGKKHALGGHFYE 378 Query: 364 PTVLTNIPLNARVMNEEPFGPLALINSFESLDDVIAEANRLPFGLAAYAFAKSAHTKHAL 423 PTVL+NI + +M+EE FGP+A I F++ D+ IA AN FGLA+Y +A+ + Sbjct: 379 PTVLSNITDDMSIMHEETFGPVAAIVKFKTEDEAIAAANNTDFGLASYFYARDISRVWRV 438 Query: 424 AARVESGMLTINHLGLALPEL-PFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 A ++E GM+ IN GL E+ PFGG+K SG G EG I+ Y+ K + Sbjct: 439 AEKLEYGMVGIN-TGLISNEVAPFGGVKQSGLGREGSKYGIDEYLEIKYLC 488 >gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde dehydrogenase, oxidoreductase protein [Herminiimonas arsenicoxydans] Length = 506 Score = 164 bits (416), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 131/481 (27%), Positives = 218/481 (45%), Gaps = 12/481 (2%) Query: 5 VQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRSDLDRALEAAKKGFETWRNMSAF 64 ++ +ING T + G V NPAT E+ + A +D+ A+ AAK W M+ Sbjct: 9 IEHYINGNLTNTVGGGQQPVFNPATGEISANVVLASDADVQTAVAAAKAAAPAWAEMAPL 68 Query: 65 ERSKIMRRAADLLRERAEDVARLMTQEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRL 124 +R++I+ + DL+ D+A +T+E GK ++AK E + +++ + + Sbjct: 69 KRARILFKFKDLIERHHNDLAAAITREHGKVFSDAKGEVTRGLEVVEFACGVPQLLKTQF 128 Query: 125 VPARVPGAYQMVIKEPVGPVAAFTPWNFPINQVVRKLSAALAAGCSIIVKAPEETPASPA 184 G +++P+G A TP+NFP + ALA G + I+K E P+ Sbjct: 129 SDNIGGGIDNWNLRQPLGVTAGITPFNFPFMVPMWMAPVALATGNTFILKPSERDPSPSL 188 Query: 185 ELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQL----AALAG 240 + AG+P GV N++ G + + L+ HP IK +SF GSTP+ + + AA G Sbjct: 189 MVAELLHQAGLPDGVFNVLQGDKRAV-DGLLQHPDIKAVSFVGSTPIAEYIYQAGAARKG 247 Query: 241 QYMKRATMELGGHAPAMVFDDVDVDVAAKTLAMAKFRNAGQVCVSPTRFLVQKGVFNAFV 300 Y R G +V D +++ A L A + +AG+ C++ + + V + V Sbjct: 248 SYPLRVQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLV 307 Query: 301 DKFVTYAEQLKVGNGLEAGVNMGPVANKRRIPALEALIDDALAQGAQLRTGGKRI----G 356 + + LKV NG+E+ MGPV + +E I+ + GA+L G+ + Sbjct: 308 AALIPRVKSLKVKNGMESDAEMGPVVSAAHKAKIEGYIEAGIKSGAKLLVDGRGLTVDGH 367 Query: 357 NKGYFFQPTVLTNIPLNARVMNEEPFGPLALINSFESLDDVIAEANRLPFGLAAYAFAKS 416 + G+F + N+ + EE FGP+ + + N FG F Sbjct: 368 SNGFFLGGCLFDNVTPEMTIYQEEIFGPVLCVVRVPDFSAALDLINAHEFGNGVALFTSD 427 Query: 417 AHTKHALAARVESGMLTIN-HLGLALPELPFGGIKDSGYGSEG--GTEAIEAYINTKLVT 473 +T + RVE GM+ IN + + + FGG K S +G G E I+ Y K + Sbjct: 428 GNTAREFSRRVEIGMVGINVPIPVPMAWHSFGGWKRSMFGDTHAYGEEGIKFYTRYKSIM 487 Query: 474 Q 474 Q Sbjct: 488 Q 488 >gi|134095327|ref|YP_001100402.1| multifunctional: transcriptional repressor of proline utilization; proline dehydrogenase; pyrroline-5-carboxylate dehydrogenase [Herminiimonas arsenicoxydans] Length = 1224 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 119/461 (25%), Positives = 208/461 (45%), Gaps = 38/461 (8%) Query: 16 SASGRTIDVI-NPATE-EVIGKIAHADRSDLDRALEAAKKGFETWRNMSAFERSKIMRRA 73 +AS T+ ++ NPA +V+G A+ D++RAL A G E W+ + ER+ I+ A Sbjct: 573 NASSTTVAIMRNPADHSDVLGTAVEANADDVERALALAIAGAEKWQKTAPSERAGILLNA 632 Query: 74 ADLLRERAEDVARLMTQEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPARVPGAY 133 A+L ++ A + L +E GK L A E +TID+ R A++ A Sbjct: 633 AELFQQHAPEFIALSVREAGKTLPNAIGEL---RETIDFL---------RYYSAQITTA- 679 Query: 134 QMVIKEPVGPVAAFTPWNFPINQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYADA 193 QM ++ +G VA +PWNFP++ +LSAALAAG +++ K E+TP ++ A Sbjct: 680 QM--EQALGLVACISPWNFPLSIFTGQLSAALAAGNAVLAKPAEQTPLIAFRAVQLMHQA 737 Query: 194 GVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQYMKRATM----- 248 GVP V+ + G L+ + + FTGST Q+A + + + + +M Sbjct: 738 GVPEDVLLFLPGRGQTTGAQLVADARVSGVVFTGST----QVATIIHRTLAQRSMAERRD 793 Query: 249 -----ELGGHAPAMVFDDVDVDVAAKTLAMAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 E GG +V + + + + F +AGQ C + +Q + ++ Sbjct: 794 IPLIAETGGQNAMIVDSSALCEQVVRDVIASAFDSAGQRCSALRVLCLQTDIAEKTLNML 853 Query: 304 VTYAEQLKVGNGLEAGVNMGPVANKRRIPALEALIDDALAQGAQLRT-GGKRIGNKGYFF 362 E+L+ G + ++GPV + L A I+ A + ++ G +G F Sbjct: 854 HGAFEELQTGRPDQLATDVGPVIDAEAQQHLRAYIEQAGRRAKNMKQLALPSTGTQGTFV 913 Query: 363 QPTVLTNIPLNARVMNEEPFGPLALINSF--ESLDDVIAEANRLPFGLAAYAFAKSAHTK 420 P ++ ++ + E FGP+ + + + L ++ + N FGL ++ T Sbjct: 914 APCIIEIDRMSD--LQREVFGPVLHVLRYRRDDLPQLVRDINATGFGLTLGVHSRIDETI 971 Query: 421 HALAARVESGMLTINH--LGLALPELPFGGIKDSGYGSEGG 459 + + G + +N +G + PFGG SG G + G Sbjct: 972 NYIVDHARVGNIYVNRNIIGAVVGVQPFGGEGLSGTGPKAG 1012 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 874,398 Number of extensions: 36900 Number of successful extensions: 116 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 3 Length of query: 477 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 392 Effective length of database: 720,272 Effective search space: 282346624 Effective search space used: 282346624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)