BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0038.p (568 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 179 3e-46 gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Hermi... 162 5e-41 gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 137 2e-33 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 179 bits (455), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 166/571 (29%), Positives = 263/571 (46%), Gaps = 46/571 (8%) Query: 36 DQQERTPYAWHIPEFFNIAIAACDRW--ADGSGRTAILCEENDGSLTTWSFDQLKTLSDQ 93 D+ Y W N++ DR + + AI+ E +DG++T ++ +L + Sbjct: 66 DESNAPFYKWFEDGELNVSWNCLDRHLHNGNADKVAIIFEADDGNVTRITYKELHERVCK 125 Query: 94 FANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSE 153 FAN L I +GDR+ IY+ I+ + A ++GA +F F +++ R+ ++ Sbjct: 126 FANGLKSLSINKGDRVIIYMPMSIDGIAAMQACARIGATHSVVFGGFSAKSLHERIIDAG 185 Query: 154 AVALVT---DASGMDKLHLAGEL-PALKVVFCVEPSDIVLPQTT----DFWDR------- 198 AVA++T G L L + AL + C ++V+ + T DF +R Sbjct: 186 AVAVLTADYQVRGGKNLPLKAIVDEALAMGGCDTIRNVVVYKRTGGAVDFVERRDIWLHE 245 Query: 199 ---LRAASPVLEPVMTRADDAAMIIYTSGTTGKAKGALHAHR-VLLGHLPGVEVSHDSFP 254 R + EPV A+ I+YTSG+TGK KG H+ LL + ++ D Sbjct: 246 LVEQRTSECAPEPV--GAEHPLFILYTSGSTGKPKGVQHSSAGYLLWAMLTMKWVFDI-- 301 Query: 255 QPGDRFWTPADWAWIGGLLDVLLPALYHGVA-VLARRLEKF-DAETVFGLLARHQIRNVF 312 +P D +W AD W+ G V L G ++ + + +A + ++ +H++ + Sbjct: 302 KPNDVYWCTADIGWVTGHTYVTYGPLAVGATQIVFEGVPMYPNAGRFWEMVQKHKVSIFY 361 Query: 313 FPPTALKML--RGTPDPR---SRWPFS-LRSVASGGETLGDDLIAWGREALG---VTINE 363 PTA++ L DP S++ S LR + S GE + + W + +G I + Sbjct: 362 TAPTAIRSLIKAAEADPSTHPSKYDLSSLRLLGSVGEPINPEAWMWYYKNIGGERCPIVD 421 Query: 364 FYGQTECN-LVVSSSSQLYPSASGSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRAPDP 422 + QTE V+S P GS +PG +VD+ GH LP G+ G + I+ P P Sbjct: 422 TFWQTETGGHVISPLPGATPMVPGSCTLPLPGIIAAVVDDGGHDLPNGKGGLLVIKRPWP 481 Query: 423 VMFLRYWRNEDATREKF-----AGDFLLTGDLG-NMDEAGYIRYLGRNDDVITSAGYRIG 476 M W + + + + G L GD +E GY GR DDV+ +G+R+G Sbjct: 482 AMIRNIWNDPERFEKSYFPHELGGRTYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMG 541 Query: 477 PAPIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHAPG---AALVAELQQHVRA 533 A IE L+ +P V AAVVG D E + AFVVLK G + EL+ V Sbjct: 542 TAEIESALVANPMVAEAAVVGKPDETTGESICAFVVLKRVRPEGDEAKQIAKELRDWVAK 601 Query: 534 QLAAHEYPRMIAFVDALPTTATGKIMRKTLK 564 ++ P+ I F D LP T +GKIMR+ L+ Sbjct: 602 EIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 632 >gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 634 Score = 162 bits (409), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 159/572 (27%), Positives = 262/572 (45%), Gaps = 65/572 (11%) Query: 45 WHIPEFFNIAIAACDRW----ADGSGRTAILCEENDGSLTTWSFDQLKTLSDQFANALAE 100 W + ++ A DR AD AI E + S ++F QL ++ A + Sbjct: 50 WFVGGETSLCYNAVDRHLATRADQPALIAISTETD--SEKVYTFAQLHAEVNRCAAMMQS 107 Query: 101 AGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSEAVALVTD 160 G+ +GDR+ IY+ E LA ++GA+ +F F ++A R+D++ V +++ Sbjct: 108 LGVSQGDRVLIYMPMVAEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPVLILSA 167 Query: 161 ASGMDKLH-------------LAGELPA--LKVVFCVEPSDIVLPQTTDFW----DRLRA 201 +G H LA P L V + P V + D+ + A Sbjct: 168 DAGSRGCHVMPYKSLLDEAIVLAEHKPTHVLMVNRGLAPMQTVAGRDIDYATLREKHMDA 227 Query: 202 ASPVLEPVMTRADDAAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSF--PQPGDR 259 A PV V ++ + I+YTSGTTGK KG + R + G+ + S D + G+ Sbjct: 228 AVPV---VWLESNAPSYILYTSGTTGKPKGVV---RDVGGYAVALTTSMDLIFCGKAGET 281 Query: 260 FWTPADWAWIGGLLDVLLPALYHGVA-VLARRLE-KFDAETVFGLLARHQIRNVFFPPTA 317 F+ +D W+ G ++ L G+A VL L + DA + ++ ++++ + PTA Sbjct: 282 FFCTSDIGWVVGHSYIIYGPLLAGMATVLYEGLPIRPDASIWWSIVEKYKVTRMSSAPTA 341 Query: 318 LKMLRGTPDP--RSRWPFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVS 375 +++L+ P + SL+++ GE L W L V + + Y QTE + Sbjct: 342 IRVLKKQPLELLKKHDLSSLKALYLAGEPLDQTTSEWISGVLNVPVIDNYWQTETGWPII 401 Query: 376 SSSQLY---PSASGSMGRAVPGHEVQIVDE-EGHLLPRGQVGHIAIRAPDPVMFLR---- 427 S ++ P+ SGS G + G++ +I++E G + G + I P P L+ Sbjct: 402 SIAKGVGDKPTRSGSPGLPLYGYKTKIINEISGEECAANEKGVVIIEGPLPPGCLQTLYG 461 Query: 428 --------YWRNEDATREKFAGDFLLTGDLGNMDEAGYIRYLGRNDDVITSAGYRIGPAP 479 YWRN RE ++ T D G D GY LGR DDVI AG+R+G Sbjct: 462 DDQRFVSTYWRN--FKREAYS-----TFDWGIRDADGYYFILGRTDDVINVAGHRLGTRE 514 Query: 480 IEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHAPGA-----ALVAELQQHVRAQ 534 IEE + H V AVVGV++ ++ + AFV+LK + L AE+ + V + Sbjct: 515 IEESISSHGNVSEVAVVGVENPIKGQCAVAFVILKNAALAASPEEAKKLEAEIMKVVDKR 574 Query: 535 LAAHEYPRMIAFVDALPTTATGKIMRKTLKEM 566 L + P + FV ALP T +GK++R++++ + Sbjct: 575 LGSIGRPSHVYFVSALPKTRSGKLLRRSIQAI 606 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 137 bits (344), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 154/546 (28%), Positives = 244/546 (44%), Gaps = 65/546 (11%) Query: 60 RWADGSGRTAILCEENDGSLTTWSFDQLKTLSDQFANALAEAGIGRGDRIGIYLSQRIET 119 ++AD R A +C D LT + ++ TLS + L G+ +G R+ + + ++ Sbjct: 35 KYAD---RHAYVCM--DKFLT---YAEVDTLSQKLGAWLQGTGLQKGARVALMMPNVLQY 86 Query: 120 VITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSEAVALV---TDASGMDKL--------- 167 + A + G V V + L+ P+ + ++L +S A A++ A+ ++K+ Sbjct: 87 PVALAAVLRAGYVVVNVNPLYKPRELQHQLVDSGAEAIIILENFATTLEKIIVSTQIKHV 146 Query: 168 ------HLAGELPALKVVFCVEPSD-----IVLPQTTDFWDRL-RAASPVLEPVMTRADD 215 + G L L V F V LPQ F D L +AA L+ + D Sbjct: 147 VVATMGDMLGGLKGLLVNFVVRHVKKMVPAFSLPQAVSFKDVLAQAAHMHLQRPLLGPQD 206 Query: 216 AAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQPGDRFWTPADWAWIGGLLDV 275 A + YT GTTG AKGA+ HR ++ ++ + ++++ P R P L V Sbjct: 207 IAFLQYTGGTTGTAKGAMLTHRNIVANI----LQNEAWIWPVLRKEPPDK-----TLNFV 257 Query: 276 LLPALYH-----GVAVLARRLEKF--------DAETVFGLLARHQIRNVFFPPTALKMLR 322 LYH +L RL D ++ L +++I + T L Sbjct: 258 CALPLYHIFALTACCMLGTRLGVMNILIPNPRDIPSLVKELMKYKINLLPAVNTLYNGLL 317 Query: 323 GTPDPRSRWPFSLRSVASGGETLGDDLIA--WGREALGVTINEFYGQTECNLVVSSSSQL 380 PD ++ FS + +GG ++ W R G +I E YG +E + V +S+ Sbjct: 318 NNPD-FAKVDFSELKLCNGGGMAVQQVVNDRW-RVVTGCSIIEGYGLSETSPVATSNPVE 375 Query: 381 YPSASGSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRAPDPVMFLRYWRNEDATREKFA 440 SG++G VP EV I+D + + G G IAIR P + YW N T + Sbjct: 376 TTEFSGTIGLPVPSTEVMILDSNDNEVALGLSGEIAIRGPQ--VMAGYWNNPAETAQAMT 433 Query: 441 GD-FLLTGDLGNMDEAGYIRYLGRNDDVITSAGYRIGPAPIEECLMRHPAVRIAAVVGVK 499 D F TGD+G MD GY R + R D+I +G+ + P+ IE + HP V A +GV Sbjct: 434 ADGFFKTGDIGVMDARGYTRIVDRKKDMILVSGFNVYPSEIENVVAGHPGVLECACIGVP 493 Query: 500 DALRTEVVKAFVVLKEGHAPGAALVAELQQHVRAQLAAHEYPRMIAFVDALPTTATGKIM 559 D E VK FVV ++ AL+A + Q ++ P+MI F LP + GKI+ Sbjct: 494 DEHTGEAVKLFVVRRDPALTAEALMA----FCKEQFTGYKKPKMIEFRTELPKSNVGKIL 549 Query: 560 RKTLKE 565 R+ L++ Sbjct: 550 RRELRD 555 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.321 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,156,355 Number of extensions: 51036 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 4 Length of query: 568 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 481 Effective length of database: 713,682 Effective search space: 343281042 Effective search space used: 343281042 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)