BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0475.p (402 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095879|ref|YP_001100954.1| acridine efflux pump protein [H... 439 e-124 gi|134095558|ref|YP_001100633.1| acridine efflux pump protein [H... 293 9e-81 gi|134094274|ref|YP_001099349.1| hypothetical protein HEAR1042 [... 126 2e-30 gi|134093785|ref|YP_001098860.1| cobalt-zinc-cadmium resistance ... 71 1e-13 gi|134094811|ref|YP_001099886.1| cobalt-zinc-cadmium resistance ... 69 3e-13 >gi|134095879|ref|YP_001100954.1| acridine efflux pump protein [Herminiimonas arsenicoxydans] Length = 407 Score = 439 bits (1130), Expect = e-124, Method: Compositional matrix adjust. Identities = 228/377 (60%), Positives = 286/377 (75%), Gaps = 1/377 (0%) Query: 1 MSSVSRFTRLTMTVAVAAALVACGQKPAAQGGPMPAPEVSVVTIAPQRVAITNELPGRVE 60 MSS+ R T L + A+ CG K A P EVSVVTI + +TNELPGR+E Sbjct: 1 MSSLPRLTSLGAAFFILFAVAGCG-KSDAPAAAPPPTEVSVVTIKSSALGVTNELPGRLE 59 Query: 61 AFRTAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ 120 + R A++RARV G+V KR F+EGS+VK G LF IDPAQ+KA+ SA A +ARA+A L Q Sbjct: 60 STRIAEIRARVPGVVLKRTFREGSDVKAGATLFLIDPAQFKATYNSANAAVARAEATLLQ 119 Query: 121 TTLTAQRYKPLVEVNAVSKMDYDNAVAAQKQAEADVASGKAAVQTASLNLGYATVTSPIS 180 L +RYKPLV NA+SK +YD+ A KQAEAD+A+ KAA +TA L+L YATVTSPIS Sbjct: 120 AELKEKRYKPLVATNAISKQEYDDVTATLKQAEADLAASKAARETARLSLDYATVTSPIS 179 Query: 181 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKNVGDN 240 GRIGRALVTEGALVGQ +AT LA VQQ+DP+YVN+TQSS ++L+LQ+A+A G+LK+ G Sbjct: 180 GRIGRALVTEGALVGQNEATPLATVQQIDPIYVNMTQSSTEILQLQRALADGKLKSAGKG 239 Query: 241 KAKVTLVLENGQPYDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYVRTNIEQA 300 + KVTLV E+GQ Y QPGKLLFSD++VD S+G+++LRAEFPNP LLPG YVR +EQA Sbjct: 240 QVKVTLVTEDGQEYAQPGKLLFSDLTVDASSGAITLRAEFPNPDSQLLPGMYVRARVEQA 299 Query: 301 VDDQALLVPQQAVMRDAGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIV 360 +++ A+ VPQQA+ R G+ VMVV+AEN V RPV TG A G++W + DGLK+GD+VIV Sbjct: 300 INENAITVPQQAISRTPDGAAVMVVSAENTVVSRPVTTGAASGDSWVISDGLKDGDQVIV 359 Query: 361 EGLQKVRPGATVKPVQW 377 EGLQK++PGATVKPVQW Sbjct: 360 EGLQKIKPGATVKPVQW 376 >gi|134095558|ref|YP_001100633.1| acridine efflux pump protein [Herminiimonas arsenicoxydans] Length = 403 Score = 293 bits (750), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 183/364 (50%), Positives = 246/364 (67%), Gaps = 5/364 (1%) Query: 11 TMTVAVAA-ALVACGQKPAAQGGPMPAPEVSVVTIAPQRVAITNELPGRVEAFRTAQVRA 69 T V +AA A+ CG K AA P V+VVT+ Q V++T ELPGR AF A+VR Sbjct: 12 TFAVMLAAVAISGCGNKEAAAPAPSAP-AVTVVTLKAQEVSLTRELPGRTNAFLVAEVRP 70 Query: 70 RVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYK 129 +V GI++KR+F EG++VK GQ L+++D A Y+A+ SA+A LARAQA L L A R Sbjct: 71 