BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0890.p (345 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldeh... 77 2e-15 gi|134095240|ref|YP_001100315.1| glutathione-independent formald... 67 9e-13 gi|134094301|ref|YP_001099376.1| putative glutathione-dependent ... 63 2e-11 >gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 369 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 84/351 (23%), Positives = 143/351 (40%), Gaps = 42/351 (11%) Query: 13 LRIEPLDTGALQPQQLKVKVKAGGICGSDLHYYH----HGGFGVVRIKEPMVLGHEVSGQ 68 L I +D + ++ +K+ G+C +D G F P+VLGHE +G Sbjct: 15 LEIVEIDVEPPRKGEVLIKITNTGVCHTDAFTLSGDDPEGLF-------PVVLGHEGAGI 67 Query: 69 IAEVGSAISDIAPGTRIAISPSRPCGLCKYCQEGKQNHCL--------------DMRFYG 114 + EVG ++ + PG + + CG C +C+ GK N C+ RF Sbjct: 68 VVEVGEGVTSVKPGDHVIPLYTAECGECLFCKSGKTNLCVAVRATQGKGLMPDGTTRFSY 127 Query: 115 SAMRTPHVQG--AFRQEIII-ERSQAHIVADHVSDGEAAMAEPLSVALHAVRRAGPLV-G 170 + H G F + ++ E S A I D + + ++ + AV + G Sbjct: 128 NGQPLYHYMGCSTFSEYTVVAEVSLAKINPDANPEHVCLLGCGVTTGIGAVHNTAKVQPG 187 Query: 171 KRVLVTGCGPIGALVVVAARRAGALEVVVTDVARMPLETALKVGADRAINMAETPDALKA 230 V + G G IG + AR+A A ++ D E A GA +N + + Sbjct: 188 DSVAIFGLGGIGLAAIQGARQAKAGRIIAIDTNPAKFELARTFGATECLNPKDFDKPIHE 247 Query: 231 YAADKGTF--DVLFEASGNERALVGALEALR---PQAIIVQV-GLGGDIQF-PANLLVGK 283 + + D FE GN + ALEA Q+I++ V G G +I P L+ G+ Sbjct: 248 VLIEMTGWGVDHTFECIGNVNVMRSALEAAHRGWGQSIVIGVAGAGKEISTRPFQLVTGR 307 Query: 284 ELDWRGA----FRFHEEFAMAVELMNRKLVDVKPLISATYPISQAVDAFEI 330 W+G+ + + VE + +D+ P ++ T + + AF++ Sbjct: 308 --TWKGSAFGGVKGRTQLPGMVEDAMKGEIDLAPFVTHTMGLDEINHAFDL 356 >gi|134095240|ref|YP_001100315.1| glutathione-independent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 414 Score = 67.4 bits (163), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 59/226 (26%), Positives = 100/226 (44%), Gaps = 34/226 (15%) Query: 21 GALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEVGSAISDIA 80 G P +K+ ICGSDLH Y G + + M +GHE++G++ EVGS + + Sbjct: 42 GKSAPHGAILKIVTTNICGSDLHIYR----GSFAVPKGMTMGHEMTGEVIEVGSDVEVVK 97 Query: 81 PGTRIAISPSRPCGLCKYCQEGKQNHC------LDMRFYGSAMRTPHVQGAFRQEIIIER 134 G +++ + CG C C+ + + C +D YG + G + + Sbjct: 98 KGDIVSVPFNVGCGRCYNCKHMRSDVCENTNPEIDCGAYGFNLGG--WTGGQGDYLFVPY 155 Query: 135 SQAHIVADHVSDGEAAM---------AEPLSVALHAVRRAGP--------LVGKRVLVTG 177 + +++ D +AAM ++ L A H AGP +VG+ +L+ G Sbjct: 156 ADFNLL--RFPDKDAAMEKIRDLTLLSDVLPTAFHGF--AGPDWPAAPAYVVGENILIFG 211 Query: 178 CGPIGALVVVAARRAGALEVVVTDVARMPLETALKVGADRAINMAE 223 GP+G AR GA ++V D + L+ LK IN+++ Sbjct: 212 AGPVGRAGAACARLLGAGAIIVADYIQERLDL-LKPHGVETINLSD 256 >gi|134094301|ref|YP_001099376.1| putative glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 368 Score = 63.2 bits (152), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 93/350 (26%), Positives = 149/350 (42%), Gaps = 33/350 (9%) Query: 9 APHDLRIEPLDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQ 68 A L IE ++ G + ++ V++KA GIC +D YY G I P +LGHE +G Sbjct: 11 AGQPLTIEEVELGGPREGEVLVEIKATGICHTD--YYTLSGADPEGIF-PAILGHEGAGV 67 Query: 69 IAEVGSAISDIAPGTRIAISPSRPCGLCKYCQEGKQNHCLDMRFYGSAMRTP-------- 120 + EVG+ + + + + C CK+C K N C +R P Sbjct: 68 VVEVGAGVKSLKKDDHVIPLYTPECRECKFCLSRKTNLCQKIRSTQGRGLMPDATSRFSI 127 Query: 121 ------HVQG--AFRQEIII-ERSQAHIVADHVSDGEAAMAEPLSVALHAVRRAGPL-VG 170 H G F I++ E + A I D D + ++ + AV + G Sbjct: 128 DGKPLFHYMGTSTFSNYIVVPEIALAKIREDAPFDKVCYIGCGVTTGVGAVLFTAKVEAG 187 Query: 171 KRVLVTGCGPIGALVVVAARRAGALEVVVTDVARMPLETALKVGADRAINMAETPDALKA 230 V+V G G IG V+ AA+ GA +++ D+ A K G IN ++ + + A Sbjct: 188 ANVVVFGLGGIGLNVIQAAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSKVENVVDA 247 Query: 231 YAA-DKGTFDVLFEASGNERALVGALEALRP---QAIIVQVG-LGGDIQF-PANLLVGKE 284 G D FE GN + ALE Q+I++ V G +I P L+ G+E Sbjct: 248 IVQLTDGGADYSFECIGNVHTMRQALECCHKGWGQSIVIGVAEAGAEISTRPFQLVTGRE 307 Query: 285 LDWRG-AF---RFHEEFAMAVELMNRKLVDVKPLISATYPISQAVDAFEI 330 W+G AF R + V+ +++ LI+ T P+ + + F++ Sbjct: 308 --WKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHTLPLERINEGFDL 355 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.322 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 624,710 Number of extensions: 24596 Number of successful extensions: 87 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 85 Number of HSP's successfully gapped: 3 Length of query: 345 Length of database: 1,000,347 Length adjustment: 82 Effective length of query: 263 Effective length of database: 730,157 Effective search space: 192031291 Effective search space used: 192031291 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)