BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0254.p (490 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095533|ref|YP_001100608.1| putative RND efflux system, out... 209 3e-55 gi|134094170|ref|YP_001099245.1| putative outer membrane protein... 119 3e-28 gi|134095560|ref|YP_001100635.1| outer membrane transport protei... 110 1e-25 gi|134095881|ref|YP_001100956.1| outer membrane transport protei... 100 2e-22 gi|134095761|ref|YP_001100836.1| putative outer membrane transpo... 88 8e-19 >gi|134095533|ref|YP_001100608.1| putative RND efflux system, outer membrane lipoprotein, NodT [Herminiimonas arsenicoxydans] Length = 499 Score = 209 bits (532), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 159/481 (33%), Positives = 235/481 (48%), Gaps = 8/481 (1%) Query: 4 MPSIFTPRPLALAVAL--LLSGCALGPSGEAPAMPQPAHYAVESTPATLGPQEAR-QKFV 60 M + P + V L L+S C +GP P Y + T Q+F Sbjct: 1 MSATINPCRYLIVVTLIQLISACTVGPDYIRQPPPTETAYTKKDPIVTTADNNGNAQRFH 60 Query: 61 LGQRAVPQWWQAYGSPQLSAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDA 120 WW + S L VD +LRNN SL A+ +L +Q L+A G +P I Sbjct: 61 TDAAISADWWNLFHSADLGEAVDRSLRNNASLQAAQASLKESQHNLQAGYG-VFFPQIGG 119 Query: 121 VGQTQRQ--RALGLPNIGPSTNVYNVFSGQIRASYTFDLFGVQRFANSALAAQVDAQSYQ 178 RQ A + P ++N+F+ SYT D+FG +R AL AQ D Q Y Sbjct: 120 SFSATRQLPSAFRFGSASP-VGIFNLFTLGAAISYTLDIFGGERRMVEALGAQADYQKYV 178 Query: 179 FDAARRTVAANVVISAINAASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELLSA 238 A T++ NVV +AI A+ AQ T++L+ + + + A G +LS Sbjct: 179 ALATYLTLSGNVVNTAIARAAYHAQYEATQQLILRQQEQLAVAENQAAHGIAPYATVLSL 238 Query: 239 RASAQSLRASLPGLKAQWQGARHALAVLMGRTPDAA-PPDLDIAALTLPAEVPMTVPSDL 297 R+ S +A+L + + A H LA L G T D+A L LP ++P+T+PS+L Sbjct: 239 RSLLASSQAALAVVAQKRDQADHLLANLQGTTASGTYTSSFDLAQLELPQDLPLTLPSEL 298 Query: 298 LRQRPDILAADAALKVAAAQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAVWGLGA 357 +RQRPD+LAA+A L VA+A ATA +FPS S+ A++ + W +G+ Sbjct: 299 VRQRPDVLAAEAQLHVASAGVGVATAALFPSFSLGANYALGNTKLGNLSDSSSKFWSVGS 358 Query: 358 SLTQPLFHGGALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQASAA 417 + P+F GG KR AA++AY A ++YRQTVL+AFQ VAD L A+ DA A++A A Sbjct: 359 GVDVPIFQGGTAWHKRRAAQDAYQQALANYRQTVLSAFQQVADVLTAIEHDAQAVKAQAE 418 Query: 418 ARTASEQSWRNAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQAMGV 477 + A+EQ+ + + + G A Q+ +++ ++A R DT LF A+G Sbjct: 419 SLGAAEQTLKLIEVNYRAGLVSYLDVLAANTQFYQSKIAYLQALGLRYQDTTALFVALGG 478 Query: 478 G 478 G Sbjct: 479 G 479 >gi|134094170|ref|YP_001099245.1| putative outer membrane protein [Herminiimonas arsenicoxydans] Length = 469 Score = 119 bits (298), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 135/487 (27%), Positives = 227/487 (46%), Gaps = 37/487 (7%) Query: 4 MPSIFTPR--PLALAVALL--LSGCALGPSGEAPAMPQPAHYAVESTPATLGPQEARQKF 59 M S F R LALAVA+L +SGCA P + PA +A E PA + Q Sbjct: 1 MTSRFNFRRTHLALAVAVLFAVSGCAHNIDASKPNVEIPATWA-EVAPAGIELQR----- 54 Query: 60 VLGQRAVPQWWQAYGSPQLSAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTID 119 WW+ +GS +LSA +D++L + L A + A++ + G SL+P + Sbjct: 55 --------DWWRGFGSNELSALIDQSLAGSPDLIIAAERVTQAEIT-ASSAGASLFPLLT 105 Query: 120 AVGQTQRQRALGLPNIGPSTNVY---NVFSGQIRASYTFDLFGVQRFANSALAAQVDAQS 176 T G GP+ + + + +Y D++G + A ++A Sbjct: 106 VSAGTDT----GQVKPGPTGSGWARSESTGVALSVNYEVDVWGRVAATTRSANASLNASR 161 Query: 177 YQFDAARRTVAANVVISAINAASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELL 236 Y + R T+ V + +LR Q+ + +A+A+ ++ R GA S ++ Sbjct: 162 YDLETVRLTLTTGVANAYFQTLALRMQLQVAQENLAIAEKLFSIVEARYRFGAASALDVS 221 Query: 237 SARASAQSLRASLPGLKAQWQGARHALAVLMGRTPDAAPPDLDIAALTLPA----EVPMT 292 R++ + RA++ L+ Q + ALA+L+GR P A L +AAL+L EV Sbjct: 222 RQRSTVLAQRATILPLQVQERQTVSALAILLGRQPQA----LQVAALSLDTLAIPEVSPG 277 Query: 293 VPSDLLRQRPDILAADAALKVAAAQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAV 352 +PS L+ +RPD+ + +A L +A A A A + P++S+ S G + S A T G A Sbjct: 278 LPSTLITRRPDVASVEAQLLAGSANVAAARAALLPTISLTGSAGNA--SAALLTLGNPAY 335 Query: 353 -WGLGASLTQPLFHGGALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADA 411 G+ AS+ Q LF G +LR + + + YR+TV A + V D+L T++ + Sbjct: 336 SIGIAASIVQTLFDGNSLRLRVETNESTRRQLAESYRRTVYTALKEVDDALGNATRNREQ 395 Query: 412 LQASAAARTASEQSWRNAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTATL 471 QA A + +E++ ++ R + GA ++ +R A + R++ T + Sbjct: 396 EQAQIAIKKEAERALALSELRYREGADDLNSLLDAQRTLFAADDKLTQLRLLRLTGTTDV 455 Query: 472 FQAMGVG 478 F+A+G G Sbjct: 456 FKALGGG 462 >gi|134095560|ref|YP_001100635.1| outer membrane transport protein involved toxic tolerance [Herminiimonas arsenicoxydans] Length = 476 Score = 110 bits (276), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 127/489 (25%), Positives = 217/489 (44%), Gaps = 38/489 (7%) Query: 13 LALAVALLLSGCALGPSGEAPAMPQ-----PAHYAVESTPATLGPQEARQKFVLGQRAVP 67 LALA+ L +SGCA+ P +P+ PA + + A G ++A Sbjct: 9 LALAITLAVSGCAM----LEPQLPKADAAIPAAWPLPPNTAAAGQNVTADTSATKEQAAA 64 Query: 68 Q--WWQAYGSPQLSAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDAVGQTQ 125 W + P+L + AL NN L A N+ A+ Q + Q E +P +D G Sbjct: 65 DIGWRDFFVDPKLEELIARALHNNRDLRVAVLNVERARAQYQIQNAER-FPALD--GSVA 121 Query: 126 RQRALGLPNIGPSTNVYNVFSGQIRASYTFDLFG-VQRFANSALAAQVDAQSYQFDAARR 184 R R+ G N+ N + +S + AS+ DLFG + + +AL Q + + +R Sbjct: 122 RNRSGG--NV---VNPGDYYSAALSASFELDLFGRIHNLSQAALE-----QYFAQEEGQR 171 Query: 185 TVAANVVISAINAASLRAQVAQTERLVALADADVEEMQRRLAL-----GAVSTDELLSAR 239 + +++ N A +++R +A A D +++ L + GAVS +L A+ Sbjct: 172 SAQLSLIAEIANTYLTFAADLESQR-IAQATFDNQQIAYDLVVKRHRAGAVSGLDLSQAQ 230 Query: 240 ASAQSLRASLPGLKAQWQGARHALAVLMGR-TPDAAPP---DLDIAALTLPAEVPMTVPS 295 + ++ RA + R+AL +L+G D+ P DL ++ + A +P +PS Sbjct: 231 TTIETARADVARYAGLVATDRNALTLLVGAPVEDSLLPQGFDLKVSGI---APLPAQLPS 287 Query: 296 DLLRQRPDILAADAALKVAAAQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAVWGL 355 +L +RPD+ + L+ A A A A FPS+S+ S G + + W Sbjct: 288 QVLLRRPDVRQTEHVLRGANANIGAARAAFFPSISLTGSVGSASNQLSGLFDSGTKFWSF 347 Query: 356 GASLTQPLFHGGALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQAS 415 ++ P+FHGGALRA A A + Y + + + F+ VAD+L + A QA Sbjct: 348 VPQISLPIFHGGALRANLGVATADRDIALARYEKAIQSGFREVADALALTSTLAQRRQAQ 407 Query: 416 AAARTASEQSWRNAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQAM 475 A A+ +++ ++ R Q G +R + A+ + A + + TL++ + Sbjct: 408 QALLDAASRAYDLSKARYQYGRDSFLILLDAQRTFYTAQQSLVAAQLAEQGNRVTLYKVL 467 Query: 476 GVGLEAQAQ 484 G G E Q Sbjct: 468 GGGWEEGTQ 476 >gi|134095881|ref|YP_001100956.1| outer membrane transport protein involved in copper (silver) tolerance [Herminiimonas arsenicoxydans] Length = 474 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 119/472 (25%), Positives = 206/472 (43%), Gaps = 20/472 (4%) Query: 13 LALAVALLLSGCALGPSGEAPAMPQPAHYAVESTPATLGPQEARQKFVLGQRAVPQWWQA 72 L + A +LS C++ P P P V + +Q QRA W+ Sbjct: 7 LTVLAASVLSACSMAPKYTRPDAP------VAADYPAASAAADKQ-----QRAHETGWRN 55 Query: 73 YGSPQ-LSAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDAVGQTQRQR--- 128 + Q L A + AL NN L A + A+ Q Q + L P ++ R R Sbjct: 56 FFPDQRLQALIGAALANNRDLRVAALRIEEARAQYNIQSAD-LLPNLNGTFTGSRARTPA 114 Query: 129 ALGLPNIGPSTNVYNVFSGQIRASYTFDLFG-VQRFANSALAAQVDAQSYQFDAARRTVA 187 ++ I ++ Y V G AS+ D FG V ++ALA+ + + Q AA ++ Sbjct: 115 SISPTGIDSVSSAYQV--GLSLASFELDFFGRVHSLNDAALASYLATEEAQ-RAAHISLV 171 Query: 188 ANVVISAINAASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELLSARASAQSLRA 247 A V + + + Q+A + + + ++R +GA S +L A QS R Sbjct: 172 AEVAKAYLAERAFAEQLALAQSTLKSRGTAYDLAKQRFEVGASSALDLRQNEALLQSARV 231 Query: 248 SLPGLKAQWQGARHALAVLMGRTPDAAPPDLDIAALTLPAEVPMTVPSDLLRQRPDILAA 307 + L Q A +AL +L+G+ P ++A + ++P +PSDLL QRPDI +A Sbjct: 232 AQVTLARQRAQALNALTLLVGQPLTGFPEAQPLSAQNIVTDIPAGLPSDLLTQRPDIRSA 291 Query: 308 DAALKVAAAQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAVWGLGASLTQPLFHGG 367 + +L A A A A FP +S+ A G + + + W L P+F G Sbjct: 292 