BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1208.p (418 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096423|ref|YP_001101498.1| putative isocitrate dehydrogena... 184 8e-48 gi|134096382|ref|YP_001101457.1| tartrate dehydrogenase [Hermini... 122 3e-29 gi|134094446|ref|YP_001099521.1| 3-isopropylmalate dehydrogenase... 89 5e-19 >gi|134096423|ref|YP_001101498.1| putative isocitrate dehydrogenase [NAD] subunit alpha [Herminiimonas arsenicoxydans] Length = 348 Score = 184 bits (466), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 124/388 (31%), Positives = 194/388 (50%), Gaps = 55/388 (14%) Query: 31 IPFIMGDGVGVDVTPVMLKVVDAAVAKAYGGARKIHWMEIYAGEKATKLYGQDVWLPDET 90 + I GDG+G ++ V+++V DA W AG A + G LP T Sbjct: 12 VTLIPGDGIGPEIVDVVVRVFDAL-------GNPFAWETQQAGVNALEKSGD--LLPQVT 62 Query: 91 LEVLKQYLVSIKGPLSTPVGGGIRSLNVTMRQQLDLYVCLRPVRYFKGVPSPLREPEKTD 150 L+ + + +++KGPLSTP+GGG RS+NV +R+ LY +RP R P EK D Sbjct: 63 LDSITRTGLALKGPLSTPIGGGFRSVNVRLRETFQLYANVRPARTI----VPGGRYEKID 118 Query: 151 MVIFRENSEDIYAGIEWQAGTPEVKKLIELLTREMGVKKLRFPETSALGIKPVSREGTER 210 +V+ REN E +Y G E + + + T GI +R G+ R Sbjct: 119 LVLVRENLEGLYVGHEHYVPIGDDAHAVAMAT----------------GIN--TRAGSRR 160 Query: 211 LVRKAIQYAIDHDKPSVTLVHKGNIMKYTEGAFRDWGYALAGREFGAELIDDGPWMRLKN 270 + + A YA+ +++ VT+VHK N++K G F + ++ G + Sbjct: 161 ISKFAFDYAVRNNRKKVTIVHKANVLKALTGLFLE-----TAKQVGLNYAN--------- 206 Query: 271 PKTGRQIIIKDAITDAFFQQVLMRPAEYSVIATMNLNGDYISDAVAAQVGGIGIAPGANM 330 Q+ D I DA Q+++ P ++ VI + NL GD +SD +A VGG+G+APGAN+ Sbjct: 207 -----QVEFDDRIVDACAMQLVLNPWQFDVIVSTNLFGDILSDQIAGLVGGLGMAPGANI 261 Query: 331 SDSVAVFEATHGTAPKYAGKDYVNPGSIILSAEMMLRHMGWIEAADLIITAMQKSVSAKR 390 + A+FEA HG+AP AGK NP +++L+A +ML H G A L+ A+ +S + Sbjct: 262 GERTAIFEAVHGSAPDIAGKGMANPTALLLAAALMLEHKGLNTQAGLLRDAIDSVLSTES 321 Query: 391 VTYDFARLLEGATQVSCSGFGEVMIENM 418 R + VS S +G+++IE + Sbjct: 322 -----GRTFDLGGTVSTSKYGDLLIERI 344 >gi|134096382|ref|YP_001101457.1| tartrate dehydrogenase [Herminiimonas arsenicoxydans] Length = 356 Score = 122 bits (306), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 106/378 (28%), Positives = 173/378 (45%), Gaps = 60/378 (15%) Query: 31 IPFIMGDGVGVDVTPVMLKVVDAAVAKAYGGARKIHWMEIYAGEKATKLYGQD-VWLPDE 89 I I GDG+G +V P L+V+DAA AK +G IH + + Y Q +PD+ Sbjct: 7 IAIIPGDGIGKEVIPEGLRVLDAA-AKRFG----IHLEYKHIEWASCDYYAQTGKMMPDD 61 Query: 90 TLEVLKQ----YLVSIKGPLSTPVGGGIRSLNVTMRQQLDLYVCLRPVRYFKGVPSPL-- 143 + L+ Y ++ P + P + + R++ D YV LRPVR F+GVP PL Sbjct: 62 WKQQLQGCDAIYFGAVGWPATVPDHVSLWGSLLKFRREFDQYVNLRPVRLFEGVPCPLAN 121 Query: 144 REPEKTDMVIFRENSEDIYAGIEWQAGTPEVKKLIELLTREMGVKKLRFPETSALGIKPV 203 R+P D + REN+E Y I G + E RE+ +++ F Sbjct: 122 RKPGDIDFYVVRENTEGEYTAI---GGI-----MYEGTERELVLQQSAF----------- 