BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4654.p (472 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrog... 807 0.0 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 159 7e-40 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 155 6e-39 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 154 1e-38 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 149 6e-37 >gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 472 Score = 807 bits (2084), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/472 (87%), Positives = 436/472 (92%) Query: 1 MTDTTVAQLQQLLNRQRQAFAADSDPSHALRMDRLARLLALVDGHEQAIISAIDGDFGGR 60 MTDTT A+LQQL QRQAFAADSDPSHALRMDRLARL AL++ HE A+I+AID DFGGR Sbjct: 1 MTDTTAARLQQLFTAQRQAFAADSDPSHALRMDRLARLQALMEEHETALIAAIDADFGGR 60 Query: 61 SPHETRMAELFVVRAGIAHAQSHLARWMRARRVPTALHFLPGSNRLLAQPLGVVGIISPW 120 SPHETRMAELFV+RAG+ HAQSHL RWMR +RV TALHFLPGSNRLLAQPLGVVG+ISPW Sbjct: 61 SPHETRMAELFVLRAGLRHAQSHLKRWMRPQRVATALHFLPGSNRLLAQPLGVVGVISPW 120 Query: 121 NYPLQLALGPALAALAAGNRVLLKPSELTPLLSALLAELVARYYAPEEMAVVTGDARIGQ 180 NYPLQLALGPALAALAAGNRVLLKPSELTP LSALLAELVARYYAPEEMAVVTGDA IGQ Sbjct: 121 NYPLQLALGPALAALAAGNRVLLKPSELTPRLSALLAELVARYYAPEEMAVVTGDAAIGQ 180 Query: 181 AFAALPLDHLFFTGSTQVGRQVAQLAAANLTPVTLELGGKSPAILDASCHLESSIRRIAF 240 AFAALP DHLFFTGSTQVGRQ+AQLAAANLTPVTLELGGKSPAILD SC L SS++RIAF Sbjct: 181 AFAALPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCRLHSSLQRIAF 240 Query: 241 GKLLNAGQTCVAPDYLLVPRGQAPVVAQRLAQAMSALYPRLHDNPDYTAIISARHRERLL 300 GKLLNAGQTCVAPDYLLVPRGQA VAQ LAQAM LYPRLHDNPDYTAIIS RHR RL Sbjct: 241 GKLLNAGQTCVAPDYLLVPRGQAAGVAQELAQAMGRLYPRLHDNPDYTAIISPRHRARLQ 300 Query: 301 ALVQQARAAGAQVIEVNPAQEDLGHSRKLAPMLILGATTAMQVMQEEIFGPLLPILEYDA 360 ALV++AR GAQVIEVNPA E+LGHSRKLAP LILGAT +M+VMQEEIFGP+LPILEYD Sbjct: 301 ALVEEARGGGAQVIEVNPAGEELGHSRKLAPTLILGATPSMRVMQEEIFGPVLPILEYDQ 360 Query: 361 LEDAIAHVRQGPRPLALYWFGQDAARRDRVLRQTHAGGVTINDCLWHLVQEEQPFGGVGE 420 EDAIA VRQGPRPLALYWFG+DAARR+++LRQTHAGGVTINDCLWHLVQEEQPFGGVGE Sbjct: 361 AEDAIAQVRQGPRPLALYWFGEDAARREQMLRQTHAGGVTINDCLWHLVQEEQPFGGVGE 420 Query: 421 SGMGAYHGRWGFDTFSKRKPVFRQARWNGGALLHPPYGKTFEWVLGLIRRLS 472 SGMGAYHGRWGFDTFSKRKPVFRQAR+NGG LLHPPYGKTFE VLGLIRRLS Sbjct: 421 SGMGAYHGRWGFDTFSKRKPVFRQARFNGGTLLHPPYGKTFERVLGLIRRLS 472 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 159 bits (401), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 136/423 (32%), Positives = 207/423 (48%), Gaps = 24/423 (5%) Query: 31 RMDRLARLLALVDGHEQAIISAIDGDFGGRSPHETRMAELFVVRAGIAH--AQSHLARWM 88 R L +L ALV+ + +++ +D GRSP E RM + V I+H A + A M Sbjct: 68 RGQLLHKLAALVE-RDTDLLADMDARAIGRSPMEPRMMD---VPNAISHLRAAAGWANQM 123 Query: 89 RARRVPTALHF-LPGSNRLLAQPLGVVGIISPWNYPLQLALGPALAALAAGNRVLLKPSE 147 R +PTA + P + + +P+GVVG I PWN PL + A LAAG V++KP+E Sbjct: 124 EGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAE 