BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4650.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 664 0.0 gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 135 4e-33 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 126 2e-30 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 77 2e-15 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 664 bits (1712), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/387 (89%), Positives = 359/387 (92%), Gaps = 2/387 (0%) Query: 1 MNPSVPAGAAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLDHLLMAH 60 MNPSVPAG APIPVSLLTGFLGSGKTTLLNHLITQPEMK+TLVIINEFGEIGLDHLL+AH Sbjct: 1 MNPSVPAGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAH 60 Query: 61 SQEDTVVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTGLASPTPILHT 120 SQE+T+VEMSSGCLCCTIRGDLKKTLKDITWRFAEGG+RKF+RVVIETTGLASP PILHT Sbjct: 61 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 Query: 121 LMTDPFIAARYRLDGVIVTVDAVNGMSTLDHHPEAIQQAAVADRLLLTKTDLADAATLAA 180 LMTD FIA RYRLDGVIVTVDAVNGMSTLDHH EA+QQAAVADRLLLTKTDL DAATLAA Sbjct: 121 LMTDAFIAERYRLDGVIVTVDAVNGMSTLDHHEEAVQQAAVADRLLLTKTDLVDAATLAA 180 Query: 181 LQARLRTLNPGARQLQPQLGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQQAHAHE 240 LQARLRTLNPGARQLQPQ+GQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQ H H Sbjct: 181 LQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQARHGHG 240 Query: 241 HHHHGHDHHDHHDHGHAHHEHHDHHDHAHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSL 300 HH H H H HGH H H HDH HDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSL Sbjct: 241 HHG--HHHGHDHHHGHDHGHDHHGHDHEHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSL 298 Query: 301 LVGFKGPNILRIKGILNLKGDDKPTVIHGVQHIFHPTATLPEWPSEDRRSRIVFITRDIE 360 LVGFKGPNILRIKGILNLKG+ PTVIHGVQHIFHPTATLP+WPSEDRRSRIVFITRD+E Sbjct: 299 LVGFKGPNILRIKGILNLKGEALPTVIHGVQHIFHPTATLPQWPSEDRRSRIVFITRDVE 358 Query: 361 RATIERSFDAFMQAQAIEREARERQQA 387 RATIERSFDAFMQAQAIEREARE++ A Sbjct: 359 RATIERSFDAFMQAQAIEREAREKKAA 385 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 135 bits (341), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 85/246 (34%), Positives = 133/246 (54%), Gaps = 36/246 (14%) Query: 12 IPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLDHLLMAHS------QEDT 65 +PV++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D L+ E+ Sbjct: 13 LPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLARTEEK 72 Query: 66 VVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTGLASPTPILHTL-MTD 124 +VEMS+GC+CCT+R DL ++ + +R+FD++VIE+TG++ P P+ T TD Sbjct: 73 LVEMSNGCICCTLREDLLMEVRKL------ARERRFDQLVIESTGISEPLPVAETFTFTD 126 Query: 125 ---PFIAARYRLDGVIVTVDAVNGMS------TLDHHPEA-------------IQQAAVA 162 ++ RLD ++ VDA N + +LD ++ I+Q Sbjct: 127 EDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIEQIEFC 186 Query: 163 DRLLLTKTDLADAATLAALQARLRTLNPGARQLQPQLGQIAAAELLDAGLFKPGEKMPDV 222 D ++L K DL A L L A L LNP AR ++ + G++A ++LD GLF E+ Sbjct: 187 DVIVLNKLDLVAADDLERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLFD-FEQASQA 245 Query: 223 ARWLNE 228 WL E Sbjct: 246 PSWLQE 251 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 88/253 (34%), Positives = 136/253 (53%), Gaps = 39/253 (15%) Query: 1 MNPSVP-----AGAAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLDH 55 MN P A A IPV++L+GFLGSGKTTLLNH++ VI+N+ E+ +D Sbjct: 1 MNEPAPEVLLNAQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDG 60 Query: 56 LLMAHSQE-----DTVVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTG 110 + + E D +VEMS+GC+CCT+R DL + + + A G +FD ++IE+TG Sbjct: 61 DDVRRNTELYRGNDELVEMSNGCICCTLRADLLEQVSAL----ARSG--RFDYLLIESTG 114 Query: 111 LASPTPILHT---LMTDPF-IAARYRLDGVIVTVDA---------------VNGMSTLDH 151 ++ P P+ T L +D F ++ RLD ++ V+ +G ST Sbjct: 115 ISEPIPVAETFAFLDSDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPL 174 Query: 152 HPEAIQQAAVADRLLLTKTDLADAATLAALQARLRTLNPGARQLQPQLGQIAAAELLDAG 211 I+Q A+ +L+++ DL DA+ A+L+A L+ LNP AR L G++ ++LD Sbjct: 175 SDLLIEQVEYANVILVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTR 234 Query: 212 LFKPGEKMPDVAR 224 LF +P +AR Sbjct: 235 LF----DLPSLAR 243 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 64/210 (30%), Positives = 100/210 (47%), Gaps = 17/210 (8%) Query: 1 MNPSVPAGAAPIPVSLLTGFLGSGKTTLLNHLITQPEMKNTLVIINEFGEIGLD-HLLMA 59 + P++P PIPV+++TGFLG+GKTTLL L+ + + + ++INEFGE+ +D LL A Sbjct: 10 LAPALP----PIPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLLRA 65 Query: 60 HSQEDTVVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGQRKFDRVVIETTGLASPTPILH 119 V++ GD + + A Q D V+IET+GLA P+ ++ Sbjct: 66 EGDAQGQVQIQDFAHGLIAYGDDAHFVPAMLALAARRAQ--IDHVLIETSGLALPSAVME 123 Query: 120 TLMTDPFIAARYRLDGVIVTVDAVNGMSTLDHHPEAI---------QQAAVADRLLLTKT 170 L +A + LD + VD + +A+ QQ AD ++L K Sbjct: 124 VLQGAE-MARHFVLDATLAVVDTPLLLEDAFASADAVGAATAQLFRQQLECADVVVLNKI 182 Query: 171 DLADAATLAALQARLRTLNPGARQLQPQLG 200 D + A L + +R L P R L+ G Sbjct: 183 DGMEEAPLLQAEQAVRALAPNVRFLELAYG 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,076,841 Number of extensions: 40168 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 4 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)