EQSLLAANANIGAARAAFFPRISLTAGIGTASSTLSNLFDSGTRAWTFAPQLVLPIFDAG 351 Query: 368 ALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQASAAARTASEQSWR 427 A ++ A ++Y +++ +AF+ V+D+LVA + + A + A + + Sbjct: 352 RNSANLTLSEVRKNIAIANYEKSIQSAFREVSDALVARDLLEEQIDAQRRVQEAQAERLK 411 Query: 428 NAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQAMGVGL 479 A +R Q G + +R+ +A ++A R+++ L++++G GL Sbjct: 412 LADQRFQNGIASSLDVLDAQRELFSAEQDLVQARLLRLTNAIDLYRSLGGGL 463 >gi|134095761|ref|YP_001100836.1| putative outer membrane transport protein involved in copper (silver) tolerance (cusC) [Herminiimonas arsenicoxydans] Length = 502 Score = 88.2 bits (217), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 117/424 (27%), Positives = 186/424 (43%), Gaps = 28/424 (6%) Query: 69 WWQAYGSPQLSAWVDEALRNNHSLAQAERNLAGAQLQLRAQVGESLWPTIDAVGQTQRQR 128 W + +L + +AL NN L A + A+ Q E +P I A ++R Sbjct: 66 WRDYFTDARLQDLIAQALENNRDLRAAVLRVEEARAAYGIQRAEQ-FPAIAAQAGSERAH 124 Query: 129 A---LGLPNIGPSTNVYNVFSGQIRASYTFDLFG-VQRFANSALAAQVDAQSYQFDAARR 184 L L ++ Y+V G AS+ D +G V+ ++AL + + D ARR Sbjct: 125 VPADLSLTGKPAMSSQYHVSLGL--ASWEIDFWGRVRSLRDAALENYLAS-----DEARR 177 Query: 185 TVAANVVISAINA----ASLRAQVAQTERLVALADADVEEMQRRLALGAVSTDELLSARA 240 V ++V N+ L ++A +A + + RR+ +G+ S L Sbjct: 178 AVTISLVAQVANSYLALCELDERIALARLTIASREESLRIFTRRVEVGSTSRLNL----T 233 Query: 241 SAQSLRASLPGLKAQWQGAR----HALAVLMGRTPD--AAPPDLDIAALTLPAEVPMTVP 294 Q+L + L AQ + AR +AL +L+G T + A LD + LP + + P Sbjct: 234 QVQTLLSQAQALGAQLEQARALQANALILLVGVTLELPATSSRLDERQMLLPLQPGL--P 291 Query: 295 SDLLRQRPDILAADAALKVAAAQQAEATAEMFPSLSINASFGQSGFSWAKATSGVGAVWG 354 SDLL +RPDI+AA+ LK A AQ A A FP +++ SFG + + W Sbjct: 292 SDLLIRRPDIVAAEHQLKAAHAQIGAARAAFFPRIALTGSFGTASAELDGLFAAGSRAWT 351 Query: 355 LGASLTQPLFHGGALRAKRDAAKEAYLAAQSHYRQTVLNAFQNVADSLVALTQDADALQA 414 S++ PLF G A+ A ++Y +T+ AF++VAD+L A A L Sbjct: 352 FAPSISLPLFDAGRRSNNLSLAEARRDLAVANYEKTIQAAFRDVADALAARQWLAQQLLI 411 Query: 415 SAAARTASEQSWRNAQRRAQLGAYPASATRAGERQYLNARLGEIRATASRMSDTATLFQA 474 + AA T + R +Q R GA +R L A ++ + +S +L+ A Sbjct: 412 AQAALTTQTERARLSQLRYDNGAAAYLDVLDAQRDLLVAEQQLVQIRRALLSSQVSLYAA 471 Query: 475 MGVG 478 +G G Sbjct: 472 LGGG 475 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.316 0.125 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 703,562 Number of extensions: 23857 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 7 Length of query: 490 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 405 Effective length of database: 720,272 Effective search space: 291710160 Effective search space used: 291710160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)