BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0120.p (701 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096324|ref|YP_001101399.1| elongation factor G [Herminiimo... 1274 0.0 gi|134094178|ref|YP_001099253.1| peptide chain release factor 3 ... 165 8e-42 gi|134095607|ref|YP_001100682.1| GTP-binding elongation factor f... 97 3e-21 gi|134095256|ref|YP_001100331.1| GTP-binding protein LepA [Hermi... 92 1e-19 gi|134096335|ref|YP_001101410.1| elongation factor Tu [Herminiim... 60 3e-10 >gi|134096324|ref|YP_001101399.1| elongation factor G [Herminiimonas arsenicoxydans] Length = 702 Score = 1275 bits (3298), Expect = 0.0, Method: Compositional matrix adjust. Identities = 613/700 (87%), Positives = 651/700 (93%) Query: 1 MARKTPIERYRNIGISAHIDAGKTTTTERILYYTGVNHKIGEVHDGAATMDWMEQEQERG 60 MARKTPIERYRNIGISAHIDAGKTTTTER+L+YTGVNHK+GE H+G+AT DWMEQEQERG Sbjct: 1 MARKTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKLGETHEGSATTDWMEQEQERG 60 Query: 61 ITITSAATTCFWKGMANNFPEHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ 120 ITITSAA TCFWKGMANN P HHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ Sbjct: 61 ITITSAAVTCFWKGMANNLPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ 120 Query: 121 PQSETVWRQANKYGVPRLAFVNKMDRTGANFFKVYEQMRSRLKANPIPIQVPIGAEDNFE 180 PQSETVWRQANKY VPRLAFVNKMDRTGANFFKVYEQMR+RLKANP+PIQVPIGAED F Sbjct: 121 PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYEQMRARLKANPVPIQVPIGAEDQFF 180 Query: 181 GVIDLVKMRAIYWDDASQGMKFDYRDIPENLKATAQEWREKMVETAAESSEDLMNKYLEE 240 GV+DL+KM++I WD+ASQGMKF+Y +IP NL A AQ+WRE MVE AAE+SE+LMNKYLEE Sbjct: 181 GVVDLIKMKSIIWDEASQGMKFEYGEIPANLLAEAQKWRENMVEAAAEASEELMNKYLEE 240 Query: 241 GDLSEEDVKKALRARTLAGEIVPMMCGTAFKNKGVQAMLDAVIEYLPAPTDIPPVKGIAE 300 GDLSE ++K ALR RT+A EIVPMMCGTAFKNKGVQAMLDAVI+YLP+P DIPPV G+ E Sbjct: 241 GDLSEAEIKAALRTRTIANEIVPMMCGTAFKNKGVQAMLDAVIDYLPSPIDIPPVPGLNE 300 Query: 301 DETETVRKAADDEKFSALAFKIMTDPFVGQLIFFRVYSGVVNSGDTVYNPVKNKKERLGR 360 + VRKA D EKFSALAFKI TDPFVGQL F R YSG +NSGDTV N VK KKER+GR Sbjct: 301 SDEPIVRKAEDGEKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTVLNSVKEKKERIGR 360 Query: 361 ILQMHANQREEIKEVRAGDIAAAVGLKEATTGDTLCDPSAIVTLERMVFPEPVISQAVEP 420 I+QM ANQREEIKE+RAGDIAA VGLK+ TTGDTLCD A V LERMVFPEPVISQAVEP Sbjct: 361 IVQMQANQREEIKEMRAGDIAAVVGLKDTTTGDTLCDDKAFVVLERMVFPEPVISQAVEP 420 Query: 421 KTKADQEKMGLALNRLAQEDPSFRVKTDEESGQTIISGMGELHLDIIVDRMRREFGVEAT 480 KTKADQEKMGLALNRLA EDPSFRV+TDEESGQTII+GMGELHLDIIVDRM+REF VEAT Sbjct: 421 KTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRMKREFNVEAT 480 Query: 481 VGKPQVAYRETIRKTCDEIEGKFVKQSGGRGQYGHVVLKIEPQEPGAGFEFVDAIKGGVV 540 VGKPQVAYRETIRKTC+EIEGKFVKQSGGRGQYGHVVLKIEPQEPG GFEFVDAIKGG V Sbjct: 481 VGKPQVAYRETIRKTCEEIEGKFVKQSGGRGQYGHVVLKIEPQEPGKGFEFVDAIKGGTV 540 Query: 541 PREFIPAVEKGIRETLNTGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAGSMAFKDGC 600 PRE+IPAVEKG+RETL +GVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMA SMAFKDGC Sbjct: 541 PREYIPAVEKGVRETLTSGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAASMAFKDGC 600 Query: 601 RKASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGMVQGMDEIAGGGGKIIKAEVPLSEM 660 RKASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGMVQGMDEIAGGGGKIIKAEVPLSEM Sbjct: 601 RKASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGMVQGMDEIAGGGGKIIKAEVPLSEM 660 Query: 661 FGYSTSLRSATQGRATYTMEFKHYSEAPKNVIDAIVTSKA 700 FGY+TSLRSATQGRATY+MEFKHYSEAPKNVIDAIVTSKA Sbjct: 661 FGYATSLRSATQGRATYSMEFKHYSEAPKNVIDAIVTSKA 700 >gi|134094178|ref|YP_001099253.1| peptide chain release factor 3 [Herminiimonas arsenicoxydans] Length = 564 Score = 165 bits (417), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 138/514 (26%), Positives = 234/514 (45%), Gaps = 44/514 (8%) Query: 7 IERYRNIGISAHIDAGKTTTTERILYYTGVNHKIGEVHDGA----ATMDWMEQEQERGIT 62 + R R GI +H DAGKTT TE++L ++G G V AT DWM+ E++RGI+ Sbjct: 40 VARRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKGRKSARHATSDWMDIEKQRGIS 99 Query: 63 ITSAATTCFWKGMANNFPEHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQ 122 + S+ M + +H +N++DTPGH DF+ + R + +D A MV A GV+ Q Sbjct: 100 VASSV-------MQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVETQ 152 Query: 123 SETVWRQANKYGVPRLAFVNKMDRTGANFFKVYEQMRSRLKANPIPIQVPIGAEDNFEGV 182 + + P + F+NK+DR + ++ +++ S LK P+ PIG NF GV Sbjct: 153 TIKLLNVCRMRNTPIITFMNKLDRETRDPLELLDELESVLKIECAPVTWPIGMGKNFRGV 212 Query: 183 IDLVKMRAIYWDDASQGMKFDYRDIPENLKATAQEWREKMVETAAESSEDL-MNKYLEEG 241 L++ + + S+ + E +K ++ E + E L M L G Sbjct: 213 YHLLRDEIMLFAPGSEKADQTF----EVVKGIDN---PRLAEMFPQEIEQLKMEVELVHG 265 Query: 242 DLSEEDVKKALRARTLAGEIVPMMCGTAFKNKGVQAMLDAVIEYLPAPTDIPPVKGIAED 301 D+ L G P+ G+A N GV+ +L+A++++ P + Sbjct: 266 ASHPFDLDAFLN-----GTRTPVFFGSAINNFGVREILNALLDWAQPP----------RE 310 Query: 302 ETETVRKAADDE-KFSALAFKIMT--DP-FVGQLIFFRVYSGVVNSGDTVYNPVKNKKER 357 TVR E FS FKI DP ++ F RV SG G V + N++ + Sbjct: 311 RDATVRSVKPIELPFSGFVFKIQANMDPAHRDRIAFLRVCSGRFERGMKVKHLRLNREIK 370 Query: 358 LGRILQMHANQREEIKEVRAGDIAAAVGLKEATTGDTLCDPSAIVTLERMVFPEPVISQA 417 + ++ A+ RE+++E AGDI GD+ + ++ + + P +A Sbjct: 371 VSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQIGDSFSE-GELLQFTGIPYFAPDFFRA 429 Query: 418 VEPKTKADQEKMGLALNRLAQEDPSFRVKTDEESGQTIISGMGELHLDIIVDRMRREFGV 477 V + +++ L +L +E + +V S I+ G+G L +++ R+ E+GV Sbjct: 430 VRIRNPLKIKQLHKGLQQLGEEG-AIQVFKPVNSSDLILGGVGVLQFEVVASRLLNEYGV 488 Query: 478 EATVGKPQVAYRETIRKTCDE--IEGKFVKQSGG 509 +A ++ I TCD+ + F + S G Sbjct: 489 DAVFEGTSISSARWI--TCDDKKMLADFERSSAG 520 >gi|134095607|ref|YP_001100682.1| GTP-binding elongation factor family protein [Herminiimonas arsenicoxydans] Length = 612 Score = 97.1 bits (240), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 56/139 (40%), Positives = 78/139 (56%), Gaps = 9/139 (6%) Query: 11 RNIGISAHIDAGKTTTTERILYYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTC 70 RNI I AH+D GKTT +++L +G ++ A MD + E+ERGITI S Sbjct: 9 RNIAIIAHVDHGKTTLVDQLLRQSGTFRDNQQID--ARVMDSGDIEKERGITILSKNCAV 66 Query: 71 FWKGMANNFPEHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQA 130 +KG HINI+DTPGH