BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0535.p (386 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095240|ref|YP_001100315.1| glutathione-independent formald... 157 7e-40 gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldeh... 89 4e-19 gi|134094301|ref|YP_001099376.1| putative glutathione-dependent ... 70 2e-13 gi|134095544|ref|YP_001100619.1| zinc-binding alcohol dehydrogen... 65 5e-12 >gi|134095240|ref|YP_001100315.1| glutathione-independent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 414 Score = 157 bits (398), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 130/376 (34%), Positives = 180/376 (47%), Gaps = 59/376 (15%) Query: 29 ILKVSATAICGSDLHLYRGKIPATQAGDILGHEFMGVVVEAGPAVKRLKKGDRVVVPFVI 88 ILK+ T ICGSDLH+YRG A G +GHE G V+E G V+ +KKGD V VPF + Sbjct: 50 ILKIVTTNICGSDLHIYRGSF-AVPKGMTMGHEMTGEVIEVGSDVEVVKKGDIVSVPFNV 108 Query: 89 ACGQCFFCDQHLYAACETTNPDRGAIINGKGIRSGAAMFGYSHLYGGMPGGQAQYVRVPK 148 CG+C+ C CE TNP+ I GA Y GG GGQ Y+ VP Sbjct: 109 GCGRCYNCKHMRSDVCENTNPE---------IDCGA----YGFNLGGWTGGQGDYLFVPY 155 Query: 149 ADVGPIRI-DSALADEQV---LFLSDILPTGYQAVKNAGVGE------GSSVAIYGAGPV 198 AD +R D A E++ LSD+LPT + G ++ I+GAGPV Sbjct: 156 ADFNLLRFPDKDAAMEKIRDLTLLSDVLPTAFHGFAGPDWPAAPAYVVGENILIFGAGPV 215 Query: 199 GQMAAACARMLGAEKIFMVDHHDYRLRFAVEQYDVLPVNF-DQVDPAEYIIANTAGRGVD 257 G+ AACAR+LGA I + D+ RL ++ + V +N D V E++ T R VD Sbjct: 216 GRAGAACARLLGAGAIIVADYIQERLDL-LKPHGVETINLSDGVPIEEHLERITGHREVD 274 Query: 258 AVIDAVGFEAKG------SALETAMTNLKLEGSSGQALRHCIASVRRGGTLSVPGVY--- 308 VID VG + +G +E+A+TN L+ VR GG S GVY Sbjct: 275 RVIDYVGVDCRGFGAEADKIVESAVTNAMLK------------YVRFGGMTSTVGVYCAN 322 Query: 309 -------AGFIHGFL-FGDIFEKGLTVKTGQTHVQHYMPELLASIEDG---WLRPEAIIT 357 A H L + + + K + GQ+ +Y L+ +I + +L P + T Sbjct: 323 PISKDPKAKKGHMDLEWSNAWIKSPRMSAGQSPTANYNHALMRAILNDRMPYLSP-MMNT 381 Query: 358 HRLPLEEAARGYRIFN 373 + LE+A Y+ F+ Sbjct: 382 KFIKLEDAPAAYKEFD 397 >gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 369 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 79/258 (30%), Positives = 115/258 (44%), Gaps = 18/258 (6%) Query: 27 DIILKVSATAICGSDLHLYRGKIPATQAGDILGHEFMGVVVEAGPAVKRLKKGDRVVVPF 86 ++++K++ T +C +D G P +LGHE G+VVE G V +K GD V+ + Sbjct: 29 EVLIKITNTGVCHTDAFTLSGDDPEGLFPVVLGHEGAGIVVEVGEGVTSVKPGDHVIPLY 88 Query: 87 VIACGQCFFCDQHLYAACETTNPDRGAIINGKGIR-SGAAMFGYS--HLYGGMPGGQ-AQ 142 CG+C FC C + GKG+ G F Y+ LY M ++ Sbjct: 89 TAECGECLFCKSGKTNLCVAVRATQ-----GKGLMPDGTTRFSYNGQPLYHYMGCSTFSE 143 Query: 143 YVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVKN-AGVGEGSSVAIYGAGPVGQM 201 Y V A+V +I+ E V L + TG AV N A V G SVAI+G G +G Sbjct: 144 YTVV--AEVSLAKINPDANPEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAIFGLGGIGLA 201 Query: 202 AAACARMLGAEKIFMVDHHDYRLRFAVE---QYDVLPVNFDQVDPAEYIIANTAGRGVDA 258 A AR