BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4602.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 701 0.0 gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 117 1e-27 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 88 2e-18 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 67 2e-12 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 65 9e-12 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 701 bits (1809), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/405 (92%), Positives = 390/405 (96%), Gaps = 1/405 (0%) Query: 1 MHKQDTSLSTGKFPPWLAVLAALTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFL 60 M DTSLSTGKFP WLAVLA LTAMGALAIDMYLPSFTAIAQDLGVGTN VQLTLA+FL Sbjct: 1 MQNNDTSLSTGKFPAWLAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFL 60 Query: 61 IGLAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAG 120 IG+AVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLC FAQNIETLIVLRLFQGLGGSAG Sbjct: 61 IGVAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAG 120 Query: 121 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGL 180 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFA+WRGIFVVLTLFGL Sbjct: 121 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGL 180 Query: 181 LCMVGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSFVLCGGLVQAGMFAYIAG 240 LC++GTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMS+VLCGGLVQAGMFAYIAG Sbjct: 181 LCILGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAG 240 Query: 241 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQINARLVVKRSAERVLGKVLWVPAGLALM 300 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQ+NARLVVKRSAERVLGKVLWVPAGL+L Sbjct: 241 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQVNARLVVKRSAERVLGKVLWVPAGLSLT 300 Query: 301 VALLVLAGLESLPLLLFGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGTLQFGMG 360 V+LLVL GLE+LPLLL GFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMG+LQFGMG Sbjct: 301 VSLLVLVGLETLPLLLLGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGSLQFGMG 360 Query: 361 VLSGVVMSLWHDGTALPLVSVMAFCGISALLLYHFVAKHEP-VTP 404 VLSGV MSLWHDGTALPLVSVMA CG++ALL+YHF AKH P V+P Sbjct: 361 VLSGVAMSLWHDGTALPLVSVMAVCGVTALLVYHFAAKHAPAVSP 405 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 117 bits (294), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 88/294 (29%), Positives = 146/294 (49%), Gaps = 8/294 (2%) Query: 17 LAVLAALTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDR 76 L ++ A + G LAID+Y+P+ +A D +Q ++ +LI A GQ+ +GPL+D Sbjct: 23 LFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADA 82 Query: 77 FGRKPPLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEG 136 +GRK L G+ L+ + +L A N+ET + R+ QG G +A ++ +A++ D + Sbjct: 83 YGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQA 142 Query: 137 SAKAFSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTETVDV 196 A++ + + +GLAPI+AP IG + WR V L ++ L +Y Sbjct: 143 LVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRYRETLAQP 202 Query: 197 SRAEPLHLGRTLRQYWGLLRHRQFMSFVLCGGLVQAGMFAYIAGGPFVVIELHGVKPQYF 256 R P L R R +L F S L GL + + + GPF+V P +F Sbjct: 203 VRLHPATLARNARM---VLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHQPPTFF 259 Query: 257 GFVFASNAIGLIA-ASQINARLVVKR--SAERVLGKVLWVPAGLALMVALLVLA 307 G++ + AIG++ I+ R+ +R +AE+ L V G A ++ LL L+ Sbjct: 260 GYL--ALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAFVMLLLALS 311 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 59/202 (29%), Positives = 99/202 (49%), Gaps = 1/202 (0%) Query: 3 KQDTSLSTGKFPPWLAVLAALTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFLIG 62 D SL+ + ++AA M L + + +AQ GVG N + L + ++++ Sbjct: 19 TNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLT 78 Query: 63 LAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAGMV 122 LAV G ++DRFG + IA++ + S+LC F+QN+ R+ QGLGG+ + Sbjct: 79 LAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVP 138 Query: 123 IPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGLLC 182 + R +V G + KA + + +AP++ P IGG + +A+W IF++ FGL Sbjct: 139 VGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAA 198 Query: 183 MVGTRKYLTETVDVSRAEPLHL 204 + VS+ PL L Sbjct: 199 FTAVMMVVRNEHGVSK-RPLDL 219 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 48/175 (27%), Positives = 90/175 (51%), Gaps = 4/175 (2%) Query: 22 ALTAMGALAIDMYLPSFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDRFGRKP 81 A+T + + + LP+ +A+DLG + ++ ++S+++ A + G +DR+GR+ Sbjct: 46 AMTVLDTNVVGIILPT---VARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRR 102 Query: 82 PLFFGIALYFVCSLLCSFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGS-AKA 140 L GIAL+ + SL C+ A + + L R QG+G + + A++ E +A Sbjct: 103 VLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQRERA 162 Query: 141 FSMLMLVFGLAPILAPFIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTETVD 195 +++ + GL +LAP IGG++ WR F V LL V +++ E+ + Sbjct: 163 WALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRN 217 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 44/168 (26%), Positives = 81/168 (48%), Gaps = 1/168 (0%) Query: 37 SFTAIAQDLGVGTNLVQLTLASFLIGLAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLL 96 S A+A D+ V ++L L S+L+ LAV G ++DRFG I ++ + S+L Sbjct: 9 SLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAICVFMLGSIL 68 Query: 97 CSFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAP 156 C + + + R QG+GG+ + + R ++ + KA S L + P+L P Sbjct: 69 CGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGP 128 Query: 157 FIGGLMLVFANWRGIFVVLTLFGLLCMVGTRKYLTETVDVSRAEPLHL 204 +GG + + +WR IF + +L + +++ + + + PL L Sbjct: 129 PLGGFITTYFHWRWIFFINIPISILGLYLATRFIPD-LKAEQVAPLDL 175 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.144 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,187,892 Number of extensions: 49545 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 329 Number of HSP's successfully gapped: 26 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)