BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4635.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311223|ref|YP_003775315.1| sigma-54 dependent transcriptio... 89 9e-19 >gi|300311223|ref|YP_003775315.1| sigma-54 dependent transcription activator protein [Herbaspirillum seropedicae SmR1] Length = 675 Score = 88.6 bits (218), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 71/232 (30%), Positives = 101/232 (43%), Gaps = 14/232 (6%) Query: 78 CLSMLHQTVREADYCVMLTNAGGTTIDYRVDRERRQDFKRAGLYLGSCWSESEEGTCGVA 137 + L+ +R V+L++A GT + D + R L G WSE GT + Sbjct: 78 VMETLYDQIRNTHSMVILSDAEGTILHTLGDSDFLAKADRVALGPGGRWSEQLRGTNAIG 137 Query: 138 AVLQDALPITVHKSDHFRAAFTTLTCSAAPIFSPAGELIGVLDASAIRSPDERDSQNLVN 197 L + P TVH H+ A LTCSAAPIF G+++GVLD S + + L Sbjct: 138 TALFEQKPTTVHAQQHYLDANRFLTCSAAPIFDHRGQVLGVLDVSGECGSFHKHTMAL-- 195 Query: 198 RIVQQSATLIEDGYFLNAMACAWVLLVHRSRHYVEAQPEILIAFDALGNIIAANRRAREC 257 V+ SA +IE+ + L H +V E +++F G +AANR A+ Sbjct: 196 --VRMSAQMIENHLLAGSFPDCITLHFHSRAEFVGTLVEGIVSFTPGGRFLAANRSAQFQ 253 Query: 258 IDGL-------HCLPRPLGEVFDLHFERLLDSRALQGLHLLRLHGGATLYGR 302 + GL H G L FE +A GL L LH G ++GR Sbjct: 254 L-GLTLAALQSHTFASLFGVPLSLLFEH--HRKAAPGLLELCLHNGIRIHGR 302 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,032,986 Number of extensions: 37659 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 1 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)