BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4624.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 822 0.0 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 293 3e-80 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 120 2e-28 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 112 7e-26 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 108 1e-24 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 822 bits (2123), Expect = 0.0, Method: Compositional matrix adjust. Identities = 440/475 (92%), Positives = 459/475 (96%) Query: 1 MSSQPSSSGAPAPQPHIVIAGEGLPPERRGRAILALGIAVGLATLDTAIANTALPAMALD 60 MSSQP++ G AP PHIVIAGEGLPPE+RGRAILALGIAVGLATLDTAIANTALPAMALD Sbjct: 1 MSSQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD 60 Query: 61 LKTTPAASVWIVTAYQLAMMVALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLP 120 L+TTPAASVWIVTAYQLAMMVALLPLAALGEI+GYRRIYIWGLVLFTAASLLCAVAWSLP Sbjct: 61 LQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLP 120 Query: 121 TLVIARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 TLV+ARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA Sbjct: 121 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 Query: 181 IAPWPWLFAVNVPLGVAAVILALPSLPHTKLSSHAFDLRSAVLNAAAFGLLILAIGEGAH 240 IAPWPWLFAVNVPLGV AV+LA+PSLPHT L+ HAFDLRSA+LNAAAFGLLILAIGEGAH Sbjct: 181 IAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH 240 Query: 241 QAELKVVGLELAAAIVCGLLLLKRELSHPAPMLPVDLFRHPMFTLSAITAVCSFAAQGLA 300 QA+ VVGLELAAAI+CGL LLKRELSHPAPMLPVDLFRHPMF+LSA+TAVCSFAAQGLA Sbjct: 241 QAQASVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLA 300 Query: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRLSDHYPVGILGGVGLAILCVG 360 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGR+SD YPVG+LGGVGLA+LC G Sbjct: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAG 360 Query: 361 LLWMTFMPAAPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL 420 LL MTFMPA+PSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL Sbjct: 361 LLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL 420 Query: 421 GQSVGAALVALCFNLSETHGSRLALGFGAAFAGVACIASFLRLLTRNPYAPPLKK 475 GQSVGAALVALCFNLS+T GSRLALGFGAAFAG ACIASFLRLLTRNPYAPP KK Sbjct: 421 GQSVGAALVALCFNLSQTQGSRLALGFGAAFAGAACIASFLRLLTRNPYAPPPKK 475 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 293 bits (749), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 176/416 (42%), Positives = 259/416 (62%), Gaps = 8/416 (1%) Query: 22 EGLPPERRGRAILALGIAVGLATLDTAIANTALPAMALDLKTTPAASVWIVTAYQLAMMV 81 EGLP ++R RA+LA+ A +A D I N ALP ++ L+T+ A S+W+ AY LA + Sbjct: 20 EGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAM 79 Query: 82 ALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLPTLVIARFLQGLGGAGIMSVNT 141 L AAL +G+R + GL +FT ASL CA++ S+ L++ R LQGLGGA ++S+ Sbjct: 80 TLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAP 139 Query: 142 ALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVAAVIL 201 A+ R I+P R LGR +GLN ++VA + VGP + +LA+ W W+FA+NVPLGV AV L Sbjct: 140 AIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVWL 199 Query: 202 ALPSLPHTKLSSHA-FDLRSAVLNAAAFGLLILAIGEGAHQAE-------LKVVGLELAA 253 A ++P T+ + A FDL A+ +A A G LI+A A AE Sbjct: 200 AWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAAYAVT 259 Query: 254 AIVCGLLLLKRELSHPAPMLPVDLFRHPMFTLSAITAVCSFAAQGLAFVSLPFFFHHDLG 313 A++ GL ++ + P+LP+D+F +P F+L+A+T++ SF QG+AFV+LPF F + G Sbjct: 260 ALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYG 319 Query: 314 RSQVEIGLLMTPWPVAVAIMAPIAGRLSDHYPVGILGGVGLAILCVGLLWMTFMPAAPSV 373 + E L TPWP+ + ++AP AGRL+D + +L +GLA+ +GL+ + +P Sbjct: 320 YTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLPDQAQP 379 Query: 374 WEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALV 429 W+I WR L+CGMGFGFFQSPN + ++ + RSG ASG++A R GQ +GAAL+ Sbjct: 380 WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALL 435 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 108/344 (31%), Positives = 163/344 (47%), Gaps = 7/344 (2%) Query: 33 ILALGIAVGLATLDTAIANTALPAMALDLKTTPAASVWIVTAYQLAMMVALLPLAALGEI 92 ++AL I +A LD + N L +A L + VWIV Y L LL AL + Sbjct: 32 LVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADR 91 Query: 93 VGYRRIYIWGLVLFTAASLLCAVAWSLPTLVIARFLQGLGGAGIMSVNTALIRFIY-PTR 151 G RR+Y+ GL LF ASLLC A + P L++AR LQGLG A M + +L+ +Y R Sbjct: 