162 Query: 204 SREGTERLVRKAIQYAIDHDKPSVTLVHKGNIMKYTEGAFRDWGYALAGREFGAELIDDG 263 SR GT+R+++ A + A + +T+ K N G A++ Sbjct: 163 SRRGTDRILKYAFELAQSRARRRLTVATKSN------------GIAISM----------- 199 Query: 264 PWMRLKNPKTGRQ---IIIKDAITDAFFQQVLMRPAEYSVIATMNLNGDYISDAVAAQVG 320 PW + + + I + D + +++P + V+ NL GD +SD A G Sbjct: 200 PWWDSRADEMAKNYPDITVDKQHIDILAARFVLQPDRFDVVVASNLFGDILSDLGPACTG 259 Query: 321 GIGIAPGANMSDSV---AVFEATHGTAPKYAGKDYVNPGSIILSAEMMLRHMGWIEAADL 377 +G+AP AN++ ++FE HG+AP G++ NP ++I S +ML +G A D Sbjct: 260 TMGLAPSANLNPERLFPSLFEPVHGSAPDIYGQNIANPIAMIWSGALMLDFLGQRAAHDA 319 Query: 378 IITAMQKSVSAKRVTYDF 395 I+ A+++ ++ T D Sbjct: 320 ILEAIKQVLAHGPRTPDL 337 >gi|134094446|ref|YP_001099521.1| 3-isopropylmalate dehydrogenase [Herminiimonas arsenicoxydans] Length = 356 Score = 88.6 bits (218), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 108/400 (27%), Positives = 176/400 (44%), Gaps = 59/400 (14%) Query: 31 IPFIMGDGVGVDVTPVMLKVVDAAVAKAYGGARKIHWMEIYAGEKATKLYGQDVWLPDET 90 I + GDG+G ++ +KV++A K + G A G LP+ T Sbjct: 3 IAILPGDGIGPEIMNQAVKVMNAL-------GEKFETETVPVGGAAYAEQGHP--LPEST 53 Query: 91 LEVLKQYLVSIKGPLSTPVGGGI-RSLN-----VTMRQQLDLYVCLRP-VRYFKGVPSPL 143 L+V K+ + G + + RSL + +R+ L L+ RP + Y + + Sbjct: 54 LKVAKEADAILFGAVGDFKYDTLERSLRPEQAILGLRKHLGLFANFRPAILYPELAGASS 113 Query: 144 REPE---KTDMVIFRENSEDIYAGIEWQAGTPEVKKLIELLTREMGVKKLRFPETSALGI 200 +PE D++I RE + DIY G G E RE G +R+ E Sbjct: 114 LKPEIVSGLDIMIVRELTGDIYFG--QPRGMREAPDGPFKGARE-GFDTMRYSEPE---- 166 Query: 201 KPVSREGTERLVRKAIQYAIDHDKPSVTLVHKGNIMKYTEGAFRDWGYALAGREFGAELI 260 R+ A Q A K +T V K N+++ F+ W +++ Sbjct: 167 -------IRRIAHVAFQAAQKRSK-RLTSVDKANVLE----TFQFW----------RDVV 204 Query: 261 DDGPWMRLKNPKTGRQIIIKDAITDAFFQQVLMRPAEYSVIATMNLNGDYISDAVAAQVG 320 D K + + + D Q++ P E+ V+ T N+ GD +SDA A G Sbjct: 205 TD-------VHKEYKDVKLDHMYVDNAAMQLVRAPKEFDVVVTGNMFGDILSDAAAMLTG 257 Query: 321 GIGIAPGANM-SDSVAVFEATHGTAPKYAGKDYVNPGSIILSAEMMLRH-MGWIEAADLI 378 IG+ P A++ +++ ++E +HG+AP AGKD NP + ILS MMLR+ +G E AD I Sbjct: 258 SIGMLPSASLDANNKGLYEPSHGSAPDIAGKDIANPLAQILSLAMMLRYSLGKTEQADRI 317 Query: 379 ITAMQKSVSAKRVTYDFARLLEGATQVSCSGFGEVMIENM 418 TA++ + T D G T+VS + G+ +++ + Sbjct: 318 ETAVKAVLKQGLRTVDIYE--AGTTKVSTTQMGDAVVKAL 355 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 790,044 Number of extensions: 32472 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 96 Number of HSP's successfully gapped: 4 Length of query: 418 Length of database: 1,000,347 Length adjustment: 84 Effective length of query: 334 Effective length of database: 723,567 Effective search space: 241671378 Effective search space used: 241671378 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)