BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4641.p (418 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 725 0.0 gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 399 e-112 gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 388 e-109 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 79 7e-16 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 70 3e-13 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 725 bits (1871), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/421 (90%), Positives = 404/421 (95%), Gaps = 3/421 (0%) Query: 1 MTQTAALSA--TTLDTLPPPSSTPP-RLVARIRDDAQALASARQLAEDFRGEAALRDRER 57 MTQTAA A TTLD+ PPP S+ P RLVARIRDDA+A+A+AR+LAEDF EAALRDRER Sbjct: 1 MTQTAAAHASLTTLDSFPPPPSSTPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRER 60 Query: 58 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATLSAADGSIGQIPQNHFYAVE 117 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIAT+SAADGSIGQIPQNHFYAVE Sbjct: 61 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE 120 Query: 118 VIRVNGSDAQKRFFFEQVLQGQRFGNALAELGTKTSHDRKTRLTRNEHGPGYRISGRKFY 177 +IRVNGS++QKRFFFE+VLQG RFGNALAELGTKTSHDRKTRL+RN HGPGYRI GRKFY Sbjct: 121 LIRVNGSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFY 180 Query: 178 ATGALFADWVPTSVIDEDGVQQLAIVPRHATGLTIIDDWSGFGQRTTGSGSAVFENVHVP 237 ATGAL+ADWVPTSVID+DGVQQLAIVPRHA GLTIIDDWSGFGQRTTGSGSAVFENVHVP Sbjct: 181 ATGALYADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVP 240 Query: 238 AEAVIGFASAFTRPTQLGPMAQLQHAAIDLGIARAAYADLLEFVRTRSRAWVDSGVQRAA 297 A+AVIGFA+AF+RPTQ+GPMAQ+QHAAIDLGIARAAYADLL FVRTRSRAWVDSGV+RA Sbjct: 241 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAV 300 Query: 298 DDPLTIREVGDLTIQLHAAEALVRRAARKVEAAAAAPDEDSVAAASIAVAEARVLTTEVS 357 DDPLTIREVGDLTIQLHAAEAL+RRA RKV+AAAAAPDEDSVAAASIAVAEARVLTTEVS Sbjct: 301 DDPLTIREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVS 360 Query: 358 LAAGSKLFELAGSQSTLSEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHGA 417 LAAGSKLFELAGSQSTL+EHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHGA Sbjct: 361 LAAGSKLFELAGSQSTLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHGA 420 Query: 418 I 418 I Sbjct: 421 I 421 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 399 bits (1024), Expect = e-112, Method: Compositional matrix adjust. Identities = 207/400 (51%), Positives = 272/400 (68%), Gaps = 5/400 (1%) Query: 17 PPSSTPPRLVARIRDDAQALASARQLAEDFRGEAALRDRERKLPWDELERYVRSGLWGIT 76 P +S P + IRDDA+A+A A LA A+ RD+ R+ P +E+E Y +SGLW I Sbjct: 23 PRASQPAHV---IRDDAEAIAVAHALAPRLAQGASERDQLRRWPVEEIEAYSQSGLWAIN 79 Query: 77 VPKEYGGAGVSYGTLAEVIATLSAADGSIGQIPQNHFYAVEVIRVNGSDAQKRFFFEQVL 136 VP+ +GGA VSY TLAEVIA +SAAD S+GQI QNH V +R Q+R FF L Sbjct: 80 VPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVRTVSDPEQQRRFFADFL 139 Query: 137 QGQRFGNALAELGTKTSHDRKTRLTRNEHGPGYRISGRKFYATGALFADWVPTSVIDEDG 196 +G R+GNA +E G+K + D +TR + G ++G+KFY+TGAL A +VP DE+ Sbjct: 140 RGVRWGNAFSESGSKRAADFETRFV--DAGDHVIVTGKKFYSTGALLAHYVPIVANDEEN 197 Query: 197 VQQLAIVPRHATGLTIIDDWSGFGQRTTGSGSAVFENVHVPAEAVIGFASAFTRPTQLGP 256 + R+A GLT+IDDWS FGQRTT SG+ + + V VP +I + P+ G Sbjct: 198 RTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPAYKGYLEPSADGA 257 Query: 257 MAQLQHAAIDLGIARAAYADLLEFVRTRSRAWVDSGVQRAADDPLTIREVGDLTIQLHAA 316 + Q+ AA+D+GIARAA AD +FVRT+SR W+DS A +DP +I+ +GDL I+LHA+ Sbjct: 258 IFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYSIQAIGDLQIRLHAS 317 Query: 317 EALVRRAARKVEAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLSE 376 EAL+ RA R ++AA AAPD SVA A I AEA+VL+TE+++ A +KLFELAG++STL + Sbjct: 318 EALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQ 377 Query: 377 HNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHG 416 +NLDRHWRNARTHTLHDPVRWKY +GNY LNG KPP H Sbjct: 378 YNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHA 417 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 388 bits (996), Expect = e-109, Method: Compositional matrix adjust. Identities = 209/394 (53%), Positives = 274/394 (69%), Gaps = 2/394 (0%) Query: 24 RLVARIRDDAQALASARQLAEDFRGEAALRDRERKLPWDELERYVRSGLWGITVPKEYGG 83 R RI ++ AL A +LA +F AA RDRER LP++++ER+ +SGLW ITVP+ +GG Sbjct: 15 RPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQSGLWAITVPRSHGG 74 Query: 84 AGVSYGTLAEVIATLSAADGSIGQIPQNHFYAVEVIRVNGSDAQKRFFFEQVLQGQRFGN 143 G S+ T+AEVI +SAAD SIGQIPQNHF + +I S