183 Query: 148 LTPLLSALLAELVARYYAPEE-MAVVTGDAR-IGQAFAALP-LDHLFFTGSTQVGRQVAQ 204 TP + LA L P+ + VVTG +G+A P + + FTGS + GR + + Sbjct: 184 ETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQR 243 Query: 205 LAAANLTPVTLELGGKSPAILDASCHLESSIRRIAFGKLLNAGQTCVAPDYLLVPRGQAP 264 +A VTLELGGKSP I+ E ++ G +N GQ C A +LV R Sbjct: 244 IAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS--- 300 Query: 265 VVAQRLAQAMSALYPRLH-DNPDYTAI----ISARHR-ERLLALVQQARAAGAQVIEVNP 318 +AQR A+A++ + +P + ++ + + ER+ +QQ GA ++ Sbjct: 301 -LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV 359 Query: 319 AQEDLGHSRKLAPMLILGATTAMQVMQEEIFGPLLPILEYDALEDAIAHVRQGPRPLALY 378 ++ D G + P + A M + +EEIFGP+ I+ +D E+AIA LA Sbjct: 360 SRPDKGWF--VQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAAT 417 Query: 379 WFGQDAARRDRVLRQTHAGGVTINDCLWHLVQEEQPFGGVGESGMGAYHGRWGFDTFSKR 438 + + AR RV G V +N W + P+GG+ +SGMG G G +++ Sbjct: 418 VWTTNLARAHRVAAGVKVGAVGVN--CWSPLDANLPWGGIKDSGMGREGGLAGALAYTEE 475 Query: 439 KPV 441 K V Sbjct: 476 KTV 478 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 155 bits (392), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 120/372 (32%), Positives = 186/372 (50%), Gaps = 15/372 (4%) Query: 74 RAGIAHAQSHLARWMRARRVPTALHFLPG---SNRL--LAQPLGVVGIISPWNYPLQLAL 128 R I +A S + W T +P SNR+ + + +GV I+PWN+P + Sbjct: 107 RGEIGYAASFI-EWFAEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMIT 165 Query: 129 GPALAALAAGNRVLLKPSELTPLLSALLAELVARYYAPEEM-AVVTGDAR-IGQAFAALP 186 A ALAAG ++LKP+E TP + LA L R P + +VVTG + IG + P Sbjct: 166 RKAGPALAAGCPMVLKPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNP 225 Query: 187 L-DHLFFTGSTQVGRQVAQLAAANLTPVTLELGGKSPAILDASCHLESSIRRIAFGKLLN 245 + + FTGST VG+ + Q +A+++ ++LELGG +P I+ L++++ K N Sbjct: 226 IVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRN 285 Query: 246 AGQTCVAPDYLLVPRGQAPVVAQRLAQAMSALYPRLHDNPDYTA--IISARHRERLLALV 303 AGQTCV + + V G A +L +A+ T +I+ + +++ V Sbjct: 286 AGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHV 345 Query: 304 QQARAAGAQVIEVNPAQEDLGHSRKLAPMLILGATTAMQVMQEEIFGPLLPILEYDALED 363 A A GA+V+ + + LGHS P ++ T AMQV +EE FGP+ P+ + E+ Sbjct: 346 ADAVAKGARVL-LGGKRHALGHSF-FEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEE 403 Query: 364 AIAHVRQGPRPLALYWFGQDAARRDRVLRQTHAGGVTINDCLWHLVQEEQPFGGVGESGM 423 +A LA Y++ +D R RV +G V IN L + E PFGGV +SG+ Sbjct: 404 VLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGL--ISNEVAPFGGVKQSGL 461 Query: 424 GAYHGRWGFDTF 435 G +G D + Sbjct: 462 GREGSHYGIDDY 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 154 bits (390), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 119/350 (34%), Positives = 176/350 (50%), Gaps = 15/350 (4%) Query: 102 GSNRLLAQPLGVVGIISPWNYPLQLALGPALAALAAGNRVLLKPSELTPLLSALLAELVA 161 G+ ++ +P+GV +I+PWN+P+ ALAAG