BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4652.p (195 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspi... 225 2e-60 >gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 225 bits (573), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 121/189 (64%), Positives = 131/189 (69%), Gaps = 36/189 (19%) Query: 6 VIVGGGSAGA----TLSGIGRPEDILPHGIALQHALPGVGQNLQDHLDFTQG-------- 53 VI+ G+ G+ LSG+GR +DILPHGIAL H LPGVGQNLQDHLDFTQG Sbjct: 255 VIIAAGAFGSPQLLQLSGVGRSDDILPHGIALHHPLPGVGQNLQDHLDFTQGWTTRDTDN 314 Query: 54 ------------------------LIATPFAEGAAFLKTHAELERPDIQLHFCIAIVDDH 89 LIATPFAEGAAFLKT EL+RPDIQLHFCIAIVDDH Sbjct: 315 FGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTRPELDRPDIQLHFCIAIVDDH 374 Query: 90 ARKLHAGYGFSLHMCMLRPHPRGRVGLQSADPLADPLIDAGYLSDPRDLLTMIAGANSSA 149 ARKLHAGYGFSLHMCMLRPH RGRVGLQSADP+ADPLID GYLSDPRDL TMI GA + Sbjct: 375 ARKLHAGYGFSLHMCMLRPHSRGRVGLQSADPMADPLIDPGYLSDPRDLATMIDGARMAR 434 Query: 150 ATTIWARIR 158 + +R Sbjct: 435 QIVMTEPLR 443 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.140 0.476 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 755,911 Number of extensions: 33097 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 2 Length of query: 195 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 115 Effective length of database: 1,241,070 Effective search space: 142723050 Effective search space used: 142723050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)