BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0250.p (383 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094046|ref|YP_001099121.1| poly[D(-)-3-hydroxybutyrate] de... 88 9e-19 gi|134095518|ref|YP_001100593.1| Poly(3-hydroxyalkanoate) polyme... 56 3e-09 gi|134095122|ref|YP_001100197.1| Poly-beta-hydroxybutyrate polym... 55 8e-09 >gi|134094046|ref|YP_001099121.1| poly[D(-)-3-hydroxybutyrate] depolymerase [Herminiimonas arsenicoxydans] Length = 411 Score = 87.8 bits (216), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 80/323 (24%), Positives = 140/323 (43%), Gaps = 32/323 (9%) Query: 76 RTLTLREYGKPAAVPTLVVAPYAGHSSVIADYYHGQSLVETLMAHGVSH-VCLTDWHSAS 134 + L+ +E+ L+VAP +GH S + L +T+ + H V +TDW A Sbjct: 94 KKLSAKEFAALKQPVVLLVAPLSGHHSTL--------LRDTVRSLLQDHDVYITDWTDAR 145 Query: 135 E---DMKDLEIDNYLADLCVVIQELGGRVNLVGLCQG-----GWISAMIAARFPPMVQRL 186 + +Y+ + I+ LG ++++ +CQ IS M + R P + + + Sbjct: 146 MVPLAEGPFHLHDYVYYIQDFIRRLGPDLHVISVCQPTVPVLAAISLMASERDPRLPKTM 205 Query: 187 VLAGSPIDAGAGDGPLKQMVERTPIRFYEDLVRT---------GGGLMRGSFMLQGWKNM 237 + G PIDA + + P ++E+ V G + G G+ M Sbjct: 206 TMMGGPIDARKSPTQVNDLAVEKPHAWFENQVIYSVPPSYPGFGRKVYPGFLQHAGFIAM 265 Query: 238 HPDEHYFTEHLDLFQHID--DPVYLAKRETFSAWYETTVDLPGRWYLQAIRNIFKDNLLA 295 +P H H D + H+ D + F Y +D+P +YL+ I+ +F+++ L Sbjct: 266 NPGRHA-QSHWDFYMHLREGDNESAEEHRKFYDEYNAVLDMPAEYYLETIKTVFQEHRLP 324 Query: 296 RGKYVALGKQLDLKDIH-CPLYLLAGERDDITTAEQVMNAATLMGT-PATHITQRVVPG- 352 G + GK + +DI L+ + GE+DDI+ A Q A L P + Q P Sbjct: 325 LGTWEIEGKLVRPQDIKSVALFTIEGEKDDISGAGQTHAAQDLCSALPQSDKQQFTAPQC 384 Query: 353 GHIGLFMGSRTLAETWPQIAQWL 375 GH G+F G + P+I +++ Sbjct: 385 GHYGIFSGRKWREIIAPKIGEFI 407 >gi|134095518|ref|YP_001100593.1| Poly(3-hydroxyalkanoate) polymerase, PHA synthase, PhbC-like [Herminiimonas arsenicoxydans] Length = 625 Score = 55.8 bits (133), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 69/285 (24%), Positives = 118/285 (41%), Gaps = 37/285 (12%) Query: 88 AVPTLVVAPYAGHSSVIADYYHGQSLVETLMAHGVSHVCLTDWHSASEDMKDLEIDNY-- 145 AVP L V P I D SLV +++ G + V + W + + ++L +++Y Sbjct: 281 AVPLLFV-PAWIMKYYILDLSPQNSLVRYMVSQGYT-VFMISWKNPLAEDRNLSMEDYRK 338 Query: 146 LADLCVV--IQEL--GGRVNLVGLCQGGWI----SAMIAARFPPMVQRLVLAGSPIDAGA 197 L + + I EL ++N G C GG + SA +A + + L + +D Sbjct: 339 LGVMAAIDAITELTHAPQLNAAGYCLGGTLLSIASASMACHGDERIASMSLFAAQVDFEE 398 Query: 198 GDGPLKQMVERTPIRFYEDLVRTGGGL----MRGSFMLQGWKNMHPDEHYFTEHLDLFQH 253 G L ++ + I F E ++ G L M G+F L + ++ ++ L+ Sbjct: 399 P-GELSLFIDESQISFLEAIMWDQGYLDTKQMAGAFQL-----LRSNDLIWSRRLN---- 448 Query: 254 IDDPVYL----AKRETFSAWYETTVDLPGRWYLQAIRNIFKDNLLARGKYVALGKQLDLK 309 YL K+ W +P R + + +R++F N LA G+Y GK + L Sbjct: 449 ----QYLLGLSEKKSDLMTWNADATRMPYRMHSEYLRHLFLHNDLAEGRYFTEGKSIHLT 504 Query: 310 DIHCPLYLLAGERDDITTAEQVMNAATLMGTPATHITQRVVPGGH 354 DI P++ + D + V L T +T + GGH Sbjct: 505 DISIPIFAVGTLTDHVAPWRSVFKIHRLAD---TEVTFALTSGGH 546 >gi|134095122|ref|YP_001100197.1| Poly-beta-hydroxybutyrate polymerase [Herminiimonas arsenicoxydans] Length = 537 Score = 54.7 bits (130), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 64/266 (24%), Positives = 111/266 (41%), Gaps = 30/266 (11%) Query: 73 LKLRTLTLREYGKPAAVPTLVVAPYAGHSSVIADYYHGQSLVETLMAHGVSHVCLTDWHS 132 L+ + LT Y +P L++ P + I D S V + G + V L W + Sbjct: 174 LQYKALTPTVYERP-----LLLVPPCINKYYILDLQPHNSFVRYAVEQGHT-VFLISWRN 227 Query: 133 ASEDMKDLEIDNYLAD---------LCVVIQELGGRVNLVGLCQGGWISAMIAA----RF 179 A + D+Y+ + +QE ++N++G C GG + A A R Sbjct: 228 ADASIAHATWDDYVESGVLQAISVAQAISVQE---KINVLGFCVGGTMLANALALACARG 284 Query: 180 PPMVQRLVLAGSPIDAGAGDGPLKQMVERTPIRFYEDLVRTGGGLMRGSFMLQGWKNMHP 239 L L + +D + G L ++ I+ E+L+ GGLM+G + ++ Sbjct: 285 EKPAASLTLLTTFLDF-SDTGILDVYIDEAKIKETEELI-GHGGLMKGLDFNAAFASLRS 342 Query: 240 DEHYFTEHLDLFQHIDDPVYLAKRETFSAWYETTVDLPGRWYLQAIRNIF-KDNLLARGK 298 +E + + ++PV W + +LPG Y +RN + ++NL GK Sbjct: 343 NELIWNYVTSNYLKGENPVPF----DMLYWNADSTNLPGPMYCWYVRNTYLENNLKQLGK 398 Query: 299 YVALGKQLDLKDIHCPLYLLAGERDD 324 + GK++D I P ++ G RDD Sbjct: 399 FKLAGKKIDFGKIDAPSFIY-GSRDD 423 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.322 0.136 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 758,649 Number of extensions: 31730 Number of successful extensions: 81 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 3 Length of query: 383 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 300 Effective length of database: 726,862 Effective search space: 218058600 Effective search space used: 218058600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)