V+LKPSEL+PL + L AE+V Sbjct: 139 GTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLFAEIVH 198 Query: 162 RYYAPEEM-AVVTGD-ARIGQAFAALP-LDHLFFTGSTQVGRQVAQLAAANLTPVTLELG 218 P + +V G A +G A +A P +D + TGST+ G VAQ AA + V ELG Sbjct: 199 EAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELG 258 Query: 219 GKSPAILDASCHLESSIRRIAFGKLLNAGQTCVAPDYLLVPRGQAPVVAQRLAQAMSALY 278 GKSP ++ LE +I L N GQ+C AP L+VPR +A+ A++ L Sbjct: 259 GKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRA---ALARVHELALATLA 315 Query: 279 PRLHDNPDYTAIISA--RHRERLLALVQQARAA---GAQVIEVNPAQED-LGHSRKLAPM 332 +P+ A +R + L + Q A GA+++ P + D L P Sbjct: 316 QMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPT 375 Query: 333 LILGATTAMQVMQEEIFGPLLPILEYDALEDAIAHVRQGPRPLALYWFGQDAARRDRVLR 392 + T M + QEEIFGP+L IL YD +E+AIA L + G+D + V Sbjct: 376 IFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAA 435 Query: 393 QTHAGGVTINDCLWHLVQEEQPFGGVGESGMGAYHGRWGFDTFSKRKPVF 442 + +G V +N W + PFGG +SG G +G G + + + K + Sbjct: 436 RIQSGQVHLNYPAW---DPQAPFGGYKQSGNGREYGVEGMEEYMEVKAIL 482 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 149 bits (375), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 112/346 (32%), Positives = 170/346 (49%), Gaps = 21/346 (6%) Query: 109 QPLGVVGIISPWNYPLQLALGPALAALAAGNRVLLKPSELTPLLSALLAELVARYYAPEE 168 +PLGVVG I PWN+P+ +A+ ALAAGN V+LKP+E TP +L EL+A P Sbjct: 153 EPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLIELIADLIPPGV 212 Query: 169 MAVVTG-DARIGQAFAALP-LDHLFFTGSTQVGRQVAQLAAANLTPVTLELGGKSPAILD 226 + +V G G+ A+ + + FTG T GR + Q A+ NL PVTLELGGKSP I Sbjct: 213 VNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFF 272 Query: 227 ASCHLESSIRRI----AFGKL-LNAGQTCVAPDYLLVPRGQAPVVAQRLAQAMSALYPRL 281 A + F LN G+ C P +LV Q + + + +A+ + Sbjct: 273 ADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLV---QESIYERFIERALKRVAAIK 329 Query: 282 HDNP-DYTAIISAR----HRERLLALVQQARAAGAQVIEVNPAQE---DLGHSRKLAPML 333 NP D + +I A+ E++L+ + + GA+V+ +E DL + P + Sbjct: 330 QGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPTV 389 Query: 334 ILGATTAMQVMQEEIFGPLLPILEYDALEDAIAHVRQGPRPLALYWFGQDAARRDRVLRQ 393 G M++ QEEIFGP++ + + E+A+A L + +D R R+ R+ Sbjct: 390 FQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGRE 448 Query: 394 THAGGVTINDCLWHLVQEEQPFGGVGESGMGAYHGRWGFDTFSKRK 439 AG V N +HL FGG +SG+G + + D + + K Sbjct: 449 IQAGRVWTN--CYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTK 492 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,387,383 Number of extensions: 57633 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 20 Length of query: 472 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 383 Effective length of database: 1,198,455 Effective search space: 459008265 Effective search space used: 459008265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)