Q+R FF +VL G R GN Sbjct: 75 IGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASQEQQRLFFAEVLDGARLGN 134 Query: 144 ALAELGTKTSHDRKTR--LTRNEHGPGYRISGRKFYATGALFADWVPTSVIDEDGVQQLA 201 A E K D +TR L R Y ++G +FY+TGAL+A W+P+ ID+DG + Sbjct: 135 AGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRAIDDDGQPLVV 194 Query: 202 IVPRHATGLTIIDDWSGFGQRTTGSGSAVFENVHVPAEAVIGFASAFTRPTQLGPMAQLQ 261 R A G+ +IDDWS FGQRTT SG+ VF+NV VPA V+ A +P +GP++QL Sbjct: 195 YTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDALQQPNNIGPVSQLI 254 Query: 262 HAAIDLGIARAAYADLLEFVRTRSRAWVDSGVQRAADDPLTIREVGDLTIQLHAAEALVR 321 AAID GIA AA D + FV TRSR +VDSG++RA+DDP +R++G+L+++LHAA+AL+ Sbjct: 255 QAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLRLHAADALLE 314 Query: 322 RAARKVEAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLSEHNLDR 381 AA +++ A + +A AS AVAEA+VL+TE++L A KLFEL+GS +TL+ HNLDR Sbjct: 315 DAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTELALLASEKLFELSGSAATLASHNLDR 374 Query: 382 HWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRH 415 HWRNAR HTLHDPVRWKY A+GNY LNG PPRH Sbjct: 375 HWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRH 408 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 79.0 bits (193), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 87/354 (24%), Positives = 152/354 (42%), Gaps = 26/354 (7%) Query: 50 AALRDRERKLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIAT--LSAADGSIGQ 107 AA DR + P D ++ G+ GITV +EYGGAG+ Y LA +IA +S A S+G Sbjct: 34 AAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGY--LAHIIAMEEISRASASVGL 91 Query: 108 IPQNHF-YAVEVIRVNGSDAQKRFFFEQVLQGQRFGNALAELGTKTSHDRKTRLTRNEHG 166 H V I+ NG++ QKR + +++ G G S +L ++ G Sbjct: 92 SYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDVVSMKLRADKKG 151 Query: 167 PGYRISGRKFYATGALFAD----WVPTSVIDEDGVQQLA--IVPRHATGLTIIDDWSGFG 220 Y ++G K + T AD + T + E G + + +V + G ++ G Sbjct: 152 DRYVLNGSKMWITNGPDADVLVVYAKTDL--EAGARGMTAFLVEKGYKGFSVAQKLDKLG 209 Query: 221 QRTTGSGSAVFENVHVPAEAVIGFASAFTRPTQLGPMAQLQHAAID---LGIARAAYADL 277 R + +G VF++ VP E V+G G + + + LGI +A + Sbjct: 210 MRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSG--LDFERSVLSGGPLGIMQACMDVV 267 Query: 278 LEFVRTRSRAWVDSGVQRAADDPLTIREVGDLTIQLHAAEALVRRAARKVEAAAAAPDED 337 + +V R + Q + L ++ D+ + A +A V + + A +A D Sbjct: 268 VPYVHDRKQFG-----QAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSA---D 319 Query: 338 SVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLSEHNLDRHWRNARTHTL 391 V A A A + + E + + + G ++E+ + R WR+A+ + + Sbjct: 320 KVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEI 373 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 70.1 bits (170), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 83/349 (23%), Positives = 147/349 (42%), Gaps = 26/349 (7%) Query: 54 DRERKLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATLSAADGSIGQI--PQN 111 DR P + L+ G G+ VP+++GGAG+ Y +L + ++A DG+ I QN Sbjct: 30 DRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQN 89 Query: 112 HFYAVEVIRVNGSDAQKRFFFEQVLQGQRFGN-ALAELGTKTSHDRKTRLTRNEH-GPGY 169 A + GS QK + + + +G+ G L E T D TR E G + Sbjct: 90 SL-ACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTE--PHTGSDAAAIRTRAERDGDDF 146 Query: 170 RISG-RKFYATGALFADWVPTSVIDEDGVQQ---LAIVPRHATGLTIIDDWSGFGQRTTG 225 ++G ++F +G + +V D ++ +VP G + GQ + Sbjct: 147 ILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTPGFVVGRSEEKMGQHASD 206 Query: 226 SGSAVFENVHVPAEAVIGFASAFTRPTQLGPMA-QLQHAAIDLGIARAAYADLLEFVRTR 284 + + +N VPA A++G R A ++ AA +G+ARAA+ + + R R Sbjct: 207 TVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQR 266 Query: 285 SRAWVDSGVQRAADDPLTIREVGDLTIQLHAAEALVRRAARKVEAAAAAPDEDSVAAASI 344 GV + R + D+ L AA +V RAA+ + E S+ Sbjct: 267 E----SFGVPIIEHQAVNFR-LADMNTLLDAARLMVWRAAQLKDQGRPCLKEASM----- 316 Query: 345 AVAEARVLTTEVSLAAGSKLFELAGSQSTLSEHNLDRHWRNARTHTLHD 393 A++ +E + S ++ G S+ ++R +R+ R +++ Sbjct: 317 ----AKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYE 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,207,601 Number of extensions: 49995 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 6 Length of query: 418 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 330 Effective length of database: 1,203,190 Effective search space: 397052700 Effective search space used: 397052700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)