BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4622.p (495 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314018|ref|YP_003778110.1| two component sensor histidine ... 877 0.0 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 88 1e-18 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 87 2e-18 gi|300313022|ref|YP_003777114.1| two component sensor histidine ... 82 8e-17 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 82 9e-17 >gi|300314018|ref|YP_003778110.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 492 Score = 877 bits (2267), Expect = 0.0, Method: Compositional matrix adjust. Identities = 452/495 (91%), Positives = 473/495 (95%), Gaps = 3/495 (0%) Query: 1 MSNAIEHTHPPHSPHLPSIRKPGYLGLSFRQLLLAAFLLIAALLSGTSIHALFTLDRMSS 60 M NAIEH HP +PH R GYLGLSFRQLLLAAFLLIAALLSGTSIHALFTLDRMS+ Sbjct: 1 MLNAIEH-HP--TPHAAPARPSGYLGLSFRQLLLAAFLLIAALLSGTSIHALFTLDRMSA 57 Query: 61 NSRETARQAVQLTESAQRLAERTVAMERSARQYLVLDDPAFHARFNEAREQARQALGELS 120 NSRETARQAVQLTE+AQRLAERTVAMERSARQYLVLDDPAFH RFNEAR+QARQALGEL+ Sbjct: 58 NSRETARQAVQLTEAAQRLAERTVAMERSARQYLVLDDPAFHDRFNEARDQARQALGELA 117 Query: 121 DSLPMAPRELFSQWSLYGDEAASVLEAESRKDKGEQAKLFQDFARLPALNERIALESRRE 180 DSLP APRE+F QWS+Y DEAA VL+A+SRKDKGEQA+LFQDFARLPALNERIALESRRE Sbjct: 118 DSLPKAPREMFGQWSVYVDEAAGVLDADSRKDKGEQARLFQDFARLPALNERIALESRRE 177 Query: 181 VDRRNNALSAALDQQRQLLTAQVLGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRF 240 VDRRNNALSAAL+QQRQ+LTAQV+GAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRF Sbjct: 178 VDRRNNALSAALEQQRQMLTAQVVGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRF 237 Query: 241 DQPIEVRGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDD 300 DQPI+VRGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDD Sbjct: 238 DQPIDVRGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDD 297 Query: 301 GVAGQLNDNQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVID 360 GVAGQL D+QREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVID Sbjct: 298 GVAGQLTDSQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVID 357 Query: 361 DQRLQWLARTLTVEIEGAARTVVVDPEKMAIVLSNLLSNAVRFSPEGGCIRFLLAEGPGV 420 DQRLQWLAR L VEIEGAARTVVVDPEKMAIVL+NLLSNAVRFSP+GG IRFLL+E PG+ Sbjct: 358 DQRLQWLARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGL 417 Query: 421 VRVECIDQGPGVAPTDAARIFEPFYQGLHQPTGARRGNGIGLSVVREYVLAHSGKVYLVP 480 VRVEC+DQGPGVAPTDAARIFEPFYQGL+QPT ARRGNGIGLSVVREYV AHSGKVYLVP Sbjct: 418 VRVECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVP 477 Query: 481 REGGAHFRIELPDEK 495 REGGAHFRIELPDEK Sbjct: 478 REGGAHFRIELPDEK 492 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 82/287 (28%), Positives = 129/287 (44%), Gaps = 10/287 (3%) Query: 195 QRQLLTAQVLGAIVLAVLLAFC---FGLWLSRPMARLEQAIGRLGDNRFDQPIEVRGPAD 251 Q+ AQ++ A+V +L AF L+RP+ R+ +A RL + +EV + Sbjct: 167 QQSQFAAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDE 226 Query: 252 IRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLNDNQR 311 + L Q + L L E + F+ VSHEL+TPLA L L+DGV L + + Sbjct: 227 VGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGV---LQPDAQ 283 Query: 312 EIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHK--VIDDQRLQWLAR 369 + R L+ + L + DL + A +DL LL + R Sbjct: 284 TL-RSLKSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGM 342 Query: 370 TLTVEIEGAARTVVVDPEKMAIVLSNLLSNAVRFSPEGGCIRFLLAEGPGVVRVECIDQG 429 L+ E+ A+ V D ++ +LSNL N++R++ GG ++ + D Sbjct: 343 RLSCELP-ASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSE 401 Query: 430 PGVAPTDAARIFEPFYQGLHQPTGARRGNGIGLSVVREYVLAHSGKV 476 PGV P AR+FE F++ A G G+GL++ R V AH G + Sbjct: 402 PGVPPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTI 448 