DF EVER + ++D ++ A G PQ+ V R+A Sbjct: 67 EYKGT-------HINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKA 119 Query: 131 NKYGVPRLAFVNKMDRTGA 149 G+ + +NK+DR GA Sbjct: 120 LALGLKPIVVLNKIDRPGA 138 Score = 63.2 bits (152), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 62/229 (27%), Positives = 105/229 (45%), Gaps = 33/229 (14%) Query: 275 VQAMLDAVIEYLPAPTDIPPVKGIAEDETETVRKAADDEKFSALAFKIMTDPFVGQLIFF 334 ++ + DA++E++PA D P A + ++L + +VG++ Sbjct: 186 MEPLFDAILEHVPARQDDP--------------DAPLQLQITSLEYS----SYVGKIGVG 227 Query: 335 RVYSGVVNS-GDTVY-NPVKNK--KERLGRILQMHANQREEIKEVRAGDIAAAVGLKEAT 390 R+ +G V + D V+ N +K K R+ ++L R + E AGDI G++E Sbjct: 228 RILAGRVKALQDVVWMNGPDDKPTKARINQVLTFKGLDRVLVDEALAGDIVLINGIEEIG 287 Query: 391 TGDTLCDPSAIVTLERMVFPEPVISQ--AVEPKTKADQE-------KMGLALNRLAQEDP 441 G T+C P L + EP ++ V A +E ++ L R + + Sbjct: 288 IGTTICAPDHPNGLPMLKVDEPTLTMNFMVNNSPLAGREGKFVTTRQIRDRLERELKANM 347 Query: 442 SFRVKTDEESGQTI-ISGMGELHLDIIVDRMRREFGVEATVGKPQVAYR 489 + RV E T +SG GELHL I+++ MRRE G E V +P+V ++ Sbjct: 348 ALRVVQSENDDSTYEVSGRGELHLTILIENMRRE-GFELAVSRPRVVFK 395 >gi|134095256|ref|YP_001100331.1| GTP-binding protein LepA [Herminiimonas arsenicoxydans] Length = 597 Score = 91.7 bits (226), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 53/140 (37%), Positives = 78/140 (55%), Gaps = 5/140 (3%) Query: 11 RNIGISAHIDAGKTTTTERILYYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTC 70 RN I AHID GK+T +RI+ G + + A +D M+ E+ERGITI + Sbjct: 5 RNFSIIAHIDHGKSTLADRIIQLCG---GLSDREMEAQVLDSMDLERERGITIKAQTAAL 61 Query: 71 FWKGMANNFPEHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQA 130 +K A + +++N+IDTPGHVDF+ EV RS+ +GA +V A GV+ Q+ A Sbjct: 62 TYK--ARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYMA 119 Query: 131 NKYGVPRLAFVNKMDRTGAN 150 GV + +NK+D A+ Sbjct: 120 LDLGVEVVPVLNKIDLPNAD 139 >gi|134096335|ref|YP_001101410.1| elongation factor Tu [Herminiimonas arsenicoxydans] Length = 396 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 43/135 (31%), Positives = 62/135 (45%), Gaps = 10/135 (7%) Query: 12 NIGISAHIDAGKTTTTERILYYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTCF 71 N+G H+D GKTT T I T ++ K G G +D +E+ RGITI ++ Sbjct: 14 NVGTIGHVDHGKTTLTAAIA--TVLSKKFGGEAKGYDQIDNAPEEKARGITINTSH---- 67 Query: 72 WKGMANNFPEHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQAN 131 + H +D PGH D+ + +DGA +V A G PQ+ A Sbjct: 68 ---VEYETTTRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLAR 124 Query: 132 KYGVPR-LAFVNKMD 145 + GVP + F+NK D Sbjct: 125 QVGVPYIIVFLNKCD 139 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.317 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,298,403 Number of extensions: 54346 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 7 Length of query: 701 Length of database: 1,000,347 Length adjustment: 88 Effective length of query: 613 Effective length of database: 710,387 Effective search space: 435467231 Effective search space used: 435467231 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)