92 FGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDR 151 Query: 152 SLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVAAVILALPSLPHTKL 211 R +G+ + V +A VGP V ++ W +F +NVP+G+ +++A +P Sbjct: 152 VRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGG 211 Query: 212 SSHAFDLRSAVLNAAAF-GLLILAIGEGAHQAELKVVGLELAAAIVCGLLLLKRELSHPA 270 + A +L S + GL + I + + LA A+ G L++ EL A Sbjct: 212 HARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRHELRGIA 271 Query: 271 PMLPVDLFRHPMF-TLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVA 329 P+LP LF P F + I + +F G F L F G ++ GL + P Sbjct: 272 PLLPKALFATPQFAAANGIGFLINFGVYGNLFY-LALFLQQGRGADALQTGLQLVPMMAV 330 Query: 330 VAIMAPIAGRLSDHY--PVGILGGVGLAILCVGLLWMTFMPAAP 371 + ++GR+S H+ V +L G+ + + L M F PA P Sbjct: 331 IFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALA-MGFSPATP 373 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 112 bits (280), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 100/409 (24%), Positives = 180/409 (44%), Gaps = 18/409 (4%) Query: 42 LATLDTAIANTALPAMALDLKTTPAASVWIVTAYQLAMMVALLPLAALGEIVGYRRIYIW 101 +A LDT IAN ++ +A L + + +++T+Y +A + + L G R++I Sbjct: 44 IAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFIL 103 Query: 102 GLVLFTAASLLCAVAWSLPTLVIARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNT 161 +V+F S LC +A SL L+ R LQGL G +M ++ L+ I+P VGL Sbjct: 104 SMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWA 163 Query: 162 LIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVAAVILALPSLP--HTKLSSHAFDLR 219 + +A GP + + WP++F +NVP+ + + L TK+S D Sbjct: 164 MTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRV 223 Query: 220 SAVLNAAAFGLLILAIGEGAHQAELKVVGLELAA--AIVCGLLLLKRELSHPAPMLPVDL 277 VL L L + EG + + L A A+V + L EL+ P++ + + Sbjct: 224 GLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRV 283 Query: 278 FRHPMFTLSAITAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIA 337 FRH FT S T +F A + V P + +G + G + + + AP+ Sbjct: 284 FRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMV 343 Query: 338 GRLSDHYPVGILGGVGLAILCVGLLWMTFM-------PAAPSVWEISWRMLLCGMGFGFF 390 ++S ++C+G+ W+ F+ S ++I W + L G+G F Sbjct: 344 AKMSTTID-------ARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLF 396 Query: 391 QSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALVALCFNLSETH 439 P ++S + A+G+++ R + ++ ++V + T+ Sbjct: 397 FVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATY 445 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 114/443 (25%), Positives = 199/443 (44%), Gaps = 21/443 (4%) Query: 1 MSSQPSSSGAPAPQPHIVIAGEGLPPERRGRAILALGIAVGLATLDTAIANTALPAMALD 60 M++ P+ S A P P + A L L++ +AV + LD++IAN A+P ++ + Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVL-------GTLSVSLAVFMNVLDSSIANVAIPTISGN 53 Query: 61 LKTTPAASVWIVTAYQLAMMVALLPLAALGEIVGYRRIYIWGLVLFTAASLLCAVAWSLP 120 L + W++T++ A +++ L + +G R+++ ++LF AS LC +A +LP Sbjct: 54 LGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLP 113 Query: 121 TLVIARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 L+ AR LQGL ++ ++ +L+ YP + L + +A GP++ I Sbjct: 114 ILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITD 173 Query: 181 IAPWPWLFAVNVPLGV--AAVILAL---PSLPHTKLSSHAFDLRSAVLNAAAFGLLILAI 235 WPW+F +N+P+G+ A V LA+ P KL L V AAF +++ Sbjct: 174 SYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKG 233 Query: 236 GEGAHQAELKVVGLELAAAIVCGLLLLKRELSHPAPMLPVDLFRHPMFTLSAITAVCSFA 295 + A +V L + A I ++ EL++ P++ + LF F I + Sbjct: 234 KDLDWFASPTIVTLGIIALIAFAYFVV-WELNNDHPVVDLRLFGRRNFAGGTIAISIGYG 292 Query: 296 AQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGR---LSDHYPVGILGGV 352 V LP + LG ++ GL P + I+ P+ GR L D V + Sbjct: 293 VFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFI 352 Query: 353 GLAILCVGLLWM-TFMPAAPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGAS 411 G AI + W+ + + LL G+ F P L++ PP R A+ Sbjct: 353 GFAI----VFWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAA 408 Query: 412 GIVATARLLGQSVGAALVALCFN 434 G+ + R+ +VG ++ +N Sbjct: 409 GLSSFVRVFCGAVGTSISTTAWN 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,295,760 Number of extensions: 50083 Number of successful extensions: 386 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 369 Number of HSP's successfully gapped: 17 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)