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 84/303 (27%), Positives = 136/303 (44%), Gaps = 24/303 (7%) Query: 189 SAALDQQRQLLTAQVLGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQPIEVRG 248 SAA D ++ ++ A+VL +A + +P+ + +A +LG N P+ G Sbjct: 197 SAAFDYFMRIYALRIF-AVVLIAWMAVRLAM---KPLQVMAEAAEKLGGNIRSAPLATDG 252 Query: 249 PADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLND 308 P ++ Q + +++R+ D AE++RFL VSH+L++P+ + +L D + Sbjct: 253 PLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPD-------E 305 Query: 309 NQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWLA 368 +E R++ + I L + D Q P +DL +LL + DQ Sbjct: 306 RGKE---KFRKDLDEMDALISATLNVVQ-DIDVQE-RPQLIDLDSLLESLRTDQ----AE 356 Query: 369 RTLTVEIEGAARTVVVDPEKMAIVLSNLLSNAVRFSPEGGCIRFLLAEGPGVVRVECIDQ 428 V + G A + P + L NL+ NAVR+ G R L + V + D Sbjct: 357 VGGEVTLSGHAAPLQGYPRSLRRCLQNLVENAVRY---GQRARVQLQDDADQVCIRVDDD 413 Query: 429 GPGVAPTDAARIFEPFYQGLHQPTGARRGNGIGLSVVREYVLAHSGKVYLVPREGGAHFR 488 GPG+ R+ EPFY+ G G+GLS+ V AH G + LV R GG R Sbjct: 414 GPGIPEALLERVQEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGG-LR 472 Query: 489 IEL 491 +E+ Sbjct: 473 VEI 475 >gi|300313022|ref|YP_003777114.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 82.4 bits (202), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 68/238 (28%), Positives = 113/238 (47%), Gaps = 11/238 (4%) Query: 262 LRQRLADLEAE--KSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLNDNQ-REIARILR 318 +R R L+A KSRFL ++SHE +TPL G D + +L ++ + A + Sbjct: 318 VRSRREALQANQAKSRFLSNMSHEFRTPL----NGVLGYSDALREELGESPLAQYAVAIH 373 Query: 319 QNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWLARTLTVEI--- 375 + L + +L E+ +H P ++R L+ + I Q A+ L +E Sbjct: 374 DSGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQAQAKGLPLECRIG 433 Query: 376 EGAARTVVVDPEKMAIVLSNLLSNAVRFSPEGGCIRFLLAEGPGVVRVECIDQGPGVAPT 435 E + +V D K+ VL NL+SNAVR++ G + + GP + + D G G+A + Sbjct: 434 EDVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGPQQLTMRIKDTGIGIAAS 493 Query: 436 DAARIFEPFYQGLHQPTGARRGNGIGLSVVREYVLAHSGKVYLVPREG-GAHFRIELP 492 IFE F Q P ++ G G+GL++ + V+ G++ L + G+ F LP Sbjct: 494 QQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQHQGSTFSFTLP 551 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 82.0 bits (201), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 65/227 (28%), Positives = 104/227 (45%), Gaps = 10/227 (4%) Query: 273 KSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLNDNQREIARILRQNTQSLQTQIEDLL 332 KS FL ++SHEL+TPL + A LL D G L+D Q A+ + L I D+L Sbjct: 475 KSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISSAGNDLLALINDIL 534 Query: 333 RYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWLARTLTVEIE---GAARTVVVDPEKM 389 +++ I P L L ++ + + L ++E G + + D +++ Sbjct: 535 DLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQRMETDIQRL 594 Query: 390 AIVLSNLLSNAVRFSPEGGCIRFLLAEGPGVVRVECIDQGPGVAPTDAARIFEPFYQG-- 447 +L NLLSNA++F+ +G + + G + D G G+ IFE F Q Sbjct: 595 GQILKNLLSNAIKFTEKGEVSLRIYPDAGGRIAFAVRDTGIGIGEDQHQFIFEAFRQADG 654 Query: 448 -LHQPTGARRGNGIGLSVVREYVLAHSGKVYLVPREG-GAHFRIELP 492 H+ G G G+GLS+ R+ G + + R G G+ F + LP Sbjct: 655 STHRKYG---GTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLP 698 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,431,992 Number of extensions: 59101 Number of successful extensions: 393 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 339 Number of HSP's successfully gapped: 37 Length of query: 495 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 406 Effective length of database: 1,198,455 Effective search space: 486572730 Effective search space used: 486572730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)