BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0833.p (585 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093980|ref|YP_001099055.1| dihydroxy-acid dehydratase [Her... 277 1e-75 gi|134094317|ref|YP_001099392.1| phosphogluconate dehydratase [H... 198 5e-52 >gi|134093980|ref|YP_001099055.1| dihydroxy-acid dehydratase [Herminiimonas arsenicoxydans] Length = 557 Score = 277 bits (708), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 177/516 (34%), Positives = 292/516 (56%), Gaps = 22/516 (4%) Query: 29 RSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKVAEHVRRGIIEAGGFPVEF--P 86 RS G +F KP++GI N S +TPCN+ +K+A+ + I +AGG P F P Sbjct: 20 RSMYYAMGYEKADFD-KPMVGIANGHSTITPCNSGLQKLADIAIKTIKDAGGNPQVFGTP 78 Query: 87 VFSNGES---NLRPTAMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASCD 143 S+G S ++++R + + +E ++ G +D V+++ GCDK P ++ + Sbjct: 79 TISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVLVIGGCDKNMPGGMIAMLRTN 138 Query: 144 VPAIVVTGGPMLNGKHQGRDIGSGTVV--WQLSEQVKAGEITIHDFMAAEAGMSRSAGTC 201 VP+I V GG + G +G+D+ T+V ++ + AG ++ DF E S+G+C Sbjct: 139 VPSIYVYGGTIKPGHWKGKDL---TIVSAFEAVGEFTAGRMSQEDFDGIERNACPSSGSC 195 Query: 202 NTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMVWEGLTLSKILTR 261 M TA+TM+ E+LG++LP+++ + D + A S +++ + + L I+TR Sbjct: 196 GGMYTANTMSSSFEALGLALPYSSTMANPDDEKVGSAAESARVLIEAIKKDLKPRDIVTR 255 Query: 262 KAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRFLM 321 KA ENA+ A GGSTNAV+H AIA +D ++D+ R+ + P I DL+PSG+++ Sbjct: 256 KAIENAVAVIMATGGSTNAVLHFLAIAHAAEIDWTIDDFERMRKKVPVICDLKPSGKYVA 315 Query: 322 EEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWD---NVKDAPIYNDEVVRPLAKPL 378 + + AGG+P V++ L +A LL H D +T+ GQT+ + ++ P + +V+ + K L Sbjct: 316 TDLHKAGGIPQVMKVLLDAGLL-HGDCMTITGQTVAEALAHIPSVPSADQDVIHTIDKAL 374 Query: 379 IEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASC 438 G + IL+GNL+P G V K + ++ G A VF+D + I + A Sbjct: 375 YAQGHLAILKGNLSPEGCVAKITGLKNPVIT--GPARVFDDEYSAMDAIMAN--QIKAGD 430 Query: 439 VLVMKNVGPKGYPGMAEVGNMGLPPKILATGVKDMV-RISDARMSGTAYGTVILHVAPEA 497 VLVM+ +GPKG PGM E+ + ++ G+ + V I+D R SG +G V+ HV+PEA Sbjct: 431 VLVMRYLGPKGGPGMPEM--LAPTSALVGQGLGESVGLITDGRFSGGTWGMVVGHVSPEA 488 Query: 498 AAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRR 533 GG +G+V++GD + +DA +QL++SEEE+ RR Sbjct: 489 FVGGTIGLVEEGDSVTIDARQLLIQLNVSEEEIARR 524 >gi|134094317|ref|YP_001099392.1| phosphogluconate dehydratase [Herminiimonas arsenicoxydans] Length = 637 Score = 198 bits (504), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 164/523 (31%), Positives = 249/523 (47%), Gaps = 46/523 (8%) Query: 43 QGKPVIGICNTWSELTPCNAHFRKVAEHVRRGIIEAGG---FPVEFPVFSNGESNLRP-- 97 Q P + I + ++++ + + + ++ + AGG F P +G + +P Sbjct: 65 QKNPSVAIISAYNDMLSAHQPYERFPRIIKDAVRAAGGVAQFAGGVPAMCDGITQGQPGM 124 Query: 98 -TAMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASC-DVPAIVVTGGPML 155 ++ +R+ +M ++ N DA V L CDK P LL+GA +PA+ + GGPM Sbjct: 125 ELSLFSRDTIAMSTAIALSHNMFDAAVFLGICDKIVPGLLIGALHFGHLPAVFIPGGPMT 184 Query: 156 NGKHQGRDIGSGTVVWQLSEQVKAGEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAE 215 G + + QL Q G+I + AE+ AGTC GTA++ + E Sbjct: 185 TGMSNSEK----SRIRQLYAQ---GKIDREALLEAESKSYHDAGTCTFYGTANSNQMLME 237 Query: 216 SLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMVWEGLTLS---KILTRKAFENAIRTNA 272 +G+ LP +A + R L + R V++ +G T + +++ K NAI Sbjct: 238 VMGLHLPGSAFVTPNTPLRDALTVRAAQRAVEISAQGQTFTPVGRLVDEKTIVNAIVALL 297 Query: 273 AIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRFLMEEFYYAGGLPA 332 GGSTN IHL AIA G+ ++ D+ ++ P + + P+G + F+ AGG Sbjct: 298 TTGGSTNHTIHLIAIAKAAGIVIDWNDFDQLSAVVPLLTRIYPNGEADVNHFHAAGGTGF 357 Query: 333 VLRRLGEADLLPHKDALTVNG---------------QTMWDNVKDAP-IYNDE-VVRPLA 375 V+R L +A LL H D T+ G Q +W K AP I DE V+RP Sbjct: 358 VIRELLDAGLL-HDDVTTILGEGLRAHCAEPFLDGDQAVW---KPAPHISADESVLRPAD 413 Query: 376 KPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVD 435 +P DGG+ +L GNL AV+K SA PE + A+VF+ + D L+ D Sbjct: 414 RPFAPDGGLRLLTGNLG--RAVIKISAVKPEYHRVEAPAIVFKSQEEFMHAYQDGKLNQD 471 Query: 436 ASCVLVMKNVGPKGYPGMAEVGNMGLPPKILATGVKDMVRISDARMSGTAYGTV--ILHV 493 V V++ GPK GM E+ + L + ++D RMSG A G V +HV Sbjct: 472 --FVAVVRFQGPKAN-GMPELHALTPALGSLQDAGFHVALVTDGRMSG-ASGKVPAAIHV 527 Query: 494 APEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEA 536 +PE AGGPLG ++DGD I LDA AG LQ + E ++R A Sbjct: 528 SPEVLAGGPLGKIRDGDIILLDAEAGILQALVDAAEWQQRPHA 570 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,218,857 Number of extensions: 55160 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 2 Length of query: 585 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 498 Effective length of database: 713,682 Effective search space: 355413636 Effective search space used: 355413636 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)