BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4683.p (680 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314078|ref|YP_003778170.1| uncharacterized protein involve... 1077 0.0 gi|300311615|ref|YP_003775707.1| outer membrane biogenesis prote... 355 7e-99 >gi|300314078|ref|YP_003778170.1| uncharacterized protein involved in outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 682 Score = 1077 bits (2785), Expect = 0.0, Method: Compositional matrix adjust. Identities = 542/682 (79%), Positives = 610/682 (89%), Gaps = 2/682 (0%) Query: 1 MSRLTKIFLWLLASLILLIAAAIIFLISFDWNRAKPWINQRVSTAIGRDFAIDGNLSLHW 60 M RLTKI LWL ASLILLI AA+IF++SFDWNRAKPWIN+RVS +IGR+FAIDG+LSLHW Sbjct: 1 MPRLTKIILWLCASLILLITAAVIFILSFDWNRAKPWINERVSASIGREFAIDGDLSLHW 60 Query: 61 QRPEPTSSGAESGFWASWIPWPRLQAENLRLGNPPAFAQSGQMATVQQITFTLNPLPLLV 120 QR + A GFW W+PWPRLQAE LR+GNPPAF+Q+GQMAT+ Q+TFTLNPLPLLV Sbjct: 61 QRAQTLPGAAPPGFWERWLPWPRLQAEQLRIGNPPAFSQAGQMATIGQVTFTLNPLPLLV 120 Query: 121 HRISLPSLEVQDPSVSLLRNREAVNNWTFQSDSTGPSTWQLQLGRISLRQGKLALDDAVD 180 H I+LPSL+++DPSVSLLRN +AVNNWTF+S S GPS W L+LGRI+LRQGKL+LDDAVD Sbjct: 121 HDIALPSLDIRDPSVSLLRNAQAVNNWTFKSSSAGPSAWNLRLGRITLRQGKLSLDDAVD 180 Query: 181 KIKLQGEIDTLDPEREKVYGLGWTLRGSFNGAPVSGQGKAGGVLSLQNQQQPYPLQAQVQ 240 KIKL+ EIDTLDP EK+YGLGW L GSFNGAPVSGQGKAGGVLSLQN QQPYPLQAQV+ Sbjct: 181 KIKLKAEIDTLDPAVEKIYGLGWKLSGSFNGAPVSGQGKAGGVLSLQNVQQPYPLQAQVR 240 Query: 241 VGKTRIGVRGTLTKPAELAALDVRLSLSGASMGNLYPLTGVLLPETPPFATEGRLIGKIA 300 VGKT IGV GTLT+PA LAALD+RLS+SGASMGNLYPLTGVLLPETPPFATEGRLIGKI Sbjct: 241 VGKTAIGVHGTLTRPAALAALDLRLSVSGASMGNLYPLTGVLLPETPPFATEGRLIGKID 300 Query: 301 AGGSQWHYQDFSGKVGDSDLNGSLDYQTGG--TRAQLTGALTSNVLRFDDLAPLIGADSN 358 AG SQW Y+DF GKVG SD+NGSL Y TG R+QLTG+LTS VLRFDDLAPLIGADSN Sbjct: 301 AGHSQWDYEDFRGKVGQSDINGSLRYTTGAGAARSQLTGSLTSEVLRFDDLAPLIGADSN 360 Query: 359 AEKAQRGAEERQPVDKILPVKTFSTEAWGALDADVRFTGKRIIRQQGLPITDLQAHLILK 418 AEKA+RGA+ERQP DK+LPVKTFST+AWGALDADV+ TGKRII+Q+ LPI+DLQAHLILK Sbjct: 361 AEKAERGAQERQPGDKVLPVKTFSTDAWGALDADVKLTGKRIIKQKNLPISDLQAHLILK 420 Query: 419 DKVLTLDPLNFGVAGGNLTSTIRLDGQAARMKADMKLSARKLKIQQLYPKLDASRTSFGE 478 DKVLTL PL+FGVAGGNL STIRLDGQAA MK+++ LSAR+LKIQ+LYP+LDASRTSFGE Sbjct: 421 DKVLTLAPLDFGVAGGNLRSTIRLDGQAAPMKSELILSARRLKIQKLYPQLDASRTSFGE 480 Query: 479 INGDARLSSSGNSVAAMLGSSNGEVKVLVNQGAISKFLLEAAGLNVGNVVISKLFGDKEV 538 INGDARLS++GNSVAAMLGSSNGEVK LV QGAISKFLLEAAGLN+GNVVISKLFGDKEV Sbjct: 481 INGDARLSATGNSVAAMLGSSNGEVKTLVTQGAISKFLLEAAGLNLGNVVISKLFGDKEV 540 Query: 539 KLNCLAGDLPVNDGVMQVKTLLLDTEDALIVVDGSIDMKRELLDLNVHPRSKGLRIISLR 598 KLNCLAGDLPV +G+M +TL+LDTEDALI VDG+IDM+RE LDL+VHP+SKGLRIISLR Sbjct: 541 KLNCLAGDLPVREGIMTARTLMLDTEDALISVDGTIDMRREKLDLHVHPKSKGLRIISLR 600 Query: 599 SPLYVKGSFKAPDVGVDKAVLALRAGGAVALGLLAPVTAILPLINIDGDQKADCGQLLEE 658 SPLYV G+FK PDVGVDK +LALRAG AVALGLLAPVTAILPLIN+DGD++ADC LLEE Sbjct: 601 SPLYVAGTFKQPDVGVDKGMLALRAGSAVALGLLAPVTAILPLINVDGDEQADCAHLLEE 