A +I +D + + A + P +FD+ P ++ G GVD Sbjct: 202 AIQGARQAKAGRIIAIDTNPAKFELARTFGATECLNPKDFDK--PIHEVLIEMTGWGVDH 259 Query: 259 VIDAVG-FEAKGSALETA 275 + +G SALE A Sbjct: 260 TFECIGNVNVMRSALEAA 277 >gi|134094301|ref|YP_001099376.1| putative glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 368 Score = 69.7 bits (169), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 100/367 (27%), Positives = 155/367 (42%), Gaps = 37/367 (10%) Query: 27 DIILKVSATAICGSDLHLYRGKIPATQAGDILGHEFMGVVVEAGPAVKRLKKGDRVVVPF 86 ++++++ AT IC +D + G P ILGHE GVVVE G VK LKK D V+ + Sbjct: 29 EVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVEVGAGVKSLKKDDHVIPLY 88 Query: 87 VIACGQCFFCDQHLYAACET--TNPDRGAIINGKGIRS--GAAMFGYSHLYGGMPGGQAQ 142 C +C FC C+ + RG + + S G +F Y + Sbjct: 89 TPECRECKFCLSRKTNLCQKIRSTQGRGLMPDATSRFSIDGKPLFHYMGT-----STFSN 143 Query: 143 YVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK-NAGVGEGSSVAIYGAGPVGQM 201 Y+ VP+ + IR D+ ++V ++ + TG AV A V G++V ++G G +G Sbjct: 144 YIVVPEIALAKIREDAPF--DKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGIGLN 201 Query: 202 AAACARMLGAEKIFMVDHHDYRLRFAVEQYDVLPVNFDQVDPAEYIIANTAGRGVDAVID 261 A+M+GA+KI VD + R A + +N +V+ I G D + Sbjct: 202 VIQAAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSKVENVVDAIVQLTDGGADYSFE 261 Query: 262 AVG-FEAKGSALETAMTNLKLEGSSGQALRHCIASVRRGGTLSVPG---VYAGFIHGFLF 317 +G ALE GQ++ I G +S V G F Sbjct: 262 CIGNVHTMRQALECCHKGW------GQSI--VIGVAEAGAEISTRPFQLVTGREWKGSAF 313 Query: 318 GDIFEKGLTVKTGQTHVQHYMPELLASIEDGWLRPEAIITHRLPLEEAARGYRIFNEKQE 377 G G+T V P+++ +G L + +ITH LPLE G+ + + E Sbjct: 314 GG--------ARGRTDV----PKIVDWYMEGKLNIDDLITHTLPLERINEGFDLM-KSGE 360 Query: 378 ECRKVVL 384 R VV+ Sbjct: 361 SIRSVVI 367 >gi|134095544|ref|YP_001100619.1| zinc-binding alcohol dehydrogenase [Herminiimonas arsenicoxydans] Length = 277 Score = 65.5 bits (158), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 39/119 (32%), Positives = 66/119 (55%), Gaps = 4/119 (3%) Query: 1 MRALLYHSAHD-VRVESVPDPIIQEADDIILKVSATAICGSDLHLYRGKIPATQAGDILG 59 M+A++ +A + +++ SV P+ A+D++++V +C +DLHL ++P I G Sbjct: 1 MKAMVLSAAGEALQLRSVEKPV-AGANDLLIRVHTCGVCRTDLHLLDAELPNAVFPIIPG 59 Query: 60 HEFMGVVVEAGPAVKRLKKGDRVVVPFV-IACGQCFFCDQHLYAACETTNPDRGAIING 117 HE +G +V G AV+ + GDR VP++ CGQC +C C+ + G ING Sbjct: 60 HEIVGTIVATGSAVQSFRIGDRAGVPWLGHTCGQCSYCVAGQENLCDHAS-FTGCTING 117 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.322 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 787,080 Number of extensions: 34795 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 5 Length of query: 386 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 303 Effective length of database: 726,862 Effective search space: 220239186 Effective search space used: 220239186 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)