QVNGIIKKRLFTEGAQVKAGQPLYQLDDAIYQANYASAKAALARAQATLKSAELNATRTA 130 Query: 130 PLVEVNAVSKMDYDNAVAAQKQAEADVASGKAAVQTASLNLGYATVTSPISGRIGRALVT 189 LV+++AVS+ D +NA AA Q++ADVA+ +AA+Q+ S+ LGYA +TSPI+GRIG++ VT Sbjct: 131 ELVKIDAVSRQDNENAEAALSQSKADVAAAQAALQSNSVTLGYAQITSPITGRIGKSSVT 190 Query: 190 EGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKNVGDNKAKVTLVLE 249 +GALV A LA VQQL+PVY+++TQSS++LL+L++ +AAG+L G+ V +VLE Sbjct: 191 QGALVTANQAAALATVQQLNPVYIDLTQSSSELLQLRKELAAGRLTQSGN--LPVQIVLE 248 Query: 250 NGQPYDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYVRTNIEQAVDDQALLVP 309 +G Y GKL F+DV+VD TGS +LR PNP +L+PG YVR I V A+LVP Sbjct: 249 DGSRYQHDGKLAFADVTVDPGTGSFALRVTVPNPDNILMPGMYVRALIGNGVRQNAILVP 308 Query: 310 QQAVMRD-AGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRP 368 QQ + RD G + MVV+ + KV RPV G+ W V DGL GDRVIV GLQK++P Sbjct: 309 QQGIARDPKGNTSAMVVDKDGKVALRPVSVSRTIGDKWLVEDGLAAGDRVIVAGLQKIKP 368 Query: 369 GATV 372 G V Sbjct: 369 GIPV 372 >gi|134094274|ref|YP_001099349.1| hypothetical protein HEAR1042 [Herminiimonas arsenicoxydans] Length = 382 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 105/337 (31%), Positives = 164/337 (48%), Gaps = 26/337 (7%) Query: 57 GRVEAFRTAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQA 116 G V A T VR +V IV+ +EG VK GQ+LF +D ++++ AQA LA Q+ Sbjct: 59 GYVTAINTVDVRPKVQNIVRTIHVREGQFVKAGQLLFTLDERSDQSNVAKAQAELAAGQS 118 Query: 117 NLTQTTLTAQRYKPLVEVNAVSKMDYD---NAVAAQKQAEADVASGKAAVQTASLNLGYA 173 +L L +R + L+ N VS+ D N VAA + + +A +++++ L Sbjct: 119 DLADAELALKRNEDLLSRNFVSQAVVDTARNKVAALRNTS---KANRAIAESSNITLSDN 175 Query: 174 TVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQ 233 + + ISGRIG V G+L Q + + QLDP+ V+ T +L + G Sbjct: 176 RIAASISGRIGIISVHPGSL-AQPSGDPMVTISQLDPIAVSFTLPERELANITATYPQGN 234 Query: 234 LKNVGDNKAKVTLVLENGQPYDQPGKLLFSDVSVDESTGSVSLRAEFPNPKRLLLPGTYV 293 A VT L GQP + GKL+F D + D +G++ ++A+F N + L PG++V Sbjct: 235 --------APVTARLP-GQP-EMTGKLIFIDNAADTQSGTIRMKAQFDNAAQKLWPGSFV 284 Query: 294 RTNIEQAVDDQALLVPQQAVMRDAGGSIVMVVNAENKVEPRPVKTGGARGNNWQVLDGLK 353 ++ A+LVP QAV+ + VV + V+ + V V+ GL Sbjct: 285 NVSLVSRNVSHAVLVPAQAVVTGPIDKFIYVVQPDATVKEQKVDVVAIEEGQAAVI-GLD 343 Query: 354 EGDRVIVEGLQKVRPGATVK--------PVQWSPAGA 382 G RV+VEG Q +RPG+ + PV+ SP + Sbjct: 344 AGARVVVEGTQNLRPGSKISEARDKSAAPVKSSPGAS 380 >gi|134093785|ref|YP_001098860.1| cobalt-zinc-cadmium resistance protein CzcB [Herminiimonas arsenicoxydans] Length = 526 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 95/321 (29%), Positives = 142/321 (44%), Gaps = 28/321 (8%) Query: 39 VSVVTIAPQRVAITNELPG--RVEAFRTAQVRARVAGIVQKRVFKEGSEVKEGQVLFKID 96 V ++T P R+ +L G R+ RT V R+AGIV G +VK+GQVL I Sbjct: 200 VKIMTAGPARIKTNLQLIGEIRLNEDRTVHVVPRLAGIVDSVRANAGDQVKKGQVLAVIS 259 Query: 97 PAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKMDYDNAVAAQKQAE--A 154 SL ++ L AQ L+ T +R K L E ++ DY A AA ++A+ A Sbjct: 260 S----QSLSDQRSELLAAQKRLSLARTTYEREKSLWEEKISAEQDYLQARAAMQEAQIMA 315 Query: 155 DVASGKAAVQTA---SLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDPV 211 A K A A S NL + SPI G I ++ G++ + DA + +V L V Sbjct: 316 QSAQQKLAALGASPSSSNLTQYEIRSPIDGVITDKNISTGSVTKE-DAN-IFVVSDLSTV 373 Query: 212 YVNVTQSSADLLKLQQAMAAGQLKNVGDNKAKVTLVLENGQPYDQPGKLLFSDVSVDEST 271 +V++T + D+ A+ GQ K V +GQ G + + V E T Sbjct: 374 WVDLTVRAEDI----GALKVGQ-------KGTVKSSAFDGQDV---GAVSYIGALVGEQT 419 Query: 272 GSVSLRAEFPNPKRLLLPGTYVRTNIEQAVDDQALLVPQQAVMRDAGGSIVMVVNAENKV 331 + R NPK L PG V N+ D + + ++ + G V+ N + Sbjct: 420 RTAKARIVLANPKGLWRPGLPVNVNLVAGEVDVPVAISVDSI-QTLGDREVVFGRYGNLL 478 Query: 332 EPRPVKTGGARGNNWQVLDGL 352 E RP++ G + G +VL GL Sbjct: 479 EARPLELGRSDGKLIEVLSGL 499 >gi|134094811|ref|YP_001099886.1| cobalt-zinc-cadmium resistance protein CzcB [Herminiimonas arsenicoxydans] Length = 524 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 88/323 (27%), Positives = 137/323 (42%), Gaps = 32/323 (9%) Query: 39 VSVVTIAPQRVAITNELPG--RVEAFRTAQVRARVAGIVQKRVFKEGSEVKEGQVLFKID 96 V ++T P R+ + +L G R+ T V R+AGIV+ G +VK+GQVL I Sbjct: 198 VQIMTAGPARIKTSLQLIGEIRLNEDSTVHVVPRLAGIVESVRANAGDQVKKGQVLAVIS 257 Query: 97 PAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKMDYDNAVAAQKQAEADV 156 SL ++ L AQ L+ T +R K L E ++ DY A A + EA + Sbjct: 258 S----QSLSDQRSELLAAQKRLSLARTTYEREKTLWEEKISAEQDYLQARAVMQ--EAQI 311 Query: 157 ASGKAAVQTASL-------NLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLD 209 + A + A+L NL + SPI+G I ++ G + + D + ++ L Sbjct: 312 MAQSAQQKLAALGASSSSSNLTQYEIRSPINGVITDKRISTGTVAKEDD--NIFVISDLS 369 Query: 210 PVYVNVTQSSADLLKLQQAMAAGQLKNVGDNKAKVTLVLENGQPYDQPGKLLFSDVSVDE 269 V+V++T + D+ A+ GQ V N V G + + V E Sbjct: 370 TVWVDLTVRAEDI----GALKTGQKGTVKSNAFDAQDV----------GTVSYIGALVGE 415 Query: 270 STGSVSLRAEFPNPKRLLLPGTYVRTNIEQAVDDQALLVPQQAVMRDAGGSIVMVVNAEN 329 T + R NPK + PG V N+ D + + +V + G V+ N Sbjct: 416 QTRTAKARIVLANPKGIWRPGLPVNVNLVAGEVDVPVTISTDSVQK-MGDRDVVFGRYGN 474 Query: 330 KVEPRPVKTGGARGNNWQVLDGL 352 +E RP+ G G +VL GL Sbjct: 475 LLEARPIDLGRTDGKLVEVLGGL 497 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.312 0.127 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 628,833 Number of extensions: 23581 Number of successful extensions: 112 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 10 Length of query: 402 Length of database: 1,000,347 Length adjustment: 84 Effective length of query: 318 Effective length of database: 723,567 Effective search space: 230094306 Effective search space used: 230094306 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)