660 Query: 659 VKKAPLLPATGKSKSVKGAPKK 680 +KAP PA GK+ + +PKK Sbjct: 661 ARKAPRAPAAGKTLPAERSPKK 682 >gi|300311615|ref|YP_003775707.1| outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 654 Score = 355 bits (911), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 234/633 (36%), Positives = 340/633 (53%), Gaps = 34/633 (5%) Query: 30 DWNRAKPWINQRVSTAIGRDFAIDGNLSLHWQRPEPTSSGAESGFWASWIPWPRLQAENL 89 DWN AKPW+ +++S A+GRD +DGNL L W + E+G A P RL+AE L Sbjct: 41 DWNSAKPWLGRQLSAALGRDIRVDGNLDLSWSLAQADD---EAGALARLAPQLRLRAERL 97 Query: 90 RLGNPPAFAQSGQMATVQQITFTLNPLPLLVHRISLPSLEVQDPSVSLLRNREAVNNWTF 149 +GNP S + + +PLPLL + L V+ + + R + NW F Sbjct: 98 FIGNPDWAHSSDHLVRAAALDVRFSPLPLLHWHWHVTDLRVEGLDLVMERADDQRKNWRF 157 Query: 150 QSDSTGPSTWQLQLGRISLRQG-------KLALDDAVDKIKLQGEIDTLDPEREKVYGLG 202 S S W + RI L LD D L + + L Sbjct: 158 SSQPR--SRWSFDIHRIVFGHALIRYVELPLDLDLRFDARPLNAT--ATNQKDSAALVLQ 213 Query: 203 WTLRGSFNGAPVSGQGKAGGVLSLQNQQQPYPLQAQVQVGKTRIGVRGTLTKPAELAALD 262 + G + A V+GQ K G + L N+ +PLQA+ +G + + G L P +L+ D Sbjct: 214 AAVTGRYGQAEVNGQIKGGALPDLLNEGSVFPLQAEGDIGGVQTQLSGNLINPHQLSRAD 273 Query: 263 VRLSLSGASMGNLYPLTGVLLPETPPFATEGRL-IGKI--AAGGSQWHYQDFSGKVGDSD 319 +R+ L+G S+ LYP TGV LP T F TEG L I ++ W Y FSGKVG+SD Sbjct: 274 LRIGLAGDSLDRLYPATGVPLPATRAFHTEGALTITRLDPQTKAWDWRYSGFSGKVGESD 333 Query: 320 LNGSLDYQTGGTRAQLTGALTSNVLRFDDLAPLIGADSNAEKAQRGAEERQPVDKILPVK 379 G Y G + L + +LR D P G D++ K P K Sbjct: 334 FAGDAQYVRGPPKNHLKVKAEAGLLRLSDYLP--GDDADQGK---------------PSK 376 Query: 380 TFSTEAWGALDADVRFTGKRIIRQQGLPITDLQAHLILKDKVLTLDPLNFGVAGGNLTST 439 E W LDAD+ KR+ + GL A L LKD+VLT DPL F +AGG Sbjct: 377 PAQPERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNMAGGQGQGQ 436 Query: 440 IRLDGQAARMKADMKLSARKLKIQQLYPKLDASRTSFGEINGDARLSSSGNSVAAMLGSS 499 +RLDG+ +++ +++ + ++++L+P+L + + SF + NG+A LS++GNS AA+L S+ Sbjct: 437 VRLDGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSKFNGEATLSATGNSAAAVLASA 496 Query: 500 NGEVKVLVNQGAISKFLLEAAGLNVGNVVISKLFGDKEVKLNCLAGDLPVNDGVMQVKTL 559 +GE+K + G IS+F+LEAAGLN+ N V +KL+ D++VKL C A D+ + G ++ Sbjct: 497 DGEIKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQHGQATIRHG 556 Query: 560 LLDTEDALIVVDGSIDMKRELLDLNVHPRSKGLRIISLRSPLYVKGSFKAPDVGVDKAVL 619 +L+TEDA I V G +D+ R+ L L++HPR+K +RI+SLR+PL+V+GSF P++G D L Sbjct: 557 ILNTEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPEIGADSGAL 616 Query: 620 ALRAGGAVALGLLAPVTAILPLINIDGDQKADC 652 A RAG A AL L+APV A++PLI+ DC Sbjct: 617 AARAGAAAALALVAPVAAVIPLISPGQAASDDC 649 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,026,179 Number of extensions: 87052 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 2 Length of query: 680 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 588 Effective length of database: 1,184,250 Effective search space: 696339000 Effective search space used: 696339000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)